Amino acid dipepetide frequency for Lactobacillus phage Lbab1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.871AlaAla: 3.871 ± 0.512
0.239AlaCys: 0.239 ± 0.077
3.728AlaAsp: 3.728 ± 0.402
3.369AlaGlu: 3.369 ± 0.297
2.366AlaPhe: 2.366 ± 0.244
3.536AlaGly: 3.536 ± 0.315
1.123AlaHis: 1.123 ± 0.153
4.659AlaIle: 4.659 ± 0.318
6.38AlaLys: 6.38 ± 0.389
4.683AlaLeu: 4.683 ± 0.378
1.816AlaMet: 1.816 ± 0.205
4.468AlaAsn: 4.468 ± 0.322
1.912AlaPro: 1.912 ± 0.3
2.413AlaGln: 2.413 ± 0.321
2.103AlaArg: 2.103 ± 0.246
4.803AlaSer: 4.803 ± 0.527
4.588AlaThr: 4.588 ± 0.44
3.871AlaVal: 3.871 ± 0.329
0.86AlaTrp: 0.86 ± 0.13
3.178AlaTyr: 3.178 ± 0.312
0.0AlaXaa: 0.0 ± 0.0
Cys
0.406CysAla: 0.406 ± 0.1
0.048CysCys: 0.048 ± 0.031
0.43CysAsp: 0.43 ± 0.096
0.263CysGlu: 0.263 ± 0.073
0.191CysPhe: 0.191 ± 0.065
0.884CysGly: 0.884 ± 0.202
0.167CysHis: 0.167 ± 0.064
0.335CysIle: 0.335 ± 0.086
0.669CysLys: 0.669 ± 0.16
0.526CysLeu: 0.526 ± 0.117
0.287CysMet: 0.287 ± 0.089
0.287CysAsn: 0.287 ± 0.093
0.621CysPro: 0.621 ± 0.142
0.167CysGln: 0.167 ± 0.064
0.215CysArg: 0.215 ± 0.077
0.502CysSer: 0.502 ± 0.117
0.335CysThr: 0.335 ± 0.106
0.263CysVal: 0.263 ± 0.081
0.167CysTrp: 0.167 ± 0.066
0.526CysTyr: 0.526 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
4.564AspAla: 4.564 ± 0.351
0.358AspCys: 0.358 ± 0.11
4.444AspAsp: 4.444 ± 0.417
3.728AspGlu: 3.728 ± 0.344
2.7AspPhe: 2.7 ± 0.226
4.588AspGly: 4.588 ± 0.488
0.98AspHis: 0.98 ± 0.129
5.376AspIle: 5.376 ± 0.347
6.475AspLys: 6.475 ± 0.512
5.567AspLeu: 5.567 ± 0.376
1.696AspMet: 1.696 ± 0.207
4.014AspAsn: 4.014 ± 0.309
2.485AspPro: 2.485 ± 0.23
1.816AspGln: 1.816 ± 0.249
2.27AspArg: 2.27 ± 0.237
4.994AspSer: 4.994 ± 0.391
4.492AspThr: 4.492 ± 0.291
3.56AspVal: 3.56 ± 0.278
0.908AspTrp: 0.908 ± 0.146
3.632AspTyr: 3.632 ± 0.323
0.0AspXaa: 0.0 ± 0.0
Glu
3.393GluAla: 3.393 ± 0.359
0.597GluCys: 0.597 ± 0.119
4.779GluAsp: 4.779 ± 0.394
4.086GluGlu: 4.086 ± 0.448
1.744GluPhe: 1.744 ± 0.22
2.557GluGly: 2.557 ± 0.29
1.434GluHis: 1.434 ± 0.189
3.56GluIle: 3.56 ± 0.312
5.089GluLys: 5.089 ± 0.498
4.803GluLeu: 4.803 ± 0.372
0.932GluMet: 0.932 ± 0.156
3.082GluAsn: 3.082 ± 0.273
1.625GluPro: 1.625 ± 0.198
2.652GluGln: 2.652 ± 0.266
1.72GluArg: 1.72 ± 0.203
4.134GluSer: 4.134 ± 0.293
2.772GluThr: 2.772 ± 0.332
3.775GluVal: 3.775 ± 0.348
0.645GluTrp: 0.645 ± 0.107
3.417GluTyr: 3.417 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
1.84PheAla: 1.84 ± 0.227
0.191PheCys: 0.191 ± 0.065
2.222PheAsp: 2.222 ± 0.204
1.625PheGlu: 1.625 ± 0.176
1.147PhePhe: 1.147 ± 0.157
2.318PheGly: 2.318 ± 0.229
0.454PheHis: 0.454 ± 0.104
2.294PheIle: 2.294 ± 0.259
3.178PheLys: 3.178 ± 0.325
2.294PheLeu: 2.294 ± 0.219
1.075PheMet: 1.075 ± 0.156
2.509PheAsn: 2.509 ± 0.229
1.362PhePro: 1.362 ± 0.191
0.908PheGln: 0.908 ± 0.164
1.171PheArg: 1.171 ± 0.2
3.584PheSer: 3.584 ± 0.33
2.581PheThr: 2.581 ± 0.243
2.604PheVal: 2.604 ± 0.285
0.335PheTrp: 0.335 ± 0.104
1.792PheTyr: 1.792 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
3.202GlyAla: 3.202 ± 0.361
0.382GlyCys: 0.382 ± 0.081
3.919GlyAsp: 3.919 ± 0.344
2.796GlyGlu: 2.796 ± 0.266
2.294GlyPhe: 2.294 ± 0.223
3.847GlyGly: 3.847 ± 0.511
1.266GlyHis: 1.266 ± 0.218
4.851GlyIle: 4.851 ± 0.365
5.83GlyLys: 5.83 ± 0.471
4.803GlyLeu: 4.803 ± 0.394
1.816GlyMet: 1.816 ± 0.251
3.895GlyAsn: 3.895 ± 0.368
0.502GlyPro: 0.502 ± 0.088
1.84GlyGln: 1.84 ± 0.248
2.342GlyArg: 2.342 ± 0.277
5.089GlySer: 5.089 ± 0.428
4.683GlyThr: 4.683 ± 0.558
4.014GlyVal: 4.014 ± 0.284
0.836GlyTrp: 0.836 ± 0.141
3.68GlyTyr: 3.68 ± 0.345
0.0GlyXaa: 0.0 ± 0.0
His
0.812HisAla: 0.812 ± 0.153
0.239HisCys: 0.239 ± 0.082
1.266HisAsp: 1.266 ± 0.21
0.86HisGlu: 0.86 ± 0.165
0.573HisPhe: 0.573 ± 0.12
0.932HisGly: 0.932 ± 0.154
0.335HisHis: 0.335 ± 0.094
1.171HisIle: 1.171 ± 0.167
1.505HisLys: 1.505 ± 0.242
1.577HisLeu: 1.577 ± 0.207
0.311HisMet: 0.311 ± 0.081
1.29HisAsn: 1.29 ± 0.192
0.669HisPro: 0.669 ± 0.139
0.741HisGln: 0.741 ± 0.147
0.597HisArg: 0.597 ± 0.106
1.075HisSer: 1.075 ± 0.211
1.171HisThr: 1.171 ± 0.144
1.147HisVal: 1.147 ± 0.191
0.167HisTrp: 0.167 ± 0.061
1.051HisTyr: 1.051 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
4.397IleAla: 4.397 ± 0.365
0.717IleCys: 0.717 ± 0.146
4.946IleAsp: 4.946 ± 0.373
3.584IleGlu: 3.584 ± 0.296
1.696IlePhe: 1.696 ± 0.18
3.966IleGly: 3.966 ± 0.337
0.812IleHis: 0.812 ± 0.128
3.369IleIle: 3.369 ± 0.308
6.905IleLys: 6.905 ± 0.548
4.779IleLeu: 4.779 ± 0.355
1.529IleMet: 1.529 ± 0.166
4.158IleAsn: 4.158 ± 0.259
2.604IlePro: 2.604 ± 0.271
2.294IleGln: 2.294 ± 0.272
2.604IleArg: 2.604 ± 0.262
5.352IleSer: 5.352 ± 0.357
3.823IleThr: 3.823 ± 0.364
3.728IleVal: 3.728 ± 0.27
0.741IleTrp: 0.741 ± 0.14
2.939IleTyr: 2.939 ± 0.289
0.0IleXaa: 0.0 ± 0.0
Lys
5.902LysAla: 5.902 ± 0.364
0.597LysCys: 0.597 ± 0.141
6.595LysAsp: 6.595 ± 0.527
6.762LysGlu: 6.762 ± 0.594
3.082LysPhe: 3.082 ± 0.311
5.089LysGly: 5.089 ± 0.34
1.84LysHis: 1.84 ± 0.268
5.376LysIle: 5.376 ± 0.39
6.356LysLys: 6.356 ± 0.549
7.909LysLeu: 7.909 ± 0.653
2.103LysMet: 2.103 ± 0.244
4.851LysAsn: 4.851 ± 0.423
2.413LysPro: 2.413 ± 0.255
4.086LysGln: 4.086 ± 0.337
3.489LysArg: 3.489 ± 0.339
5.926LysSer: 5.926 ± 0.46
4.277LysThr: 4.277 ± 0.323
5.95LysVal: 5.95 ± 0.388
0.597LysTrp: 0.597 ± 0.147
4.038LysTyr: 4.038 ± 0.399
0.0LysXaa: 0.0 ± 0.0
Leu
6.236LeuAla: 6.236 ± 0.41
0.669LeuCys: 0.669 ± 0.117
6.236LeuAsp: 6.236 ± 0.392
4.803LeuGlu: 4.803 ± 0.49
2.963LeuPhe: 2.963 ± 0.287
5.185LeuGly: 5.185 ± 0.345
1.314LeuHis: 1.314 ± 0.207
4.683LeuIle: 4.683 ± 0.368
6.571LeuLys: 6.571 ± 0.413
6.523LeuLeu: 6.523 ± 0.446
1.983LeuMet: 1.983 ± 0.231
4.851LeuAsn: 4.851 ± 0.324
3.154LeuPro: 3.154 ± 0.29
2.891LeuGln: 2.891 ± 0.281
3.035LeuArg: 3.035 ± 0.264
6.332LeuSer: 6.332 ± 0.393
4.97LeuThr: 4.97 ± 0.318
5.305LeuVal: 5.305 ± 0.357
0.812LeuTrp: 0.812 ± 0.143
3.608LeuTyr: 3.608 ± 0.359
0.0LeuXaa: 0.0 ± 0.0
Met
1.673MetAla: 1.673 ± 0.237
0.119MetCys: 0.119 ± 0.051
1.601MetAsp: 1.601 ± 0.213
1.529MetGlu: 1.529 ± 0.191
1.027MetPhe: 1.027 ± 0.157
1.625MetGly: 1.625 ± 0.238
0.335MetHis: 0.335 ± 0.081
1.41MetIle: 1.41 ± 0.17
2.079MetLys: 2.079 ± 0.226
2.676MetLeu: 2.676 ± 0.287
0.502MetMet: 0.502 ± 0.111
1.29MetAsn: 1.29 ± 0.168
0.717MetPro: 0.717 ± 0.131
1.123MetGln: 1.123 ± 0.151
0.789MetArg: 0.789 ± 0.114
1.959MetSer: 1.959 ± 0.264
1.314MetThr: 1.314 ± 0.167
1.386MetVal: 1.386 ± 0.173
0.191MetTrp: 0.191 ± 0.065
1.147MetTyr: 1.147 ± 0.19
0.0MetXaa: 0.0 ± 0.0
Asn
3.728AsnAla: 3.728 ± 0.368
0.478AsnCys: 0.478 ± 0.102
3.775AsnAsp: 3.775 ± 0.276
2.915AsnGlu: 2.915 ± 0.313
2.031AsnPhe: 2.031 ± 0.25
4.182AsnGly: 4.182 ± 0.31
1.362AsnHis: 1.362 ± 0.175
3.56AsnIle: 3.56 ± 0.309
5.615AsnLys: 5.615 ± 0.373
4.564AsnLeu: 4.564 ± 0.325
1.481AsnMet: 1.481 ± 0.214
3.68AsnAsn: 3.68 ± 0.422
2.437AsnPro: 2.437 ± 0.241
2.748AsnGln: 2.748 ± 0.286
2.031AsnArg: 2.031 ± 0.211
4.588AsnSer: 4.588 ± 0.418
3.202AsnThr: 3.202 ± 0.282
3.751AsnVal: 3.751 ± 0.31
0.597AsnTrp: 0.597 ± 0.128
3.512AsnTyr: 3.512 ± 0.276
0.0AsnXaa: 0.0 ± 0.0
Pro
1.792ProAla: 1.792 ± 0.244
0.215ProCys: 0.215 ± 0.091
2.748ProAsp: 2.748 ± 0.285
2.342ProGlu: 2.342 ± 0.268
1.29ProPhe: 1.29 ± 0.183
1.171ProGly: 1.171 ± 0.178
0.669ProHis: 0.669 ± 0.134
2.342ProIle: 2.342 ± 0.294
2.628ProLys: 2.628 ± 0.29
2.342ProLeu: 2.342 ± 0.236
0.789ProMet: 0.789 ± 0.133
1.864ProAsn: 1.864 ± 0.228
0.43ProPro: 0.43 ± 0.125
0.98ProGln: 0.98 ± 0.23
1.147ProArg: 1.147 ± 0.19
2.676ProSer: 2.676 ± 0.29
2.031ProThr: 2.031 ± 0.267
2.437ProVal: 2.437 ± 0.32
0.502ProTrp: 0.502 ± 0.107
1.864ProTyr: 1.864 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
3.274GlnAla: 3.274 ± 0.408
0.119GlnCys: 0.119 ± 0.07
2.318GlnAsp: 2.318 ± 0.264
2.509GlnGlu: 2.509 ± 0.232
1.123GlnPhe: 1.123 ± 0.173
2.581GlnGly: 2.581 ± 0.268
0.311GlnHis: 0.311 ± 0.08
2.413GlnIle: 2.413 ± 0.225
2.604GlnLys: 2.604 ± 0.279
3.512GlnLeu: 3.512 ± 0.286
0.956GlnMet: 0.956 ± 0.17
1.601GlnAsn: 1.601 ± 0.179
1.123GlnPro: 1.123 ± 0.258
1.912GlnGln: 1.912 ± 0.333
1.338GlnArg: 1.338 ± 0.197
2.318GlnSer: 2.318 ± 0.331
1.864GlnThr: 1.864 ± 0.26
3.345GlnVal: 3.345 ± 0.279
0.43GlnTrp: 0.43 ± 0.105
1.625GlnTyr: 1.625 ± 0.185
0.0GlnXaa: 0.0 ± 0.0
Arg
2.342ArgAla: 2.342 ± 0.244
0.43ArgCys: 0.43 ± 0.134
1.888ArgAsp: 1.888 ± 0.183
2.413ArgGlu: 2.413 ± 0.262
1.912ArgPhe: 1.912 ± 0.231
2.127ArgGly: 2.127 ± 0.223
0.693ArgHis: 0.693 ± 0.163
2.15ArgIle: 2.15 ± 0.208
2.796ArgLys: 2.796 ± 0.279
3.274ArgLeu: 3.274 ± 0.31
0.956ArgMet: 0.956 ± 0.151
2.007ArgAsn: 2.007 ± 0.222
1.147ArgPro: 1.147 ± 0.137
1.458ArgGln: 1.458 ± 0.219
1.696ArgArg: 1.696 ± 0.253
2.055ArgSer: 2.055 ± 0.225
1.505ArgThr: 1.505 ± 0.185
2.222ArgVal: 2.222 ± 0.257
0.311ArgTrp: 0.311 ± 0.08
2.055ArgTyr: 2.055 ± 0.212
0.0ArgXaa: 0.0 ± 0.0
Ser
4.325SerAla: 4.325 ± 0.626
0.43SerCys: 0.43 ± 0.106
4.922SerAsp: 4.922 ± 0.348
3.441SerGlu: 3.441 ± 0.313
2.676SerPhe: 2.676 ± 0.245
5.806SerGly: 5.806 ± 0.644
1.458SerHis: 1.458 ± 0.216
5.257SerIle: 5.257 ± 0.396
7.359SerLys: 7.359 ± 0.558
6.236SerLeu: 6.236 ± 0.379
1.72SerMet: 1.72 ± 0.182
4.803SerAsn: 4.803 ± 0.402
2.127SerPro: 2.127 ± 0.244
2.652SerGln: 2.652 ± 0.294
2.676SerArg: 2.676 ± 0.218
6.308SerSer: 6.308 ± 0.586
4.564SerThr: 4.564 ± 0.458
3.966SerVal: 3.966 ± 0.352
1.219SerTrp: 1.219 ± 0.181
4.301SerTyr: 4.301 ± 0.263
0.0SerXaa: 0.0 ± 0.0
Thr
3.321ThrAla: 3.321 ± 0.372
0.478ThrCys: 0.478 ± 0.125
4.134ThrAsp: 4.134 ± 0.389
2.939ThrGlu: 2.939 ± 0.274
2.342ThrPhe: 2.342 ± 0.293
4.038ThrGly: 4.038 ± 0.456
0.836ThrHis: 0.836 ± 0.166
4.874ThrIle: 4.874 ± 0.34
4.731ThrLys: 4.731 ± 0.323
5.591ThrLeu: 5.591 ± 0.443
1.243ThrMet: 1.243 ± 0.165
3.584ThrAsn: 3.584 ± 0.335
2.413ThrPro: 2.413 ± 0.297
1.935ThrGln: 1.935 ± 0.328
1.912ThrArg: 1.912 ± 0.214
3.895ThrSer: 3.895 ± 0.347
3.632ThrThr: 3.632 ± 0.533
4.516ThrVal: 4.516 ± 0.37
0.908ThrTrp: 0.908 ± 0.168
3.321ThrTyr: 3.321 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
4.42ValAla: 4.42 ± 0.364
0.311ValCys: 0.311 ± 0.098
4.564ValAsp: 4.564 ± 0.322
3.226ValGlu: 3.226 ± 0.322
2.198ValPhe: 2.198 ± 0.223
3.632ValGly: 3.632 ± 0.294
1.027ValHis: 1.027 ± 0.162
3.489ValIle: 3.489 ± 0.263
5.376ValLys: 5.376 ± 0.419
5.233ValLeu: 5.233 ± 0.407
1.338ValMet: 1.338 ± 0.18
4.014ValAsn: 4.014 ± 0.34
2.413ValPro: 2.413 ± 0.265
2.007ValGln: 2.007 ± 0.256
1.959ValArg: 1.959 ± 0.233
6.021ValSer: 6.021 ± 0.409
4.588ValThr: 4.588 ± 0.368
4.373ValVal: 4.373 ± 0.372
0.669ValTrp: 0.669 ± 0.113
2.987ValTyr: 2.987 ± 0.315
0.0ValXaa: 0.0 ± 0.0
Trp
0.645TrpAla: 0.645 ± 0.111
0.215TrpCys: 0.215 ± 0.065
0.86TrpAsp: 0.86 ± 0.152
0.812TrpGlu: 0.812 ± 0.14
0.526TrpPhe: 0.526 ± 0.113
0.693TrpGly: 0.693 ± 0.136
0.072TrpHis: 0.072 ± 0.04
0.526TrpIle: 0.526 ± 0.108
0.741TrpLys: 0.741 ± 0.14
1.171TrpLeu: 1.171 ± 0.184
0.263TrpMet: 0.263 ± 0.072
0.669TrpAsn: 0.669 ± 0.145
0.143TrpPro: 0.143 ± 0.053
0.43TrpGln: 0.43 ± 0.115
0.358TrpArg: 0.358 ± 0.092
1.004TrpSer: 1.004 ± 0.166
0.812TrpThr: 0.812 ± 0.153
0.669TrpVal: 0.669 ± 0.124
0.143TrpTrp: 0.143 ± 0.084
0.908TrpTyr: 0.908 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.823TyrAla: 3.823 ± 0.342
0.454TyrCys: 0.454 ± 0.109
3.274TyrAsp: 3.274 ± 0.23
2.796TyrGlu: 2.796 ± 0.294
1.601TyrPhe: 1.601 ± 0.21
3.13TyrGly: 3.13 ± 0.284
1.004TyrHis: 1.004 ± 0.168
3.25TyrIle: 3.25 ± 0.295
4.349TyrLys: 4.349 ± 0.372
4.062TyrLeu: 4.062 ± 0.414
1.673TyrMet: 1.673 ± 0.194
3.345TyrAsn: 3.345 ± 0.276
1.959TyrPro: 1.959 ± 0.257
2.222TyrGln: 2.222 ± 0.241
1.983TyrArg: 1.983 ± 0.25
3.632TyrSer: 3.632 ± 0.284
3.441TyrThr: 3.441 ± 0.322
2.963TyrVal: 2.963 ± 0.313
0.621TyrTrp: 0.621 ± 0.121
2.461TyrTyr: 2.461 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 202 proteins (41852 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski