Tateyamaria sp. ANG-S1
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3983 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4C986|A0A0B4C986_9RHOB Aspartate aminotransferase OS=Tateyamaria sp. ANG-S1 OX=1577905 GN=RA29_13010 PE=3 SV=1
MM1 pKa = 7.2 KK2 pKa = 9.99 TRR4 pKa = 11.84 ILAIGLAAALAGPALAGSLEE24 pKa = 4.26 PPAPEE29 pKa = 4.13 PAAPAPLPPAPVQSFGGDD47 pKa = 2.96 WTGGYY52 pKa = 10.56 AGLQLGYY59 pKa = 10.89 GDD61 pKa = 4.1 VDD63 pKa = 3.58 GSGAADD69 pKa = 3.92 GDD71 pKa = 4.05 DD72 pKa = 4.15 FLYY75 pKa = 10.44 GAHH78 pKa = 5.98 VGYY81 pKa = 10.32 RR82 pKa = 11.84 YY83 pKa = 10.77 DD84 pKa = 3.4 FGTFVLGGEE93 pKa = 4.45 LDD95 pKa = 3.65 YY96 pKa = 11.77 DD97 pKa = 4.34 FADD100 pKa = 3.63 IDD102 pKa = 4.37 LNGAASVDD110 pKa = 3.63 SVARR114 pKa = 11.84 LKK116 pKa = 11.12 LQGGYY121 pKa = 11.0 DD122 pKa = 3.96 FGRR125 pKa = 11.84 TLAYY129 pKa = 7.85 FTAGLAEE136 pKa = 4.29 VDD138 pKa = 3.45 TSLGSEE144 pKa = 3.72 SGEE147 pKa = 3.95 FYY149 pKa = 10.98 GIGVAYY155 pKa = 9.72 QINDD159 pKa = 3.67 RR160 pKa = 11.84 YY161 pKa = 8.6 TVGAEE166 pKa = 3.99 LLDD169 pKa = 4.54 HH170 pKa = 6.79 NFDD173 pKa = 5.56 DD174 pKa = 4.94 INGTGVDD181 pKa = 3.93 ADD183 pKa = 3.7 ATTLTVRR190 pKa = 11.84 GSINFF195 pKa = 3.49
Molecular weight: 20.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.439
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.897
Patrickios 0.744
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A0B4BRS4|A0A0B4BRS4_9RHOB Uncharacterized protein OS=Tateyamaria sp. ANG-S1 OX=1577905 GN=RA29_06115 PE=3 SV=1
MM1 pKa = 7.31 QSPGSTTVTLTRR13 pKa = 11.84 RR14 pKa = 11.84 SASAIAAASPAGPPPAIRR32 pKa = 11.84 TSGIRR37 pKa = 11.84 PRR39 pKa = 11.84 PGPAARR45 pKa = 11.84 MSVPQALRR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 LWVPRR61 pKa = 11.84 VGRR64 pKa = 11.84 ALL66 pKa = 3.5
Molecular weight: 6.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.544
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.281
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3983
0
3983
1214556
35
2088
304.9
33.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.157 ± 0.056
0.871 ± 0.012
6.498 ± 0.044
5.66 ± 0.039
3.804 ± 0.027
8.614 ± 0.046
2.128 ± 0.019
5.216 ± 0.029
3.153 ± 0.035
9.763 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.905 ± 0.023
2.71 ± 0.026
4.98 ± 0.03
3.346 ± 0.021
6.27 ± 0.04
5.029 ± 0.026
5.808 ± 0.033
7.413 ± 0.031
1.434 ± 0.018
2.243 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here