Kribbella sp. 16K104

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Kribbellaceae; Kribbella; unclassified Kribbella

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6875 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4WKC5|A0A4R4WKC5_9ACTN Uncharacterized protein OS=Kribbella sp. 16K104 OX=2530375 GN=E1218_26905 PE=4 SV=1
MM1 pKa = 7.5TNPAPGALAWFEE13 pKa = 4.39VATSDD18 pKa = 4.86PDD20 pKa = 3.88GAQKK24 pKa = 10.6FYY26 pKa = 11.48GSLFDD31 pKa = 3.51WTFEE35 pKa = 4.08ADD37 pKa = 3.55GPAASGGLDD46 pKa = 3.3YY47 pKa = 11.5RR48 pKa = 11.84NITASGAGAPMGGIAGTDD66 pKa = 3.5GQTPGHH72 pKa = 5.59SVFYY76 pKa = 10.63ILVADD81 pKa = 4.53VEE83 pKa = 4.67ATCADD88 pKa = 3.47ATQLGGSVVSKK99 pKa = 10.63HH100 pKa = 6.56LEE102 pKa = 4.04TAPGAPTFAYY112 pKa = 10.52LRR114 pKa = 11.84DD115 pKa = 3.44PAGNMFGVFTPPAAA129 pKa = 4.29

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4WSN2|A0A4R4WSN2_9ACTN Tyrosine--tRNA ligase OS=Kribbella sp. 16K104 OX=2530375 GN=tyrS PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.21GRR40 pKa = 11.84QRR42 pKa = 11.84LAAA45 pKa = 4.56

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6875

0

6875

2191178

28

3361

318.7

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.61 ± 0.039

0.726 ± 0.007

6.221 ± 0.024

5.434 ± 0.03

2.956 ± 0.019

9.036 ± 0.027

2.128 ± 0.014

3.793 ± 0.016

2.274 ± 0.023

10.338 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.744 ± 0.011

2.105 ± 0.018

5.738 ± 0.021

2.974 ± 0.017

7.513 ± 0.034

5.328 ± 0.023

6.233 ± 0.028

8.936 ± 0.026

1.633 ± 0.013

2.28 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski