Amino acid dipepetide frequency for Pseudomonas phage vB_PaeP_E220

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.735AlaAla: 16.735 ± 1.442
1.055AlaCys: 1.055 ± 0.397
8.694AlaAsp: 8.694 ± 0.759
10.051AlaGlu: 10.051 ± 0.868
3.015AlaPhe: 3.015 ± 0.305
10.252AlaGly: 10.252 ± 0.806
1.96AlaHis: 1.96 ± 0.309
5.377AlaIle: 5.377 ± 0.502
5.578AlaLys: 5.578 ± 0.759
10.553AlaLeu: 10.553 ± 0.935
3.216AlaMet: 3.216 ± 0.368
3.116AlaAsn: 3.116 ± 0.361
6.533AlaPro: 6.533 ± 0.588
7.287AlaGln: 7.287 ± 0.983
9.247AlaArg: 9.247 ± 0.717
5.779AlaSer: 5.779 ± 0.732
5.93AlaThr: 5.93 ± 0.636
8.443AlaVal: 8.443 ± 0.614
1.96AlaTrp: 1.96 ± 0.329
2.965AlaTyr: 2.965 ± 0.476
0.0AlaXaa: 0.0 ± 0.0
Cys
0.754CysAla: 0.754 ± 0.289
0.251CysCys: 0.251 ± 0.131
0.553CysAsp: 0.553 ± 0.24
0.553CysGlu: 0.553 ± 0.178
0.101CysPhe: 0.101 ± 0.084
0.804CysGly: 0.804 ± 0.236
0.251CysHis: 0.251 ± 0.137
0.352CysIle: 0.352 ± 0.145
0.251CysLys: 0.251 ± 0.118
0.452CysLeu: 0.452 ± 0.169
0.101CysMet: 0.101 ± 0.074
0.101CysAsn: 0.101 ± 0.074
0.352CysPro: 0.352 ± 0.158
0.503CysGln: 0.503 ± 0.196
0.503CysArg: 0.503 ± 0.216
0.653CysSer: 0.653 ± 0.26
0.352CysThr: 0.352 ± 0.15
0.402CysVal: 0.402 ± 0.153
0.151CysTrp: 0.151 ± 0.098
0.402CysTyr: 0.402 ± 0.188
0.0CysXaa: 0.0 ± 0.0
Asp
9.548AspAla: 9.548 ± 0.779
0.402AspCys: 0.402 ± 0.165
4.071AspAsp: 4.071 ± 0.506
3.669AspGlu: 3.669 ± 0.476
1.96AspPhe: 1.96 ± 0.352
5.427AspGly: 5.427 ± 0.583
1.005AspHis: 1.005 ± 0.26
2.663AspIle: 2.663 ± 0.348
1.608AspLys: 1.608 ± 0.307
5.126AspLeu: 5.126 ± 0.517
1.709AspMet: 1.709 ± 0.291
1.307AspAsn: 1.307 ± 0.297
2.915AspPro: 2.915 ± 0.447
3.266AspGln: 3.266 ± 0.382
3.719AspArg: 3.719 ± 0.425
3.116AspSer: 3.116 ± 0.375
3.266AspThr: 3.266 ± 0.472
4.372AspVal: 4.372 ± 0.508
0.804AspTrp: 0.804 ± 0.184
1.709AspTyr: 1.709 ± 0.361
0.0AspXaa: 0.0 ± 0.0
Glu
8.091GluAla: 8.091 ± 0.894
0.503GluCys: 0.503 ± 0.204
2.764GluAsp: 2.764 ± 0.388
3.417GluGlu: 3.417 ± 0.526
2.161GluPhe: 2.161 ± 0.574
4.523GluGly: 4.523 ± 0.62
1.307GluHis: 1.307 ± 0.288
2.814GluIle: 2.814 ± 0.542
2.764GluLys: 2.764 ± 0.426
6.081GluLeu: 6.081 ± 0.582
1.307GluMet: 1.307 ± 0.257
1.407GluAsn: 1.407 ± 0.18
2.915GluPro: 2.915 ± 0.551
4.774GluGln: 4.774 ± 0.55
5.377GluArg: 5.377 ± 0.543
3.719GluSer: 3.719 ± 0.462
2.462GluThr: 2.462 ± 0.437
3.92GluVal: 3.92 ± 0.466
0.905GluTrp: 0.905 ± 0.237
1.809GluTyr: 1.809 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
3.317PheAla: 3.317 ± 0.414
0.151PheCys: 0.151 ± 0.107
2.01PheAsp: 2.01 ± 0.425
2.261PheGlu: 2.261 ± 0.39
0.653PhePhe: 0.653 ± 0.126
2.613PheGly: 2.613 ± 0.463
0.503PheHis: 0.503 ± 0.176
0.905PheIle: 0.905 ± 0.248
0.905PheLys: 0.905 ± 0.219
2.261PheLeu: 2.261 ± 0.391
0.452PheMet: 0.452 ± 0.134
0.653PheAsn: 0.653 ± 0.158
1.859PhePro: 1.859 ± 0.291
1.508PheGln: 1.508 ± 0.236
1.759PheArg: 1.759 ± 0.275
1.91PheSer: 1.91 ± 0.265
1.508PheThr: 1.508 ± 0.348
2.563PheVal: 2.563 ± 0.347
0.503PheTrp: 0.503 ± 0.229
1.055PheTyr: 1.055 ± 0.362
0.0PheXaa: 0.0 ± 0.0
Gly
7.99GlyAla: 7.99 ± 0.701
0.653GlyCys: 0.653 ± 0.281
4.674GlyAsp: 4.674 ± 0.478
5.829GlyGlu: 5.829 ± 0.608
3.216GlyPhe: 3.216 ± 0.564
6.835GlyGly: 6.835 ± 0.521
0.854GlyHis: 0.854 ± 0.171
3.518GlyIle: 3.518 ± 0.465
3.116GlyLys: 3.116 ± 0.425
5.729GlyLeu: 5.729 ± 0.624
2.161GlyMet: 2.161 ± 0.326
2.563GlyAsn: 2.563 ± 0.361
2.965GlyPro: 2.965 ± 0.449
4.975GlyGln: 4.975 ± 0.489
6.081GlyArg: 6.081 ± 0.625
4.724GlySer: 4.724 ± 0.428
4.573GlyThr: 4.573 ± 0.689
6.03GlyVal: 6.03 ± 0.531
1.558GlyTrp: 1.558 ± 0.267
2.211GlyTyr: 2.211 ± 0.338
0.0GlyXaa: 0.0 ± 0.0
His
2.211HisAla: 2.211 ± 0.321
0.251HisCys: 0.251 ± 0.121
0.955HisAsp: 0.955 ± 0.275
1.357HisGlu: 1.357 ± 0.235
0.905HisPhe: 0.905 ± 0.306
1.658HisGly: 1.658 ± 0.341
0.754HisHis: 0.754 ± 0.237
0.754HisIle: 0.754 ± 0.214
0.251HisLys: 0.251 ± 0.079
2.211HisLeu: 2.211 ± 0.522
0.402HisMet: 0.402 ± 0.119
0.653HisAsn: 0.653 ± 0.226
0.854HisPro: 0.854 ± 0.225
1.106HisGln: 1.106 ± 0.222
2.06HisArg: 2.06 ± 0.301
1.005HisSer: 1.005 ± 0.318
1.005HisThr: 1.005 ± 0.259
0.955HisVal: 0.955 ± 0.187
0.201HisTrp: 0.201 ± 0.106
0.452HisTyr: 0.452 ± 0.176
0.0HisXaa: 0.0 ± 0.0
Ile
4.975IleAla: 4.975 ± 0.516
0.553IleCys: 0.553 ± 0.187
3.266IleAsp: 3.266 ± 0.328
3.266IleGlu: 3.266 ± 0.46
0.452IlePhe: 0.452 ± 0.177
3.518IleGly: 3.518 ± 0.404
0.854IleHis: 0.854 ± 0.254
1.357IleIle: 1.357 ± 0.34
2.161IleLys: 2.161 ± 0.339
2.513IleLeu: 2.513 ± 0.265
0.653IleMet: 0.653 ± 0.15
1.809IleAsn: 1.809 ± 0.331
2.513IlePro: 2.513 ± 0.281
1.256IleGln: 1.256 ± 0.282
3.015IleArg: 3.015 ± 0.332
2.111IleSer: 2.111 ± 0.315
2.965IleThr: 2.965 ± 0.501
2.211IleVal: 2.211 ± 0.342
0.553IleTrp: 0.553 ± 0.155
1.055IleTyr: 1.055 ± 0.323
0.0IleXaa: 0.0 ± 0.0
Lys
5.427LysAla: 5.427 ± 0.902
0.251LysCys: 0.251 ± 0.131
2.312LysAsp: 2.312 ± 0.383
1.91LysGlu: 1.91 ± 0.345
1.005LysPhe: 1.005 ± 0.213
2.864LysGly: 2.864 ± 0.418
0.804LysHis: 0.804 ± 0.213
1.206LysIle: 1.206 ± 0.28
1.96LysLys: 1.96 ± 0.355
3.468LysLeu: 3.468 ± 0.443
1.055LysMet: 1.055 ± 0.269
0.854LysAsn: 0.854 ± 0.241
2.915LysPro: 2.915 ± 0.405
2.01LysGln: 2.01 ± 0.26
2.513LysArg: 2.513 ± 0.403
1.809LysSer: 1.809 ± 0.326
1.759LysThr: 1.759 ± 0.337
2.915LysVal: 2.915 ± 0.324
0.302LysTrp: 0.302 ± 0.112
1.005LysTyr: 1.005 ± 0.201
0.0LysXaa: 0.0 ± 0.0
Leu
11.558LeuAla: 11.558 ± 0.877
0.452LeuCys: 0.452 ± 0.234
5.126LeuAsp: 5.126 ± 0.553
5.176LeuGlu: 5.176 ± 0.485
2.412LeuPhe: 2.412 ± 0.374
5.779LeuGly: 5.779 ± 0.518
2.06LeuHis: 2.06 ± 0.304
2.462LeuIle: 2.462 ± 0.474
2.965LeuLys: 2.965 ± 0.347
6.684LeuLeu: 6.684 ± 0.839
2.161LeuMet: 2.161 ± 0.357
3.065LeuAsn: 3.065 ± 0.475
4.774LeuPro: 4.774 ± 0.578
3.015LeuGln: 3.015 ± 0.342
6.231LeuArg: 6.231 ± 0.746
4.523LeuSer: 4.523 ± 0.509
4.975LeuThr: 4.975 ± 0.641
5.98LeuVal: 5.98 ± 0.753
0.804LeuTrp: 0.804 ± 0.163
1.357LeuTyr: 1.357 ± 0.359
0.0LeuXaa: 0.0 ± 0.0
Met
3.367MetAla: 3.367 ± 0.474
0.151MetCys: 0.151 ± 0.09
1.206MetAsp: 1.206 ± 0.275
0.754MetGlu: 0.754 ± 0.195
0.402MetPhe: 0.402 ± 0.156
2.01MetGly: 2.01 ± 0.42
0.503MetHis: 0.503 ± 0.145
0.804MetIle: 0.804 ± 0.196
1.156MetLys: 1.156 ± 0.223
1.859MetLeu: 1.859 ± 0.3
0.352MetMet: 0.352 ± 0.119
0.754MetAsn: 0.754 ± 0.187
1.91MetPro: 1.91 ± 0.298
1.357MetGln: 1.357 ± 0.282
1.91MetArg: 1.91 ± 0.408
1.558MetSer: 1.558 ± 0.286
1.658MetThr: 1.658 ± 0.239
1.005MetVal: 1.005 ± 0.234
0.302MetTrp: 0.302 ± 0.122
0.603MetTyr: 0.603 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
3.719AsnAla: 3.719 ± 0.529
0.352AsnCys: 0.352 ± 0.158
1.508AsnAsp: 1.508 ± 0.331
1.508AsnGlu: 1.508 ± 0.28
0.754AsnPhe: 0.754 ± 0.201
2.161AsnGly: 2.161 ± 0.358
0.452AsnHis: 0.452 ± 0.164
1.256AsnIle: 1.256 ± 0.341
1.106AsnLys: 1.106 ± 0.206
2.714AsnLeu: 2.714 ± 0.39
0.905AsnMet: 0.905 ± 0.24
0.653AsnAsn: 0.653 ± 0.233
1.91AsnPro: 1.91 ± 0.394
1.508AsnGln: 1.508 ± 0.313
2.211AsnArg: 2.211 ± 0.34
1.96AsnSer: 1.96 ± 0.264
1.859AsnThr: 1.859 ± 0.278
1.558AsnVal: 1.558 ± 0.277
0.754AsnTrp: 0.754 ± 0.216
0.704AsnTyr: 0.704 ± 0.222
0.0AsnXaa: 0.0 ± 0.0
Pro
7.689ProAla: 7.689 ± 0.681
0.251ProCys: 0.251 ± 0.166
4.422ProAsp: 4.422 ± 0.549
3.669ProGlu: 3.669 ± 0.432
1.206ProPhe: 1.206 ± 0.261
3.97ProGly: 3.97 ± 0.701
0.653ProHis: 0.653 ± 0.212
2.563ProIle: 2.563 ± 0.554
2.06ProLys: 2.06 ± 0.332
3.417ProLeu: 3.417 ± 0.516
1.357ProMet: 1.357 ± 0.251
1.608ProAsn: 1.608 ± 0.386
3.266ProPro: 3.266 ± 1.01
2.211ProGln: 2.211 ± 0.436
2.915ProArg: 2.915 ± 0.383
2.965ProSer: 2.965 ± 0.426
3.518ProThr: 3.518 ± 0.362
3.92ProVal: 3.92 ± 0.48
0.804ProTrp: 0.804 ± 0.201
1.608ProTyr: 1.608 ± 0.356
0.0ProXaa: 0.0 ± 0.0
Gln
8.392GlnAla: 8.392 ± 0.71
0.251GlnCys: 0.251 ± 0.109
2.01GlnAsp: 2.01 ± 0.306
2.965GlnGlu: 2.965 ± 0.369
1.809GlnPhe: 1.809 ± 0.258
4.171GlnGly: 4.171 ± 0.54
1.809GlnHis: 1.809 ± 0.293
3.065GlnIle: 3.065 ± 0.651
1.106GlnLys: 1.106 ± 0.271
3.015GlnLeu: 3.015 ± 0.431
1.206GlnMet: 1.206 ± 0.317
1.508GlnAsn: 1.508 ± 0.407
2.915GlnPro: 2.915 ± 0.311
4.623GlnGln: 4.623 ± 0.733
3.669GlnArg: 3.669 ± 0.492
2.01GlnSer: 2.01 ± 0.329
2.01GlnThr: 2.01 ± 0.305
3.719GlnVal: 3.719 ± 0.414
0.754GlnTrp: 0.754 ± 0.223
1.859GlnTyr: 1.859 ± 0.251
0.0GlnXaa: 0.0 ± 0.0
Arg
7.89ArgAla: 7.89 ± 0.731
0.603ArgCys: 0.603 ± 0.207
4.322ArgAsp: 4.322 ± 0.566
4.674ArgGlu: 4.674 ± 0.802
3.065ArgPhe: 3.065 ± 0.393
5.226ArgGly: 5.226 ± 0.701
1.457ArgHis: 1.457 ± 0.384
3.568ArgIle: 3.568 ± 0.462
3.266ArgLys: 3.266 ± 0.42
6.633ArgLeu: 6.633 ± 0.565
1.558ArgMet: 1.558 ± 0.25
2.261ArgAsn: 2.261 ± 0.321
3.468ArgPro: 3.468 ± 0.572
4.121ArgGln: 4.121 ± 0.554
6.181ArgArg: 6.181 ± 0.695
3.266ArgSer: 3.266 ± 0.776
3.618ArgThr: 3.618 ± 0.546
5.478ArgVal: 5.478 ± 0.482
1.055ArgTrp: 1.055 ± 0.297
2.06ArgTyr: 2.06 ± 0.403
0.0ArgXaa: 0.0 ± 0.0
Ser
6.432SerAla: 6.432 ± 0.657
0.101SerCys: 0.101 ± 0.073
3.065SerAsp: 3.065 ± 0.489
3.015SerGlu: 3.015 ± 0.443
1.457SerPhe: 1.457 ± 0.243
5.076SerGly: 5.076 ± 0.749
1.055SerHis: 1.055 ± 0.312
1.759SerIle: 1.759 ± 0.231
2.261SerLys: 2.261 ± 0.286
4.221SerLeu: 4.221 ± 0.479
1.508SerMet: 1.508 ± 0.265
1.859SerAsn: 1.859 ± 0.328
3.216SerPro: 3.216 ± 0.457
2.563SerGln: 2.563 ± 0.288
3.166SerArg: 3.166 ± 0.37
2.663SerSer: 2.663 ± 0.33
3.216SerThr: 3.216 ± 0.451
3.266SerVal: 3.266 ± 0.334
0.905SerTrp: 0.905 ± 0.191
1.407SerTyr: 1.407 ± 0.375
0.0SerXaa: 0.0 ± 0.0
Thr
7.237ThrAla: 7.237 ± 0.854
0.352ThrCys: 0.352 ± 0.17
3.317ThrAsp: 3.317 ± 0.332
2.462ThrGlu: 2.462 ± 0.408
1.859ThrPhe: 1.859 ± 0.369
5.226ThrGly: 5.226 ± 0.492
1.005ThrHis: 1.005 ± 0.236
2.663ThrIle: 2.663 ± 0.509
1.759ThrLys: 1.759 ± 0.271
4.02ThrLeu: 4.02 ± 0.485
1.005ThrMet: 1.005 ± 0.211
1.658ThrAsn: 1.658 ± 0.229
3.518ThrPro: 3.518 ± 0.45
2.06ThrGln: 2.06 ± 0.315
3.317ThrArg: 3.317 ± 0.438
2.814ThrSer: 2.814 ± 0.339
3.116ThrThr: 3.116 ± 0.566
4.372ThrVal: 4.372 ± 0.536
0.905ThrTrp: 0.905 ± 0.169
1.457ThrTyr: 1.457 ± 0.212
0.0ThrXaa: 0.0 ± 0.0
Val
8.292ValAla: 8.292 ± 0.677
0.704ValCys: 0.704 ± 0.238
5.025ValAsp: 5.025 ± 0.553
4.422ValGlu: 4.422 ± 0.642
1.558ValPhe: 1.558 ± 0.389
4.925ValGly: 4.925 ± 0.524
1.96ValHis: 1.96 ± 0.368
2.915ValIle: 2.915 ± 0.43
2.563ValLys: 2.563 ± 0.384
6.382ValLeu: 6.382 ± 0.517
1.709ValMet: 1.709 ± 0.23
1.658ValAsn: 1.658 ± 0.254
3.568ValPro: 3.568 ± 0.574
3.116ValGln: 3.116 ± 0.426
5.528ValArg: 5.528 ± 0.584
3.468ValSer: 3.468 ± 0.439
3.819ValThr: 3.819 ± 0.47
4.573ValVal: 4.573 ± 0.537
0.754ValTrp: 0.754 ± 0.256
1.357ValTyr: 1.357 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
1.759TrpAla: 1.759 ± 0.29
0.251TrpCys: 0.251 ± 0.147
0.804TrpAsp: 0.804 ± 0.241
0.653TrpGlu: 0.653 ± 0.147
0.251TrpPhe: 0.251 ± 0.124
1.055TrpGly: 1.055 ± 0.247
0.352TrpHis: 0.352 ± 0.103
0.251TrpIle: 0.251 ± 0.125
0.653TrpLys: 0.653 ± 0.156
1.91TrpLeu: 1.91 ± 0.354
0.352TrpMet: 0.352 ± 0.125
0.653TrpAsn: 0.653 ± 0.151
0.704TrpPro: 0.704 ± 0.16
0.402TrpGln: 0.402 ± 0.122
1.608TrpArg: 1.608 ± 0.285
0.503TrpSer: 0.503 ± 0.209
0.854TrpThr: 0.854 ± 0.221
0.955TrpVal: 0.955 ± 0.208
0.302TrpTrp: 0.302 ± 0.145
0.503TrpTyr: 0.503 ± 0.194
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.362TyrAla: 2.362 ± 0.384
0.302TyrCys: 0.302 ± 0.116
1.558TyrAsp: 1.558 ± 0.249
1.407TyrGlu: 1.407 ± 0.296
1.106TyrPhe: 1.106 ± 0.276
2.161TyrGly: 2.161 ± 0.357
0.402TyrHis: 0.402 ± 0.166
0.804TyrIle: 0.804 ± 0.164
0.854TyrLys: 0.854 ± 0.204
2.312TyrLeu: 2.312 ± 0.301
0.402TyrMet: 0.402 ± 0.125
1.407TyrAsn: 1.407 ± 0.325
1.005TyrPro: 1.005 ± 0.253
1.206TyrGln: 1.206 ± 0.279
2.814TyrArg: 2.814 ± 0.36
1.658TyrSer: 1.658 ± 0.37
1.658TyrThr: 1.658 ± 0.285
1.658TyrVal: 1.658 ± 0.374
0.503TyrTrp: 0.503 ± 0.189
0.302TyrTyr: 0.302 ± 0.095
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (19900 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski