[Candida] inconspicua
Taxonomy:
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5046 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4T0WXM9|A0A4T0WXM9_9ASCO t-SNARE coiled-coil homology domain-containing protein OS=[Candida] inconspicua OX=52247 GN=CANINC_003736 PE=4 SV=1
MM1 pKa = 6.62 STKK4 pKa = 10.46 SFTAEE9 pKa = 3.3 QVAEE13 pKa = 4.26 HH14 pKa = 5.91 NTRR17 pKa = 11.84 DD18 pKa = 4.55 DD19 pKa = 2.38 IWIIYY24 pKa = 8.34 NGKK27 pKa = 10.13 VYY29 pKa = 10.82 DD30 pKa = 3.59 VTQYY34 pKa = 11.52 LDD36 pKa = 3.36 EE37 pKa = 5.15 HH38 pKa = 6.75 PGGEE42 pKa = 4.59 EE43 pKa = 4.84 VILDD47 pKa = 4.08 CAGDD51 pKa = 3.86 DD52 pKa = 3.57 ATEE55 pKa = 4.08 AFDD58 pKa = 6.49 DD59 pKa = 4.1 IGHH62 pKa = 6.11 SEE64 pKa = 4.25 DD65 pKa = 3.28 AHH67 pKa = 5.87 EE68 pKa = 4.15 QLEE71 pKa = 4.28 SLLIGEE77 pKa = 5.48 LIGGVKK83 pKa = 9.73 PKK85 pKa = 10.36 SASSDD90 pKa = 3.4 SSSGSGSDD98 pKa = 3.76 PNLALYY104 pKa = 10.28 AVIIAIIAAAAYY116 pKa = 8.84 FYY118 pKa = 11.12 LNQSS122 pKa = 3.19
Molecular weight: 13.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.986
IPC_protein 3.935
Toseland 3.745
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.795
EMBOSS 3.859
Sillero 4.062
Patrickios 0.998
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|A0A4T0X312|A0A4T0X312_9ASCO Uncharacterized protein OS=[Candida] inconspicua OX=52247 GN=CANINC_002212 PE=4 SV=1
MM1 pKa = 7.41 IALIRR6 pKa = 11.84 STLATVRR13 pKa = 11.84 NNTVPSVLLSAPGMMTVSGLFVQQRR38 pKa = 11.84 FGTRR42 pKa = 11.84 GGNRR46 pKa = 11.84 GNTYY50 pKa = 10.33 QPNTLKK56 pKa = 10.67 RR57 pKa = 11.84 KK58 pKa = 9.26 RR59 pKa = 11.84 RR60 pKa = 11.84 LGFLARR66 pKa = 11.84 MKK68 pKa = 10.39 SISGRR73 pKa = 11.84 QIVKK77 pKa = 10.05 RR78 pKa = 11.84 RR79 pKa = 11.84 FEE81 pKa = 3.82 KK82 pKa = 10.88 GRR84 pKa = 11.84 WFLTHH89 pKa = 7.07
Molecular weight: 10.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.371
IPC2_protein 10.935
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.193
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.93
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5046
0
5046
2593241
14
5283
513.9
58.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.234 ± 0.031
1.215 ± 0.013
6.201 ± 0.024
6.833 ± 0.033
4.489 ± 0.023
4.826 ± 0.035
2.053 ± 0.01
7.045 ± 0.029
7.485 ± 0.036
9.5 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.026 ± 0.011
6.309 ± 0.032
4.131 ± 0.025
3.779 ± 0.036
4.071 ± 0.021
8.643 ± 0.04
5.932 ± 0.036
5.793 ± 0.029
0.967 ± 0.01
3.461 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here