Amino acid dipepetide frequency for Bifidobacterium bifidum BGN4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.396AlaAla: 14.396 ± 0.233
1.11AlaCys: 1.11 ± 0.043
7.841AlaAsp: 7.841 ± 0.128
5.872AlaGlu: 5.872 ± 0.104
3.307AlaPhe: 3.307 ± 0.068
9.618AlaGly: 9.618 ± 0.164
2.24AlaHis: 2.24 ± 0.059
5.41AlaIle: 5.41 ± 0.108
4.895AlaLys: 4.895 ± 0.108
9.911AlaLeu: 9.911 ± 0.145
3.141AlaMet: 3.141 ± 0.07
3.272AlaAsn: 3.272 ± 0.077
4.273AlaPro: 4.273 ± 0.118
4.208AlaGln: 4.208 ± 0.093
6.15AlaArg: 6.15 ± 0.136
6.768AlaSer: 6.768 ± 0.133
6.185AlaThr: 6.185 ± 0.118
8.714AlaVal: 8.714 ± 0.138
1.473AlaTrp: 1.473 ± 0.051
2.653AlaTyr: 2.653 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
1.105CysAla: 1.105 ± 0.046
0.14CysCys: 0.14 ± 0.018
0.627CysAsp: 0.627 ± 0.03
0.523CysGlu: 0.523 ± 0.026
0.314CysPhe: 0.314 ± 0.029
0.998CysGly: 0.998 ± 0.04
0.217CysHis: 0.217 ± 0.02
0.446CysIle: 0.446 ± 0.025
0.271CysLys: 0.271 ± 0.022
0.703CysLeu: 0.703 ± 0.043
0.244CysMet: 0.244 ± 0.023
0.242CysAsn: 0.242 ± 0.021
0.499CysPro: 0.499 ± 0.031
0.206CysGln: 0.206 ± 0.019
0.63CysArg: 0.63 ± 0.035
0.576CysSer: 0.576 ± 0.033
0.513CysThr: 0.513 ± 0.026
0.775CysVal: 0.775 ± 0.036
0.12CysTrp: 0.12 ± 0.015
0.207CysTyr: 0.207 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
7.709AspAla: 7.709 ± 0.129
0.534AspCys: 0.534 ± 0.026
5.321AspAsp: 5.321 ± 0.114
4.728AspGlu: 4.728 ± 0.097
2.192AspPhe: 2.192 ± 0.062
6.579AspGly: 6.579 ± 0.119
1.39AspHis: 1.39 ± 0.049
3.454AspIle: 3.454 ± 0.076
2.656AspLys: 2.656 ± 0.082
5.209AspLeu: 5.209 ± 0.103
1.81AspMet: 1.81 ± 0.054
1.807AspAsn: 1.807 ± 0.058
3.595AspPro: 3.595 ± 0.067
1.862AspGln: 1.862 ± 0.057
3.648AspArg: 3.648 ± 0.093
3.68AspSer: 3.68 ± 0.098
3.686AspThr: 3.686 ± 0.091
5.324AspVal: 5.324 ± 0.099
0.995AspTrp: 0.995 ± 0.041
1.979AspTyr: 1.979 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
5.679GluAla: 5.679 ± 0.123
0.431GluCys: 0.431 ± 0.027
3.009GluAsp: 3.009 ± 0.078
3.087GluGlu: 3.087 ± 0.085
1.709GluPhe: 1.709 ± 0.054
3.753GluGly: 3.753 ± 0.086
1.681GluHis: 1.681 ± 0.058
2.674GluIle: 2.674 ± 0.072
2.143GluLys: 2.143 ± 0.072
5.096GluLeu: 5.096 ± 0.099
1.331GluMet: 1.331 ± 0.049
1.797GluAsn: 1.797 ± 0.059
2.798GluPro: 2.798 ± 0.091
2.428GluGln: 2.428 ± 0.07
4.27GluArg: 4.27 ± 0.101
3.388GluSer: 3.388 ± 0.064
3.281GluThr: 3.281 ± 0.081
3.481GluVal: 3.481 ± 0.092
0.643GluTrp: 0.643 ± 0.034
1.797GluTyr: 1.797 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
3.787PheAla: 3.787 ± 0.081
0.336PheCys: 0.336 ± 0.026
2.774PheAsp: 2.774 ± 0.07
1.695PheGlu: 1.695 ± 0.059
1.056PhePhe: 1.056 ± 0.051
3.216PheGly: 3.216 ± 0.077
0.71PheHis: 0.71 ± 0.039
1.693PheIle: 1.693 ± 0.064
1.057PheLys: 1.057 ± 0.051
2.411PheLeu: 2.411 ± 0.071
0.745PheMet: 0.745 ± 0.035
1.159PheAsn: 1.159 ± 0.048
1.408PhePro: 1.408 ± 0.046
0.84PheGln: 0.84 ± 0.034
1.644PheArg: 1.644 ± 0.056
2.178PheSer: 2.178 ± 0.064
2.186PheThr: 2.186 ± 0.071
2.578PheVal: 2.578 ± 0.072
0.373PheTrp: 0.373 ± 0.026
0.821PheTyr: 0.821 ± 0.04
0.0PheXaa: 0.0 ± 0.0
Gly
7.99GlyAla: 7.99 ± 0.147
0.82GlyCys: 0.82 ± 0.044
5.496GlyAsp: 5.496 ± 0.099
4.715GlyGlu: 4.715 ± 0.103
3.194GlyPhe: 3.194 ± 0.094
6.544GlyGly: 6.544 ± 0.144
1.794GlyHis: 1.794 ± 0.056
4.551GlyIle: 4.551 ± 0.091
4.098GlyLys: 4.098 ± 0.085
7.114GlyLeu: 7.114 ± 0.131
2.181GlyMet: 2.181 ± 0.062
2.993GlyAsn: 2.993 ± 0.085
2.532GlyPro: 2.532 ± 0.06
2.553GlyGln: 2.553 ± 0.066
5.093GlyArg: 5.093 ± 0.109
5.456GlySer: 5.456 ± 0.108
5.431GlyThr: 5.431 ± 0.12
6.634GlyVal: 6.634 ± 0.132
1.178GlyTrp: 1.178 ± 0.042
2.559GlyTyr: 2.559 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
2.256HisAla: 2.256 ± 0.062
0.233HisCys: 0.233 ± 0.021
1.749HisAsp: 1.749 ± 0.053
1.217HisGlu: 1.217 ± 0.055
0.592HisPhe: 0.592 ± 0.033
2.041HisGly: 2.041 ± 0.065
0.576HisHis: 0.576 ± 0.032
1.188HisIle: 1.188 ± 0.053
0.625HisLys: 0.625 ± 0.031
1.575HisLeu: 1.575 ± 0.051
0.579HisMet: 0.579 ± 0.027
0.574HisAsn: 0.574 ± 0.029
1.276HisPro: 1.276 ± 0.05
0.627HisGln: 0.627 ± 0.032
1.427HisArg: 1.427 ± 0.052
1.107HisSer: 1.107 ± 0.04
1.166HisThr: 1.166 ± 0.044
1.845HisVal: 1.845 ± 0.059
0.301HisTrp: 0.301 ± 0.024
0.585HisTyr: 0.585 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.593IleAla: 6.593 ± 0.101
0.604IleCys: 0.604 ± 0.034
4.241IleAsp: 4.241 ± 0.094
3.065IleGlu: 3.065 ± 0.072
1.317IlePhe: 1.317 ± 0.052
4.598IleGly: 4.598 ± 0.105
0.933IleHis: 0.933 ± 0.041
2.822IleIle: 2.822 ± 0.089
1.689IleLys: 1.689 ± 0.057
3.506IleLeu: 3.506 ± 0.087
1.215IleMet: 1.215 ± 0.048
1.701IleAsn: 1.701 ± 0.057
2.588IlePro: 2.588 ± 0.068
1.247IleGln: 1.247 ± 0.042
3.052IleArg: 3.052 ± 0.084
3.033IleSer: 3.033 ± 0.073
3.232IleThr: 3.232 ± 0.093
4.611IleVal: 4.611 ± 0.105
0.553IleTrp: 0.553 ± 0.027
1.089IleTyr: 1.089 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
5.096LysAla: 5.096 ± 0.126
0.19LysCys: 0.19 ± 0.017
2.487LysAsp: 2.487 ± 0.085
2.057LysGlu: 2.057 ± 0.073
1.041LysPhe: 1.041 ± 0.042
2.859LysGly: 2.859 ± 0.076
0.773LysHis: 0.773 ± 0.033
1.808LysIle: 1.808 ± 0.064
1.873LysLys: 1.873 ± 0.08
3.308LysLeu: 3.308 ± 0.09
0.842LysMet: 0.842 ± 0.036
1.494LysAsn: 1.494 ± 0.061
2.479LysPro: 2.479 ± 0.069
1.448LysGln: 1.448 ± 0.055
2.556LysArg: 2.556 ± 0.057
2.392LysSer: 2.392 ± 0.071
2.843LysThr: 2.843 ± 0.078
2.929LysVal: 2.929 ± 0.086
0.408LysTrp: 0.408 ± 0.029
1.057LysTyr: 1.057 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
9.827LeuAla: 9.827 ± 0.149
0.902LeuCys: 0.902 ± 0.04
6.045LeuAsp: 6.045 ± 0.116
4.016LeuGlu: 4.016 ± 0.087
2.789LeuPhe: 2.789 ± 0.087
6.791LeuGly: 6.791 ± 0.104
1.873LeuHis: 1.873 ± 0.059
4.576LeuIle: 4.576 ± 0.11
3.47LeuLys: 3.47 ± 0.102
7.443LeuLeu: 7.443 ± 0.146
2.224LeuMet: 2.224 ± 0.071
2.803LeuAsn: 2.803 ± 0.069
4.329LeuPro: 4.329 ± 0.102
2.395LeuGln: 2.395 ± 0.071
5.373LeuArg: 5.373 ± 0.113
5.52LeuSer: 5.52 ± 0.11
5.52LeuThr: 5.52 ± 0.093
6.214LeuVal: 6.214 ± 0.138
0.907LeuTrp: 0.907 ± 0.04
1.972LeuTyr: 1.972 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.744MetAla: 2.744 ± 0.07
0.249MetCys: 0.249 ± 0.018
1.438MetAsp: 1.438 ± 0.053
1.079MetGlu: 1.079 ± 0.043
0.837MetPhe: 0.837 ± 0.035
1.894MetGly: 1.894 ± 0.062
0.537MetHis: 0.537 ± 0.027
1.295MetIle: 1.295 ± 0.048
1.028MetLys: 1.028 ± 0.038
2.591MetLeu: 2.591 ± 0.08
0.732MetMet: 0.732 ± 0.039
0.989MetAsn: 0.989 ± 0.037
1.586MetPro: 1.586 ± 0.056
0.896MetGln: 0.896 ± 0.037
1.905MetArg: 1.905 ± 0.065
1.822MetSer: 1.822 ± 0.048
2.039MetThr: 2.039 ± 0.063
1.74MetVal: 1.74 ± 0.051
0.311MetTrp: 0.311 ± 0.025
0.518MetTyr: 0.518 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
3.698AsnAla: 3.698 ± 0.094
0.214AsnCys: 0.214 ± 0.019
2.105AsnAsp: 2.105 ± 0.058
1.696AsnGlu: 1.696 ± 0.057
0.839AsnPhe: 0.839 ± 0.036
3.409AsnGly: 3.409 ± 0.098
0.641AsnHis: 0.641 ± 0.031
1.569AsnIle: 1.569 ± 0.059
1.26AsnLys: 1.26 ± 0.057
2.487AsnLeu: 2.487 ± 0.07
0.737AsnMet: 0.737 ± 0.033
1.148AsnAsn: 1.148 ± 0.056
2.13AsnPro: 2.13 ± 0.062
0.973AsnGln: 0.973 ± 0.036
1.963AsnArg: 1.963 ± 0.061
1.62AsnSer: 1.62 ± 0.059
2.004AsnThr: 2.004 ± 0.069
2.467AsnVal: 2.467 ± 0.075
0.429AsnTrp: 0.429 ± 0.028
0.826AsnTyr: 0.826 ± 0.045
0.0AsnXaa: 0.0 ± 0.0
Pro
5.266ProAla: 5.266 ± 0.11
0.373ProCys: 0.373 ± 0.023
3.567ProAsp: 3.567 ± 0.08
3.286ProGlu: 3.286 ± 0.085
1.644ProPhe: 1.644 ± 0.05
3.884ProGly: 3.884 ± 0.082
0.992ProHis: 0.992 ± 0.045
2.167ProIle: 2.167 ± 0.065
1.854ProLys: 1.854 ± 0.053
3.521ProLeu: 3.521 ± 0.087
1.138ProMet: 1.138 ± 0.046
1.483ProAsn: 1.483 ± 0.045
1.39ProPro: 1.39 ± 0.058
1.952ProGln: 1.952 ± 0.057
2.567ProArg: 2.567 ± 0.075
3.096ProSer: 3.096 ± 0.086
2.801ProThr: 2.801 ± 0.071
4.093ProVal: 4.093 ± 0.093
0.659ProTrp: 0.659 ± 0.036
1.37ProTyr: 1.37 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
3.736GlnAla: 3.736 ± 0.085
0.332GlnCys: 0.332 ± 0.023
1.556GlnAsp: 1.556 ± 0.06
1.539GlnGlu: 1.539 ± 0.05
0.998GlnPhe: 0.998 ± 0.041
2.298GlnGly: 2.298 ± 0.064
0.692GlnHis: 0.692 ± 0.029
1.813GlnIle: 1.813 ± 0.06
1.1GlnLys: 1.1 ± 0.048
3.344GlnLeu: 3.344 ± 0.085
0.928GlnMet: 0.928 ± 0.037
0.914GlnAsn: 0.914 ± 0.043
1.83GlnPro: 1.83 ± 0.076
1.615GlnGln: 1.615 ± 0.066
2.447GlnArg: 2.447 ± 0.073
2.197GlnSer: 2.197 ± 0.063
1.862GlnThr: 1.862 ± 0.064
2.5GlnVal: 2.5 ± 0.062
0.585GlnTrp: 0.585 ± 0.031
1.014GlnTyr: 1.014 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
5.526ArgAla: 5.526 ± 0.116
0.572ArgCys: 0.572 ± 0.032
3.844ArgAsp: 3.844 ± 0.093
3.632ArgGlu: 3.632 ± 0.089
2.409ArgPhe: 2.409 ± 0.065
4.16ArgGly: 4.16 ± 0.097
1.681ArgHis: 1.681 ± 0.055
3.57ArgIle: 3.57 ± 0.083
2.779ArgLys: 2.779 ± 0.079
5.708ArgLeu: 5.708 ± 0.13
2.006ArgMet: 2.006 ± 0.063
1.995ArgAsn: 1.995 ± 0.061
2.798ArgPro: 2.798 ± 0.076
2.392ArgGln: 2.392 ± 0.067
5.378ArgArg: 5.378 ± 0.158
3.426ArgSer: 3.426 ± 0.093
3.367ArgThr: 3.367 ± 0.074
4.407ArgVal: 4.407 ± 0.096
0.885ArgTrp: 0.885 ± 0.042
1.902ArgTyr: 1.902 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
6.737SerAla: 6.737 ± 0.119
0.541SerCys: 0.541 ± 0.035
4.256SerAsp: 4.256 ± 0.099
3.088SerGlu: 3.088 ± 0.068
2.094SerPhe: 2.094 ± 0.067
5.946SerGly: 5.946 ± 0.114
1.357SerHis: 1.357 ± 0.044
3.159SerIle: 3.159 ± 0.075
2.336SerLys: 2.336 ± 0.065
5.126SerLeu: 5.126 ± 0.112
1.749SerMet: 1.749 ± 0.065
1.937SerAsn: 1.937 ± 0.069
2.483SerPro: 2.483 ± 0.058
2.218SerGln: 2.218 ± 0.064
3.792SerArg: 3.792 ± 0.093
4.331SerSer: 4.331 ± 0.114
3.662SerThr: 3.662 ± 0.086
4.665SerVal: 4.665 ± 0.102
0.922SerTrp: 0.922 ± 0.041
1.666SerTyr: 1.666 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
6.888ThrAla: 6.888 ± 0.133
0.526ThrCys: 0.526 ± 0.031
3.969ThrAsp: 3.969 ± 0.098
2.851ThrGlu: 2.851 ± 0.074
2.063ThrPhe: 2.063 ± 0.071
5.617ThrGly: 5.617 ± 0.114
1.156ThrHis: 1.156 ± 0.045
3.324ThrIle: 3.324 ± 0.074
2.234ThrLys: 2.234 ± 0.078
5.566ThrLeu: 5.566 ± 0.102
1.542ThrMet: 1.542 ± 0.052
1.856ThrAsn: 1.856 ± 0.064
3.412ThrPro: 3.412 ± 0.069
1.888ThrGln: 1.888 ± 0.055
3.098ThrArg: 3.098 ± 0.086
3.54ThrSer: 3.54 ± 0.093
3.816ThrThr: 3.816 ± 0.099
5.938ThrVal: 5.938 ± 0.153
0.754ThrTrp: 0.754 ± 0.045
1.657ThrTyr: 1.657 ± 0.068
0.0ThrXaa: 0.0 ± 0.0
Val
8.452ValAla: 8.452 ± 0.144
0.818ValCys: 0.818 ± 0.038
5.142ValAsp: 5.142 ± 0.104
4.241ValGlu: 4.241 ± 0.099
2.827ValPhe: 2.827 ± 0.08
5.319ValGly: 5.319 ± 0.126
1.451ValHis: 1.451 ± 0.055
4.305ValIle: 4.305 ± 0.099
3.116ValLys: 3.116 ± 0.087
6.847ValLeu: 6.847 ± 0.124
2.089ValMet: 2.089 ± 0.069
2.698ValAsn: 2.698 ± 0.074
3.961ValPro: 3.961 ± 0.088
2.058ValGln: 2.058 ± 0.055
4.622ValArg: 4.622 ± 0.091
5.471ValSer: 5.471 ± 0.1
5.592ValThr: 5.592 ± 0.134
6.406ValVal: 6.406 ± 0.124
0.944ValTrp: 0.944 ± 0.04
1.787ValTyr: 1.787 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
1.107TrpAla: 1.107 ± 0.04
0.136TrpCys: 0.136 ± 0.013
0.751TrpAsp: 0.751 ± 0.033
0.534TrpGlu: 0.534 ± 0.031
0.548TrpPhe: 0.548 ± 0.034
0.887TrpGly: 0.887 ± 0.037
0.327TrpHis: 0.327 ± 0.022
0.718TrpIle: 0.718 ± 0.035
0.574TrpLys: 0.574 ± 0.033
1.322TrpLeu: 1.322 ± 0.055
0.432TrpMet: 0.432 ± 0.03
0.569TrpAsn: 0.569 ± 0.035
0.564TrpPro: 0.564 ± 0.035
0.548TrpGln: 0.548 ± 0.032
0.941TrpArg: 0.941 ± 0.042
0.832TrpSer: 0.832 ± 0.039
0.839TrpThr: 0.839 ± 0.039
0.808TrpVal: 0.808 ± 0.039
0.225TrpTrp: 0.225 ± 0.019
0.387TrpTyr: 0.387 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.846TyrAla: 2.846 ± 0.068
0.297TyrCys: 0.297 ± 0.023
1.94TyrAsp: 1.94 ± 0.058
1.556TyrGlu: 1.556 ± 0.05
0.898TyrPhe: 0.898 ± 0.043
2.514TyrGly: 2.514 ± 0.066
0.517TyrHis: 0.517 ± 0.028
1.148TyrIle: 1.148 ± 0.043
0.883TyrLys: 0.883 ± 0.042
2.2TyrLeu: 2.2 ± 0.055
0.566TyrMet: 0.566 ± 0.03
0.783TyrAsn: 0.783 ± 0.04
1.17TyrPro: 1.17 ± 0.043
0.947TyrGln: 0.947 ± 0.038
1.883TyrArg: 1.883 ± 0.071
1.63TyrSer: 1.63 ± 0.057
1.668TyrThr: 1.668 ± 0.069
2.017TyrVal: 2.017 ± 0.056
0.384TyrTrp: 0.384 ± 0.031
0.743TyrTyr: 0.743 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1834 proteins (627178 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski