Aminobacter sp. J15

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Aminobacter; unclassified Aminobacter

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3999 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562F9V1|A0A562F9V1_9RHIZ CysZ protein OS=Aminobacter sp. J15 OX=935260 GN=L611_000300000600 PE=4 SV=1
MM1 pKa = 7.54TGMDD5 pKa = 4.55RR6 pKa = 11.84ISEE9 pKa = 4.41EE10 pKa = 3.62IAHH13 pKa = 6.91FIGMFNITVEE23 pKa = 4.15QARR26 pKa = 11.84LRR28 pKa = 11.84DD29 pKa = 3.76AYY31 pKa = 10.93HH32 pKa = 6.78EE33 pKa = 4.54FNPLPAVPSNPEE45 pKa = 3.97LPEE48 pKa = 5.03APASDD53 pKa = 4.46FAAPHH58 pKa = 6.01TLLGYY63 pKa = 10.55DD64 pKa = 3.06PGMRR68 pKa = 11.84YY69 pKa = 9.45APPGWSLEE77 pKa = 4.24EE78 pKa = 3.89VSPWLPLKK86 pKa = 10.66YY87 pKa = 10.09KK88 pKa = 9.07YY89 pKa = 10.89FKK91 pKa = 9.93MQAPGQYY98 pKa = 10.09PFGHH102 pKa = 6.5PQGPSPEE109 pKa = 4.76PGSGMNINITLPEE122 pKa = 4.59IDD124 pKa = 4.4PPGSVASYY132 pKa = 9.86IVQNIYY138 pKa = 10.93LSDD141 pKa = 3.4NDD143 pKa = 3.69YY144 pKa = 11.26FGVGGHH150 pKa = 5.99GLKK153 pKa = 10.73FSPQPIDD160 pKa = 3.96NGDD163 pKa = 4.52LINLALEE170 pKa = 4.46ALSISPIGSHH180 pKa = 5.86EE181 pKa = 4.2MPSGAEE187 pKa = 4.01AIKK190 pKa = 10.91DD191 pKa = 4.33FITSVAEE198 pKa = 3.69EE199 pKa = 4.6LEE201 pKa = 4.39NYY203 pKa = 9.15TPDD206 pKa = 3.25TDD208 pKa = 4.14GPAEE212 pKa = 3.75IHH214 pKa = 5.91VFSGKK219 pKa = 8.11TLDD222 pKa = 4.26GIYY225 pKa = 10.98VNGKK229 pKa = 9.36LVTEE233 pKa = 4.88APNIDD238 pKa = 4.85DD239 pKa = 3.69YY240 pKa = 11.9HH241 pKa = 8.26SFDD244 pKa = 3.91PEE246 pKa = 3.84EE247 pKa = 5.4AEE249 pKa = 4.4EE250 pKa = 4.78GSPEE254 pKa = 3.96HH255 pKa = 7.17KK256 pKa = 9.93PIKK259 pKa = 9.49NVKK262 pKa = 7.71ITYY265 pKa = 8.87DD266 pKa = 2.83GHH268 pKa = 4.93MQIEE272 pKa = 4.38ASVSISAGGNTVVNDD287 pKa = 3.88AVLTNIWTGGTVTVVAGDD305 pKa = 3.85YY306 pKa = 9.75FQLDD310 pKa = 3.4AVIQINAICDD320 pKa = 3.75EE321 pKa = 4.62DD322 pKa = 4.32TLSPTITNWSVDD334 pKa = 3.28DD335 pKa = 3.85ATNQFFNIATFKK347 pKa = 9.57HH348 pKa = 5.72TNPLDD353 pKa = 4.24NNDD356 pKa = 3.71GAHH359 pKa = 6.81LSGSPDD365 pKa = 3.41FPSYY369 pKa = 10.39WIVTEE374 pKa = 3.82ITGDD378 pKa = 3.78LLIVNWLDD386 pKa = 3.3QYY388 pKa = 11.4IFQSDD393 pKa = 3.61NDD395 pKa = 3.73VGIVSSSGVTTEE407 pKa = 5.51IIAGDD412 pKa = 3.76NLAVNTISIMEE423 pKa = 4.57LAYY426 pKa = 10.25AYY428 pKa = 9.97DD429 pKa = 3.99IIVIGGSWYY438 pKa = 10.07DD439 pKa = 3.42ANIIQQLNVLYY450 pKa = 10.92DD451 pKa = 3.5NDD453 pKa = 5.37LIGAVNGFQTTGEE466 pKa = 4.41GSVSTSGNLLWNEE479 pKa = 3.57ALIYY483 pKa = 10.44SVSGDD488 pKa = 3.8YY489 pKa = 11.54GDD491 pKa = 4.5MPAHH495 pKa = 5.2YY496 pKa = 10.11QEE498 pKa = 5.47LADD501 pKa = 4.26NLAAGKK507 pKa = 9.6EE508 pKa = 4.1VLPSGVLSDD517 pKa = 3.9PALAGNEE524 pKa = 4.06ALRR527 pKa = 11.84VLYY530 pKa = 10.43IEE532 pKa = 5.38GDD534 pKa = 4.41LINLNYY540 pKa = 9.51IRR542 pKa = 11.84QTSIVGDD549 pKa = 3.66SDD551 pKa = 4.24QIALAMNEE559 pKa = 3.95FTPRR563 pKa = 11.84ADD565 pKa = 3.94AEE567 pKa = 4.27WTITTGGNALLNHH580 pKa = 6.78AAIVDD585 pKa = 4.42LDD587 pKa = 3.88SLGNIHH593 pKa = 6.87VGGEE597 pKa = 4.14QYY599 pKa = 10.71SQAMLVQAEE608 pKa = 4.85LISDD612 pKa = 3.82KK613 pKa = 11.24PEE615 pKa = 3.84FWAQDD620 pKa = 3.15PDD622 pKa = 3.84ALVNEE627 pKa = 4.75AVAFLDD633 pKa = 5.13DD634 pKa = 5.52SIDD637 pKa = 4.76DD638 pKa = 4.57GPDD641 pKa = 4.28DD642 pKa = 3.88IPAYY646 pKa = 8.74YY647 pKa = 10.28QPDD650 pKa = 3.56NEE652 pKa = 4.49APGGDD657 pKa = 3.95GLQAMLGG664 pKa = 3.68

Molecular weight: 71.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562ERA1|A0A562ERA1_9RHIZ O-phosphoserine phosphohydrolase OS=Aminobacter sp. J15 OX=935260 GN=L611_000700000540 PE=3 SV=1
MM1 pKa = 6.74XHH3 pKa = 7.36LSLRR7 pKa = 11.84PKK9 pKa = 10.65SRR11 pKa = 11.84LQLTNPHH18 pKa = 6.33HH19 pKa = 6.72RR20 pKa = 11.84LKK22 pKa = 10.93NPPNNRR28 pKa = 11.84LRR30 pKa = 11.84NNPRR34 pKa = 11.84LKK36 pKa = 10.25RR37 pKa = 11.84QPHH40 pKa = 6.19HH41 pKa = 6.27PMKK44 pKa = 10.16PRR46 pKa = 11.84HH47 pKa = 5.48QRR49 pKa = 11.84LLLKK53 pKa = 10.09QHH55 pKa = 6.4LRR57 pKa = 11.84MPLLSLRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84PRR68 pKa = 11.84KK69 pKa = 9.03PRR71 pKa = 11.84KK72 pKa = 8.86QRR74 pKa = 11.84LRR76 pKa = 11.84QLPRR80 pKa = 11.84PMPLHH85 pKa = 6.36LRR87 pKa = 11.84QEE89 pKa = 4.08KK90 pKa = 9.76RR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84HH95 pKa = 5.49LPRR98 pKa = 11.84KK99 pKa = 7.24PRR101 pKa = 11.84RR102 pKa = 11.84VARR105 pKa = 11.84LLLRR109 pKa = 11.84NNPLLQQGSRR119 pKa = 11.84KK120 pKa = 9.41ARR122 pKa = 11.84RR123 pKa = 11.84PLLHH127 pKa = 6.89LHH129 pKa = 6.36LQHH132 pKa = 6.95PPLPRR137 pKa = 11.84RR138 pKa = 11.84ALLLRR143 pKa = 11.84QKK145 pKa = 11.26ASLQLRR151 pKa = 11.84KK152 pKa = 10.11APANRR157 pKa = 11.84LATRR161 pKa = 11.84LRR163 pKa = 11.84CSTALRR169 pKa = 11.84KK170 pKa = 9.61LLLPHH175 pKa = 6.14QRR177 pKa = 11.84VASQLRR183 pKa = 11.84MPPNSQHH190 pKa = 7.28RR191 pKa = 11.84LRR193 pKa = 11.84QLLPDD198 pKa = 3.21RR199 pKa = 11.84RR200 pKa = 11.84LPRR203 pKa = 11.84TSRR206 pKa = 11.84HH207 pKa = 5.4SSLSSRR213 pKa = 11.84SSSARR218 pKa = 11.84CAKK221 pKa = 10.39SKK223 pKa = 10.82VSASTISRR231 pKa = 11.84AAASAGRR238 pKa = 11.84APNRR242 pKa = 11.84SASLATARR250 pKa = 4.51

Molecular weight: 29.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3999

0

3999

1237581

20

2817

309.5

33.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.917 ± 0.046

0.783 ± 0.012

5.481 ± 0.035

6.389 ± 0.036

3.88 ± 0.028

8.352 ± 0.037

1.991 ± 0.017

5.601 ± 0.029

3.515 ± 0.029

9.842 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.018

2.766 ± 0.022

5.01 ± 0.029

3.08 ± 0.023

7.034 ± 0.041

5.45 ± 0.027

5.159 ± 0.028

7.547 ± 0.035

1.289 ± 0.016

2.307 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski