Salmonella phage 118970_sal3
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C9IHS2|A0A1C9IHS2_9CAUD Tail sheath protein OS=Salmonella phage 118970_sal3 OX=1813771 GN=118970sal3_00015 PE=3 SV=1
MM1 pKa = 7.62 YY2 pKa = 10.05 YY3 pKa = 10.28 QFDD6 pKa = 3.82 FTVKK10 pKa = 10.8 YY11 pKa = 10.15 EE12 pKa = 3.88 ITEE15 pKa = 4.01 TDD17 pKa = 2.86 TRR19 pKa = 11.84 QQDD22 pKa = 4.07 DD23 pKa = 4.3 LDD25 pKa = 4.25 GLPDD29 pKa = 4.72 LKK31 pKa = 10.26 TLSIDD36 pKa = 3.07 VDD38 pKa = 4.72 FIEE41 pKa = 5.7 PGTGPDD47 pKa = 3.74 GDD49 pKa = 4.36 IEE51 pKa = 4.41 HH52 pKa = 6.56 HH53 pKa = 5.86 TEE55 pKa = 3.39 ITFQEE60 pKa = 4.26
Molecular weight: 6.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.024
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.503
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.427
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.846
Patrickios 2.994
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A1C9II65|A0A1C9II65_9CAUD Tail sheath protein OS=Salmonella phage 118970_sal3 OX=1813771 GN=118970sal3_00016 PE=4 SV=1
MM1 pKa = 7.28 IRR3 pKa = 11.84 NIFKK7 pKa = 10.73 RR8 pKa = 11.84 FTSQRR13 pKa = 11.84 FHH15 pKa = 7.26 CPRR18 pKa = 11.84 PGQWYY23 pKa = 7.33 STPEE27 pKa = 4.29 GYY29 pKa = 10.24 VLRR32 pKa = 11.84 ISLVDD37 pKa = 3.65 RR38 pKa = 11.84 EE39 pKa = 4.39 CQKK42 pKa = 10.54 VVCEE46 pKa = 3.98 PLGRR50 pKa = 11.84 NYY52 pKa = 9.88 RR53 pKa = 11.84 VNMPLIAFRR62 pKa = 11.84 SGKK65 pKa = 8.95 NMKK68 pKa = 9.95 HH69 pKa = 6.22 LGGAAA74 pKa = 3.13
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.692
IPC_protein 10.452
Toseland 10.496
ProMoST 10.218
Dawson 10.643
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.862
Grimsley 10.701
Solomon 10.73
Lehninger 10.687
Nozaki 10.526
DTASelect 10.35
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.643
IPC_peptide 10.73
IPC2_peptide 9.619
IPC2.peptide.svr19 8.418
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
111
0
111
19496
37
642
175.6
19.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.74 ± 0.275
1.298 ± 0.118
5.847 ± 0.223
6.073 ± 0.214
3.478 ± 0.126
7.663 ± 0.252
1.816 ± 0.118
5.278 ± 0.199
5.155 ± 0.235
8.55 ± 0.198
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.611 ± 0.123
4.139 ± 0.215
4.462 ± 0.163
4.17 ± 0.149
6.299 ± 0.275
6.247 ± 0.223
5.935 ± 0.22
6.74 ± 0.157
1.611 ± 0.12
2.888 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here