Amino acid dipepetide frequency for bacterium HR17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.059AlaAla: 12.059 ± 0.181	1.135AlaCys: 1.135 ± 0.038	5.054AlaAsp: 5.054 ± 0.076	6.633AlaGlu: 6.633 ± 0.101	4.595AlaPhe: 4.595 ± 0.074	6.827AlaGly: 6.827 ± 0.09	2.433AlaHis: 2.433 ± 0.052	5.056AlaIle: 5.056 ± 0.088	3.596AlaLys: 3.596 ± 0.073	14.57AlaLeu: 14.57 ± 0.164	2.609AlaMet: 2.609 ± 0.063	2.257AlaAsn: 2.257 ± 0.057	5.507AlaPro: 5.507 ± 0.1	5.146AlaGln: 5.146 ± 0.096	6.717AlaArg: 6.717 ± 0.09	3.909AlaSer: 3.909 ± 0.072	5.601AlaThr: 5.601 ± 0.087	9.841AlaVal: 9.841 ± 0.116	2.232AlaTrp: 2.232 ± 0.05	2.472AlaTyr: 2.472 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.14CysAla: 1.14 ± 0.035	0.208CysCys: 0.208 ± 0.018	0.679CysAsp: 0.679 ± 0.03	0.569CysGlu: 0.569 ± 0.025	0.415CysPhe: 0.415 ± 0.022	1.181CysGly: 1.181 ± 0.039	0.444CysHis: 0.444 ± 0.044	0.422CysIle: 0.422 ± 0.025	0.247CysLys: 0.247 ± 0.016	1.216CysLeu: 1.216 ± 0.04	0.137CysMet: 0.137 ± 0.012	0.234CysAsn: 0.234 ± 0.017	0.906CysPro: 0.906 ± 0.037	0.422CysGln: 0.422 ± 0.02	0.917CysArg: 0.917 ± 0.036	0.415CysSer: 0.415 ± 0.02	0.451CysThr: 0.451 ± 0.022	1.05CysVal: 1.05 ± 0.033	0.264CysTrp: 0.264 ± 0.017	0.32CysTyr: 0.32 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	5.977AspAla: 5.977 ± 0.099	0.497AspCys: 0.497 ± 0.022	2.793AspAsp: 2.793 ± 0.063	4.213AspGlu: 4.213 ± 0.075	1.846AspPhe: 1.846 ± 0.05	4.952AspGly: 4.952 ± 0.092	0.794AspHis: 0.794 ± 0.026	2.66AspIle: 2.66 ± 0.054	1.512AspLys: 1.512 ± 0.042	4.679AspLeu: 4.679 ± 0.067	0.768AspMet: 0.768 ± 0.029	1.115AspAsn: 1.115 ± 0.035	3.34AspPro: 3.34 ± 0.074	1.013AspGln: 1.013 ± 0.032	3.339AspArg: 3.339 ± 0.059	1.409AspSer: 1.409 ± 0.036	2.118AspThr: 2.118 ± 0.047	3.0AspVal: 3.0 ± 0.063	1.188AspTrp: 1.188 ± 0.037	1.376AspTyr: 1.376 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	5.996GluAla: 5.996 ± 0.093	0.527GluCys: 0.527 ± 0.023	1.855GluAsp: 1.855 ± 0.049	3.291GluGlu: 3.291 ± 0.09	2.066GluPhe: 2.066 ± 0.05	4.272GluGly: 4.272 ± 0.068	1.433GluHis: 1.433 ± 0.044	2.579GluIle: 2.579 ± 0.06	2.042GluLys: 2.042 ± 0.055	6.291GluLeu: 6.291 ± 0.088	1.291GluMet: 1.291 ± 0.041	1.202GluAsn: 1.202 ± 0.04	3.21GluPro: 3.21 ± 0.056	2.884GluGln: 2.884 ± 0.067	7.095GluArg: 7.095 ± 0.109	1.841GluSer: 1.841 ± 0.048	3.124GluThr: 3.124 ± 0.055	5.353GluVal: 5.353 ± 0.078	1.366GluTrp: 1.366 ± 0.035	0.803GluTyr: 0.803 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.783PheAla: 4.783 ± 0.081	0.602PheCys: 0.602 ± 0.024	1.908PheAsp: 1.908 ± 0.045	1.897PheGlu: 1.897 ± 0.051	1.558PhePhe: 1.558 ± 0.054	3.31PheGly: 3.31 ± 0.072	0.807PheHis: 0.807 ± 0.032	1.54PheIle: 1.54 ± 0.044	0.837PheLys: 0.837 ± 0.032	3.757PheLeu: 3.757 ± 0.077	0.546PheMet: 0.546 ± 0.025	1.083PheAsn: 1.083 ± 0.035	2.152PhePro: 2.152 ± 0.046	1.092PheGln: 1.092 ± 0.034	2.576PheArg: 2.576 ± 0.05	1.725PheSer: 1.725 ± 0.041	2.128PheThr: 2.128 ± 0.051	4.251PheVal: 4.251 ± 0.071	0.934PheTrp: 0.934 ± 0.036	1.03PheTyr: 1.03 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	7.271GlyAla: 7.271 ± 0.114	1.03GlyCys: 1.03 ± 0.036	3.905GlyAsp: 3.905 ± 0.073	4.738GlyGlu: 4.738 ± 0.081	3.069GlyPhe: 3.069 ± 0.071	5.655GlyGly: 5.655 ± 0.109	1.658GlyHis: 1.658 ± 0.044	4.003GlyIle: 4.003 ± 0.069	3.589GlyLys: 3.589 ± 0.069	6.852GlyLeu: 6.852 ± 0.095	1.845GlyMet: 1.845 ± 0.052	2.066GlyAsn: 2.066 ± 0.059	1.77GlyPro: 1.77 ± 0.05	2.876GlyGln: 2.876 ± 0.06	5.046GlyArg: 5.046 ± 0.072	3.209GlySer: 3.209 ± 0.064	4.42GlyThr: 4.42 ± 0.069	7.249GlyVal: 7.249 ± 0.102	1.801GlyTrp: 1.801 ± 0.051	2.363GlyTyr: 2.363 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	2.339HisAla: 2.339 ± 0.056	0.345HisCys: 0.345 ± 0.02	1.074HisAsp: 1.074 ± 0.038	1.24HisGlu: 1.24 ± 0.037	1.026HisPhe: 1.026 ± 0.036	1.737HisGly: 1.737 ± 0.044	0.67HisHis: 0.67 ± 0.027	1.241HisIle: 1.241 ± 0.037	0.586HisLys: 0.586 ± 0.024	2.799HisLeu: 2.799 ± 0.051	0.368HisMet: 0.368 ± 0.019	0.573HisAsn: 0.573 ± 0.025	1.912HisPro: 1.912 ± 0.053	0.833HisGln: 0.833 ± 0.029	2.053HisArg: 2.053 ± 0.052	0.812HisSer: 0.812 ± 0.03	1.041HisThr: 1.041 ± 0.035	1.047HisVal: 1.047 ± 0.034	0.588HisTrp: 0.588 ± 0.026	0.704HisTyr: 0.704 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	7.137IleAla: 7.137 ± 0.093	0.503IleCys: 0.503 ± 0.026	1.91IleAsp: 1.91 ± 0.049	2.217IleGlu: 2.217 ± 0.049	1.347IlePhe: 1.347 ± 0.042	4.183IleGly: 4.183 ± 0.069	0.768IleHis: 0.768 ± 0.029	1.468IleIle: 1.468 ± 0.048	1.218IleLys: 1.218 ± 0.039	3.563IleLeu: 3.563 ± 0.064	0.509IleMet: 0.509 ± 0.025	1.148IleAsn: 1.148 ± 0.035	2.173IlePro: 2.173 ± 0.054	1.065IleGln: 1.065 ± 0.032	2.684IleArg: 2.684 ± 0.056	1.678IleSer: 1.678 ± 0.044	2.171IleThr: 2.171 ± 0.049	5.255IleVal: 5.255 ± 0.077	0.682IleTrp: 0.682 ± 0.027	1.067IleTyr: 1.067 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	3.778LysAla: 3.778 ± 0.063	0.258LysCys: 0.258 ± 0.017	1.422LysAsp: 1.422 ± 0.039	2.072LysGlu: 2.072 ± 0.061	1.008LysPhe: 1.008 ± 0.04	3.0LysGly: 3.0 ± 0.066	0.598LysHis: 0.598 ± 0.025	1.081LysIle: 1.081 ± 0.038	0.792LysLys: 0.792 ± 0.033	2.942LysLeu: 2.942 ± 0.067	0.651LysMet: 0.651 ± 0.025	0.59LysAsn: 0.59 ± 0.03	2.192LysPro: 2.192 ± 0.047	1.125LysGln: 1.125 ± 0.039	2.8LysArg: 2.8 ± 0.058	1.251LysSer: 1.251 ± 0.045	1.706LysThr: 1.706 ± 0.044	3.467LysVal: 3.467 ± 0.064	0.586LysTrp: 0.586 ± 0.025	0.399LysTyr: 0.399 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	11.662LeuAla: 11.662 ± 0.161	1.379LeuCys: 1.379 ± 0.039	4.717LeuAsp: 4.717 ± 0.067	5.464LeuGlu: 5.464 ± 0.089	3.977LeuPhe: 3.977 ± 0.077	7.73LeuGly: 7.73 ± 0.111	2.465LeuHis: 2.465 ± 0.051	4.2LeuIle: 4.2 ± 0.07	3.621LeuLys: 3.621 ± 0.061	12.803LeuLeu: 12.803 ± 0.172	2.402LeuMet: 2.402 ± 0.051	2.652LeuAsn: 2.652 ± 0.058	7.452LeuPro: 7.452 ± 0.108	4.972LeuGln: 4.972 ± 0.085	10.187LeuArg: 10.187 ± 0.126	5.522LeuSer: 5.522 ± 0.084	6.828LeuThr: 6.828 ± 0.088	8.588LeuVal: 8.588 ± 0.114	2.344LeuTrp: 2.344 ± 0.059	2.145LeuTyr: 2.145 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.594MetAla: 2.594 ± 0.051	0.171MetCys: 0.171 ± 0.014	0.986MetAsp: 0.986 ± 0.031	1.307MetGlu: 1.307 ± 0.033	0.541MetPhe: 0.541 ± 0.024	1.714MetGly: 1.714 ± 0.05	0.409MetHis: 0.409 ± 0.023	0.406MetIle: 0.406 ± 0.021	0.652MetLys: 0.652 ± 0.034	2.023MetLeu: 2.023 ± 0.057	0.433MetMet: 0.433 ± 0.02	0.445MetAsn: 0.445 ± 0.023	1.37MetPro: 1.37 ± 0.043	0.802MetGln: 0.802 ± 0.034	1.974MetArg: 1.974 ± 0.044	0.807MetSer: 0.807 ± 0.026	1.316MetThr: 1.316 ± 0.039	1.783MetVal: 1.783 ± 0.048	0.302MetTrp: 0.302 ± 0.017	0.181MetTyr: 0.181 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.993AsnAla: 2.993 ± 0.063	0.357AsnCys: 0.357 ± 0.02	1.237AsnAsp: 1.237 ± 0.038	1.446AsnGlu: 1.446 ± 0.041	1.087AsnPhe: 1.087 ± 0.035	2.29AsnGly: 2.29 ± 0.05	0.398AsnHis: 0.398 ± 0.022	1.224AsnIle: 1.224 ± 0.036	0.571AsnLys: 0.571 ± 0.023	2.76AsnLeu: 2.76 ± 0.057	0.398AsnMet: 0.398 ± 0.022	0.582AsnAsn: 0.582 ± 0.031	2.02AsnPro: 2.02 ± 0.052	0.58AsnGln: 0.58 ± 0.026	1.743AsnArg: 1.743 ± 0.042	0.793AsnSer: 0.793 ± 0.032	1.025AsnThr: 1.025 ± 0.037	1.159AsnVal: 1.159 ± 0.035	0.564AsnTrp: 0.564 ± 0.029	0.667AsnTyr: 0.667 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	4.873ProAla: 4.873 ± 0.086	0.564ProCys: 0.564 ± 0.028	3.92ProAsp: 3.92 ± 0.066	3.739ProGlu: 3.739 ± 0.068	2.63ProPhe: 2.63 ± 0.058	2.614ProGly: 2.614 ± 0.072	1.653ProHis: 1.653 ± 0.047	2.506ProIle: 2.506 ± 0.052	2.02ProLys: 2.02 ± 0.058	6.546ProLeu: 6.546 ± 0.087	1.434ProMet: 1.434 ± 0.043	1.883ProAsn: 1.883 ± 0.054	4.443ProPro: 4.443 ± 0.088	2.68ProGln: 2.68 ± 0.056	3.204ProArg: 3.204 ± 0.064	3.533ProSer: 3.533 ± 0.086	3.731ProThr: 3.731 ± 0.066	4.568ProVal: 4.568 ± 0.074	0.652ProTrp: 0.652 ± 0.029	1.428ProTyr: 1.428 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	4.235GlnAla: 4.235 ± 0.092	0.394GlnCys: 0.394 ± 0.021	1.227GlnAsp: 1.227 ± 0.033	2.057GlnGlu: 2.057 ± 0.056	1.453GlnPhe: 1.453 ± 0.039	2.76GlnGly: 2.76 ± 0.062	0.866GlnHis: 0.866 ± 0.037	1.783GlnIle: 1.783 ± 0.043	1.431GlnLys: 1.431 ± 0.044	4.446GlnLeu: 4.446 ± 0.082	1.004GlnMet: 1.004 ± 0.034	0.832GlnAsn: 0.832 ± 0.033	2.59GlnPro: 2.59 ± 0.072	2.179GlnGln: 2.179 ± 0.072	4.737GlnArg: 4.737 ± 0.081	1.567GlnSer: 1.567 ± 0.042	2.36GlnThr: 2.36 ± 0.058	3.793GlnVal: 3.793 ± 0.061	1.113GlnTrp: 1.113 ± 0.032	0.511GlnTyr: 0.511 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	6.783ArgAla: 6.783 ± 0.086	1.138ArgCys: 1.138 ± 0.039	3.768ArgAsp: 3.768 ± 0.06	4.804ArgGlu: 4.804 ± 0.079	3.556ArgPhe: 3.556 ± 0.062	4.448ArgGly: 4.448 ± 0.068	2.142ArgHis: 2.142 ± 0.049	3.688ArgIle: 3.688 ± 0.07	2.717ArgLys: 2.717 ± 0.058	10.044ArgLeu: 10.044 ± 0.132	1.847ArgMet: 1.847 ± 0.043	1.911ArgAsn: 1.911 ± 0.047	3.946ArgPro: 3.946 ± 0.064	4.437ArgGln: 4.437 ± 0.078	7.364ArgArg: 7.364 ± 0.107	3.165ArgSer: 3.165 ± 0.069	3.711ArgThr: 3.711 ± 0.071	5.937ArgVal: 5.937 ± 0.086	2.642ArgTrp: 2.642 ± 0.063	2.593ArgTyr: 2.593 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	4.239SerAla: 4.239 ± 0.077	0.428SerCys: 0.428 ± 0.022	2.395SerAsp: 2.395 ± 0.051	2.607SerGlu: 2.607 ± 0.063	1.543SerPhe: 1.543 ± 0.043	3.607SerGly: 3.607 ± 0.064	0.92SerHis: 0.92 ± 0.031	1.463SerIle: 1.463 ± 0.038	1.125SerLys: 1.125 ± 0.042	4.432SerLeu: 4.432 ± 0.081	0.627SerMet: 0.627 ± 0.024	0.868SerAsn: 0.868 ± 0.029	2.928SerPro: 2.928 ± 0.06	1.465SerGln: 1.465 ± 0.042	2.549SerArg: 2.549 ± 0.056	1.7SerSer: 1.7 ± 0.046	1.695SerThr: 1.695 ± 0.041	4.112SerVal: 4.112 ± 0.062	0.75SerTrp: 0.75 ± 0.031	0.896SerTyr: 0.896 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	6.057ThrAla: 6.057 ± 0.088	0.48ThrCys: 0.48 ± 0.024	2.974ThrAsp: 2.974 ± 0.049	2.978ThrGlu: 2.978 ± 0.054	1.995ThrPhe: 1.995 ± 0.047	4.305ThrGly: 4.305 ± 0.069	1.278ThrHis: 1.278 ± 0.043	2.011ThrIle: 2.011 ± 0.052	1.542ThrLys: 1.542 ± 0.041	6.446ThrLeu: 6.446 ± 0.091	1.055ThrMet: 1.055 ± 0.031	1.204ThrAsn: 1.204 ± 0.041	4.012ThrPro: 4.012 ± 0.072	1.919ThrGln: 1.919 ± 0.047	2.938ThrArg: 2.938 ± 0.053	1.677ThrSer: 1.677 ± 0.048	3.01ThrThr: 3.01 ± 0.067	6.223ThrVal: 6.223 ± 0.106	0.753ThrTrp: 0.753 ± 0.025	1.245ThrTyr: 1.245 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	9.872ValAla: 9.872 ± 0.119	0.978ValCys: 0.978 ± 0.036	4.221ValAsp: 4.221 ± 0.064	4.719ValGlu: 4.719 ± 0.077	3.028ValPhe: 3.028 ± 0.056	6.336ValGly: 6.336 ± 0.088	1.981ValHis: 1.981 ± 0.047	4.052ValIle: 4.052 ± 0.067	2.658ValLys: 2.658 ± 0.061	8.977ValLeu: 8.977 ± 0.099	1.545ValMet: 1.545 ± 0.047	2.287ValAsn: 2.287 ± 0.058	5.149ValPro: 5.149 ± 0.082	3.423ValGln: 3.423 ± 0.07	7.733ValArg: 7.733 ± 0.092	3.691ValSer: 3.691 ± 0.064	5.55ValThr: 5.55 ± 0.08	8.064ValVal: 8.064 ± 0.109	1.725ValTrp: 1.725 ± 0.048	1.832ValTyr: 1.832 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	1.925TrpAla: 1.925 ± 0.052	0.316TrpCys: 0.316 ± 0.018	1.053TrpAsp: 1.053 ± 0.038	1.11TrpGlu: 1.11 ± 0.032	0.812TrpPhe: 0.812 ± 0.034	1.528TrpGly: 1.528 ± 0.045	0.686TrpHis: 0.686 ± 0.028	0.477TrpIle: 0.477 ± 0.028	0.464TrpLys: 0.464 ± 0.022	3.298TrpLeu: 3.298 ± 0.071	0.43TrpMet: 0.43 ± 0.019	0.459TrpAsn: 0.459 ± 0.024	0.174TrpPro: 0.174 ± 0.014	1.672TrpGln: 1.672 ± 0.05	2.571TrpArg: 2.571 ± 0.055	0.852TrpSer: 0.852 ± 0.032	1.043TrpThr: 1.043 ± 0.039	1.846TrpVal: 1.846 ± 0.044	0.611TrpTrp: 0.611 ± 0.026	0.298TrpTyr: 0.298 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.535TyrAla: 2.535 ± 0.062	0.328TyrCys: 0.328 ± 0.02	1.491TyrAsp: 1.491 ± 0.041	1.419TyrGlu: 1.419 ± 0.038	0.962TyrPhe: 0.962 ± 0.033	1.964TyrGly: 1.964 ± 0.052	0.586TyrHis: 0.586 ± 0.025	0.884TyrIle: 0.884 ± 0.036	0.499TyrLys: 0.499 ± 0.021	2.665TyrLeu: 2.665 ± 0.064	0.315TyrMet: 0.315 ± 0.022	0.505TyrAsn: 0.505 ± 0.024	1.376TyrPro: 1.376 ± 0.041	0.755TyrGln: 0.755 ± 0.03	2.416TyrArg: 2.416 ± 0.055	0.793TyrSer: 0.793 ± 0.032	1.071TyrThr: 1.071 ± 0.037	1.235TyrVal: 1.235 ± 0.039	0.53TyrTrp: 0.53 ± 0.025	0.576TyrTyr: 0.576 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2781 proteins (939222 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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