Amino acid dipepetide frequency for Aminobacter sp. J15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.948AlaAla: 15.948 ± 0.168	0.928AlaCys: 0.928 ± 0.03	6.362AlaAsp: 6.362 ± 0.073	8.222AlaGlu: 8.222 ± 0.102	4.432AlaPhe: 4.432 ± 0.065	10.096AlaGly: 10.096 ± 0.097	2.036AlaHis: 2.036 ± 0.042	6.76AlaIle: 6.76 ± 0.073	4.208AlaLys: 4.208 ± 0.07	12.315AlaLeu: 12.315 ± 0.113	3.452AlaMet: 3.452 ± 0.051	2.948AlaAsn: 2.948 ± 0.048	5.101AlaPro: 5.101 ± 0.086	3.676AlaGln: 3.676 ± 0.058	8.298AlaArg: 8.298 ± 0.089	6.408AlaSer: 6.408 ± 0.068	5.664AlaThr: 5.664 ± 0.078	8.297AlaVal: 8.297 ± 0.096	1.391AlaTrp: 1.391 ± 0.036	2.582AlaTyr: 2.582 ± 0.044	0.044AlaXaa: 0.044 ± 0.006
Cys	0.84CysAla: 0.84 ± 0.028	0.101CysCys: 0.101 ± 0.009	0.493CysAsp: 0.493 ± 0.02	0.44CysGlu: 0.44 ± 0.02	0.324CysPhe: 0.324 ± 0.018	0.865CysGly: 0.865 ± 0.03	0.195CysHis: 0.195 ± 0.014	0.413CysIle: 0.413 ± 0.016	0.194CysLys: 0.194 ± 0.011	0.709CysLeu: 0.709 ± 0.027	0.162CysMet: 0.162 ± 0.011	0.202CysAsn: 0.202 ± 0.013	0.412CysPro: 0.412 ± 0.02	0.203CysGln: 0.203 ± 0.013	0.557CysArg: 0.557 ± 0.021	0.448CysSer: 0.448 ± 0.019	0.373CysThr: 0.373 ± 0.017	0.601CysVal: 0.601 ± 0.022	0.106CysTrp: 0.106 ± 0.01	0.195CysTyr: 0.195 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.353AspAla: 6.353 ± 0.082	0.415AspCys: 0.415 ± 0.022	3.084AspAsp: 3.084 ± 0.067	3.977AspGlu: 3.977 ± 0.067	2.168AspPhe: 2.168 ± 0.039	4.747AspGly: 4.747 ± 0.083	1.186AspHis: 1.186 ± 0.03	3.091AspIle: 3.091 ± 0.049	1.795AspLys: 1.795 ± 0.038	5.362AspLeu: 5.362 ± 0.08	1.366AspMet: 1.366 ± 0.035	1.312AspAsn: 1.312 ± 0.039	3.257AspPro: 3.257 ± 0.057	1.585AspGln: 1.585 ± 0.038	4.141AspArg: 4.141 ± 0.069	1.977AspSer: 1.977 ± 0.041	2.544AspThr: 2.544 ± 0.056	4.148AspVal: 4.148 ± 0.06	0.844AspTrp: 0.844 ± 0.033	1.458AspTyr: 1.458 ± 0.035	0.002AspXaa: 0.002 ± 0.001
Glu	8.244GluAla: 8.244 ± 0.097	0.36GluCys: 0.36 ± 0.018	3.063GluAsp: 3.063 ± 0.052	4.355GluGlu: 4.355 ± 0.081	2.138GluPhe: 2.138 ± 0.041	4.808GluGly: 4.808 ± 0.071	1.34GluHis: 1.34 ± 0.034	3.952GluIle: 3.952 ± 0.058	2.893GluLys: 2.893 ± 0.053	6.084GluLeu: 6.084 ± 0.08	1.739GluMet: 1.739 ± 0.039	1.994GluAsn: 1.994 ± 0.035	2.977GluPro: 2.977 ± 0.068	2.301GluGln: 2.301 ± 0.049	5.258GluArg: 5.258 ± 0.084	2.376GluSer: 2.376 ± 0.046	3.577GluThr: 3.577 ± 0.056	4.503GluVal: 4.503 ± 0.069	0.793GluTrp: 0.793 ± 0.022	1.13GluTyr: 1.13 ± 0.028	0.005GluXaa: 0.005 ± 0.002
Phe	4.544PheAla: 4.544 ± 0.072	0.385PheCys: 0.385 ± 0.016	2.661PheAsp: 2.661 ± 0.047	2.347PheGlu: 2.347 ± 0.046	1.59PhePhe: 1.59 ± 0.043	3.832PheGly: 3.832 ± 0.053	0.754PheHis: 0.754 ± 0.023	2.006PheIle: 2.006 ± 0.044	1.002PheLys: 1.002 ± 0.029	3.54PheLeu: 3.54 ± 0.064	0.922PheMet: 0.922 ± 0.029	1.085PheAsn: 1.085 ± 0.032	1.715PhePro: 1.715 ± 0.033	1.033PheGln: 1.033 ± 0.032	2.485PheArg: 2.485 ± 0.049	2.363PheSer: 2.363 ± 0.044	2.01PheThr: 2.01 ± 0.045	2.998PheVal: 2.998 ± 0.05	0.557PheTrp: 0.557 ± 0.026	0.965PheTyr: 0.965 ± 0.03	0.003PheXaa: 0.003 ± 0.002
Gly	8.446GlyAla: 8.446 ± 0.097	0.773GlyCys: 0.773 ± 0.027	4.186GlyAsp: 4.186 ± 0.068	5.201GlyGlu: 5.201 ± 0.067	3.638GlyPhe: 3.638 ± 0.058	7.055GlyGly: 7.055 ± 0.102	1.8GlyHis: 1.8 ± 0.035	5.062GlyIle: 5.062 ± 0.073	3.611GlyLys: 3.611 ± 0.06	8.311GlyLeu: 8.311 ± 0.085	2.448GlyMet: 2.448 ± 0.044	2.353GlyAsn: 2.353 ± 0.053	3.207GlyPro: 3.207 ± 0.054	2.595GlyGln: 2.595 ± 0.052	5.798GlyArg: 5.798 ± 0.072	4.743GlySer: 4.743 ± 0.07	4.469GlyThr: 4.469 ± 0.066	6.163GlyVal: 6.163 ± 0.081	1.252GlyTrp: 1.252 ± 0.035	2.379GlyTyr: 2.379 ± 0.046	0.022GlyXaa: 0.022 ± 0.005
His	2.14HisAla: 2.14 ± 0.044	0.21HisCys: 0.21 ± 0.013	1.16HisAsp: 1.16 ± 0.035	1.138HisGlu: 1.138 ± 0.033	0.862HisPhe: 0.862 ± 0.028	1.865HisGly: 1.865 ± 0.041	0.596HisHis: 0.596 ± 0.03	0.966HisIle: 0.966 ± 0.027	0.519HisLys: 0.519 ± 0.021	2.014HisLeu: 2.014 ± 0.044	0.485HisMet: 0.485 ± 0.021	0.48HisAsn: 0.48 ± 0.023	1.325HisPro: 1.325 ± 0.035	0.616HisGln: 0.616 ± 0.021	1.339HisArg: 1.339 ± 0.035	0.974HisSer: 0.974 ± 0.029	0.791HisThr: 0.791 ± 0.029	1.598HisVal: 1.598 ± 0.038	0.269HisTrp: 0.269 ± 0.017	0.557HisTyr: 0.557 ± 0.019	0.002HisXaa: 0.002 ± 0.001
Ile	7.52IleAla: 7.52 ± 0.078	0.564IleCys: 0.564 ± 0.02	3.74IleAsp: 3.74 ± 0.055	4.073IleGlu: 4.073 ± 0.064	1.972IlePhe: 1.972 ± 0.048	5.066IleGly: 5.066 ± 0.068	0.974IleHis: 0.974 ± 0.032	2.893IleIle: 2.893 ± 0.052	1.49IleLys: 1.49 ± 0.036	4.765IleLeu: 4.765 ± 0.072	1.168IleMet: 1.168 ± 0.03	1.616IleAsn: 1.616 ± 0.033	2.442IlePro: 2.442 ± 0.046	1.275IleGln: 1.275 ± 0.032	3.66IleArg: 3.66 ± 0.053	3.369IleSer: 3.369 ± 0.054	2.743IleThr: 2.743 ± 0.049	4.8IleVal: 4.8 ± 0.076	0.644IleTrp: 0.644 ± 0.024	1.24IleTyr: 1.24 ± 0.032	0.002IleXaa: 0.002 ± 0.001
Lys	4.429LysAla: 4.429 ± 0.068	0.155LysCys: 0.155 ± 0.011	1.774LysAsp: 1.774 ± 0.036	1.936LysGlu: 1.936 ± 0.047	1.059LysPhe: 1.059 ± 0.03	2.746LysGly: 2.746 ± 0.051	0.63LysHis: 0.63 ± 0.024	1.971LysIle: 1.971 ± 0.044	1.53LysLys: 1.53 ± 0.041	3.664LysLeu: 3.664 ± 0.054	0.858LysMet: 0.858 ± 0.024	0.987LysAsn: 0.987 ± 0.029	2.171LysPro: 2.171 ± 0.046	1.121LysGln: 1.121 ± 0.028	2.528LysArg: 2.528 ± 0.046	1.792LysSer: 1.792 ± 0.037	1.89LysThr: 1.89 ± 0.04	2.785LysVal: 2.785 ± 0.052	0.406LysTrp: 0.406 ± 0.02	0.713LysTyr: 0.713 ± 0.028	0.007LysXaa: 0.007 ± 0.002
Leu	12.731LeuAla: 12.731 ± 0.129	0.772LeuCys: 0.772 ± 0.024	5.621LeuAsp: 5.621 ± 0.079	5.777LeuGlu: 5.777 ± 0.07	3.758LeuPhe: 3.758 ± 0.06	7.94LeuGly: 7.94 ± 0.101	1.742LeuHis: 1.742 ± 0.04	5.332LeuIle: 5.332 ± 0.075	3.757LeuLys: 3.757 ± 0.061	9.09LeuLeu: 9.09 ± 0.128	2.433LeuMet: 2.433 ± 0.05	2.605LeuAsn: 2.605 ± 0.046	5.245LeuPro: 5.245 ± 0.07	2.679LeuGln: 2.679 ± 0.047	6.568LeuArg: 6.568 ± 0.087	6.335LeuSer: 6.335 ± 0.084	5.271LeuThr: 5.271 ± 0.066	7.592LeuVal: 7.592 ± 0.088	1.062LeuTrp: 1.062 ± 0.03	2.094LeuTyr: 2.094 ± 0.043	0.013LeuXaa: 0.013 ± 0.003
Met	3.045MetAla: 3.045 ± 0.05	0.169MetCys: 0.169 ± 0.013	1.084MetAsp: 1.084 ± 0.027	1.273MetGlu: 1.273 ± 0.034	0.778MetPhe: 0.778 ± 0.023	1.87MetGly: 1.87 ± 0.045	0.435MetHis: 0.435 ± 0.02	1.5MetIle: 1.5 ± 0.035	1.076MetLys: 1.076 ± 0.03	2.69MetLeu: 2.69 ± 0.055	0.719MetMet: 0.719 ± 0.024	0.949MetAsn: 0.949 ± 0.031	1.584MetPro: 1.584 ± 0.042	0.866MetGln: 0.866 ± 0.03	1.95MetArg: 1.95 ± 0.041	1.745MetSer: 1.745 ± 0.037	1.827MetThr: 1.827 ± 0.04	1.783MetVal: 1.783 ± 0.036	0.226MetTrp: 0.226 ± 0.015	0.325MetTyr: 0.325 ± 0.017	0.002MetXaa: 0.002 ± 0.001
Asn	3.23AsnAla: 3.23 ± 0.057	0.23AsnCys: 0.23 ± 0.014	1.509AsnAsp: 1.509 ± 0.039	1.502AsnGlu: 1.502 ± 0.035	1.064AsnPhe: 1.064 ± 0.032	2.61AsnGly: 2.61 ± 0.047	0.512AsnHis: 0.512 ± 0.018	1.502AsnIle: 1.502 ± 0.038	0.756AsnLys: 0.756 ± 0.024	2.645AsnLeu: 2.645 ± 0.054	0.684AsnMet: 0.684 ± 0.024	0.759AsnAsn: 0.759 ± 0.026	1.95AsnPro: 1.95 ± 0.044	0.824AsnGln: 0.824 ± 0.028	1.94AsnArg: 1.94 ± 0.038	1.324AsnSer: 1.324 ± 0.036	1.28AsnThr: 1.28 ± 0.035	2.167AsnVal: 2.167 ± 0.045	0.461AsnTrp: 0.461 ± 0.019	0.703AsnTyr: 0.703 ± 0.021	0.003AsnXaa: 0.003 ± 0.001
Pro	5.971ProAla: 5.971 ± 0.085	0.292ProCys: 0.292 ± 0.016	3.339ProAsp: 3.339 ± 0.049	3.98ProGlu: 3.98 ± 0.063	2.05ProPhe: 2.05 ± 0.039	4.068ProGly: 4.068 ± 0.066	1.097ProHis: 1.097 ± 0.032	2.343ProIle: 2.343 ± 0.04	1.766ProLys: 1.766 ± 0.044	4.641ProLeu: 4.641 ± 0.063	1.178ProMet: 1.178 ± 0.037	1.47ProAsn: 1.47 ± 0.034	2.099ProPro: 2.099 ± 0.051	1.802ProGln: 1.802 ± 0.042	2.888ProArg: 2.888 ± 0.054	2.752ProSer: 2.752 ± 0.044	2.295ProThr: 2.295 ± 0.045	4.204ProVal: 4.204 ± 0.065	0.637ProTrp: 0.637 ± 0.022	1.214ProTyr: 1.214 ± 0.033	0.01ProXaa: 0.01 ± 0.003
Gln	3.616GlnAla: 3.616 ± 0.058	0.183GlnCys: 0.183 ± 0.012	1.401GlnAsp: 1.401 ± 0.037	1.771GlnGlu: 1.771 ± 0.039	1.098GlnPhe: 1.098 ± 0.029	2.191GlnGly: 2.191 ± 0.043	0.635GlnHis: 0.635 ± 0.025	1.906GlnIle: 1.906 ± 0.04	1.2GlnLys: 1.2 ± 0.03	2.965GlnLeu: 2.965 ± 0.052	0.93GlnMet: 0.93 ± 0.027	0.91GlnAsn: 0.91 ± 0.029	1.806GlnPro: 1.806 ± 0.046	1.307GlnGln: 1.307 ± 0.042	2.384GlnArg: 2.384 ± 0.044	1.634GlnSer: 1.634 ± 0.035	1.646GlnThr: 1.646 ± 0.036	2.231GlnVal: 2.231 ± 0.045	0.377GlnTrp: 0.377 ± 0.018	0.603GlnTyr: 0.603 ± 0.022	0.003GlnXaa: 0.003 ± 0.002
Arg	7.451ArgAla: 7.451 ± 0.092	0.454ArgCys: 0.454 ± 0.022	3.89ArgAsp: 3.89 ± 0.06	4.906ArgGlu: 4.906 ± 0.072	2.974ArgPhe: 2.974 ± 0.053	4.649ArgGly: 4.649 ± 0.06	1.672ArgHis: 1.672 ± 0.037	4.199ArgIle: 4.199 ± 0.059	2.652ArgLys: 2.652 ± 0.049	7.431ArgLeu: 7.431 ± 0.091	1.973ArgMet: 1.973 ± 0.045	2.031ArgAsn: 2.031 ± 0.037	3.39ArgPro: 3.39 ± 0.059	2.581ArgGln: 2.581 ± 0.045	5.618ArgArg: 5.618 ± 0.095	3.694ArgSer: 3.694 ± 0.063	3.346ArgThr: 3.346 ± 0.045	4.699ArgVal: 4.699 ± 0.069	0.967ArgTrp: 0.967 ± 0.028	1.76ArgTyr: 1.76 ± 0.036	0.006ArgXaa: 0.006 ± 0.002
Ser	6.016SerAla: 6.016 ± 0.074	0.419SerCys: 0.419 ± 0.02	2.773SerAsp: 2.773 ± 0.05	3.111SerGlu: 3.111 ± 0.053	2.412SerPhe: 2.412 ± 0.041	5.473SerGly: 5.473 ± 0.075	1.064SerHis: 1.064 ± 0.033	2.959SerIle: 2.959 ± 0.053	1.648SerLys: 1.648 ± 0.035	5.467SerLeu: 5.467 ± 0.065	1.388SerMet: 1.388 ± 0.032	1.489SerAsn: 1.489 ± 0.036	2.721SerPro: 2.721 ± 0.044	1.617SerGln: 1.617 ± 0.031	3.726SerArg: 3.726 ± 0.054	3.127SerSer: 3.127 ± 0.054	2.755SerThr: 2.755 ± 0.056	4.142SerVal: 4.142 ± 0.056	0.791SerTrp: 0.791 ± 0.026	1.386SerTyr: 1.386 ± 0.035	0.013SerXaa: 0.013 ± 0.004
Thr	5.759ThrAla: 5.759 ± 0.068	0.399ThrCys: 0.399 ± 0.021	2.558ThrAsp: 2.558 ± 0.047	2.765ThrGlu: 2.765 ± 0.049	2.075ThrPhe: 2.075 ± 0.044	5.042ThrGly: 5.042 ± 0.069	1.029ThrHis: 1.029 ± 0.032	3.162ThrIle: 3.162 ± 0.059	1.471ThrLys: 1.471 ± 0.034	5.343ThrLeu: 5.343 ± 0.071	1.203ThrMet: 1.203 ± 0.032	1.351ThrAsn: 1.351 ± 0.035	3.091ThrPro: 3.091 ± 0.056	1.387ThrGln: 1.387 ± 0.037	3.239ThrArg: 3.239 ± 0.05	2.917ThrSer: 2.917 ± 0.048	2.709ThrThr: 2.709 ± 0.05	4.228ThrVal: 4.228 ± 0.072	0.619ThrTrp: 0.619 ± 0.021	1.235ThrTyr: 1.235 ± 0.036	0.007ThrXaa: 0.007 ± 0.003
Val	9.228ValAla: 9.228 ± 0.092	0.647ValCys: 0.647 ± 0.023	4.034ValAsp: 4.034 ± 0.057	5.123ValGlu: 5.123 ± 0.077	2.893ValPhe: 2.893 ± 0.054	5.632ValGly: 5.632 ± 0.079	1.381ValHis: 1.381 ± 0.029	4.303ValIle: 4.303 ± 0.068	2.413ValLys: 2.413 ± 0.048	7.381ValLeu: 7.381 ± 0.098	1.951ValMet: 1.951 ± 0.044	2.049ValAsn: 2.049 ± 0.045	3.816ValPro: 3.816 ± 0.059	1.992ValGln: 1.992 ± 0.042	5.078ValArg: 5.078 ± 0.06	4.509ValSer: 4.509 ± 0.074	4.486ValThr: 4.486 ± 0.063	6.007ValVal: 6.007 ± 0.085	0.894ValTrp: 0.894 ± 0.028	1.646ValTyr: 1.646 ± 0.034	0.006ValXaa: 0.006 ± 0.002
Trp	1.18TrpAla: 1.18 ± 0.035	0.132TrpCys: 0.132 ± 0.01	0.599TrpAsp: 0.599 ± 0.024	0.58TrpGlu: 0.58 ± 0.022	0.523TrpPhe: 0.523 ± 0.022	0.838TrpGly: 0.838 ± 0.026	0.319TrpHis: 0.319 ± 0.015	0.662TrpIle: 0.662 ± 0.021	0.517TrpLys: 0.517 ± 0.019	1.615TrpLeu: 1.615 ± 0.039	0.366TrpMet: 0.366 ± 0.019	0.465TrpAsn: 0.465 ± 0.021	0.662TrpPro: 0.662 ± 0.022	0.566TrpGln: 0.566 ± 0.023	1.063TrpArg: 1.063 ± 0.032	0.777TrpSer: 0.777 ± 0.025	0.713TrpThr: 0.713 ± 0.024	0.782TrpVal: 0.782 ± 0.025	0.225TrpTrp: 0.225 ± 0.014	0.3TrpTyr: 0.3 ± 0.016	0.004TrpXaa: 0.004 ± 0.002
Tyr	2.448TyrAla: 2.448 ± 0.047	0.239TyrCys: 0.239 ± 0.015	1.475TyrAsp: 1.475 ± 0.039	1.404TyrGlu: 1.404 ± 0.038	0.983TyrPhe: 0.983 ± 0.028	2.105TyrGly: 2.105 ± 0.043	0.503TyrHis: 0.503 ± 0.02	1.029TyrIle: 1.029 ± 0.031	0.655TyrLys: 0.655 ± 0.027	2.37TyrLeu: 2.37 ± 0.039	0.469TyrMet: 0.469 ± 0.017	0.6TyrAsn: 0.6 ± 0.023	1.111TyrPro: 1.111 ± 0.029	0.766TyrGln: 0.766 ± 0.026	1.812TyrArg: 1.812 ± 0.04	1.221TyrSer: 1.221 ± 0.034	1.197TyrThr: 1.197 ± 0.036	1.734TyrVal: 1.734 ± 0.038	0.366TyrTrp: 0.366 ± 0.016	0.588TyrTyr: 0.588 ± 0.02	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.032XaaAla: 0.032 ± 0.005	0.002XaaCys: 0.002 ± 0.001	0.006XaaAsp: 0.006 ± 0.002	0.009XaaGlu: 0.009 ± 0.002	0.006XaaPhe: 0.006 ± 0.002	0.018XaaGly: 0.018 ± 0.004	0.005XaaHis: 0.005 ± 0.002	0.004XaaIle: 0.004 ± 0.002	0.001XaaLys: 0.001 ± 0.001	0.016XaaLeu: 0.016 ± 0.003	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.014XaaPro: 0.014 ± 0.003	0.006XaaGln: 0.006 ± 0.002	0.012XaaArg: 0.012 ± 0.003	0.013XaaSer: 0.013 ± 0.004	0.002XaaThr: 0.002 ± 0.001	0.006XaaVal: 0.006 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.002	0.034XaaXaa: 0.034 ± 0.015

Statistics based on 3999 proteins (1237581 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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