Epulopiscium sp. Nele67-Bin004

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Epulopiscium; unclassified Epulopiscium

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2474 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S9CNY1|A0A1S9CNY1_9FIRM Fido domain-containing protein OS=Epulopiscium sp. Nele67-Bin004 OX=1764962 GN=ATN35_04455 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 10.51LGTKK6 pKa = 9.05LRR8 pKa = 11.84KK9 pKa = 9.36RR10 pKa = 11.84LAFLLASSIIFASTSSINSANYY32 pKa = 8.38TRR34 pKa = 11.84QQTNSTIIDD43 pKa = 3.33VGYY46 pKa = 10.09IQINEE51 pKa = 3.84NEE53 pKa = 4.69LYY55 pKa = 10.5YY56 pKa = 11.01FPDD59 pKa = 3.32LTVNIPNNNKK69 pKa = 9.06VFGLNLFYY77 pKa = 11.13DD78 pKa = 4.59DD79 pKa = 4.52ANKK82 pKa = 10.95DD83 pKa = 3.6GVDD86 pKa = 4.53GILSLVDD93 pKa = 3.6PQIEE97 pKa = 4.28GLTYY101 pKa = 10.81TKK103 pKa = 8.11MTNLDD108 pKa = 3.76GEE110 pKa = 4.51QGSVLYY116 pKa = 10.43AFSGGIDD123 pKa = 3.25AQTAQEE129 pKa = 4.36FLRR132 pKa = 11.84ALQFDD137 pKa = 3.77ITGNTPLNIIVDD149 pKa = 3.89QNEE152 pKa = 4.11VVLPEE157 pKa = 4.48SEE159 pKa = 3.96NEE161 pKa = 3.95IYY163 pKa = 8.21FTKK166 pKa = 10.6YY167 pKa = 9.98IPEE170 pKa = 4.99DD171 pKa = 3.63DD172 pKa = 4.47PEE174 pKa = 4.13NVHH177 pKa = 6.35YY178 pKa = 10.51YY179 pKa = 10.27FYY181 pKa = 11.52VNEE184 pKa = 4.03LVDD187 pKa = 3.14WDD189 pKa = 4.09TAYY192 pKa = 10.43EE193 pKa = 3.93LAKK196 pKa = 10.13SYY198 pKa = 10.86RR199 pKa = 11.84YY200 pKa = 9.6MGMKK204 pKa = 10.22GYY206 pKa = 10.01LATITTPGEE215 pKa = 4.16DD216 pKa = 3.27QLLTSISKK224 pKa = 10.66LGGWTSGIRR233 pKa = 11.84IDD235 pKa = 3.47PTYY238 pKa = 11.11LFGEE242 pKa = 4.39NGVDD246 pKa = 4.12SDD248 pKa = 4.62TISANLTAKK257 pKa = 9.89TGIFNSGLGHH267 pKa = 6.93KK268 pKa = 10.01SNSGHH273 pKa = 5.34VQLIDD278 pKa = 3.27RR279 pKa = 11.84FYY281 pKa = 9.75WAAGPEE287 pKa = 4.33AIAQKK292 pKa = 11.1DD293 pKa = 4.06DD294 pKa = 3.46IGDD297 pKa = 3.49MTKK300 pKa = 11.01NNWGFYY306 pKa = 10.65NYY308 pKa = 10.46DD309 pKa = 3.46GTNGTTSSAIRR320 pKa = 11.84WDD322 pKa = 3.59NGYY325 pKa = 10.44EE326 pKa = 3.89PYY328 pKa = 11.0QNFTKK333 pKa = 10.78ANGEE337 pKa = 4.05NEE339 pKa = 4.2PNDD342 pKa = 3.87GGTVATKK349 pKa = 10.28EE350 pKa = 4.22YY351 pKa = 9.9IVQVNWGGTNVFAWNDD367 pKa = 3.46LAAKK371 pKa = 8.27EE372 pKa = 3.91LWIRR376 pKa = 11.84GYY378 pKa = 9.99FVEE381 pKa = 4.24MSPYY385 pKa = 10.05EE386 pKa = 4.27GGVVPGYY393 pKa = 9.88EE394 pKa = 4.09DD395 pKa = 5.21NIIEE399 pKa = 4.24TNINIIMEE407 pKa = 4.76DD408 pKa = 3.96LPVYY412 pKa = 10.72DD413 pKa = 5.27PDD415 pKa = 3.8YY416 pKa = 11.17QPTEE420 pKa = 4.29FPDD423 pKa = 3.65EE424 pKa = 4.49LPTPEE429 pKa = 4.92LDD431 pKa = 4.15GMVDD435 pKa = 4.34DD436 pKa = 4.99VFPDD440 pKa = 3.65EE441 pKa = 5.42LPTPEE446 pKa = 4.92LDD448 pKa = 4.15GMVDD452 pKa = 3.63DD453 pKa = 4.89VFPNEE458 pKa = 4.43LPTPEE463 pKa = 4.77LDD465 pKa = 3.46EE466 pKa = 5.71AGEE469 pKa = 4.14VLFPDD474 pKa = 4.23VLPTPDD480 pKa = 5.36LDD482 pKa = 4.12GLDD485 pKa = 4.78DD486 pKa = 3.94VFSDD490 pKa = 3.81VLPMPEE496 pKa = 4.62LDD498 pKa = 3.55GTIDD502 pKa = 4.36DD503 pKa = 4.53VFPDD507 pKa = 4.08VLPMPEE513 pKa = 4.58LDD515 pKa = 3.55GTIDD519 pKa = 4.36DD520 pKa = 4.53VFPDD524 pKa = 4.08VLPMPEE530 pKa = 5.2LDD532 pKa = 4.15GLDD535 pKa = 3.57DD536 pKa = 3.8VLYY539 pKa = 10.28PDD541 pKa = 4.55MLPTPEE547 pKa = 5.58LDD549 pKa = 3.76GLDD552 pKa = 3.6DD553 pKa = 3.7VLYY556 pKa = 10.19PDD558 pKa = 4.51VLPTPEE564 pKa = 5.48LDD566 pKa = 3.82GLDD569 pKa = 3.6DD570 pKa = 3.7VLYY573 pKa = 10.19PDD575 pKa = 4.51VLPTPEE581 pKa = 5.48LDD583 pKa = 3.94GLDD586 pKa = 4.04DD587 pKa = 4.06VLFPDD592 pKa = 4.85EE593 pKa = 5.42LPMPEE598 pKa = 4.61LFPYY602 pKa = 10.32VEE604 pKa = 5.18PDD606 pKa = 3.24NDD608 pKa = 3.79DD609 pKa = 5.46DD610 pKa = 5.54KK611 pKa = 12.13AEE613 pKa = 5.1DD614 pKa = 4.87DD615 pKa = 4.76NSSDD619 pKa = 4.08DD620 pKa = 3.83NTAEE624 pKa = 4.23TEE626 pKa = 4.0NNTNYY631 pKa = 10.27EE632 pKa = 4.18IKK634 pKa = 10.41DD635 pKa = 3.45KK636 pKa = 11.48NDD638 pKa = 3.54IIEE641 pKa = 3.96QDD643 pKa = 3.36EE644 pKa = 4.64PIILEE649 pKa = 4.04LEE651 pKa = 3.96ARR653 pKa = 11.84MSRR656 pKa = 11.84MLSGFEE662 pKa = 3.91IDD664 pKa = 6.0IISDD668 pKa = 3.18WDD670 pKa = 3.66FEE672 pKa = 4.56IDD674 pKa = 4.2IIEE677 pKa = 4.68TILNAKK683 pKa = 9.49VDD685 pKa = 4.32LEE687 pKa = 4.92LIDD690 pKa = 5.51AIIDD694 pKa = 3.47AGIEE698 pKa = 4.01PEE700 pKa = 3.97IVEE703 pKa = 4.2QLVCGHH709 pKa = 7.01IDD711 pKa = 3.42AEE713 pKa = 4.78QFKK716 pKa = 9.51QLCNDD721 pKa = 3.32YY722 pKa = 9.75EE723 pKa = 4.27INIIYY728 pKa = 9.33EE729 pKa = 3.88EE730 pKa = 4.74PYY732 pKa = 9.89IFGYY736 pKa = 9.84PDD738 pKa = 3.29YY739 pKa = 11.52TFMTDD744 pKa = 3.06NNLTRR749 pKa = 11.84AEE751 pKa = 4.11VVAIVSRR758 pKa = 11.84LHH760 pKa = 5.92KK761 pKa = 10.88VEE763 pKa = 3.87MDD765 pKa = 3.22EE766 pKa = 4.26TATYY770 pKa = 9.33TSSFNDD776 pKa = 3.32VYY778 pKa = 9.53PTNWHH783 pKa = 6.29ANSIGFVEE791 pKa = 4.25QQGILNGYY799 pKa = 9.17KK800 pKa = 10.43DD801 pKa = 4.39GSFKK805 pKa = 11.04PNVAITRR812 pKa = 11.84AEE814 pKa = 4.63FIALTAPLGVATEE827 pKa = 4.21PKK829 pKa = 9.97VDD831 pKa = 3.88YY832 pKa = 10.83FPFDD836 pKa = 4.61DD837 pKa = 4.13VSQSHH842 pKa = 5.79WAYY845 pKa = 9.96EE846 pKa = 4.27SLHH849 pKa = 5.84IAYY852 pKa = 9.85SQGWVAGYY860 pKa = 10.7EE861 pKa = 4.07DD862 pKa = 3.82GKK864 pKa = 10.96YY865 pKa = 9.86RR866 pKa = 11.84PDD868 pKa = 3.22EE869 pKa = 4.83AITRR873 pKa = 11.84DD874 pKa = 3.39EE875 pKa = 4.05AVAIINRR882 pKa = 11.84IIDD885 pKa = 3.57RR886 pKa = 11.84RR887 pKa = 11.84PNPMFFDD894 pKa = 4.14VIFDD898 pKa = 4.39DD899 pKa = 3.65MHH901 pKa = 6.17EE902 pKa = 4.31SHH904 pKa = 6.84WAYY907 pKa = 10.44EE908 pKa = 3.9DD909 pKa = 4.17VILSSNYY916 pKa = 10.07YY917 pKa = 9.75IRR919 pKa = 11.84LDD921 pKa = 3.41KK922 pKa = 11.29

Molecular weight: 103.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S9CQV6|A0A1S9CQV6_9FIRM Uncharacterized protein OS=Epulopiscium sp. Nele67-Bin004 OX=1764962 GN=ATN35_00150 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.49KK9 pKa = 7.5RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84EE15 pKa = 3.65HH16 pKa = 6.35GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.42NGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 9.04KK41 pKa = 9.67LTAA44 pKa = 4.2

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2474

0

2474

779308

44

1661

315.0

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.151 ± 0.051

1.08 ± 0.021

6.02 ± 0.038

7.248 ± 0.052

4.129 ± 0.034

6.052 ± 0.041

1.634 ± 0.024

9.138 ± 0.047

6.938 ± 0.055

9.022 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.023

5.617 ± 0.038

2.987 ± 0.037

3.619 ± 0.029

3.217 ± 0.031

6.174 ± 0.043

6.24 ± 0.051

6.943 ± 0.044

0.732 ± 0.014

4.333 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski