Streptomyces sp. SID7982

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B3GEV9|A0A6B3GEV9_9ACTN Peroxiredoxin (Fragment) OS=Streptomyces sp. SID7982 OX=2706094 GN=G3M53_48590 PE=4 SV=1
AA1 pKa = 7.8AEE3 pKa = 4.27AAPAPVAAPAAVPAHH18 pKa = 6.46AVTGYY23 pKa = 8.39WQNFNNGATVQTLADD38 pKa = 3.69VPDD41 pKa = 4.69AYY43 pKa = 10.84DD44 pKa = 4.0IIAVSFADD52 pKa = 3.56ATAEE56 pKa = 4.0PGEE59 pKa = 4.22ITFTLDD65 pKa = 2.87SAGLGGYY72 pKa = 7.74TDD74 pKa = 3.51EE75 pKa = 5.29QFRR78 pKa = 11.84ADD80 pKa = 4.05LAAKK84 pKa = 9.65QADD87 pKa = 4.03GKK89 pKa = 10.69SVIISVGGEE98 pKa = 3.7KK99 pKa = 10.64GAVAVNDD106 pKa = 3.77SASAQRR112 pKa = 11.84FADD115 pKa = 3.25STYY118 pKa = 11.42ALMRR122 pKa = 11.84EE123 pKa = 4.13YY124 pKa = 11.03GFDD127 pKa = 3.53GVDD130 pKa = 2.97IDD132 pKa = 6.01LEE134 pKa = 4.43NGLNSTYY141 pKa = 8.08MTEE144 pKa = 4.38ALTKK148 pKa = 9.78LHH150 pKa = 6.64EE151 pKa = 4.43KK152 pKa = 10.76AGDD155 pKa = 3.85GLVLTMAPQTIDD167 pKa = 3.19MQSPEE172 pKa = 3.83NEE174 pKa = 4.08YY175 pKa = 10.8FKK177 pKa = 10.61TALAVKK183 pKa = 10.53DD184 pKa = 3.81FLTVVNMQYY193 pKa = 11.07YY194 pKa = 10.53NSGSMLGCDD203 pKa = 3.33GQVYY207 pKa = 10.18SQGTVDD213 pKa = 5.79FLTALACIQLEE224 pKa = 4.2NGLDD228 pKa = 3.52ASQVGIGVPASPKK241 pKa = 9.95AAGGGYY247 pKa = 9.27VEE249 pKa = 4.59PSVVNDD255 pKa = 4.47ALDD258 pKa = 3.85CLTRR262 pKa = 11.84GTDD265 pKa = 3.36CGSFTPEE272 pKa = 3.24KK273 pKa = 9.66TYY275 pKa = 10.25PALRR279 pKa = 11.84GAMTWSTNWDD289 pKa = 3.68ADD291 pKa = 4.35NGNAWSNAVGPHH303 pKa = 6.36VDD305 pKa = 4.25DD306 pKa = 5.51LPP308 pKa = 5.91

Molecular weight: 32.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B3GR04|A0A6B3GR04_9ACTN Bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase OS=Streptomyces sp. SID7982 OX=2706094 GN=G3M53_85820 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19102

0

19102

3686652

20

5266

193.0

20.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.597 ± 0.027

0.76 ± 0.006

6.026 ± 0.018

5.998 ± 0.02

2.749 ± 0.013

9.557 ± 0.019

2.289 ± 0.011

3.077 ± 0.015

2.159 ± 0.017

10.484 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.707 ± 0.009

1.699 ± 0.01

6.096 ± 0.017

2.694 ± 0.012

8.027 ± 0.021

4.976 ± 0.016

6.115 ± 0.016

8.445 ± 0.02

1.5 ± 0.01

2.043 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski