Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; Shewanella amazonensis

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3638 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1S9W0|A1S9W0_SHEAM Uncharacterized protein OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) OX=326297 GN=Sama_2964 PE=4 SV=1
MM1 pKa = 7.71SYY3 pKa = 10.62CKK5 pKa = 10.47SLVLSASLLLCCPAVLADD23 pKa = 4.1TPTSGVGDD31 pKa = 4.01EE32 pKa = 4.77MSLSVPLQTLLEE44 pKa = 4.25SSGRR48 pKa = 11.84DD49 pKa = 3.28NLIDD53 pKa = 4.11LFQIGVQNEE62 pKa = 3.86AMVAQSGDD70 pKa = 3.95SNSLIVTQIGVANQALVRR88 pKa = 11.84QLGTDD93 pKa = 3.48NEE95 pKa = 4.22VDD97 pKa = 3.82LFQAGNHH104 pKa = 5.51NSAEE108 pKa = 3.96ITQIGDD114 pKa = 3.31NNLVQLKK121 pKa = 9.91QLGSANFSIQQIGDD135 pKa = 3.65GASIAVTQYY144 pKa = 11.43

Molecular weight: 15.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1S1M2|A1S1M2_SHEAM ABC-type nitrate/sulfonate/bicarbonate transport system protein OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) OX=326297 GN=Sama_0066 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3638

0

3638

1259955

34

4214

346.3

38.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.21 ± 0.043

1.038 ± 0.013

5.678 ± 0.041

6.07 ± 0.036

3.938 ± 0.027

7.638 ± 0.043

2.234 ± 0.019

5.296 ± 0.035

4.488 ± 0.031

11.228 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.662 ± 0.021

3.563 ± 0.03

4.275 ± 0.027

4.193 ± 0.027

5.236 ± 0.043

6.267 ± 0.029

5.01 ± 0.036

6.896 ± 0.04

1.278 ± 0.016

2.802 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski