Amino acid dipepetide frequency for Serratia phage vB_SspM_BZS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.414AlaAla: 11.414 ± 0.902
1.489AlaCys: 1.489 ± 0.383
5.317AlaAsp: 5.317 ± 0.684
7.019AlaGlu: 7.019 ± 0.72
3.19AlaPhe: 3.19 ± 0.493
7.09AlaGly: 7.09 ± 0.726
1.56AlaHis: 1.56 ± 0.289
4.963AlaIle: 4.963 ± 0.543
5.175AlaLys: 5.175 ± 0.674
8.011AlaLeu: 8.011 ± 0.793
2.978AlaMet: 2.978 ± 0.566
4.041AlaAsn: 4.041 ± 0.433
5.034AlaPro: 5.034 ± 0.675
4.537AlaGln: 4.537 ± 0.474
4.396AlaArg: 4.396 ± 0.628
5.105AlaSer: 5.105 ± 0.463
5.601AlaThr: 5.601 ± 0.639
7.019AlaVal: 7.019 ± 0.543
1.631AlaTrp: 1.631 ± 0.282
3.616AlaTyr: 3.616 ± 0.397
0.0AlaXaa: 0.0 ± 0.0
Cys
1.063CysAla: 1.063 ± 0.294
0.213CysCys: 0.213 ± 0.142
0.993CysAsp: 0.993 ± 0.234
0.851CysGlu: 0.851 ± 0.284
0.496CysPhe: 0.496 ± 0.227
1.134CysGly: 1.134 ± 0.307
0.354CysHis: 0.354 ± 0.151
0.284CysIle: 0.284 ± 0.125
0.425CysLys: 0.425 ± 0.17
0.993CysLeu: 0.993 ± 0.257
0.213CysMet: 0.213 ± 0.121
0.071CysAsn: 0.071 ± 0.08
0.851CysPro: 0.851 ± 0.231
0.638CysGln: 0.638 ± 0.223
1.347CysArg: 1.347 ± 0.349
0.922CysSer: 0.922 ± 0.234
0.142CysThr: 0.142 ± 0.102
0.709CysVal: 0.709 ± 0.264
0.567CysTrp: 0.567 ± 0.176
0.213CysTyr: 0.213 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
6.664AspAla: 6.664 ± 0.638
0.638AspCys: 0.638 ± 0.154
5.246AspAsp: 5.246 ± 1.02
3.545AspGlu: 3.545 ± 0.511
2.269AspPhe: 2.269 ± 0.42
5.955AspGly: 5.955 ± 0.566
0.993AspHis: 0.993 ± 0.249
2.127AspIle: 2.127 ± 0.472
2.41AspLys: 2.41 ± 0.442
4.892AspLeu: 4.892 ± 0.712
1.347AspMet: 1.347 ± 0.347
2.907AspAsn: 2.907 ± 0.445
2.836AspPro: 2.836 ± 0.492
2.41AspGln: 2.41 ± 0.451
3.261AspArg: 3.261 ± 0.507
3.332AspSer: 3.332 ± 0.576
3.758AspThr: 3.758 ± 0.835
4.396AspVal: 4.396 ± 0.547
1.205AspTrp: 1.205 ± 0.346
1.772AspTyr: 1.772 ± 0.358
0.0AspXaa: 0.0 ± 0.0
Glu
5.105GluAla: 5.105 ± 0.653
0.709GluCys: 0.709 ± 0.232
2.907GluAsp: 2.907 ± 0.655
3.97GluGlu: 3.97 ± 0.655
2.623GluPhe: 2.623 ± 0.441
3.545GluGly: 3.545 ± 0.489
1.489GluHis: 1.489 ± 0.323
3.261GluIle: 3.261 ± 0.47
4.679GluLys: 4.679 ± 0.558
4.892GluLeu: 4.892 ± 0.614
2.552GluMet: 2.552 ± 0.454
2.552GluAsn: 2.552 ± 0.372
2.127GluPro: 2.127 ± 0.475
2.269GluGln: 2.269 ± 0.525
4.892GluArg: 4.892 ± 0.678
3.403GluSer: 3.403 ± 0.494
2.694GluThr: 2.694 ± 0.457
3.758GluVal: 3.758 ± 0.446
1.063GluTrp: 1.063 ± 0.322
1.347GluTyr: 1.347 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
2.907PheAla: 2.907 ± 0.472
0.425PheCys: 0.425 ± 0.162
2.978PheAsp: 2.978 ± 0.42
1.914PheGlu: 1.914 ± 0.323
1.205PhePhe: 1.205 ± 0.312
2.41PheGly: 2.41 ± 0.415
0.709PheHis: 0.709 ± 0.215
2.127PheIle: 2.127 ± 0.447
1.631PheLys: 1.631 ± 0.231
1.914PheLeu: 1.914 ± 0.356
0.638PheMet: 0.638 ± 0.203
1.631PheAsn: 1.631 ± 0.387
1.134PhePro: 1.134 ± 0.311
1.418PheGln: 1.418 ± 0.397
1.631PheArg: 1.631 ± 0.27
2.765PheSer: 2.765 ± 0.483
2.127PheThr: 2.127 ± 0.336
1.489PheVal: 1.489 ± 0.303
0.78PheTrp: 0.78 ± 0.225
1.205PheTyr: 1.205 ± 0.28
0.0PheXaa: 0.0 ± 0.0
Gly
5.743GlyAla: 5.743 ± 0.853
0.78GlyCys: 0.78 ± 0.291
4.467GlyAsp: 4.467 ± 0.638
5.246GlyGlu: 5.246 ± 0.546
2.694GlyPhe: 2.694 ± 0.424
6.877GlyGly: 6.877 ± 0.892
1.063GlyHis: 1.063 ± 0.251
4.325GlyIle: 4.325 ± 0.699
4.396GlyLys: 4.396 ± 0.57
4.467GlyLeu: 4.467 ± 0.734
2.056GlyMet: 2.056 ± 0.36
4.325GlyAsn: 4.325 ± 0.657
1.418GlyPro: 1.418 ± 0.34
2.765GlyGln: 2.765 ± 0.57
4.608GlyArg: 4.608 ± 0.592
4.679GlySer: 4.679 ± 0.656
4.254GlyThr: 4.254 ± 0.53
5.53GlyVal: 5.53 ± 0.511
1.205GlyTrp: 1.205 ± 0.263
3.332GlyTyr: 3.332 ± 0.517
0.0GlyXaa: 0.0 ± 0.0
His
1.276HisAla: 1.276 ± 0.299
0.78HisCys: 0.78 ± 0.258
1.063HisAsp: 1.063 ± 0.32
0.851HisGlu: 0.851 ± 0.22
0.425HisPhe: 0.425 ± 0.181
2.198HisGly: 2.198 ± 0.392
0.425HisHis: 0.425 ± 0.151
1.063HisIle: 1.063 ± 0.301
0.851HisLys: 0.851 ± 0.261
1.702HisLeu: 1.702 ± 0.392
0.213HisMet: 0.213 ± 0.128
0.709HisAsn: 0.709 ± 0.186
0.993HisPro: 0.993 ± 0.281
0.354HisGln: 0.354 ± 0.139
0.638HisArg: 0.638 ± 0.182
1.347HisSer: 1.347 ± 0.268
1.063HisThr: 1.063 ± 0.205
1.134HisVal: 1.134 ± 0.22
0.284HisTrp: 0.284 ± 0.138
0.354HisTyr: 0.354 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
5.672IleAla: 5.672 ± 0.65
0.567IleCys: 0.567 ± 0.177
4.254IleAsp: 4.254 ± 0.672
3.899IleGlu: 3.899 ± 0.524
1.843IlePhe: 1.843 ± 0.264
3.119IleGly: 3.119 ± 0.425
1.205IleHis: 1.205 ± 0.311
2.623IleIle: 2.623 ± 0.414
3.19IleLys: 3.19 ± 0.41
3.474IleLeu: 3.474 ± 0.496
0.851IleMet: 0.851 ± 0.231
2.623IleAsn: 2.623 ± 0.371
2.907IlePro: 2.907 ± 0.413
1.702IleGln: 1.702 ± 0.391
2.978IleArg: 2.978 ± 0.51
3.474IleSer: 3.474 ± 0.473
3.119IleThr: 3.119 ± 0.355
2.127IleVal: 2.127 ± 0.356
0.638IleTrp: 0.638 ± 0.256
1.063IleTyr: 1.063 ± 0.246
0.0IleXaa: 0.0 ± 0.0
Lys
5.246LysAla: 5.246 ± 0.606
0.78LysCys: 0.78 ± 0.294
2.907LysAsp: 2.907 ± 0.478
3.332LysGlu: 3.332 ± 0.434
1.418LysPhe: 1.418 ± 0.314
3.261LysGly: 3.261 ± 0.433
0.993LysHis: 0.993 ± 0.321
2.198LysIle: 2.198 ± 0.393
3.403LysLys: 3.403 ± 0.561
4.963LysLeu: 4.963 ± 0.544
1.985LysMet: 1.985 ± 0.354
2.694LysAsn: 2.694 ± 0.521
3.261LysPro: 3.261 ± 0.499
1.772LysGln: 1.772 ± 0.378
3.828LysArg: 3.828 ± 0.621
3.049LysSer: 3.049 ± 0.524
3.616LysThr: 3.616 ± 0.414
3.474LysVal: 3.474 ± 0.465
0.425LysTrp: 0.425 ± 0.141
1.631LysTyr: 1.631 ± 0.402
0.0LysXaa: 0.0 ± 0.0
Leu
8.579LeuAla: 8.579 ± 0.686
0.993LeuCys: 0.993 ± 0.222
4.325LeuAsp: 4.325 ± 0.525
4.112LeuGlu: 4.112 ± 0.521
2.34LeuPhe: 2.34 ± 0.459
5.034LeuGly: 5.034 ± 0.553
1.418LeuHis: 1.418 ± 0.282
4.679LeuIle: 4.679 ± 0.538
4.75LeuLys: 4.75 ± 0.524
7.09LeuLeu: 7.09 ± 0.804
1.985LeuMet: 1.985 ± 0.398
4.041LeuAsn: 4.041 ± 0.492
4.537LeuPro: 4.537 ± 0.595
3.049LeuGln: 3.049 ± 0.419
5.459LeuArg: 5.459 ± 0.521
5.884LeuSer: 5.884 ± 0.6
5.884LeuThr: 5.884 ± 0.561
5.175LeuVal: 5.175 ± 0.599
1.418LeuTrp: 1.418 ± 0.402
1.489LeuTyr: 1.489 ± 0.281
0.0LeuXaa: 0.0 ± 0.0
Met
3.616MetAla: 3.616 ± 0.577
0.071MetCys: 0.071 ± 0.066
1.418MetAsp: 1.418 ± 0.321
1.276MetGlu: 1.276 ± 0.306
0.354MetPhe: 0.354 ± 0.16
1.631MetGly: 1.631 ± 0.343
0.142MetHis: 0.142 ± 0.073
1.134MetIle: 1.134 ± 0.263
1.631MetLys: 1.631 ± 0.342
2.41MetLeu: 2.41 ± 0.413
0.496MetMet: 0.496 ± 0.166
1.134MetAsn: 1.134 ± 0.348
1.489MetPro: 1.489 ± 0.34
1.063MetGln: 1.063 ± 0.278
1.772MetArg: 1.772 ± 0.302
1.914MetSer: 1.914 ± 0.381
2.127MetThr: 2.127 ± 0.35
1.702MetVal: 1.702 ± 0.37
0.354MetTrp: 0.354 ± 0.155
0.354MetTyr: 0.354 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
4.679AsnAla: 4.679 ± 0.564
0.638AsnCys: 0.638 ± 0.237
3.545AsnAsp: 3.545 ± 0.538
1.631AsnGlu: 1.631 ± 0.338
1.063AsnPhe: 1.063 ± 0.284
4.112AsnGly: 4.112 ± 0.617
0.496AsnHis: 0.496 ± 0.195
2.34AsnIle: 2.34 ± 0.367
2.269AsnLys: 2.269 ± 0.453
3.545AsnLeu: 3.545 ± 0.519
0.922AsnMet: 0.922 ± 0.201
1.914AsnAsn: 1.914 ± 0.574
1.985AsnPro: 1.985 ± 0.43
1.843AsnGln: 1.843 ± 0.427
2.765AsnArg: 2.765 ± 0.426
2.694AsnSer: 2.694 ± 0.512
2.978AsnThr: 2.978 ± 0.495
1.985AsnVal: 1.985 ± 0.368
0.709AsnTrp: 0.709 ± 0.194
1.418AsnTyr: 1.418 ± 0.276
0.0AsnXaa: 0.0 ± 0.0
Pro
3.899ProAla: 3.899 ± 0.426
0.425ProCys: 0.425 ± 0.147
3.828ProAsp: 3.828 ± 0.605
3.19ProGlu: 3.19 ± 0.586
1.56ProPhe: 1.56 ± 0.429
3.332ProGly: 3.332 ± 0.392
1.134ProHis: 1.134 ± 0.363
1.56ProIle: 1.56 ± 0.332
2.481ProLys: 2.481 ± 0.458
3.97ProLeu: 3.97 ± 0.652
0.78ProMet: 0.78 ± 0.248
1.489ProAsn: 1.489 ± 0.32
2.552ProPro: 2.552 ± 0.587
1.631ProGln: 1.631 ± 0.272
2.198ProArg: 2.198 ± 0.401
3.049ProSer: 3.049 ± 0.413
3.19ProThr: 3.19 ± 0.476
4.325ProVal: 4.325 ± 0.588
0.496ProTrp: 0.496 ± 0.22
1.702ProTyr: 1.702 ± 0.347
0.0ProXaa: 0.0 ± 0.0
Gln
4.325GlnAla: 4.325 ± 0.691
0.354GlnCys: 0.354 ± 0.163
2.34GlnAsp: 2.34 ± 0.363
2.198GlnGlu: 2.198 ± 0.49
1.843GlnPhe: 1.843 ± 0.38
2.41GlnGly: 2.41 ± 0.38
1.134GlnHis: 1.134 ± 0.246
2.481GlnIle: 2.481 ± 0.406
1.772GlnLys: 1.772 ± 0.315
3.616GlnLeu: 3.616 ± 0.591
1.205GlnMet: 1.205 ± 0.243
1.347GlnAsn: 1.347 ± 0.369
2.481GlnPro: 2.481 ± 0.385
2.552GlnGln: 2.552 ± 0.594
3.332GlnArg: 3.332 ± 0.4
2.41GlnSer: 2.41 ± 0.381
2.481GlnThr: 2.481 ± 0.365
2.269GlnVal: 2.269 ± 0.354
0.496GlnTrp: 0.496 ± 0.232
0.78GlnTyr: 0.78 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
6.735ArgAla: 6.735 ± 0.734
0.922ArgCys: 0.922 ± 0.345
2.978ArgAsp: 2.978 ± 0.466
3.332ArgGlu: 3.332 ± 0.61
2.198ArgPhe: 2.198 ± 0.307
3.828ArgGly: 3.828 ± 0.614
1.134ArgHis: 1.134 ± 0.27
4.325ArgIle: 4.325 ± 0.498
3.828ArgLys: 3.828 ± 0.645
6.31ArgLeu: 6.31 ± 0.609
1.772ArgMet: 1.772 ± 0.347
3.19ArgAsn: 3.19 ± 0.52
2.623ArgPro: 2.623 ± 0.455
2.41ArgGln: 2.41 ± 0.36
5.175ArgArg: 5.175 ± 0.674
3.261ArgSer: 3.261 ± 0.379
2.198ArgThr: 2.198 ± 0.476
3.97ArgVal: 3.97 ± 0.497
1.418ArgTrp: 1.418 ± 0.259
2.34ArgTyr: 2.34 ± 0.372
0.0ArgXaa: 0.0 ± 0.0
Ser
5.955SerAla: 5.955 ± 0.58
0.709SerCys: 0.709 ± 0.257
3.545SerAsp: 3.545 ± 0.554
3.616SerGlu: 3.616 ± 0.438
2.056SerPhe: 2.056 ± 0.341
5.317SerGly: 5.317 ± 0.604
1.134SerHis: 1.134 ± 0.313
2.623SerIle: 2.623 ± 0.367
3.332SerLys: 3.332 ± 0.51
4.892SerLeu: 4.892 ± 0.638
2.269SerMet: 2.269 ± 0.462
2.907SerAsn: 2.907 ± 0.428
2.836SerPro: 2.836 ± 0.434
2.481SerGln: 2.481 ± 0.453
3.687SerArg: 3.687 ± 0.479
2.481SerSer: 2.481 ± 0.402
3.687SerThr: 3.687 ± 0.403
4.396SerVal: 4.396 ± 0.703
1.134SerTrp: 1.134 ± 0.23
2.34SerTyr: 2.34 ± 0.469
0.0SerXaa: 0.0 ± 0.0
Thr
6.239ThrAla: 6.239 ± 0.736
0.496ThrCys: 0.496 ± 0.204
3.616ThrAsp: 3.616 ± 0.869
2.765ThrGlu: 2.765 ± 0.376
2.34ThrPhe: 2.34 ± 0.426
5.388ThrGly: 5.388 ± 0.694
0.425ThrHis: 0.425 ± 0.217
3.403ThrIle: 3.403 ± 0.467
2.41ThrLys: 2.41 ± 0.314
5.034ThrLeu: 5.034 ± 0.786
1.063ThrMet: 1.063 ± 0.268
1.985ThrAsn: 1.985 ± 0.385
3.119ThrPro: 3.119 ± 0.486
3.049ThrGln: 3.049 ± 0.495
4.183ThrArg: 4.183 ± 0.686
3.049ThrSer: 3.049 ± 0.422
3.403ThrThr: 3.403 ± 0.534
4.396ThrVal: 4.396 ± 0.528
0.78ThrTrp: 0.78 ± 0.249
1.702ThrTyr: 1.702 ± 0.319
0.0ThrXaa: 0.0 ± 0.0
Val
5.743ValAla: 5.743 ± 0.518
0.709ValCys: 0.709 ± 0.196
3.403ValAsp: 3.403 ± 0.466
4.396ValGlu: 4.396 ± 0.514
1.914ValPhe: 1.914 ± 0.399
4.325ValGly: 4.325 ± 0.567
0.993ValHis: 0.993 ± 0.273
4.041ValIle: 4.041 ± 0.562
4.183ValLys: 4.183 ± 0.467
5.175ValLeu: 5.175 ± 0.564
1.631ValMet: 1.631 ± 0.332
2.552ValAsn: 2.552 ± 0.365
2.481ValPro: 2.481 ± 0.341
2.978ValGln: 2.978 ± 0.505
4.183ValArg: 4.183 ± 0.586
4.396ValSer: 4.396 ± 0.516
4.254ValThr: 4.254 ± 0.638
4.325ValVal: 4.325 ± 0.512
1.205ValTrp: 1.205 ± 0.226
1.843ValTyr: 1.843 ± 0.353
0.0ValXaa: 0.0 ± 0.0
Trp
1.631TrpAla: 1.631 ± 0.362
0.0TrpCys: 0.0 ± 0.0
0.78TrpAsp: 0.78 ± 0.223
1.134TrpGlu: 1.134 ± 0.28
0.354TrpPhe: 0.354 ± 0.138
1.347TrpGly: 1.347 ± 0.289
0.213TrpHis: 0.213 ± 0.118
0.78TrpIle: 0.78 ± 0.245
0.425TrpLys: 0.425 ± 0.146
2.198TrpLeu: 2.198 ± 0.42
0.354TrpMet: 0.354 ± 0.162
0.354TrpAsn: 0.354 ± 0.146
0.78TrpPro: 0.78 ± 0.24
0.78TrpGln: 0.78 ± 0.245
1.631TrpArg: 1.631 ± 0.286
1.631TrpSer: 1.631 ± 0.27
0.425TrpThr: 0.425 ± 0.152
1.205TrpVal: 1.205 ± 0.32
0.284TrpTrp: 0.284 ± 0.119
0.425TrpTyr: 0.425 ± 0.183
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.694TyrAla: 2.694 ± 0.39
0.78TyrCys: 0.78 ± 0.224
2.056TyrAsp: 2.056 ± 0.336
1.56TyrGlu: 1.56 ± 0.313
0.851TyrPhe: 0.851 ± 0.25
1.914TyrGly: 1.914 ± 0.296
0.709TyrHis: 0.709 ± 0.241
0.993TyrIle: 0.993 ± 0.297
0.993TyrLys: 0.993 ± 0.243
2.694TyrLeu: 2.694 ± 0.497
0.709TyrMet: 0.709 ± 0.194
1.063TyrAsn: 1.063 ± 0.317
1.347TyrPro: 1.347 ± 0.368
2.127TyrGln: 2.127 ± 0.388
1.985TyrArg: 1.985 ± 0.366
2.552TyrSer: 2.552 ± 0.354
1.843TyrThr: 1.843 ± 0.39
1.418TyrVal: 1.418 ± 0.304
0.638TyrTrp: 0.638 ± 0.246
0.922TyrTyr: 0.922 ± 0.247
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (14106 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski