Babesia bovis

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Piroplasmida; Babesiidae; Babesia

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7ATC9|A7ATC9_BABBO Prolyl endopeptidase OS=Babesia bovis OX=5865 GN=BBOV_II002340 PE=3 SV=1
MM1 pKa = 7.67AAPSEE6 pKa = 4.06CDD8 pKa = 3.37YY9 pKa = 11.38TSSEE13 pKa = 4.12DD14 pKa = 3.64SASDD18 pKa = 3.53VCSSSSCSCDD28 pKa = 3.14SGSCCSSEE36 pKa = 4.49SADD39 pKa = 3.56EE40 pKa = 4.9CSSCSCSPRR49 pKa = 11.84SAGSNDD55 pKa = 2.76EE56 pKa = 5.07HH57 pKa = 7.79YY58 pKa = 10.93QDD60 pKa = 6.25DD61 pKa = 4.55LDD63 pKa = 3.88QLKK66 pKa = 8.36TFIADD71 pKa = 3.32VASLIRR77 pKa = 11.84QSRR80 pKa = 11.84NTEE83 pKa = 4.03DD84 pKa = 3.73VPVTTNVEE92 pKa = 4.22VQVSDD97 pKa = 3.78EE98 pKa = 4.52KK99 pKa = 11.08PIEE102 pKa = 4.35TPVEE106 pKa = 4.06QPVAAPFNKK115 pKa = 10.47DD116 pKa = 3.14LDD118 pKa = 3.89NEE120 pKa = 4.28IANFEE125 pKa = 3.98QLINSTEE132 pKa = 3.79IKK134 pKa = 10.8LNGHH138 pKa = 5.89SGFCEE143 pKa = 4.58FEE145 pKa = 4.24AQEE148 pKa = 4.24SPYY151 pKa = 9.79ATTVADD157 pKa = 4.12TPVGYY162 pKa = 10.6CFTNDD167 pKa = 2.9DD168 pKa = 3.87TARR171 pKa = 11.84EE172 pKa = 4.0EE173 pKa = 4.16MPEE176 pKa = 4.08EE177 pKa = 4.34SCSEE181 pKa = 4.23SEE183 pKa = 5.7DD184 pKa = 3.7EE185 pKa = 6.04AEE187 pKa = 4.43PDD189 pKa = 3.5LDD191 pKa = 3.9KK192 pKa = 11.48LHH194 pKa = 6.61SRR196 pKa = 11.84LVALDD201 pKa = 3.17LHH203 pKa = 8.65SYY205 pKa = 8.51MGLVEE210 pKa = 4.54SVLDD214 pKa = 3.75KK215 pKa = 10.76RR216 pKa = 11.84VKK218 pKa = 10.7LDD220 pKa = 3.58LLL222 pKa = 4.12

Molecular weight: 24.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7AX42|A7AX42_BABBO Uncharacterized protein OS=Babesia bovis OX=5865 GN=BBOV_I000210 PE=4 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84SLAWLLFACLIGVATPFQAGNGRR25 pKa = 11.84QGAGNAPMLARR36 pKa = 11.84RR37 pKa = 11.84DD38 pKa = 3.76QALQAYY44 pKa = 8.28RR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 10.49NNSIFNRR54 pKa = 11.84ITEE57 pKa = 4.47SVNSIAEE64 pKa = 4.59SINQTVPGGIATAVTVPIVTVAAAVTIRR92 pKa = 11.84SLLAPAKK99 pKa = 9.73PKK101 pKa = 10.46AAAPALNRR109 pKa = 11.84YY110 pKa = 8.16QCTGCGFTIFPAKK123 pKa = 10.18NRR125 pKa = 11.84EE126 pKa = 3.68EE127 pKa = 4.49RR128 pKa = 11.84FFSSSFTCPNCGAAKK143 pKa = 9.75NKK145 pKa = 9.67FVEE148 pKa = 4.57RR149 pKa = 11.84HH150 pKa = 4.4

Molecular weight: 16.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3690

0

3690

1851840

38

4820

501.9

56.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.137 ± 0.032

2.234 ± 0.024

6.105 ± 0.027

6.08 ± 0.044

3.758 ± 0.024

5.786 ± 0.042

2.6 ± 0.015

6.082 ± 0.031

6.022 ± 0.041

9.464 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.017

4.841 ± 0.03

4.303 ± 0.026

3.5 ± 0.021

5.388 ± 0.033

8.087 ± 0.043

5.691 ± 0.027

6.642 ± 0.025

1.043 ± 0.012

3.621 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski