Serratia phage vB_SspM_BZS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9V3U3|A0A7G9V3U3_9CAUD CI-like repressor protein OS=Serratia phage vB_SspM_BZS1 OX=2769334 GN=phiBZS1_p55 PE=4 SV=1
MM1 pKa = 7.34ATQISMQQLKK11 pKa = 10.36SIYY14 pKa = 7.77EE15 pKa = 3.76QHH17 pKa = 6.3GADD20 pKa = 3.19VSEE23 pKa = 4.52RR24 pKa = 11.84QLQEE28 pKa = 4.21SLEE31 pKa = 4.19QCNEE35 pKa = 3.94QADD38 pKa = 4.23VVYY41 pKa = 11.02NDD43 pKa = 3.89YY44 pKa = 11.21NGNPLPPRR52 pKa = 11.84TATEE56 pKa = 3.85WAHH59 pKa = 5.12WFARR63 pKa = 11.84GAAEE67 pKa = 3.95EE68 pKa = 4.0QLCEE72 pKa = 4.27GLAADD77 pKa = 4.46DD78 pKa = 4.21FQRR81 pKa = 11.84NAYY84 pKa = 9.92GFDD87 pKa = 3.26

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9V3S4|A0A7G9V3S4_9CAUD Uncharacterized protein OS=Serratia phage vB_SspM_BZS1 OX=2769334 GN=phiBZS1_p36 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.05PRR4 pKa = 11.84IPQRR8 pKa = 11.84ISIKK12 pKa = 10.89AEE14 pKa = 3.87GVLCAYY20 pKa = 9.71KK21 pKa = 10.49AGKK24 pKa = 7.95KK25 pKa = 8.29TPARR29 pKa = 11.84TYY31 pKa = 8.68QHH33 pKa = 6.02NHH35 pKa = 4.59LTLPVARR42 pKa = 11.84CWRR45 pKa = 11.84LLSKK49 pKa = 11.07DD50 pKa = 3.98NGHH53 pKa = 5.6SWEE56 pKa = 4.32VMSHH60 pKa = 4.79EE61 pKa = 4.79RR62 pKa = 11.84YY63 pKa = 10.12NNQIRR68 pKa = 11.84II69 pKa = 3.91

Molecular weight: 8.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14106

42

793

190.6

21.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.918 ± 0.303

1.248 ± 0.139

6.196 ± 0.302

5.983 ± 0.36

3.304 ± 0.175

7.422 ± 0.306

1.772 ± 0.156

5.133 ± 0.206

5.423 ± 0.34

8.223 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.142

4.353 ± 0.278

4.679 ± 0.229

4.183 ± 0.236

6.394 ± 0.26

6.139 ± 0.172

5.884 ± 0.274

6.48 ± 0.229

1.673 ± 0.153

2.985 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski