Amino acid dipepetide frequency for Flavobacteria bacterium (strain BBFL7)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.294AlaAla: 5.294 ± 0.1	0.572AlaCys: 0.572 ± 0.038	3.931AlaAsp: 3.931 ± 0.119	3.128AlaGlu: 3.128 ± 0.072	3.332AlaPhe: 3.332 ± 0.064	5.003AlaGly: 5.003 ± 0.085	1.295AlaHis: 1.295 ± 0.04	5.473AlaIle: 5.473 ± 0.085	3.703AlaLys: 3.703 ± 0.077	6.103AlaLeu: 6.103 ± 0.093	1.7AlaMet: 1.7 ± 0.04	3.246AlaAsn: 3.246 ± 0.063	2.238AlaPro: 2.238 ± 0.062	3.368AlaGln: 3.368 ± 0.065	2.371AlaArg: 2.371 ± 0.054	4.466AlaSer: 4.466 ± 0.078	4.565AlaThr: 4.565 ± 0.095	4.651AlaVal: 4.651 ± 0.076	0.634AlaTrp: 0.634 ± 0.032	2.464AlaTyr: 2.464 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.466CysAla: 0.466 ± 0.023	0.084CysCys: 0.084 ± 0.011	0.614CysAsp: 0.614 ± 0.094	0.436CysGlu: 0.436 ± 0.024	0.336CysPhe: 0.336 ± 0.02	0.62CysGly: 0.62 ± 0.03	0.191CysHis: 0.191 ± 0.013	0.544CysIle: 0.544 ± 0.026	0.433CysLys: 0.433 ± 0.022	0.605CysLeu: 0.605 ± 0.027	0.13CysMet: 0.13 ± 0.012	0.412CysAsn: 0.412 ± 0.024	0.319CysPro: 0.319 ± 0.022	0.202CysGln: 0.202 ± 0.014	0.187CysArg: 0.187 ± 0.014	0.543CysSer: 0.543 ± 0.046	0.416CysThr: 0.416 ± 0.023	0.478CysVal: 0.478 ± 0.022	0.086CysTrp: 0.086 ± 0.014	0.354CysTyr: 0.354 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	4.413AspAla: 4.413 ± 0.098	0.583AspCys: 0.583 ± 0.075	3.704AspAsp: 3.704 ± 0.083	3.88AspGlu: 3.88 ± 0.077	3.661AspPhe: 3.661 ± 0.068	4.654AspGly: 4.654 ± 0.207	1.212AspHis: 1.212 ± 0.038	4.673AspIle: 4.673 ± 0.071	3.586AspLys: 3.586 ± 0.069	5.931AspLeu: 5.931 ± 0.09	1.468AspMet: 1.468 ± 0.048	3.566AspAsn: 3.566 ± 0.076	2.153AspPro: 2.153 ± 0.138	2.406AspGln: 2.406 ± 0.048	2.35AspArg: 2.35 ± 0.057	3.723AspSer: 3.723 ± 0.071	3.157AspThr: 3.157 ± 0.077	4.202AspVal: 4.202 ± 0.08	0.749AspTrp: 0.749 ± 0.032	3.213AspTyr: 3.213 ± 0.065	0.0AspXaa: 0.0 ± 0.0
Glu	3.866GluAla: 3.866 ± 0.076	0.346GluCys: 0.346 ± 0.02	3.653GluAsp: 3.653 ± 0.066	4.589GluGlu: 4.589 ± 0.102	2.77GluPhe: 2.77 ± 0.059	3.003GluGly: 3.003 ± 0.058	1.181GluHis: 1.181 ± 0.043	5.177GluIle: 5.177 ± 0.077	4.831GluLys: 4.831 ± 0.097	6.324GluLeu: 6.324 ± 0.093	1.63GluMet: 1.63 ± 0.047	4.257GluAsn: 4.257 ± 0.077	1.67GluPro: 1.67 ± 0.047	2.491GluGln: 2.491 ± 0.058	2.517GluArg: 2.517 ± 0.061	3.537GluSer: 3.537 ± 0.061	3.336GluThr: 3.336 ± 0.078	4.178GluVal: 4.178 ± 0.071	0.659GluTrp: 0.659 ± 0.028	2.338GluTyr: 2.338 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	3.008PheAla: 3.008 ± 0.065	0.363PheCys: 0.363 ± 0.023	3.118PheAsp: 3.118 ± 0.061	3.097PheGlu: 3.097 ± 0.064	2.38PhePhe: 2.38 ± 0.065	2.831PheGly: 2.831 ± 0.061	0.852PheHis: 0.852 ± 0.031	3.837PheIle: 3.837 ± 0.079	3.621PheLys: 3.621 ± 0.081	4.251PheLeu: 4.251 ± 0.078	1.133PheMet: 1.133 ± 0.035	3.393PheAsn: 3.393 ± 0.064	1.582PhePro: 1.582 ± 0.04	1.498PheGln: 1.498 ± 0.037	1.46PheArg: 1.46 ± 0.045	3.369PheSer: 3.369 ± 0.069	3.404PheThr: 3.404 ± 0.085	2.578PheVal: 2.578 ± 0.058	0.496PheTrp: 0.496 ± 0.025	2.158PheTyr: 2.158 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	4.393GlyAla: 4.393 ± 0.076	0.571GlyCys: 0.571 ± 0.026	4.131GlyAsp: 4.131 ± 0.125	3.319GlyGlu: 3.319 ± 0.063	3.385GlyPhe: 3.385 ± 0.062	4.388GlyGly: 4.388 ± 0.091	1.23GlyHis: 1.23 ± 0.04	5.345GlyIle: 5.345 ± 0.095	4.253GlyLys: 4.253 ± 0.075	5.782GlyLeu: 5.782 ± 0.085	1.753GlyMet: 1.753 ± 0.051	3.773GlyAsn: 3.773 ± 0.087	1.529GlyPro: 1.529 ± 0.055	2.019GlyGln: 2.019 ± 0.046	2.233GlyArg: 2.233 ± 0.052	4.259GlySer: 4.259 ± 0.077	4.571GlyThr: 4.571 ± 0.119	4.413GlyVal: 4.413 ± 0.086	0.754GlyTrp: 0.754 ± 0.037	2.847GlyTyr: 2.847 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	1.097HisAla: 1.097 ± 0.035	0.184HisCys: 0.184 ± 0.015	1.082HisAsp: 1.082 ± 0.035	0.976HisGlu: 0.976 ± 0.032	1.108HisPhe: 1.108 ± 0.037	1.158HisGly: 1.158 ± 0.035	0.574HisHis: 0.574 ± 0.027	1.499HisIle: 1.499 ± 0.042	1.294HisLys: 1.294 ± 0.042	1.903HisLeu: 1.903 ± 0.056	0.403HisMet: 0.403 ± 0.022	0.985HisAsn: 0.985 ± 0.033	0.812HisPro: 0.812 ± 0.035	0.772HisGln: 0.772 ± 0.032	0.757HisArg: 0.757 ± 0.033	1.103HisSer: 1.103 ± 0.037	0.969HisThr: 0.969 ± 0.029	1.083HisVal: 1.083 ± 0.038	0.214HisTrp: 0.214 ± 0.015	0.822HisTyr: 0.822 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.123IleAla: 6.123 ± 0.096	0.586IleCys: 0.586 ± 0.025	5.49IleAsp: 5.49 ± 0.073	5.485IleGlu: 5.485 ± 0.084	3.211IlePhe: 3.211 ± 0.078	4.983IleGly: 4.983 ± 0.088	1.385IleHis: 1.385 ± 0.042	6.053IleIle: 6.053 ± 0.1	5.245IleLys: 5.245 ± 0.089	6.684IleLeu: 6.684 ± 0.102	1.527IleMet: 1.527 ± 0.048	4.751IleAsn: 4.751 ± 0.076	3.134IlePro: 3.134 ± 0.057	2.466IleGln: 2.466 ± 0.052	2.179IleArg: 2.179 ± 0.046	5.338IleSer: 5.338 ± 0.087	5.145IleThr: 5.145 ± 0.082	4.556IleVal: 4.556 ± 0.069	0.642IleTrp: 0.642 ± 0.032	2.858IleTyr: 2.858 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.727LysAla: 4.727 ± 0.084	0.305LysCys: 0.305 ± 0.018	4.17LysAsp: 4.17 ± 0.076	5.175LysGlu: 5.175 ± 0.1	2.388LysPhe: 2.388 ± 0.048	3.743LysGly: 3.743 ± 0.084	1.197LysHis: 1.197 ± 0.04	4.711LysIle: 4.711 ± 0.078	5.478LysLys: 5.478 ± 0.096	5.571LysLeu: 5.571 ± 0.089	1.838LysMet: 1.838 ± 0.057	4.2LysAsn: 4.2 ± 0.089	2.038LysPro: 2.038 ± 0.055	2.226LysGln: 2.226 ± 0.047	2.696LysArg: 2.696 ± 0.066	4.097LysSer: 4.097 ± 0.069	3.881LysThr: 3.881 ± 0.085	3.94LysVal: 3.94 ± 0.076	0.748LysTrp: 0.748 ± 0.028	2.482LysTyr: 2.482 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	5.518LeuAla: 5.518 ± 0.087	0.67LeuCys: 0.67 ± 0.029	5.563LeuAsp: 5.563 ± 0.083	6.106LeuGlu: 6.106 ± 0.095	4.472LeuPhe: 4.472 ± 0.08	5.703LeuGly: 5.703 ± 0.082	1.599LeuHis: 1.599 ± 0.046	7.088LeuIle: 7.088 ± 0.099	6.773LeuLys: 6.773 ± 0.107	8.5LeuLeu: 8.5 ± 0.127	2.122LeuMet: 2.122 ± 0.054	5.659LeuAsn: 5.659 ± 0.095	3.404LeuPro: 3.404 ± 0.068	3.341LeuGln: 3.341 ± 0.07	3.361LeuArg: 3.361 ± 0.069	6.633LeuSer: 6.633 ± 0.084	5.201LeuThr: 5.201 ± 0.082	5.21LeuVal: 5.21 ± 0.08	0.863LeuTrp: 0.863 ± 0.033	3.136LeuTyr: 3.136 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	1.638MetAla: 1.638 ± 0.047	0.156MetCys: 0.156 ± 0.014	1.444MetAsp: 1.444 ± 0.042	1.463MetGlu: 1.463 ± 0.046	0.788MetPhe: 0.788 ± 0.027	1.491MetGly: 1.491 ± 0.046	0.412MetHis: 0.412 ± 0.024	1.838MetIle: 1.838 ± 0.044	2.107MetLys: 2.107 ± 0.051	2.058MetLeu: 2.058 ± 0.047	0.771MetMet: 0.771 ± 0.029	1.404MetAsn: 1.404 ± 0.036	0.906MetPro: 0.906 ± 0.031	0.835MetGln: 0.835 ± 0.031	1.037MetArg: 1.037 ± 0.034	1.627MetSer: 1.627 ± 0.044	1.402MetThr: 1.402 ± 0.037	1.454MetVal: 1.454 ± 0.043	0.159MetTrp: 0.159 ± 0.013	0.726MetTyr: 0.726 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	4.045AsnAla: 4.045 ± 0.101	0.468AsnCys: 0.468 ± 0.026	3.84AsnAsp: 3.84 ± 0.085	3.447AsnGlu: 3.447 ± 0.063	2.684AsnPhe: 2.684 ± 0.06	4.572AsnGly: 4.572 ± 0.106	1.197AsnHis: 1.197 ± 0.034	4.212AsnIle: 4.212 ± 0.076	3.494AsnLys: 3.494 ± 0.074	5.049AsnLeu: 5.049 ± 0.093	1.29AsnMet: 1.29 ± 0.033	3.753AsnAsn: 3.753 ± 0.091	2.773AsnPro: 2.773 ± 0.07	2.28AsnGln: 2.28 ± 0.055	2.293AsnArg: 2.293 ± 0.05	3.854AsnSer: 3.854 ± 0.085	3.565AsnThr: 3.565 ± 0.082	3.542AsnVal: 3.542 ± 0.07	0.742AsnTrp: 0.742 ± 0.032	2.778AsnTyr: 2.778 ± 0.068	0.0AsnXaa: 0.0 ± 0.0
Pro	2.412ProAla: 2.412 ± 0.068	0.266ProCys: 0.266 ± 0.044	2.454ProAsp: 2.454 ± 0.07	2.496ProGlu: 2.496 ± 0.054	1.737ProPhe: 1.737 ± 0.041	2.104ProGly: 2.104 ± 0.049	0.644ProHis: 0.644 ± 0.027	2.62ProIle: 2.62 ± 0.055	1.795ProLys: 1.795 ± 0.05	2.904ProLeu: 2.904 ± 0.052	0.758ProMet: 0.758 ± 0.025	1.979ProAsn: 1.979 ± 0.067	0.773ProPro: 0.773 ± 0.033	1.461ProGln: 1.461 ± 0.04	1.008ProArg: 1.008 ± 0.037	2.3ProSer: 2.3 ± 0.053	2.084ProThr: 2.084 ± 0.053	2.721ProVal: 2.721 ± 0.061	0.334ProTrp: 0.334 ± 0.021	1.442ProTyr: 1.442 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	2.317GlnAla: 2.317 ± 0.052	0.191GlnCys: 0.191 ± 0.016	2.073GlnAsp: 2.073 ± 0.058	2.65GlnGlu: 2.65 ± 0.062	1.785GlnPhe: 1.785 ± 0.045	1.99GlnGly: 1.99 ± 0.048	0.73GlnHis: 0.73 ± 0.029	2.824GlnIle: 2.824 ± 0.055	2.295GlnLys: 2.295 ± 0.056	4.203GlnLeu: 4.203 ± 0.08	0.887GlnMet: 0.887 ± 0.034	2.0GlnAsn: 2.0 ± 0.047	1.337GlnPro: 1.337 ± 0.038	1.803GlnGln: 1.803 ± 0.05	1.478GlnArg: 1.478 ± 0.042	2.354GlnSer: 2.354 ± 0.05	2.05GlnThr: 2.05 ± 0.052	2.43GlnVal: 2.43 ± 0.051	0.463GlnTrp: 0.463 ± 0.025	1.467GlnTyr: 1.467 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.282ArgAla: 2.282 ± 0.055	0.196ArgCys: 0.196 ± 0.014	2.15ArgAsp: 2.15 ± 0.051	2.374ArgGlu: 2.374 ± 0.056	2.114ArgPhe: 2.114 ± 0.055	2.109ArgGly: 2.109 ± 0.051	0.641ArgHis: 0.641 ± 0.027	2.942ArgIle: 2.942 ± 0.061	2.753ArgLys: 2.753 ± 0.062	3.361ArgLeu: 3.361 ± 0.065	0.978ArgMet: 0.978 ± 0.033	2.183ArgAsn: 2.183 ± 0.059	1.145ArgPro: 1.145 ± 0.033	1.133ArgGln: 1.133 ± 0.038	1.497ArgArg: 1.497 ± 0.048	2.168ArgSer: 2.168 ± 0.051	1.863ArgThr: 1.863 ± 0.048	2.331ArgVal: 2.331 ± 0.057	0.365ArgTrp: 0.365 ± 0.022	1.651ArgTyr: 1.651 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	3.697SerAla: 3.697 ± 0.073	0.66SerCys: 0.66 ± 0.033	3.851SerAsp: 3.851 ± 0.079	3.318SerGlu: 3.318 ± 0.066	3.657SerPhe: 3.657 ± 0.061	4.747SerGly: 4.747 ± 0.072	1.245SerHis: 1.245 ± 0.037	5.501SerIle: 5.501 ± 0.086	3.998SerLys: 3.998 ± 0.076	6.043SerLeu: 6.043 ± 0.088	1.464SerMet: 1.464 ± 0.042	4.069SerAsn: 4.069 ± 0.083	2.105SerPro: 2.105 ± 0.056	2.658SerGln: 2.658 ± 0.057	2.449SerArg: 2.449 ± 0.056	4.624SerSer: 4.624 ± 0.094	4.134SerThr: 4.134 ± 0.09	3.892SerVal: 3.892 ± 0.081	0.727SerTrp: 0.727 ± 0.029	2.991SerTyr: 2.991 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	4.8ThrAla: 4.8 ± 0.107	0.349ThrCys: 0.349 ± 0.024	4.239ThrAsp: 4.239 ± 0.135	3.419ThrGlu: 3.419 ± 0.062	2.857ThrPhe: 2.857 ± 0.081	4.669ThrGly: 4.669 ± 0.095	0.986ThrHis: 0.986 ± 0.035	5.023ThrIle: 5.023 ± 0.083	2.838ThrLys: 2.838 ± 0.06	5.325ThrLeu: 5.325 ± 0.081	1.158ThrMet: 1.158 ± 0.031	3.265ThrAsn: 3.265 ± 0.108	2.549ThrPro: 2.549 ± 0.061	2.201ThrGln: 2.201 ± 0.049	1.896ThrArg: 1.896 ± 0.052	4.217ThrSer: 4.217 ± 0.105	4.235ThrThr: 4.235 ± 0.15	4.163ThrVal: 4.163 ± 0.088	0.55ThrTrp: 0.55 ± 0.028	2.485ThrTyr: 2.485 ± 0.062	0.0ThrXaa: 0.0 ± 0.0
Val	4.421ValAla: 4.421 ± 0.071	0.507ValCys: 0.507 ± 0.025	4.226ValAsp: 4.226 ± 0.079	4.01ValGlu: 4.01 ± 0.078	3.056ValPhe: 3.056 ± 0.067	3.731ValGly: 3.731 ± 0.073	1.045ValHis: 1.045 ± 0.032	5.041ValIle: 5.041 ± 0.076	3.807ValLys: 3.807 ± 0.083	5.605ValLeu: 5.605 ± 0.087	1.538ValMet: 1.538 ± 0.046	3.741ValAsn: 3.741 ± 0.076	2.241ValPro: 2.241 ± 0.051	2.107ValGln: 2.107 ± 0.053	2.179ValArg: 2.179 ± 0.045	4.257ValSer: 4.257 ± 0.089	4.271ValThr: 4.271 ± 0.122	4.285ValVal: 4.285 ± 0.076	0.55ValTrp: 0.55 ± 0.024	2.442ValTyr: 2.442 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.576TrpAla: 0.576 ± 0.026	0.097TrpCys: 0.097 ± 0.011	0.695TrpAsp: 0.695 ± 0.032	0.626TrpGlu: 0.626 ± 0.029	0.53TrpPhe: 0.53 ± 0.022	0.516TrpGly: 0.516 ± 0.027	0.225TrpHis: 0.225 ± 0.016	0.768TrpIle: 0.768 ± 0.026	0.678TrpLys: 0.678 ± 0.03	0.998TrpLeu: 0.998 ± 0.04	0.346TrpMet: 0.346 ± 0.02	0.769TrpAsn: 0.769 ± 0.033	0.239TrpPro: 0.239 ± 0.018	0.424TrpGln: 0.424 ± 0.024	0.437TrpArg: 0.437 ± 0.022	0.709TrpSer: 0.709 ± 0.029	0.561TrpThr: 0.561 ± 0.031	0.586TrpVal: 0.586 ± 0.026	0.182TrpTrp: 0.182 ± 0.015	0.45TrpTyr: 0.45 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.444TyrAla: 2.444 ± 0.061	0.305TyrCys: 0.305 ± 0.018	2.855TyrAsp: 2.855 ± 0.063	2.358TyrGlu: 2.358 ± 0.049	2.177TyrPhe: 2.177 ± 0.049	2.933TyrGly: 2.933 ± 0.06	0.953TyrHis: 0.953 ± 0.039	2.669TyrIle: 2.669 ± 0.057	2.63TyrLys: 2.63 ± 0.056	3.718TyrLeu: 3.718 ± 0.075	0.822TyrMet: 0.822 ± 0.033	2.475TyrAsn: 2.475 ± 0.056	1.347TyrPro: 1.347 ± 0.041	1.638TyrGln: 1.638 ± 0.042	1.849TyrArg: 1.849 ± 0.046	2.653TyrSer: 2.653 ± 0.063	2.416TyrThr: 2.416 ± 0.089	2.369TyrVal: 2.369 ± 0.053	0.492TyrTrp: 0.492 ± 0.024	1.751TyrTyr: 1.751 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2592 proteins (928669 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---