candidate division MSBL1 archaeon SCGC-AAA385M11

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A133VRT6|A0A133VRT6_9EURY Uncharacterized protein (Fragment) OS=candidate division MSBL1 archaeon SCGC-AAA385M11 OX=1698288 GN=AKJ60_00925 PE=4 SV=1
MM1 pKa = 7.45QYY3 pKa = 11.39LNIKK7 pKa = 9.96IEE9 pKa = 4.14VFHH12 pKa = 7.3EE13 pKa = 4.74DD14 pKa = 3.37DD15 pKa = 5.49LYY17 pKa = 11.87VALCPNLNVSSYY29 pKa = 10.79GEE31 pKa = 4.16SVEE34 pKa = 4.17EE35 pKa = 3.87AKK37 pKa = 10.61KK38 pKa = 10.8ALIEE42 pKa = 4.06AVEE45 pKa = 4.31AFLEE49 pKa = 4.67EE50 pKa = 4.35CAEE53 pKa = 4.12MGTLEE58 pKa = 5.59DD59 pKa = 3.6VLEE62 pKa = 4.28EE63 pKa = 4.1CGYY66 pKa = 11.24SKK68 pKa = 11.18VNQTWQPRR76 pKa = 11.84QAVKK80 pKa = 10.66EE81 pKa = 3.92EE82 pKa = 4.22SLAVTII88 pKa = 5.26

Molecular weight:
9.94 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A133VSL1|A0A133VSL1_9EURY Cell division protein FtsZ OS=candidate division MSBL1 archaeon SCGC-AAA385M11 OX=1698288 GN=ftsZ PE=3 SV=1
MM1 pKa = 7.38SPNRR5 pKa = 11.84PSRR8 pKa = 11.84PVKK11 pKa = 10.19RR12 pKa = 11.84RR13 pKa = 11.84PGLVALGRR21 pKa = 11.84IARR24 pKa = 11.84SLFLVILLLGLSYY37 pKa = 11.56ALLRR41 pKa = 11.84GYY43 pKa = 10.49FWVTTLSCLALEE55 pKa = 4.39TVEE58 pKa = 4.25VRR60 pKa = 11.84GNHH63 pKa = 6.25RR64 pKa = 11.84FTDD67 pKa = 3.7QEE69 pKa = 4.06IMSLAGVEE77 pKa = 4.47VGDD80 pKa = 3.65NCLAISLGAVKK91 pKa = 10.23RR92 pKa = 11.84RR93 pKa = 11.84LSANPWLEE101 pKa = 3.99DD102 pKa = 3.28VLVRR106 pKa = 11.84RR107 pKa = 11.84VLPDD111 pKa = 3.15RR112 pKa = 11.84LEE114 pKa = 3.79IAVRR118 pKa = 11.84EE119 pKa = 4.09KK120 pKa = 10.84QAVFWIRR127 pKa = 11.84HH128 pKa = 5.43RR129 pKa = 11.84EE130 pKa = 3.87DD131 pKa = 3.57LYY133 pKa = 11.17YY134 pKa = 10.92AQADD138 pKa = 3.86GRR140 pKa = 11.84PIAPVEE146 pKa = 4.14AEE148 pKa = 4.31NFASLPLLTLPEE160 pKa = 4.4SNRR163 pKa = 11.84ASARR167 pKa = 11.84EE168 pKa = 3.84KK169 pKa = 10.76LNQVVKK175 pKa = 10.33WFTRR179 pKa = 11.84KK180 pKa = 8.7QAPFSLAEE188 pKa = 4.07LAWVRR193 pKa = 11.84FRR195 pKa = 11.84RR196 pKa = 11.84DD197 pKa = 3.91FILEE201 pKa = 4.15LYY203 pKa = 9.84LRR205 pKa = 11.84NDD207 pKa = 3.33RR208 pKa = 11.84GRR210 pKa = 11.84IRR212 pKa = 11.84VGLEE216 pKa = 3.58SLKK219 pKa = 11.09EE220 pKa = 3.94NLTVLTKK227 pKa = 9.86VWRR230 pKa = 11.84DD231 pKa = 3.08LEE233 pKa = 4.04QRR235 pKa = 11.84RR236 pKa = 11.84EE237 pKa = 4.05LRR239 pKa = 11.84EE240 pKa = 3.44MAKK243 pKa = 9.62IVVYY247 pKa = 10.86QRR249 pKa = 11.84MGWVGFSAGG258 pKa = 3.51

Molecular weight:
29.77 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

228

0

228

43957

27

750

192.8

22.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.584 ± 0.149

1.253 ± 0.062

5.276 ± 0.125

7.421 ± 0.202

4.655 ± 0.139

6.406 ± 0.156

2.248 ± 0.077

6.424 ± 0.126

6.795 ± 0.185

10.005 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.085

4.084 ± 0.118

4.177 ± 0.11

4.259 ± 0.113

6.242 ± 0.152

6.142 ± 0.13

4.411 ± 0.09

5.892 ± 0.129

1.638 ± 0.089

3.638 ± 0.101

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski