Amino acid dipepetide frequency for Epulopiscium sp. Nele67-Bin004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.304AlaAla: 3.304 ± 0.111	0.654AlaCys: 0.654 ± 0.03	3.508AlaAsp: 3.508 ± 0.081	4.036AlaGlu: 4.036 ± 0.101	2.443AlaPhe: 2.443 ± 0.059	3.933AlaGly: 3.933 ± 0.087	1.028AlaHis: 1.028 ± 0.04	5.982AlaIle: 5.982 ± 0.103	4.625AlaLys: 4.625 ± 0.1	5.614AlaLeu: 5.614 ± 0.095	1.838AlaMet: 1.838 ± 0.049	3.295AlaAsn: 3.295 ± 0.079	1.625AlaPro: 1.625 ± 0.054	2.33AlaGln: 2.33 ± 0.068	2.062AlaArg: 2.062 ± 0.052	3.693AlaSer: 3.693 ± 0.076	4.378AlaThr: 4.378 ± 0.074	4.202AlaVal: 4.202 ± 0.078	0.379AlaTrp: 0.379 ± 0.022	2.575AlaTyr: 2.575 ± 0.055	0.001AlaXaa: 0.001 ± 0.001
Cys	0.63CysAla: 0.63 ± 0.032	0.167CysCys: 0.167 ± 0.015	0.631CysAsp: 0.631 ± 0.03	0.722CysGlu: 0.722 ± 0.034	0.417CysPhe: 0.417 ± 0.025	0.844CysGly: 0.844 ± 0.037	0.227CysHis: 0.227 ± 0.017	1.039CysIle: 1.039 ± 0.037	0.802CysLys: 0.802 ± 0.032	0.856CysLeu: 0.856 ± 0.033	0.303CysMet: 0.303 ± 0.022	0.593CysAsn: 0.593 ± 0.03	0.362CysPro: 0.362 ± 0.024	0.285CysGln: 0.285 ± 0.019	0.314CysArg: 0.314 ± 0.02	0.64CysSer: 0.64 ± 0.029	0.64CysThr: 0.64 ± 0.029	0.77CysVal: 0.77 ± 0.036	0.08CysTrp: 0.08 ± 0.01	0.48CysTyr: 0.48 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	3.406AspAla: 3.406 ± 0.08	0.559AspCys: 0.559 ± 0.028	3.643AspAsp: 3.643 ± 0.091	4.885AspGlu: 4.885 ± 0.074	2.765AspPhe: 2.765 ± 0.062	3.9AspGly: 3.9 ± 0.081	0.657AspHis: 0.657 ± 0.031	6.557AspIle: 6.557 ± 0.106	4.694AspLys: 4.694 ± 0.083	4.92AspLeu: 4.92 ± 0.08	1.744AspMet: 1.744 ± 0.046	3.42AspAsn: 3.42 ± 0.078	1.468AspPro: 1.468 ± 0.051	1.249AspGln: 1.249 ± 0.044	1.812AspArg: 1.812 ± 0.056	3.039AspSer: 3.039 ± 0.073	3.93AspThr: 3.93 ± 0.08	4.172AspVal: 4.172 ± 0.08	0.499AspTrp: 0.499 ± 0.027	2.878AspTyr: 2.878 ± 0.07	0.0AspXaa: 0.0 ± 0.0
Glu	4.244GluAla: 4.244 ± 0.097	0.58GluCys: 0.58 ± 0.028	4.051GluAsp: 4.051 ± 0.092	5.822GluGlu: 5.822 ± 0.132	3.008GluPhe: 3.008 ± 0.062	3.774GluGly: 3.774 ± 0.074	1.301GluHis: 1.301 ± 0.052	6.945GluIle: 6.945 ± 0.104	5.076GluLys: 5.076 ± 0.108	6.977GluLeu: 6.977 ± 0.109	2.174GluMet: 2.174 ± 0.056	4.188GluAsn: 4.188 ± 0.074	1.873GluPro: 1.873 ± 0.079	3.504GluGln: 3.504 ± 0.089	2.409GluArg: 2.409 ± 0.053	3.239GluSer: 3.239 ± 0.067	4.048GluThr: 4.048 ± 0.102	5.492GluVal: 5.492 ± 0.097	0.622GluTrp: 0.622 ± 0.031	3.148GluTyr: 3.148 ± 0.067	0.001GluXaa: 0.001 ± 0.001
Phe	2.641PheAla: 2.641 ± 0.057	0.55PheCys: 0.55 ± 0.028	2.868PheAsp: 2.868 ± 0.053	3.27PheGlu: 3.27 ± 0.071	1.654PhePhe: 1.654 ± 0.052	2.851PheGly: 2.851 ± 0.068	0.567PheHis: 0.567 ± 0.025	3.766PheIle: 3.766 ± 0.08	2.691PheLys: 2.691 ± 0.065	3.468PheLeu: 3.468 ± 0.074	1.277PheMet: 1.277 ± 0.04	2.179PheAsn: 2.179 ± 0.053	1.043PhePro: 1.043 ± 0.035	0.869PheGln: 0.869 ± 0.039	1.265PheArg: 1.265 ± 0.044	2.946PheSer: 2.946 ± 0.064	2.205PheThr: 2.205 ± 0.055	3.186PheVal: 3.186 ± 0.069	0.311PheTrp: 0.311 ± 0.019	1.684PheTyr: 1.684 ± 0.056	0.0PheXaa: 0.0 ± 0.0
Gly	4.018GlyAla: 4.018 ± 0.093	0.743GlyCys: 0.743 ± 0.036	3.626GlyAsp: 3.626 ± 0.068	4.433GlyGlu: 4.433 ± 0.082	2.629GlyPhe: 2.629 ± 0.061	4.015GlyGly: 4.015 ± 0.097	1.034GlyHis: 1.034 ± 0.039	5.976GlyIle: 5.976 ± 0.112	4.149GlyLys: 4.149 ± 0.079	5.155GlyLeu: 5.155 ± 0.087	1.696GlyMet: 1.696 ± 0.051	2.746GlyAsn: 2.746 ± 0.055	0.943GlyPro: 0.943 ± 0.039	1.963GlyGln: 1.963 ± 0.05	1.97GlyArg: 1.97 ± 0.053	3.323GlySer: 3.323 ± 0.07	3.803GlyThr: 3.803 ± 0.081	4.936GlyVal: 4.936 ± 0.089	0.453GlyTrp: 0.453 ± 0.023	2.908GlyTyr: 2.908 ± 0.071	0.0GlyXaa: 0.0 ± 0.0
His	0.82HisAla: 0.82 ± 0.034	0.228HisCys: 0.228 ± 0.019	0.844HisAsp: 0.844 ± 0.03	0.92HisGlu: 0.92 ± 0.038	0.734HisPhe: 0.734 ± 0.031	0.934HisGly: 0.934 ± 0.035	0.388HisHis: 0.388 ± 0.029	1.549HisIle: 1.549 ± 0.057	1.213HisLys: 1.213 ± 0.042	1.569HisLeu: 1.569 ± 0.047	0.462HisMet: 0.462 ± 0.023	1.062HisAsn: 1.062 ± 0.037	0.694HisPro: 0.694 ± 0.028	0.568HisGln: 0.568 ± 0.021	0.535HisArg: 0.535 ± 0.033	1.025HisSer: 1.025 ± 0.036	1.08HisThr: 1.08 ± 0.04	0.833HisVal: 0.833 ± 0.037	0.133HisTrp: 0.133 ± 0.014	0.747HisTyr: 0.747 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.114IleAla: 6.114 ± 0.102	1.137IleCys: 1.137 ± 0.044	6.194IleAsp: 6.194 ± 0.104	7.051IleGlu: 7.051 ± 0.108	3.598IlePhe: 3.598 ± 0.082	5.47IleGly: 5.47 ± 0.105	1.25IleHis: 1.25 ± 0.038	7.871IleIle: 7.871 ± 0.133	6.177IleLys: 6.177 ± 0.106	7.845IleLeu: 7.845 ± 0.113	2.179IleMet: 2.179 ± 0.053	5.241IleAsn: 5.241 ± 0.081	3.017IlePro: 3.017 ± 0.077	2.879IleGln: 2.879 ± 0.061	2.856IleArg: 2.856 ± 0.054	6.413IleSer: 6.413 ± 0.107	5.611IleThr: 5.611 ± 0.097	6.385IleVal: 6.385 ± 0.102	0.594IleTrp: 0.594 ± 0.029	3.493IleTyr: 3.493 ± 0.078	0.0IleXaa: 0.0 ± 0.0
Lys	3.92LysAla: 3.92 ± 0.091	0.661LysCys: 0.661 ± 0.029	4.123LysAsp: 4.123 ± 0.083	5.445LysGlu: 5.445 ± 0.097	2.728LysPhe: 2.728 ± 0.065	3.481LysGly: 3.481 ± 0.082	1.25LysHis: 1.25 ± 0.043	6.095LysIle: 6.095 ± 0.091	5.256LysLys: 5.256 ± 0.1	6.592LysLeu: 6.592 ± 0.105	1.97LysMet: 1.97 ± 0.05	4.118LysAsn: 4.118 ± 0.085	1.918LysPro: 1.918 ± 0.054	3.101LysGln: 3.101 ± 0.064	2.297LysArg: 2.297 ± 0.05	4.142LysSer: 4.142 ± 0.076	3.91LysThr: 3.91 ± 0.074	4.605LysVal: 4.605 ± 0.077	0.506LysTrp: 0.506 ± 0.028	3.267LysTyr: 3.267 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	5.964LeuAla: 5.964 ± 0.083	1.083LeuCys: 1.083 ± 0.035	5.921LeuAsp: 5.921 ± 0.096	7.104LeuGlu: 7.104 ± 0.125	3.459LeuPhe: 3.459 ± 0.077	5.688LeuGly: 5.688 ± 0.091	1.406LeuHis: 1.406 ± 0.045	6.995LeuIle: 6.995 ± 0.107	6.013LeuLys: 6.013 ± 0.094	7.897LeuLeu: 7.897 ± 0.129	2.37LeuMet: 2.37 ± 0.065	4.893LeuAsn: 4.893 ± 0.091	2.973LeuPro: 2.973 ± 0.063	3.439LeuGln: 3.439 ± 0.063	2.994LeuArg: 2.994 ± 0.062	6.461LeuSer: 6.461 ± 0.101	5.437LeuThr: 5.437 ± 0.099	5.91LeuVal: 5.91 ± 0.099	0.581LeuTrp: 0.581 ± 0.026	3.635LeuTyr: 3.635 ± 0.075	0.001LeuXaa: 0.001 ± 0.001
Met	1.839MetAla: 1.839 ± 0.051	0.316MetCys: 0.316 ± 0.022	1.396MetAsp: 1.396 ± 0.045	1.65MetGlu: 1.65 ± 0.043	1.193MetPhe: 1.193 ± 0.043	1.673MetGly: 1.673 ± 0.051	0.507MetHis: 0.507 ± 0.024	2.201MetIle: 2.201 ± 0.054	1.864MetLys: 1.864 ± 0.045	2.724MetLeu: 2.724 ± 0.057	0.747MetMet: 0.747 ± 0.035	1.39MetAsn: 1.39 ± 0.039	1.125MetPro: 1.125 ± 0.035	1.209MetGln: 1.209 ± 0.039	0.996MetArg: 0.996 ± 0.037	1.787MetSer: 1.787 ± 0.045	1.612MetThr: 1.612 ± 0.051	1.673MetVal: 1.673 ± 0.055	0.199MetTrp: 0.199 ± 0.016	1.165MetTyr: 1.165 ± 0.04	0.001MetXaa: 0.001 ± 0.001
Asn	2.921AsnAla: 2.921 ± 0.062	0.568AsnCys: 0.568 ± 0.028	2.862AsnAsp: 2.862 ± 0.079	3.35AsnGlu: 3.35 ± 0.078	2.326AsnPhe: 2.326 ± 0.052	3.22AsnGly: 3.22 ± 0.074	0.82AsnHis: 0.82 ± 0.035	5.899AsnIle: 5.899 ± 0.094	4.251AsnLys: 4.251 ± 0.081	5.375AsnLeu: 5.375 ± 0.113	1.64AsnMet: 1.64 ± 0.048	3.658AsnAsn: 3.658 ± 0.075	2.022AsnPro: 2.022 ± 0.053	1.897AsnGln: 1.897 ± 0.053	1.825AsnArg: 1.825 ± 0.051	3.399AsnSer: 3.399 ± 0.08	3.607AsnThr: 3.607 ± 0.071	3.611AsnVal: 3.611 ± 0.073	0.465AsnTrp: 0.465 ± 0.026	2.451AsnTyr: 2.451 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	1.686ProAla: 1.686 ± 0.05	0.26ProCys: 0.26 ± 0.019	1.959ProAsp: 1.959 ± 0.061	2.383ProGlu: 2.383 ± 0.101	1.283ProPhe: 1.283 ± 0.046	1.101ProGly: 1.101 ± 0.036	0.613ProHis: 0.613 ± 0.027	2.841ProIle: 2.841 ± 0.062	2.048ProLys: 2.048 ± 0.06	2.541ProLeu: 2.541 ± 0.056	0.742ProMet: 0.742 ± 0.035	1.841ProAsn: 1.841 ± 0.058	0.883ProPro: 0.883 ± 0.06	1.145ProGln: 1.145 ± 0.042	0.833ProArg: 0.833 ± 0.038	1.838ProSer: 1.838 ± 0.051	2.205ProThr: 2.205 ± 0.067	2.074ProVal: 2.074 ± 0.055	0.231ProTrp: 0.231 ± 0.018	1.361ProTyr: 1.361 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.338GlnAla: 2.338 ± 0.062	0.308GlnCys: 0.308 ± 0.021	1.831GlnAsp: 1.831 ± 0.049	2.483GlnGlu: 2.483 ± 0.066	1.435GlnPhe: 1.435 ± 0.051	1.879GlnGly: 1.879 ± 0.048	0.616GlnHis: 0.616 ± 0.029	3.382GlnIle: 3.382 ± 0.063	2.56GlnLys: 2.56 ± 0.063	3.547GlnLeu: 3.547 ± 0.075	1.048GlnMet: 1.048 ± 0.033	2.29GlnAsn: 2.29 ± 0.056	0.944GlnPro: 0.944 ± 0.037	1.902GlnGln: 1.902 ± 0.063	1.08GlnArg: 1.08 ± 0.036	1.932GlnSer: 1.932 ± 0.054	2.261GlnThr: 2.261 ± 0.061	2.523GlnVal: 2.523 ± 0.06	0.259GlnTrp: 0.259 ± 0.02	1.573GlnTyr: 1.573 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.045ArgAla: 2.045 ± 0.047	0.355ArgCys: 0.355 ± 0.023	1.925ArgAsp: 1.925 ± 0.05	2.665ArgGlu: 2.665 ± 0.064	1.3ArgPhe: 1.3 ± 0.041	2.044ArgGly: 2.044 ± 0.043	0.61ArgHis: 0.61 ± 0.031	2.606ArgIle: 2.606 ± 0.065	2.215ArgLys: 2.215 ± 0.061	3.012ArgLeu: 3.012 ± 0.064	0.948ArgMet: 0.948 ± 0.032	1.745ArgAsn: 1.745 ± 0.049	1.006ArgPro: 1.006 ± 0.041	1.388ArgGln: 1.388 ± 0.045	1.279ArgArg: 1.279 ± 0.047	1.518ArgSer: 1.518 ± 0.044	1.726ArgThr: 1.726 ± 0.046	2.085ArgVal: 2.085 ± 0.055	0.259ArgTrp: 0.259 ± 0.017	1.436ArgTyr: 1.436 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	3.782SerAla: 3.782 ± 0.071	0.615SerCys: 0.615 ± 0.027	3.539SerAsp: 3.539 ± 0.072	3.807SerGlu: 3.807 ± 0.081	2.515SerPhe: 2.515 ± 0.052	4.149SerGly: 4.149 ± 0.079	1.122SerHis: 1.122 ± 0.036	5.643SerIle: 5.643 ± 0.095	4.204SerLys: 4.204 ± 0.079	5.615SerLeu: 5.615 ± 0.085	1.636SerMet: 1.636 ± 0.048	3.42SerAsn: 3.42 ± 0.071	1.818SerPro: 1.818 ± 0.057	2.238SerGln: 2.238 ± 0.065	2.04SerArg: 2.04 ± 0.048	4.367SerSer: 4.367 ± 0.121	4.038SerThr: 4.038 ± 0.091	4.011SerVal: 4.011 ± 0.078	0.454SerTrp: 0.454 ± 0.026	2.725SerTyr: 2.725 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	3.924ThrAla: 3.924 ± 0.08	0.526ThrCys: 0.526 ± 0.029	3.474ThrAsp: 3.474 ± 0.077	3.784ThrGlu: 3.784 ± 0.087	2.628ThrPhe: 2.628 ± 0.058	3.884ThrGly: 3.884 ± 0.076	1.143ThrHis: 1.143 ± 0.045	5.909ThrIle: 5.909 ± 0.086	4.174ThrLys: 4.174 ± 0.075	5.858ThrLeu: 5.858 ± 0.09	1.431ThrMet: 1.431 ± 0.046	3.593ThrAsn: 3.593 ± 0.072	2.407ThrPro: 2.407 ± 0.076	2.24ThrGln: 2.24 ± 0.053	1.763ThrArg: 1.763 ± 0.038	4.046ThrSer: 4.046 ± 0.082	4.63ThrThr: 4.63 ± 0.116	4.122ThrVal: 4.122 ± 0.074	0.359ThrTrp: 0.359 ± 0.021	2.506ThrTyr: 2.506 ± 0.057	0.001ThrXaa: 0.001 ± 0.001
Val	5.074ValAla: 5.074 ± 0.092	0.901ValCys: 0.901 ± 0.036	4.619ValAsp: 4.619 ± 0.08	5.216ValGlu: 5.216 ± 0.079	2.796ValPhe: 2.796 ± 0.077	4.717ValGly: 4.717 ± 0.089	0.966ValHis: 0.966 ± 0.033	5.673ValIle: 5.673 ± 0.082	3.912ValLys: 3.912 ± 0.078	6.063ValLeu: 6.063 ± 0.1	1.805ValMet: 1.805 ± 0.049	3.19ValAsn: 3.19 ± 0.061	2.278ValPro: 2.278 ± 0.052	2.276ValGln: 2.276 ± 0.058	2.176ValArg: 2.176 ± 0.051	4.847ValSer: 4.847 ± 0.084	4.093ValThr: 4.093 ± 0.077	5.559ValVal: 5.559 ± 0.089	0.475ValTrp: 0.475 ± 0.024	2.788ValTyr: 2.788 ± 0.056	0.0ValXaa: 0.0 ± 0.0
Trp	0.445TrpAla: 0.445 ± 0.026	0.076TrpCys: 0.076 ± 0.01	0.493TrpAsp: 0.493 ± 0.025	0.562TrpGlu: 0.562 ± 0.03	0.384TrpPhe: 0.384 ± 0.023	0.48TrpGly: 0.48 ± 0.025	0.136TrpHis: 0.136 ± 0.012	0.676TrpIle: 0.676 ± 0.036	0.416TrpLys: 0.416 ± 0.022	0.73TrpLeu: 0.73 ± 0.029	0.153TrpMet: 0.153 ± 0.013	0.379TrpAsn: 0.379 ± 0.02	0.133TrpPro: 0.133 ± 0.013	0.316TrpGln: 0.316 ± 0.021	0.25TrpArg: 0.25 ± 0.016	0.399TrpSer: 0.399 ± 0.024	0.337TrpThr: 0.337 ± 0.02	0.447TrpVal: 0.447 ± 0.026	0.089TrpTrp: 0.089 ± 0.011	0.418TrpTyr: 0.418 ± 0.029	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.392TyrAla: 2.392 ± 0.06	0.515TyrCys: 0.515 ± 0.026	2.688TyrAsp: 2.688 ± 0.063	2.887TyrGlu: 2.887 ± 0.056	1.992TyrPhe: 1.992 ± 0.048	2.483TyrGly: 2.483 ± 0.064	0.699TyrHis: 0.699 ± 0.031	3.771TyrIle: 3.771 ± 0.061	3.045TyrLys: 3.045 ± 0.07	3.866TyrLeu: 3.866 ± 0.071	1.105TyrMet: 1.105 ± 0.037	2.924TyrAsn: 2.924 ± 0.062	1.333TyrPro: 1.333 ± 0.046	1.392TyrGln: 1.392 ± 0.047	1.412TyrArg: 1.412 ± 0.04	2.683TyrSer: 2.683 ± 0.059	2.85TyrThr: 2.85 ± 0.069	2.829TyrVal: 2.829 ± 0.069	0.371TyrTrp: 0.371 ± 0.024	2.086TyrTyr: 2.086 ± 0.061	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.003XaaPhe: 0.003 ± 0.002	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2474 proteins (779308 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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