Lactobacillus phage Lbab1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; unclassified Herelleviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9V4L6|A0A7G9V4L6_9CAUD Endonuclease OS=Lactobacillus phage Lbab1 OX=2769358 PE=4 SV=1
MM1 pKa = 7.35VDD3 pKa = 4.2KK4 pKa = 11.34DD5 pKa = 4.0EE6 pKa = 4.74LCIAKK11 pKa = 10.09SIYY14 pKa = 10.55NKK16 pKa = 7.69TLKK19 pKa = 10.82GEE21 pKa = 4.01QQFYY25 pKa = 10.87LGNFNKK31 pKa = 10.15EE32 pKa = 4.9KK33 pKa = 10.86NDD35 pKa = 2.86IWNDD39 pKa = 2.87GWQQGIAFVTGVVSAMVNHH58 pKa = 7.32PYY60 pKa = 10.61GYY62 pKa = 10.89DD63 pKa = 4.31DD64 pKa = 5.55INDD67 pKa = 3.8LNLAIINEE75 pKa = 4.39LNWEE79 pKa = 4.24QTNSSYY85 pKa = 11.47EE86 pKa = 4.14EE87 pKa = 4.36YY88 pKa = 9.97PVSDD92 pKa = 3.59DD93 pKa = 4.25TITNSQYY100 pKa = 11.22YY101 pKa = 10.48DD102 pKa = 3.5GFLLAINTEE111 pKa = 3.79IGILKK116 pKa = 9.74QAMSEE121 pKa = 4.08FFDD124 pKa = 4.15YY125 pKa = 11.41EE126 pKa = 4.24DD127 pKa = 3.49VSEE130 pKa = 4.48VNN132 pKa = 3.47

Molecular weight: 15.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9V4G8|A0A7G9V4G8_9CAUD Uncharacterized protein OS=Lactobacillus phage Lbab1 OX=2769358 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84KK3 pKa = 8.96RR4 pKa = 11.84DD5 pKa = 3.46RR6 pKa = 11.84TNKK9 pKa = 9.51KK10 pKa = 8.2KK11 pKa = 10.69QKK13 pKa = 9.46IEE15 pKa = 3.86SRR17 pKa = 11.84RR18 pKa = 11.84FNHH21 pKa = 6.68NIYY24 pKa = 9.11LTAPFKK30 pKa = 11.04GRR32 pKa = 11.84STRR35 pKa = 11.84ITAIKK40 pKa = 9.86ISLRR44 pKa = 11.84RR45 pKa = 11.84AIKK48 pKa = 10.39DD49 pKa = 3.31RR50 pKa = 11.84LKK52 pKa = 10.89QLDD55 pKa = 3.84KK56 pKa = 11.02EE57 pKa = 4.22IEE59 pKa = 4.02HH60 pKa = 7.39DD61 pKa = 3.36KK62 pKa = 11.36GIYY65 pKa = 9.07

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

202

0

202

41852

30

1687

207.2

23.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.396 ± 0.26

0.729 ± 0.088

6.865 ± 0.169

5.68 ± 0.2

3.548 ± 0.119

6.188 ± 0.212

1.749 ± 0.106

6.494 ± 0.136

8.724 ± 0.304

8.212 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.102

6.026 ± 0.159

3.226 ± 0.129

3.727 ± 0.171

3.543 ± 0.152

7.624 ± 0.271

6.064 ± 0.282

6.384 ± 0.143

1.166 ± 0.069

5.204 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski