Vibrio genomosp. F10
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3193 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B9R1J0|A0A1B9R1J0_9VIBR Uncharacterized protein OS=Vibrio genomosp. F10 OX=723171 GN=A6E14_06865 PE=4 SV=1
MM1 pKa = 7.35 FNIKK5 pKa = 9.63 SLPMAICLGLAALSGSAASASAMAGEE31 pKa = 4.58 MVNPDD36 pKa = 3.05 GNVVVGYY43 pKa = 6.91 WHH45 pKa = 6.68 NWCDD49 pKa = 3.29 GAGYY53 pKa = 10.39 KK54 pKa = 10.38 GGNSPCMSLDD64 pKa = 5.11 DD65 pKa = 4.69 INPMYY70 pKa = 10.82 NVVDD74 pKa = 3.7 ISFMKK79 pKa = 10.7 VYY81 pKa = 9.18 DD82 pKa = 3.84 TAEE85 pKa = 3.76 GRR87 pKa = 11.84 IPTFKK92 pKa = 10.76 LDD94 pKa = 3.54 PTVGLSEE101 pKa = 4.36 QEE103 pKa = 4.48 FINQIEE109 pKa = 4.22 QLNQQGRR116 pKa = 11.84 SVLLALGGADD126 pKa = 3.84 AHH128 pKa = 7.43 VEE130 pKa = 4.0 LQKK133 pKa = 11.17 GDD135 pKa = 3.51 EE136 pKa = 3.97 QAFAEE141 pKa = 4.64 EE142 pKa = 4.77 IIRR145 pKa = 11.84 LTEE148 pKa = 3.62 QYY150 pKa = 11.18 GFDD153 pKa = 3.96 GLDD156 pKa = 3.07 IDD158 pKa = 5.36 LEE160 pKa = 4.37 QAAVTAADD168 pKa = 3.71 NQIVIPAALKK178 pKa = 10.05 IVKK181 pKa = 9.44 DD182 pKa = 3.73 HH183 pKa = 6.42 YY184 pKa = 11.11 AAEE187 pKa = 4.54 GKK189 pKa = 10.17 NFLITMAPEE198 pKa = 4.36 FPYY201 pKa = 9.54 LTQGGKK207 pKa = 9.32 YY208 pKa = 9.76 VPYY211 pKa = 9.86 IDD213 pKa = 5.19 NLAGYY218 pKa = 8.89 YY219 pKa = 10.41 DD220 pKa = 4.32 WINPQFYY227 pKa = 10.24 NQGGDD232 pKa = 4.12 GIWVDD237 pKa = 3.71 SVGWIAQNNDD247 pKa = 2.84 ALKK250 pKa = 10.7 QEE252 pKa = 4.84 FIYY255 pKa = 10.92 YY256 pKa = 9.95 IADD259 pKa = 3.49 SLSNGTRR266 pKa = 11.84 GFHH269 pKa = 6.38 QIPHH273 pKa = 6.11 NKK275 pKa = 9.59 LVFGIPANNDD285 pKa = 2.95 AAATGYY291 pKa = 8.94 VQNPQDD297 pKa = 4.1 LYY299 pKa = 11.63 DD300 pKa = 4.74 AFEE303 pKa = 4.0 QLNQQGQPLRR313 pKa = 11.84 GVMTWSVNWDD323 pKa = 3.25 MGTDD327 pKa = 3.34 AGGRR331 pKa = 11.84 AYY333 pKa = 10.74 NEE335 pKa = 3.68 QFIRR339 pKa = 11.84 DD340 pKa = 4.1 YY341 pKa = 11.24 GPYY344 pKa = 9.56 IHH346 pKa = 6.94 GQTPPPITSNEE357 pKa = 3.94 PVLKK361 pKa = 10.82 GIDD364 pKa = 3.73 DD365 pKa = 3.83 ARR367 pKa = 11.84 VAHH370 pKa = 6.1 QSYY373 pKa = 9.42 FDD375 pKa = 3.82 PMSDD379 pKa = 3.07 VTAMDD384 pKa = 5.38 DD385 pKa = 3.49 EE386 pKa = 7.32 DD387 pKa = 6.6 GDD389 pKa = 4.2 LTHH392 pKa = 7.33 HH393 pKa = 6.2 ITVEE397 pKa = 4.26 GQVNTSSVGTYY408 pKa = 10.5 ALTYY412 pKa = 10.36 LVEE415 pKa = 5.81 DD416 pKa = 3.53 SDD418 pKa = 4.75 GNQTSKK424 pKa = 10.44 VRR426 pKa = 11.84 SVDD429 pKa = 3.62 VYY431 pKa = 10.51 SSKK434 pKa = 10.92 PIFKK438 pKa = 10.23 GVSDD442 pKa = 3.55 TSIQFGSEE450 pKa = 3.73 FDD452 pKa = 3.67 SLAGVSASDD461 pKa = 4.53 EE462 pKa = 4.18 EE463 pKa = 6.15 DD464 pKa = 3.49 GDD466 pKa = 3.8 LTSRR470 pKa = 11.84 IEE472 pKa = 3.79 IYY474 pKa = 11.17 GDD476 pKa = 3.44 VEE478 pKa = 4.38 TNRR481 pKa = 11.84 LGSQTLLYY489 pKa = 10.77 SVTDD493 pKa = 3.59 SANQSTTVEE502 pKa = 4.15 RR503 pKa = 11.84 TIHH506 pKa = 5.97 VVDD509 pKa = 5.05 EE510 pKa = 4.68 SSCAAPWQTNNVYY523 pKa = 10.58 EE524 pKa = 4.56 GGDD527 pKa = 3.31 RR528 pKa = 11.84 VLHH531 pKa = 6.85 DD532 pKa = 3.39 GTVYY536 pKa = 10.03 EE537 pKa = 4.29 AAWWTRR543 pKa = 11.84 GEE545 pKa = 4.29 QPGSTGQWGVWRR557 pKa = 11.84 EE558 pKa = 4.05 VTDD561 pKa = 3.7 TGCDD565 pKa = 3.32 GTNNGDD571 pKa = 3.73 NGAGDD576 pKa = 4.54 DD577 pKa = 5.13 NNNGDD582 pKa = 4.29 NNNSGDD588 pKa = 3.86 TDD590 pKa = 3.69 NGSDD594 pKa = 3.36 TGNEE598 pKa = 4.01 NGDD601 pKa = 3.97 SNGGNGNTDD610 pKa = 3.82 NVTEE614 pKa = 5.07 WIPGQTQASNGDD626 pKa = 3.52 MVSYY630 pKa = 10.5 QGSCFVAKK638 pKa = 10.4 NNPGVWEE645 pKa = 4.61 TPTQSATWFWDD656 pKa = 3.75 EE657 pKa = 4.36 VSCPP661 pKa = 3.51
Molecular weight: 71.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.923
IPC_protein 3.948
Toseland 3.732
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.872
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.304
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.075
Patrickios 1.138
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.953
Protein with the highest isoelectric point:
>tr|A0A1B9R0H4|A0A1B9R0H4_9VIBR PBPb domain-containing protein OS=Vibrio genomosp. F10 OX=723171 GN=A6E14_01210 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.65 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 ATINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSKK44 pKa = 10.84
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3193
0
3193
1029883
35
1908
322.5
35.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.446 ± 0.043
0.994 ± 0.013
5.572 ± 0.038
6.376 ± 0.041
4.086 ± 0.03
6.871 ± 0.043
2.283 ± 0.022
6.514 ± 0.034
5.236 ± 0.034
10.357 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.705 ± 0.021
4.196 ± 0.03
3.855 ± 0.027
4.508 ± 0.031
4.478 ± 0.033
6.871 ± 0.039
5.422 ± 0.031
7.129 ± 0.038
1.221 ± 0.016
2.881 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here