Pseudomonas phage vB_PaeP_E220

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Hollowayvirus; unclassified Hollowayvirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8I3B4|A0A2K8I3B4_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_E220 OX=2034343 GN=vBPaePE220_00062 PE=3 SV=1
MM1 pKa = 7.28ATEE4 pKa = 4.28QILDD8 pKa = 3.41IDD10 pKa = 3.99RR11 pKa = 11.84TPLEE15 pKa = 4.29VGAMYY20 pKa = 10.58CCVNLMLATDD30 pKa = 3.58GRR32 pKa = 11.84EE33 pKa = 3.37ICRR36 pKa = 11.84DD37 pKa = 3.14YY38 pKa = 11.35GALVRR43 pKa = 11.84YY44 pKa = 10.02VGVTSGEE51 pKa = 4.27YY52 pKa = 9.48KK53 pKa = 10.12RR54 pKa = 11.84HH55 pKa = 4.78VFADD59 pKa = 3.24ADD61 pKa = 3.63TWEE64 pKa = 4.27EE65 pKa = 3.93TRR67 pKa = 11.84VYY69 pKa = 11.07ADD71 pKa = 3.73QLLKK75 pKa = 10.48QQAPAIDD82 pKa = 4.49PATQGWADD90 pKa = 3.47LL91 pKa = 4.05

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8IBK8|A0A2K8IBK8_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_E220 OX=2034343 GN=vBPaePE220_00007 PE=4 SV=1
MM1 pKa = 7.08KK2 pKa = 10.05QAPSTMQNEE11 pKa = 4.62TLGHH15 pKa = 6.32IRR17 pKa = 11.84AFWAEE22 pKa = 3.93KK23 pKa = 10.48GYY25 pKa = 11.18APTVAEE31 pKa = 4.49LAAKK35 pKa = 10.42AGVRR39 pKa = 11.84QFAIQQRR46 pKa = 11.84LTALEE51 pKa = 3.97NKK53 pKa = 10.21GYY55 pKa = 9.67IQRR58 pKa = 11.84DD59 pKa = 3.59PKK61 pKa = 9.84VARR64 pKa = 11.84SIRR67 pKa = 11.84PLL69 pKa = 3.42

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

19900

30

3851

297.0

32.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.774 ± 0.535

0.784 ± 0.178

6.166 ± 0.228

6.302 ± 0.29

2.935 ± 0.153

8.025 ± 0.262

2.0 ± 0.194

4.106 ± 0.244

3.834 ± 0.298

8.156 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.143

2.955 ± 0.149

5.407 ± 0.322

5.286 ± 0.234

7.382 ± 0.318

5.221 ± 0.239

5.412 ± 0.26

6.829 ± 0.264

1.477 ± 0.148

2.588 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski