bacterium HR17

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2781 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5XAE8|A0A2H5XAE8_9BACT Uncharacterized protein OS=bacterium HR17 OX=2035412 GN=HRbin17_00659 PE=4 SV=1
MM1 pKa = 7.73AKK3 pKa = 10.44GSLSVMLCLVGGFKK17 pKa = 10.44EE18 pKa = 4.66SEE20 pKa = 4.28GSQPSYY26 pKa = 10.35YY27 pKa = 9.74PPYY30 pKa = 9.88PPPQHH35 pKa = 4.81QHH37 pKa = 4.22NWTAGSSIPKK47 pKa = 8.72PIVMLFYY54 pKa = 10.5PGTSYY59 pKa = 10.48TLTHH63 pKa = 6.23LRR65 pKa = 11.84CYY67 pKa = 10.27EE68 pKa = 3.8IAFLADD74 pKa = 3.18VASATDD80 pKa = 3.68WDD82 pKa = 3.7QCIAPGCPYY91 pKa = 10.32PNGWAADD98 pKa = 3.65SKK100 pKa = 10.86VRR102 pKa = 11.84VMWSDD107 pKa = 3.12NGAGGQFGTLDD118 pKa = 3.55SNGNFIPLDD127 pKa = 3.59PVVNAQQITHH137 pKa = 5.98YY138 pKa = 9.63RR139 pKa = 11.84APWDD143 pKa = 3.45RR144 pKa = 11.84PGIVHH149 pKa = 6.27ITLVVDD155 pKa = 5.46DD156 pKa = 4.89EE157 pKa = 4.41GLYY160 pKa = 10.81YY161 pKa = 10.41DD162 pKa = 5.7DD163 pKa = 5.64PPVSNNSDD171 pKa = 3.45NGNVTVWEE179 pKa = 4.5FWITPCPYY187 pKa = 9.62NWRR190 pKa = 11.84PIPNTTGPSFIAWVEE205 pKa = 3.97PEE207 pKa = 4.13VDD209 pKa = 3.47HH210 pKa = 7.1LGQPMSGIMTFNLISSTEE228 pKa = 3.84PGEE231 pKa = 4.2CLNSHH236 pKa = 6.87FSCPYY241 pKa = 11.15DD242 pKa = 3.37MDD244 pKa = 3.48IVQTEE249 pKa = 3.97EE250 pKa = 4.64GEE252 pKa = 4.4TFEE255 pKa = 5.31VNDD258 pKa = 4.05IHH260 pKa = 8.16DD261 pKa = 4.85HH262 pKa = 6.8DD263 pKa = 6.22ADD265 pKa = 3.87LQFPPEE271 pKa = 4.1LEE273 pKa = 3.92GMNVYY278 pKa = 10.28GSQEE282 pKa = 3.78EE283 pKa = 4.65DD284 pKa = 3.27EE285 pKa = 4.77EE286 pKa = 5.28GPYY289 pKa = 8.64THH291 pKa = 6.96NFTVAVTQQPTTVAEE306 pKa = 4.05VRR308 pKa = 11.84ILCFDD313 pKa = 3.53GGGYY317 pKa = 10.75GFLLAGIDD325 pKa = 3.85GFPPGSATARR335 pKa = 11.84RR336 pKa = 11.84TDD338 pKa = 3.13TGVIGSVEE346 pKa = 3.78IPYY349 pKa = 9.99DD350 pKa = 3.56ANGNFIADD358 pKa = 3.19VWEE361 pKa = 4.45ANMGIYY367 pKa = 9.77PADD370 pKa = 3.51SLGDD374 pKa = 3.61FDD376 pKa = 4.64NQPVGDD382 pKa = 4.03GTLGDD387 pKa = 3.74GLSVYY392 pKa = 10.33EE393 pKa = 4.4EE394 pKa = 4.01YY395 pKa = 10.58RR396 pKa = 11.84GLRR399 pKa = 11.84VQGIWTVFNPRR410 pKa = 11.84LKK412 pKa = 11.26DD413 pKa = 3.24MFVMNFGAGQEE424 pKa = 4.2DD425 pKa = 5.53PYY427 pKa = 11.76VPATIPNEE435 pKa = 4.47AITSQDD441 pKa = 3.13GFPGQGMPLLWLLNARR457 pKa = 11.84EE458 pKa = 4.07GLGYY462 pKa = 9.21PLRR465 pKa = 11.84VVNYY469 pKa = 8.78LNHH472 pKa = 6.15YY473 pKa = 8.8AHH475 pKa = 6.86IRR477 pKa = 11.84DD478 pKa = 3.61VYY480 pKa = 10.78AAIVVPGDD488 pKa = 3.12IEE490 pKa = 4.92GYY492 pKa = 10.27QDD494 pKa = 4.45ADD496 pKa = 3.71NDD498 pKa = 4.3GQPDD502 pKa = 3.8DD503 pKa = 4.13TNGDD507 pKa = 3.79GVIDD511 pKa = 4.71DD512 pKa = 4.52SDD514 pKa = 5.4LIVDD518 pKa = 3.55VRR520 pKa = 11.84AYY522 pKa = 10.44GFTYY526 pKa = 10.47GPIWDD531 pKa = 3.99NAGGPIAPPGDD542 pKa = 3.17WGYY545 pKa = 10.61IPQFWGPPVIEE556 pKa = 4.15VDD558 pKa = 2.92VDD560 pKa = 3.3YY561 pKa = 11.39LRR563 pKa = 11.84NEE565 pKa = 4.07RR566 pKa = 11.84LDD568 pKa = 3.93PNTNPNGLTEE578 pKa = 4.26LQVLTWVVGHH588 pKa = 6.03EE589 pKa = 4.32LGHH592 pKa = 5.27TVLWHH597 pKa = 6.34RR598 pKa = 11.84NDD600 pKa = 3.33LGGYY604 pKa = 6.92GHH606 pKa = 7.28HH607 pKa = 6.96NGFDD611 pKa = 3.42AAGNPITTYY620 pKa = 11.25DD621 pKa = 3.69CLIWQWMDD629 pKa = 2.56WRR631 pKa = 11.84ANPPTEE637 pKa = 4.06FCAVNPGDD645 pKa = 3.55QTRR648 pKa = 11.84WKK650 pKa = 10.24INPP653 pKa = 3.5

Molecular weight: 72.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5XEA0|A0A2H5XEA0_9BACT ECF RNA polymerase sigma-E factor OS=bacterium HR17 OX=2035412 GN=rpoE_4 PE=3 SV=1
MM1 pKa = 7.82WKK3 pKa = 10.64RR4 pKa = 11.84MMAWLLAGTMTLPVAVWAQQPSKK27 pKa = 11.09KK28 pKa = 8.65PTQPPAKK35 pKa = 9.12KK36 pKa = 9.95AQPAKK41 pKa = 9.56PAQPAKK47 pKa = 9.61PATPATPAKK56 pKa = 9.22PATPATPAKK65 pKa = 9.49PATPAQPATPAKK77 pKa = 10.08KK78 pKa = 10.13AATGTKK84 pKa = 9.11KK85 pKa = 10.14RR86 pKa = 11.84AAKK89 pKa = 9.66RR90 pKa = 11.84HH91 pKa = 4.11VRR93 pKa = 11.84KK94 pKa = 9.39HH95 pKa = 5.12RR96 pKa = 11.84KK97 pKa = 7.6VRR99 pKa = 11.84HH100 pKa = 5.49RR101 pKa = 11.84RR102 pKa = 11.84PAHH105 pKa = 6.03RR106 pKa = 11.84RR107 pKa = 11.84TTQKK111 pKa = 10.29VAAAKK116 pKa = 10.14KK117 pKa = 9.78

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2781

0

2781

939222

29

2022

337.7

37.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.825 ± 0.059

1.164 ± 0.016

4.859 ± 0.031

5.852 ± 0.051

3.851 ± 0.032

7.441 ± 0.042

2.299 ± 0.022

4.274 ± 0.031

3.172 ± 0.033

11.111 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.022 ± 0.019

2.39 ± 0.022

5.862 ± 0.04

4.134 ± 0.032

8.064 ± 0.042

4.035 ± 0.028

5.41 ± 0.034

8.782 ± 0.044

2.163 ± 0.027

2.291 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski