Lachnospiraceae bacterium OF09-6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A373A895|A0A373A895_9FIRM ABC transporter permease OS=Lachnospiraceae bacterium OF09-6 OX=2293831 GN=DXA98_14905 PE=3 SV=1
MM1 pKa = 7.08MKK3 pKa = 10.56KK4 pKa = 9.02LTVLAAAAMLAALPATGVSAAGDD27 pKa = 3.54LTFATGGSSGTYY39 pKa = 8.5YY40 pKa = 11.37AFGSVLAGEE49 pKa = 4.67VSSDD53 pKa = 3.02TDD55 pKa = 3.56TKK57 pKa = 9.73VTAVEE62 pKa = 4.64SNGSADD68 pKa = 4.62NVDD71 pKa = 3.59QVDD74 pKa = 4.39AGDD77 pKa = 4.18ADD79 pKa = 3.61IAFCQSDD86 pKa = 3.93VMAYY90 pKa = 10.2AHH92 pKa = 7.16DD93 pKa = 5.0GINLFEE99 pKa = 5.27GDD101 pKa = 4.74PIDD104 pKa = 4.48SFSTVAALYY113 pKa = 9.91QEE115 pKa = 4.28QVQIITTNPDD125 pKa = 2.64IKK127 pKa = 10.06TVADD131 pKa = 3.87LKK133 pKa = 10.95GKK135 pKa = 9.65NVSIGASGSGVYY147 pKa = 9.97FNAIDD152 pKa = 4.07LLNAYY157 pKa = 9.54GLTEE161 pKa = 3.98EE162 pKa = 5.68DD163 pKa = 3.39INPTYY168 pKa = 10.88EE169 pKa = 4.07SFADD173 pKa = 3.73SADD176 pKa = 3.51SLKK179 pKa = 10.95DD180 pKa = 3.49GKK182 pKa = 10.36IDD184 pKa = 3.6AAFITSGAPTTAVTDD199 pKa = 4.14LGTAKK204 pKa = 10.32DD205 pKa = 3.94VYY207 pKa = 10.63LVSIDD212 pKa = 4.02DD213 pKa = 3.79EE214 pKa = 4.54HH215 pKa = 6.67MKK217 pKa = 9.76TLLEE221 pKa = 4.07EE222 pKa = 4.25CPYY225 pKa = 9.28YY226 pKa = 10.66TEE228 pKa = 4.27NTISADD234 pKa = 3.49TYY236 pKa = 11.74GLDD239 pKa = 3.58EE240 pKa = 4.69DD241 pKa = 4.6VKK243 pKa = 9.03TVAVGAVVIASNDD256 pKa = 3.35VAEE259 pKa = 4.43DD260 pKa = 4.29DD261 pKa = 3.9IYY263 pKa = 11.75NFVSAVFEE271 pKa = 4.08NTDD274 pKa = 3.85AIEE277 pKa = 4.01TAHH280 pKa = 6.97AKK282 pKa = 9.97GAEE285 pKa = 3.98LDD287 pKa = 3.53LDD289 pKa = 3.98YY290 pKa = 11.03AASITSVPYY299 pKa = 9.78HH300 pKa = 6.72AGAAKK305 pKa = 10.09YY306 pKa = 10.15FEE308 pKa = 4.68EE309 pKa = 5.18KK310 pKa = 10.72GLTVEE315 pKa = 4.72TEE317 pKa = 3.79

Molecular weight: 33.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A373A7N1|A0A373A7N1_9FIRM YbaK/EbsC family protein OS=Lachnospiraceae bacterium OF09-6 OX=2293831 GN=DXA98_12505 PE=4 SV=1
MM1 pKa = 7.7AKK3 pKa = 8.05MTFQPKK9 pKa = 9.15KK10 pKa = 7.58RR11 pKa = 11.84QRR13 pKa = 11.84SKK15 pKa = 9.0VHH17 pKa = 5.93GFRR20 pKa = 11.84SRR22 pKa = 11.84MSTPGGRR29 pKa = 11.84KK30 pKa = 8.8VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.71GRR40 pKa = 11.84KK41 pKa = 8.73VLSAA45 pKa = 4.05

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2857

0

2857

872464

14

3784

305.4

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.417 ± 0.049

1.576 ± 0.022

5.558 ± 0.045

7.82 ± 0.052

3.863 ± 0.037

7.019 ± 0.044

1.824 ± 0.022

7.294 ± 0.043

6.748 ± 0.047

8.874 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.325 ± 0.028

4.186 ± 0.029

3.162 ± 0.023

3.668 ± 0.029

4.303 ± 0.039

5.726 ± 0.037

5.642 ± 0.057

6.806 ± 0.032

0.978 ± 0.015

4.212 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski