Aphis glycines virus 2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S1MNC2|A0A0S1MNC2_9VIRU Putative readthrough protein OS=Aphis glycines virus 2 OX=1897732 PE=3 SV=1
MM1 pKa = 7.88DD2 pKa = 5.5CSNPVNVNDD11 pKa = 5.4RR12 pKa = 11.84ITVGDD17 pKa = 3.87ARR19 pKa = 11.84IAAAGYY25 pKa = 9.48IRR27 pKa = 11.84QDD29 pKa = 3.33LASLKK34 pKa = 10.18NAAAKK39 pKa = 10.36VPRR42 pKa = 11.84NYY44 pKa = 10.69LPTSTPRR51 pKa = 11.84PPDD54 pKa = 3.32VVRR57 pKa = 11.84ADD59 pKa = 3.8LLRR62 pKa = 11.84VKK64 pKa = 9.69TSRR67 pKa = 11.84RR68 pKa = 11.84TVPNDD73 pKa = 3.33PEE75 pKa = 4.24GLARR79 pKa = 11.84LNDD82 pKa = 3.6LSEE85 pKa = 4.2FLTLEE90 pKa = 4.26AEE92 pKa = 4.62SIPICGYY99 pKa = 10.08VGHH102 pKa = 6.59QGNVHH107 pKa = 6.18PSGVKK112 pKa = 8.7MKK114 pKa = 10.28QGRR117 pKa = 11.84SLATRR122 pKa = 11.84TFGGQTRR129 pKa = 11.84DD130 pKa = 4.11DD131 pKa = 3.87IVLEE135 pKa = 3.8AALRR139 pKa = 11.84HH140 pKa = 5.49FPYY143 pKa = 9.76MDD145 pKa = 2.94VAAAYY150 pKa = 9.98SSAVYY155 pKa = 9.76TSGTPSGFLEE165 pKa = 4.08RR166 pKa = 11.84MRR168 pKa = 11.84LNMTRR173 pKa = 11.84PAVSSLRR180 pKa = 11.84TANVRR185 pKa = 11.84NIPQLAALMEE195 pKa = 4.49RR196 pKa = 11.84LLPIRR201 pKa = 11.84KK202 pKa = 9.45APVPDD207 pKa = 3.47WNVDD211 pKa = 3.95FDD213 pKa = 4.15VLLDD217 pKa = 3.76DD218 pKa = 5.15VEE220 pKa = 4.37ITKK223 pKa = 10.29EE224 pKa = 3.71AGAGYY229 pKa = 8.61PYY231 pKa = 10.39CRR233 pKa = 11.84SKK235 pKa = 10.48GQAMRR240 pKa = 11.84DD241 pKa = 3.71CLEE244 pKa = 3.94VVLPNIVEE252 pKa = 4.23AVTTGTWEE260 pKa = 3.86QLMVDD265 pKa = 3.73QPEE268 pKa = 4.25LFLVEE273 pKa = 4.39VKK275 pKa = 10.67NKK277 pKa = 9.84QDD279 pKa = 3.19TYY281 pKa = 11.49DD282 pKa = 3.53VEE284 pKa = 4.85KK285 pKa = 11.01LMTKK289 pKa = 8.11TRR291 pKa = 11.84PYY293 pKa = 9.86MNYY296 pKa = 9.46PMHH299 pKa = 6.6FTLFWSFMAQHH310 pKa = 6.08YY311 pKa = 10.0NEE313 pKa = 4.77LMYY316 pKa = 10.93KK317 pKa = 10.3VGDD320 pKa = 3.98GPATSNACGWSMAAGGGKK338 pKa = 9.85RR339 pKa = 11.84KK340 pKa = 9.49IEE342 pKa = 4.23YY343 pKa = 9.9INKK346 pKa = 7.61QLKK349 pKa = 10.18RR350 pKa = 11.84EE351 pKa = 4.02GDD353 pKa = 3.77WFVEE357 pKa = 4.78AYY359 pKa = 10.47SDD361 pKa = 5.09DD362 pKa = 3.45VVIYY366 pKa = 8.27YY367 pKa = 9.26KK368 pKa = 10.3QHH370 pKa = 5.96GEE372 pKa = 4.15VYY374 pKa = 10.13RR375 pKa = 11.84ISPDD379 pKa = 3.43FVQMDD384 pKa = 3.55SSIDD388 pKa = 3.5ADD390 pKa = 3.36IVRR393 pKa = 11.84ARR395 pKa = 11.84LYY397 pKa = 10.55NIKK400 pKa = 10.42KK401 pKa = 9.92LFKK404 pKa = 9.45EE405 pKa = 3.82QHH407 pKa = 6.17GDD409 pKa = 3.85NVWMEE414 pKa = 4.03SMIDD418 pKa = 3.37LWEE421 pKa = 4.72DD422 pKa = 3.22MALDD426 pKa = 3.79PMLIVQGTKK435 pKa = 7.92IWRR438 pKa = 11.84KK439 pKa = 7.7KK440 pKa = 9.75AKK442 pKa = 9.83HH443 pKa = 5.47GMCSGVPDD451 pKa = 3.52TTGINTYY458 pKa = 10.58KK459 pKa = 10.67SVLAYY464 pKa = 10.49HH465 pKa = 6.87KK466 pKa = 9.45MLEE469 pKa = 4.27SHH471 pKa = 7.63DD472 pKa = 4.04PRR474 pKa = 11.84TLTEE478 pKa = 3.73EE479 pKa = 4.5VARR482 pKa = 11.84KK483 pKa = 7.73WMRR486 pKa = 11.84VHH488 pKa = 7.08AGLEE492 pKa = 4.18IKK494 pKa = 10.46EE495 pKa = 4.48GTWTVEE501 pKa = 4.06PANFMAPPDD510 pKa = 4.06EE511 pKa = 4.54PMCPQTFLGVQWMNTLEE528 pKa = 4.36GTVVPYY534 pKa = 10.99LPTEE538 pKa = 3.34KK539 pKa = 9.47WLRR542 pKa = 11.84MLVVPKK548 pKa = 10.33DD549 pKa = 3.69KK550 pKa = 11.31EE551 pKa = 3.85MDD553 pKa = 3.87SYY555 pKa = 11.96SLAAARR561 pKa = 11.84RR562 pKa = 11.84RR563 pKa = 11.84FDD565 pKa = 3.19RR566 pKa = 11.84MRR568 pKa = 11.84GYY570 pKa = 10.81LITGAAFDD578 pKa = 3.69QRR580 pKa = 11.84IVNVINDD587 pKa = 4.1VLNGITPEE595 pKa = 3.81AVIMQVQAGNGKK607 pKa = 10.16GEE609 pKa = 4.02APQEE613 pKa = 4.06GLLLPDD619 pKa = 3.5YY620 pKa = 10.28VYY622 pKa = 10.32PASDD626 pKa = 3.62VVPTRR631 pKa = 11.84QDD633 pKa = 2.95VEE635 pKa = 4.42DD636 pKa = 4.08IYY638 pKa = 11.19RR639 pKa = 11.84AGAVRR644 pKa = 11.84TPWQEE649 pKa = 3.52LFPGVKK655 pKa = 9.77EE656 pKa = 4.45LIAGHH661 pKa = 6.37MDD663 pKa = 3.88LNQRR667 pKa = 11.84KK668 pKa = 8.47FGKK671 pKa = 9.78IKK673 pKa = 10.19PYY675 pKa = 9.46RR676 pKa = 11.84AKK678 pKa = 10.73LAGAGEE684 pKa = 4.48GKK686 pKa = 10.81YY687 pKa = 10.52SDD689 pKa = 4.74FMVLTPPEE697 pKa = 4.35EE698 pKa = 4.34FASPAQNPGISGAPYY713 pKa = 10.51KK714 pKa = 10.28KK715 pKa = 10.42GKK717 pKa = 7.45HH718 pKa = 4.7TKK720 pKa = 9.68PGNKK724 pKa = 9.4CNVVLPDD731 pKa = 3.62GEE733 pKa = 4.46RR734 pKa = 11.84GKK736 pKa = 9.18TMPTVEE742 pKa = 4.91DD743 pKa = 4.47VIDD746 pKa = 3.66HH747 pKa = 7.18ALRR750 pKa = 11.84EE751 pKa = 4.4SAMPLSLLAQKK762 pKa = 10.27LGKK765 pKa = 9.69EE766 pKa = 3.88PHH768 pKa = 5.8EE769 pKa = 4.35TTKK772 pKa = 10.42FVYY775 pKa = 9.97NAGYY779 pKa = 9.68DD780 pKa = 3.37ARR782 pKa = 11.84EE783 pKa = 3.95PLLGVKK789 pKa = 9.78SEE791 pKa = 4.23NLGPALHH798 pKa = 6.67SLFPVKK804 pKa = 10.02EE805 pKa = 3.96RR806 pKa = 11.84LRR808 pKa = 11.84VRR810 pKa = 11.84EE811 pKa = 4.17TKK813 pKa = 10.44MMDD816 pKa = 3.43DD817 pKa = 3.84GKK819 pKa = 8.21TTVAITKK826 pKa = 9.19PDD828 pKa = 3.35TRR830 pKa = 11.84VTPLRR835 pKa = 11.84GTVVHH840 pKa = 7.37DD841 pKa = 4.04EE842 pKa = 4.48FNGEE846 pKa = 3.67ARR848 pKa = 11.84SPPRR852 pKa = 11.84EE853 pKa = 4.1VKK855 pKa = 10.44VIHH858 pKa = 6.63HH859 pKa = 6.65PLVTSSALVGDD870 pKa = 3.98PVSYY874 pKa = 9.74MVHH877 pKa = 7.28LLTKK881 pKa = 9.47ATGEE885 pKa = 3.92NGEE888 pKa = 4.24WSTTTVPTTIEE899 pKa = 3.83AGTAVVFRR907 pKa = 11.84AEE909 pKa = 3.91YY910 pKa = 9.54SHH912 pKa = 7.87AGFTTHH918 pKa = 6.94AVANSIKK925 pKa = 9.99KK926 pKa = 10.15AKK928 pKa = 10.17AFASRR933 pKa = 11.84VMIDD937 pKa = 3.35KK938 pKa = 10.62LIRR941 pKa = 11.84DD942 pKa = 4.4HH943 pKa = 6.51GVPMPSAYY951 pKa = 9.53KK952 pKa = 10.18PPTPSEE958 pKa = 4.23VIEE961 pKa = 4.12VSARR965 pKa = 11.84ARR967 pKa = 11.84AEE969 pKa = 3.73SLQAHH974 pKa = 6.44NNPSWKK980 pKa = 8.41TAKK983 pKa = 10.4NNQAKK988 pKa = 10.27LDD990 pKa = 3.86YY991 pKa = 9.79AQQKK995 pKa = 8.9SAQIDD1000 pKa = 3.49EE1001 pKa = 5.15RR1002 pKa = 11.84IATRR1006 pKa = 11.84LGPDD1010 pKa = 3.08KK1011 pKa = 10.85PLDD1014 pKa = 3.68EE1015 pKa = 4.89LLVDD1019 pKa = 4.27RR1020 pKa = 11.84FEE1022 pKa = 4.98KK1023 pKa = 10.79LATALTDD1030 pKa = 3.76RR1031 pKa = 11.84LNDD1034 pKa = 4.66AIKK1037 pKa = 10.38QSLGISLEE1045 pKa = 4.03VEE1047 pKa = 3.97ALDD1050 pKa = 4.51SRR1052 pKa = 11.84KK1053 pKa = 9.85KK1054 pKa = 10.91SKK1056 pKa = 9.51TKK1058 pKa = 10.73NKK1060 pKa = 8.9STSDD1064 pKa = 3.2KK1065 pKa = 10.5KK1066 pKa = 11.0AKK1068 pKa = 8.45KK1069 pKa = 8.46TNHH1072 pKa = 5.88KK1073 pKa = 9.07TNSDD1077 pKa = 3.46YY1078 pKa = 10.84DD1079 pKa = 3.74TTNQEE1084 pKa = 3.43EE1085 pKa = 4.24SQYY1088 pKa = 11.15YY1089 pKa = 9.03EE1090 pKa = 4.63DD1091 pKa = 4.24YY1092 pKa = 11.8GEE1094 pKa = 4.55GTSNRR1099 pKa = 11.84YY1100 pKa = 7.65GAPYY1104 pKa = 10.19DD1105 pKa = 3.92EE1106 pKa = 5.42EE1107 pKa = 4.42EE1108 pKa = 4.79GPSKK1112 pKa = 10.76KK1113 pKa = 10.45GMRR1116 pKa = 11.84SRR1118 pKa = 11.84RR1119 pKa = 11.84RR1120 pKa = 11.84RR1121 pKa = 3.32

Molecular weight: 125.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S1MNB6|A0A0S1MNB6_9VIRU Replicase OS=Aphis glycines virus 2 OX=1897732 PE=4 SV=1
MM1 pKa = 7.19TRR3 pKa = 11.84RR4 pKa = 11.84TKK6 pKa = 10.51KK7 pKa = 10.08RR8 pKa = 11.84ANTTKK13 pKa = 10.14TMEE16 pKa = 4.41KK17 pKa = 10.55ALVTAMEE24 pKa = 4.33RR25 pKa = 11.84LTTKK29 pKa = 10.32RR30 pKa = 11.84RR31 pKa = 11.84VPRR34 pKa = 11.84KK35 pKa = 9.41KK36 pKa = 9.19GCARR40 pKa = 11.84VAEE43 pKa = 4.52GEE45 pKa = 4.14MVLRR49 pKa = 11.84RR50 pKa = 11.84EE51 pKa = 4.02EE52 pKa = 3.99MLVDD56 pKa = 3.57VTLSANKK63 pKa = 9.04TDD65 pKa = 3.66STGSVVLALANFPWLKK81 pKa = 9.38TVAGSFEE88 pKa = 4.1RR89 pKa = 11.84YY90 pKa = 7.3KK91 pKa = 10.22WKK93 pKa = 10.28RR94 pKa = 11.84LNIHH98 pKa = 5.54WRR100 pKa = 11.84AAGGFNKK107 pKa = 10.35GGLIAVGMDD116 pKa = 3.27WSNQLSSAYY125 pKa = 8.88TRR127 pKa = 11.84QTLTSASEE135 pKa = 4.08RR136 pKa = 11.84QKK138 pKa = 11.18VLSLTPHH145 pKa = 6.0MSLPISSTSINKK157 pKa = 8.45TLGLPIKK164 pKa = 9.62MLNSRR169 pKa = 11.84NWYY172 pKa = 9.54DD173 pKa = 3.23AAKK176 pKa = 9.48TDD178 pKa = 4.31DD179 pKa = 4.05EE180 pKa = 4.88GAVGAIRR187 pKa = 11.84YY188 pKa = 7.57SAKK191 pKa = 10.15CDD193 pKa = 3.26SDD195 pKa = 3.7TVEE198 pKa = 4.39RR199 pKa = 11.84FIGEE203 pKa = 3.48IWVDD207 pKa = 3.62YY208 pKa = 10.72EE209 pKa = 4.51VVLQGTRR216 pKa = 11.84AA217 pKa = 3.18

Molecular weight: 24.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1823

217

1121

607.7

68.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.283 ± 0.337

0.768 ± 0.056

5.595 ± 0.634

5.979 ± 0.504

2.743 ± 0.447

6.253 ± 0.273

1.865 ± 0.366

3.73 ± 0.102

6.802 ± 0.398

8.338 ± 0.635

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.236 ± 0.291

4.114 ± 0.011

5.705 ± 0.967

2.907 ± 0.253

6.967 ± 0.648

6.308 ± 0.826

7.625 ± 0.986

7.57 ± 0.18

1.591 ± 0.32

3.566 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski