Bifidobacterium bifidum BGN4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium bifidum

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1834 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3WIW0|I3WIW0_BIFBI Putative DNA-binding protein OS=Bifidobacterium bifidum BGN4 OX=484020 GN=BBB_1231 PE=4 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.51YY5 pKa = 10.08DD6 pKa = 3.82SPRR9 pKa = 11.84NKK11 pKa = 10.84DD12 pKa = 3.11EE13 pKa = 5.41DD14 pKa = 3.99EE15 pKa = 4.81EE16 pKa = 4.49SLQEE20 pKa = 3.97LGKK23 pKa = 10.68GSRR26 pKa = 11.84SSSTDD31 pKa = 2.81MDD33 pKa = 4.69DD34 pKa = 4.76DD35 pKa = 4.31EE36 pKa = 4.81NAIAEE41 pKa = 4.35DD42 pKa = 4.23YY43 pKa = 9.11EE44 pKa = 5.05LPGADD49 pKa = 5.03LSNEE53 pKa = 4.1DD54 pKa = 3.77ASVTVIPMQNDD65 pKa = 2.99EE66 pKa = 5.41FICSEE71 pKa = 4.08CFLVKK76 pKa = 10.28HH77 pKa = 6.5RR78 pKa = 11.84SQLAYY83 pKa = 9.7VTDD86 pKa = 4.79DD87 pKa = 3.69GQPVCEE93 pKa = 4.19EE94 pKa = 4.24CAAA97 pKa = 3.98

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3WFH6|I3WFH6_BIFBI Putative metal-dependent hydrolase OS=Bifidobacterium bifidum BGN4 OX=484020 GN=BBB_0042 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84SMKK15 pKa = 9.49HH16 pKa = 4.07GFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AMINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.42GRR39 pKa = 11.84KK40 pKa = 8.88SLSAA44 pKa = 3.86

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1834

0

1834

627178

22

3133

342.0

37.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.144 ± 0.071

0.936 ± 0.02

6.816 ± 0.056

5.448 ± 0.057

3.267 ± 0.035

8.292 ± 0.057

2.154 ± 0.033

5.169 ± 0.038

3.994 ± 0.055

8.499 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.027

3.221 ± 0.04

4.71 ± 0.037

3.33 ± 0.038

6.244 ± 0.072

6.206 ± 0.049

6.169 ± 0.068

7.815 ± 0.054

1.305 ± 0.021

2.659 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski