archaeon

Taxonomy: cellular organisms; Archaea; unclassified Archaea

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11629 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482SER0|A0A482SER0_9ARCH Uncharacterized protein OS=archaeon OX=1906665 GN=EON65_42685 PE=4 SV=1
MM1 pKa = 7.71FEE3 pKa = 4.06QNNVGVRR10 pKa = 11.84LRR12 pKa = 11.84NPVAAFIDD20 pKa = 4.36SIEE23 pKa = 4.3SFEE26 pKa = 4.11QADD29 pKa = 3.54MFARR33 pKa = 11.84EE34 pKa = 4.44LLSDD38 pKa = 3.83VQLIAANLEE47 pKa = 4.1NEE49 pKa = 4.15VCMDD53 pKa = 4.42EE54 pKa = 4.74ACGEE58 pKa = 4.15GDD60 pKa = 3.75GEE62 pKa = 4.72EE63 pKa = 4.01EE64 pKa = 5.44DD65 pKa = 4.06EE66 pKa = 5.13YY67 pKa = 11.58EE68 pKa = 5.35DD69 pKa = 5.73IEE71 pKa = 6.58DD72 pKa = 4.62GDD74 pKa = 4.49DD75 pKa = 3.68EE76 pKa = 5.73NIQSMPEE83 pKa = 3.87GTMNAADD90 pKa = 4.66VIDD93 pKa = 4.73EE94 pKa = 4.44STGNPDD100 pKa = 3.75LDD102 pKa = 3.61IVHH105 pKa = 6.86DD106 pKa = 4.71TIAQYY111 pKa = 11.45GEE113 pKa = 4.94DD114 pKa = 3.9SFLPPLDD121 pKa = 3.24GTAFYY126 pKa = 11.5LLICKK131 pKa = 9.71INHH134 pKa = 5.63SCLPNVVVKK143 pKa = 8.19YY144 pKa = 8.88TSSEE148 pKa = 4.03DD149 pKa = 3.53TPGQVGLVARR159 pKa = 11.84LVALRR164 pKa = 11.84EE165 pKa = 3.95ILPGEE170 pKa = 4.37EE171 pKa = 4.25LVQSYY176 pKa = 10.86VDD178 pKa = 3.64ASLPAPARR186 pKa = 11.84VKK188 pKa = 10.68LLSEE192 pKa = 4.29YY193 pKa = 10.88GFKK196 pKa = 10.42CACAKK201 pKa = 10.31CVAA204 pKa = 4.37

Molecular weight: 22.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482S6R6|A0A482S6R6_9ARCH Uncharacterized protein (Fragment) OS=archaeon OX=1906665 GN=EON65_44560 PE=4 SV=1
MM1 pKa = 7.64HH2 pKa = 7.45NNPLLPLPPHH12 pKa = 6.04IRR14 pKa = 11.84FTRR17 pKa = 11.84HH18 pKa = 5.63HH19 pKa = 6.92PLHH22 pKa = 6.74PLHH25 pKa = 6.15HH26 pKa = 6.11TRR28 pKa = 11.84RR29 pKa = 11.84LLLLLLHH36 pKa = 6.73HH37 pKa = 6.65PHH39 pKa = 6.3HH40 pKa = 6.81HH41 pKa = 5.74RR42 pKa = 11.84HH43 pKa = 4.47YY44 pKa = 10.94HH45 pKa = 5.45HH46 pKa = 6.73LQHH49 pKa = 6.96LGHH52 pKa = 7.09LHH54 pKa = 5.63PHH56 pKa = 6.19RR57 pKa = 11.84HH58 pKa = 5.87PPHH61 pKa = 6.68HH62 pKa = 6.26LHH64 pKa = 5.59SHH66 pKa = 6.16LRR68 pKa = 11.84HH69 pKa = 6.14PLLGQHH75 pKa = 6.03HH76 pKa = 6.81HH77 pKa = 6.96RR78 pKa = 11.84LHH80 pKa = 5.89SHH82 pKa = 5.61HH83 pKa = 6.8HH84 pKa = 6.2HH85 pKa = 6.5LHH87 pKa = 5.9HH88 pKa = 6.77HH89 pKa = 6.11HH90 pKa = 6.92HH91 pKa = 6.81PHH93 pKa = 6.39HH94 pKa = 6.68PHH96 pKa = 6.9LGHH99 pKa = 6.5HH100 pKa = 5.71HH101 pKa = 6.71HH102 pKa = 6.98PRR104 pKa = 11.84HH105 pKa = 6.19RR106 pKa = 11.84LPPHH110 pKa = 5.82QFHH113 pKa = 7.14HH114 pKa = 6.87LPPHH118 pKa = 5.95PHH120 pKa = 6.06HH121 pKa = 6.86HH122 pKa = 6.45HH123 pKa = 6.99LSLLQLPHH131 pKa = 7.1HH132 pKa = 6.66LRR134 pKa = 11.84PPPSS138 pKa = 3.15

Molecular weight: 16.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11629

0

11629

2917676

19

13366

250.9

28.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.037 ± 0.021

1.867 ± 0.015

5.289 ± 0.02

5.84 ± 0.03

4.052 ± 0.018

5.537 ± 0.027

2.774 ± 0.019

5.546 ± 0.019

5.496 ± 0.033

10.147 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.014

4.192 ± 0.017

4.36 ± 0.028

4.212 ± 0.022

5.024 ± 0.023

8.725 ± 0.046

5.546 ± 0.023

6.964 ± 0.023

1.094 ± 0.009

3.491 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski