Eutypa lata (strain UCR-EL1) (Grapevine dieback disease fungus) (Eutypa armeniacae)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Diatrypaceae; Eutypa; Eutypa lata

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M7TX45|M7TX45_EUTLA Dolichyl-phosphate-mannose--protein mannosyltransferase OS=Eutypa lata (strain UCR-EL1) OX=1287681 GN=UCREL1_1721 PE=3 SV=1
MM1 pKa = 6.98NAHH4 pKa = 7.47RR5 pKa = 11.84IAVDD9 pKa = 2.89IGTPKK14 pKa = 8.77QTVGLRR20 pKa = 11.84VDD22 pKa = 3.44VSSYY26 pKa = 6.96QTWAISGCGDD36 pKa = 3.62LFFNEE41 pKa = 4.78DD42 pKa = 3.68EE43 pKa = 4.32CDD45 pKa = 3.63KK46 pKa = 11.46AGTYY50 pKa = 10.39NDD52 pKa = 4.17SLSDD56 pKa = 3.18TSLYY60 pKa = 10.94VDD62 pKa = 3.53VPYY65 pKa = 10.9SEE67 pKa = 5.51SSVDD71 pKa = 3.57NDD73 pKa = 4.22DD74 pKa = 3.89GSSYY78 pKa = 11.43SLTYY82 pKa = 10.38YY83 pKa = 11.05ADD85 pKa = 4.02DD86 pKa = 3.73FTIQGGDD93 pKa = 3.24TLKK96 pKa = 11.09NITFGVFDD104 pKa = 5.16HH105 pKa = 7.15PNSDD109 pKa = 3.18QFGALGLGFGKK120 pKa = 10.59GVNSDD125 pKa = 3.03RR126 pKa = 11.84SNIVDD131 pKa = 3.61EE132 pKa = 5.25LVAQGFTQTKK142 pKa = 10.07AFSLSLGPYY151 pKa = 8.93GSEE154 pKa = 3.78EE155 pKa = 3.74ASLILGGVDD164 pKa = 3.05TKK166 pKa = 11.07KK167 pKa = 10.9FSGSLQRR174 pKa = 11.84MPIVDD179 pKa = 4.21GPEE182 pKa = 3.24FDD184 pKa = 4.39YY185 pKa = 11.46DD186 pKa = 4.46FNQTQYY192 pKa = 10.28WISIDD197 pKa = 3.44EE198 pKa = 4.36ATTNRR203 pKa = 11.84PDD205 pKa = 3.54ADD207 pKa = 3.5ATSFPSFKK215 pKa = 10.87AMQHH219 pKa = 5.44SDD221 pKa = 3.04IDD223 pKa = 4.42VSWLPWEE230 pKa = 4.15IAEE233 pKa = 4.9SVAGDD238 pKa = 3.92FGLAIAEE245 pKa = 5.1DD246 pKa = 3.74NYY248 pKa = 10.5EE249 pKa = 4.1WYY251 pKa = 9.71IVPCSAQNLTGSLDD265 pKa = 3.74LKK267 pKa = 11.09FGDD270 pKa = 4.0LALSIPYY277 pKa = 10.12GDD279 pKa = 5.32LVLQGDD285 pKa = 3.41IRR287 pKa = 11.84DD288 pKa = 3.9RR289 pKa = 11.84LVDD292 pKa = 3.52EE293 pKa = 5.77CYY295 pKa = 10.66LSVMPRR301 pKa = 11.84NATDD305 pKa = 3.17EE306 pKa = 4.37GPIYY310 pKa = 10.38WLGQNIMGHH319 pKa = 7.36LYY321 pKa = 9.88TVFDD325 pKa = 3.75QEE327 pKa = 5.24SRR329 pKa = 11.84AVWLAEE335 pKa = 3.54YY336 pKa = 9.86DD337 pKa = 3.56NCGTEE342 pKa = 4.15VVEE345 pKa = 4.13ITKK348 pKa = 10.53DD349 pKa = 3.17EE350 pKa = 4.03NAIAGINGQCGGPGIQAGGDD370 pKa = 3.61EE371 pKa = 4.61EE372 pKa = 4.28EE373 pKa = 5.1AGAGDD378 pKa = 3.74AEE380 pKa = 4.82GFGTRR385 pKa = 11.84INGPAVLLWLTSIFAGLLLGFF406 pKa = 5.07

Molecular weight: 43.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7SVI8|M7SVI8_EUTLA Putative atpase family aaa domain-containing protein 1-a protein OS=Eutypa lata (strain UCR-EL1) OX=1287681 GN=UCREL1_4440 PE=4 SV=1
MM1 pKa = 7.94PKK3 pKa = 9.44QQRR6 pKa = 11.84TLKK9 pKa = 9.11QQRR12 pKa = 11.84TLKK15 pKa = 10.15QQLTPKK21 pKa = 9.49QLKK24 pKa = 9.96RR25 pKa = 11.84RR26 pKa = 11.84IKK28 pKa = 9.55QQPTSKK34 pKa = 10.23QLKK37 pKa = 8.78RR38 pKa = 11.84QLKK41 pKa = 7.34RR42 pKa = 11.84QRR44 pKa = 11.84MPKK47 pKa = 9.25QLKK50 pKa = 7.12QQRR53 pKa = 11.84TPKK56 pKa = 9.94QQLTPKK62 pKa = 9.7QLKK65 pKa = 9.84RR66 pKa = 11.84QLKK69 pKa = 8.61QQLTPKK75 pKa = 9.73QLKK78 pKa = 9.76RR79 pKa = 11.84QLKK82 pKa = 7.34RR83 pKa = 11.84QRR85 pKa = 11.84MPKK88 pKa = 9.27QLKK91 pKa = 8.24QQQTLKK97 pKa = 10.49RR98 pKa = 11.84QRR100 pKa = 11.84TLKK103 pKa = 10.37RR104 pKa = 11.84QQTPKK109 pKa = 10.27QPKK112 pKa = 8.59

Molecular weight: 13.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11684

0

11684

5038052

66

8334

431.2

47.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.176 ± 0.022

1.13 ± 0.008

6.127 ± 0.016

6.418 ± 0.027

3.746 ± 0.014

7.6 ± 0.026

2.243 ± 0.01

4.806 ± 0.017

4.775 ± 0.021

8.708 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.009

3.669 ± 0.013

5.578 ± 0.022

3.795 ± 0.016

5.847 ± 0.023

7.46 ± 0.022

5.946 ± 0.018

6.472 ± 0.017

1.538 ± 0.009

2.814 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski