Sporomusaceae bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; unclassified Sporomusaceae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3820 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A402BVE1|A0A402BVE1_9FIRM Uncharacterized protein OS=Sporomusaceae bacterium OX=1917525 GN=SPFL3102_03204 PE=4 SV=1
MM1 pKa = 7.06MMSMYY6 pKa = 10.57SGVSGLKK13 pKa = 10.01AQQTKK18 pKa = 11.07LNVIGNNIANVSTTGYY34 pKa = 10.46KK35 pKa = 9.45YY36 pKa = 9.09QTVSFSDD43 pKa = 4.09LLSQTLSGATAANTATNQGGTNAKK67 pKa = 10.18QIGLGVGVSATTTVMTTGSTQSTGNSSDD95 pKa = 3.41VSISGDD101 pKa = 3.08GFFIVKK107 pKa = 9.96GGSSGEE113 pKa = 4.1YY114 pKa = 9.53QFTRR118 pKa = 11.84AGNFGVDD125 pKa = 3.12ANGNLTVGGLTVCGWQDD142 pKa = 3.26YY143 pKa = 10.72GGAAQADD150 pKa = 3.85GTYY153 pKa = 9.96TYY155 pKa = 8.22DD156 pKa = 3.26TQKK159 pKa = 9.63TVEE162 pKa = 4.73PINLFSDD169 pKa = 4.14SYY171 pKa = 11.47NGNKK175 pKa = 9.73QIIAPEE181 pKa = 3.94ATTAATLTGNLTSSATASGTALNDD205 pKa = 2.86IGTGFSFDD213 pKa = 3.49TTDD216 pKa = 3.38ADD218 pKa = 5.02AITTMTVYY226 pKa = 10.57DD227 pKa = 4.09AQGNSYY233 pKa = 10.67DD234 pKa = 3.57VQVNFKK240 pKa = 9.91KK241 pKa = 10.71CYY243 pKa = 10.41VDD245 pKa = 3.45TTDD248 pKa = 4.17ADD250 pKa = 3.87NPVTSWYY257 pKa = 9.51WEE259 pKa = 3.97ADD261 pKa = 3.46SSDD264 pKa = 3.57TTLSNASGYY273 pKa = 8.34ITFDD277 pKa = 3.25STGKK281 pKa = 10.23IIPVTDD287 pKa = 3.25STADD291 pKa = 3.39YY292 pKa = 7.5TTSPKK297 pKa = 9.0ITIAGSDD304 pKa = 3.39SVTPFEE310 pKa = 5.2ISLDD314 pKa = 3.49LTGISTYY321 pKa = 10.12TSSSSNGVSVSQIDD335 pKa = 4.42GYY337 pKa = 11.39EE338 pKa = 4.02SGEE341 pKa = 4.08LQDD344 pKa = 4.0FTIGSDD350 pKa = 3.81GVITGVYY357 pKa = 10.44SNGQTQPLGMIALATFANAAGLEE380 pKa = 4.24KK381 pKa = 10.14IGNNLYY387 pKa = 8.78VTTANSGAFTGGVPAGSKK405 pKa = 9.29GTGSLSSGTLEE416 pKa = 3.98MSNVDD421 pKa = 3.58LAEE424 pKa = 4.09QFSEE428 pKa = 4.07MMITQRR434 pKa = 11.84AYY436 pKa = 9.88QANSKK441 pKa = 10.22IISTSDD447 pKa = 2.89EE448 pKa = 4.03MLQTLINMVKK458 pKa = 10.47

Molecular weight: 47.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A402BRZ4|A0A402BRZ4_9FIRM Uncharacterized protein OS=Sporomusaceae bacterium OX=1917525 GN=SPFL3102_01922 PE=4 SV=1
MM1 pKa = 7.98CYY3 pKa = 10.39LNKK6 pKa = 9.99QSEE9 pKa = 4.45NLHH12 pKa = 5.98TNHH15 pKa = 5.94QSRR18 pKa = 11.84WIARR22 pKa = 11.84SITVFQQSRR31 pKa = 11.84SYY33 pKa = 9.14QVRR36 pKa = 11.84WLNLSAVRR44 pKa = 11.84TLTWWKK50 pKa = 10.45VAGLLILFGVAFIQLIKK67 pKa = 10.77VLNLDD72 pKa = 3.32HH73 pKa = 6.95HH74 pKa = 6.64AA75 pKa = 4.62

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3818

2

3820

1142366

29

2748

299.0

33.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.94 ± 0.043

1.168 ± 0.019

4.913 ± 0.029

6.076 ± 0.041

3.882 ± 0.028

7.303 ± 0.039

2.003 ± 0.019

7.812 ± 0.037

5.74 ± 0.034

10.002 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.02

4.181 ± 0.032

3.828 ± 0.025

4.13 ± 0.03

4.443 ± 0.027

5.849 ± 0.029

5.51 ± 0.042

7.357 ± 0.034

0.95 ± 0.015

3.264 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski