candidate division MSBL1 archaeon SCGC-AAA385M11
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 228 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A133VRT6|A0A133VRT6_9EURY Uncharacterized protein (Fragment) OS=candidate division MSBL1 archaeon SCGC-AAA385M11 OX=1698288 GN=AKJ60_00925 PE=4 SV=1
MM1 pKa = 7.45 QYY3 pKa = 11.39 LNIKK7 pKa = 9.96 IEE9 pKa = 4.14 VFHH12 pKa = 7.3 EE13 pKa = 4.74 DD14 pKa = 3.37 DD15 pKa = 5.49 LYY17 pKa = 11.87 VALCPNLNVSSYY29 pKa = 10.79 GEE31 pKa = 4.16 SVEE34 pKa = 4.17 EE35 pKa = 3.87 AKK37 pKa = 10.61 KK38 pKa = 10.8 ALIEE42 pKa = 4.06 AVEE45 pKa = 4.31 AFLEE49 pKa = 4.67 EE50 pKa = 4.35 CAEE53 pKa = 4.12 MGTLEE58 pKa = 5.59 DD59 pKa = 3.6 VLEE62 pKa = 4.28 EE63 pKa = 4.1 CGYY66 pKa = 11.24 SKK68 pKa = 11.18 VNQTWQPRR76 pKa = 11.84 QAVKK80 pKa = 10.66 EE81 pKa = 3.92 EE82 pKa = 4.22 SLAVTII88 pKa = 5.26
Molecular weight: 9.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.97
IPC2_protein 4.279
IPC_protein 4.126
Toseland 3.986
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.884
Rodwell 3.961
Grimsley 3.91
Solomon 4.037
Lehninger 3.986
Nozaki 4.164
DTASelect 4.215
Thurlkill 3.986
EMBOSS 3.897
Sillero 4.228
Patrickios 1.914
IPC_peptide 4.05
IPC2_peptide 4.215
IPC2.peptide.svr19 4.141
Protein with the highest isoelectric point:
>tr|A0A133VSL1|A0A133VSL1_9EURY Cell division protein FtsZ OS=candidate division MSBL1 archaeon SCGC-AAA385M11 OX=1698288 GN=ftsZ PE=3 SV=1
MM1 pKa = 7.38 SPNRR5 pKa = 11.84 PSRR8 pKa = 11.84 PVKK11 pKa = 10.19 RR12 pKa = 11.84 RR13 pKa = 11.84 PGLVALGRR21 pKa = 11.84 IARR24 pKa = 11.84 SLFLVILLLGLSYY37 pKa = 11.56 ALLRR41 pKa = 11.84 GYY43 pKa = 10.49 FWVTTLSCLALEE55 pKa = 4.39 TVEE58 pKa = 4.25 VRR60 pKa = 11.84 GNHH63 pKa = 6.25 RR64 pKa = 11.84 FTDD67 pKa = 3.7 QEE69 pKa = 4.06 IMSLAGVEE77 pKa = 4.47 VGDD80 pKa = 3.65 NCLAISLGAVKK91 pKa = 10.23 RR92 pKa = 11.84 RR93 pKa = 11.84 LSANPWLEE101 pKa = 3.99 DD102 pKa = 3.28 VLVRR106 pKa = 11.84 RR107 pKa = 11.84 VLPDD111 pKa = 3.15 RR112 pKa = 11.84 LEE114 pKa = 3.79 IAVRR118 pKa = 11.84 EE119 pKa = 4.09 KK120 pKa = 10.84 QAVFWIRR127 pKa = 11.84 HH128 pKa = 5.43 RR129 pKa = 11.84 EE130 pKa = 3.87 DD131 pKa = 3.57 LYY133 pKa = 11.17 YY134 pKa = 10.92 AQADD138 pKa = 3.86 GRR140 pKa = 11.84 PIAPVEE146 pKa = 4.14 AEE148 pKa = 4.31 NFASLPLLTLPEE160 pKa = 4.4 SNRR163 pKa = 11.84 ASARR167 pKa = 11.84 EE168 pKa = 3.84 KK169 pKa = 10.76 LNQVVKK175 pKa = 10.33 WFTRR179 pKa = 11.84 KK180 pKa = 8.7 QAPFSLAEE188 pKa = 4.07 LAWVRR193 pKa = 11.84 FRR195 pKa = 11.84 RR196 pKa = 11.84 DD197 pKa = 3.91 FILEE201 pKa = 4.15 LYY203 pKa = 9.84 LRR205 pKa = 11.84 NDD207 pKa = 3.33 RR208 pKa = 11.84 GRR210 pKa = 11.84 IRR212 pKa = 11.84 VGLEE216 pKa = 3.58 SLKK219 pKa = 11.09 EE220 pKa = 3.94 NLTVLTKK227 pKa = 9.86 VWRR230 pKa = 11.84 DD231 pKa = 3.08 LEE233 pKa = 4.04 QRR235 pKa = 11.84 RR236 pKa = 11.84 EE237 pKa = 4.05 LRR239 pKa = 11.84 EE240 pKa = 3.44 MAKK243 pKa = 9.62 IVVYY247 pKa = 10.86 QRR249 pKa = 11.84 MGWVGFSAGG258 pKa = 3.51
Molecular weight: 29.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.502
IPC_protein 10.35
Toseland 10.496
ProMoST 10.233
Dawson 10.613
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.73
Grimsley 10.672
Solomon 10.73
Lehninger 10.687
Nozaki 10.482
DTASelect 10.35
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.555
Patrickios 10.409
IPC_peptide 10.73
IPC2_peptide 9.326
IPC2.peptide.svr19 8.695
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
228
0
228
43957
27
750
192.8
22.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.584 ± 0.149
1.253 ± 0.062
5.276 ± 0.125
7.421 ± 0.202
4.655 ± 0.139
6.406 ± 0.156
2.248 ± 0.077
6.424 ± 0.126
6.795 ± 0.185
10.005 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.085
4.084 ± 0.118
4.177 ± 0.11
4.259 ± 0.113
6.242 ± 0.152
6.142 ± 0.13
4.411 ± 0.09
5.892 ± 0.129
1.638 ± 0.089
3.638 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here