Wenling triplecross lizardfish paramyxovirus
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GNG5|A0A2P1GNG5_9MONO RNA-directed RNA polymerase L OS=Wenling triplecross lizardfish paramyxovirus OX=2116451 PE=3 SV=1
MM1 pKa = 7.32 KK2 pKa = 10.29 RR3 pKa = 11.84 VLDD6 pKa = 3.71 WVHH9 pKa = 6.34 QKK11 pKa = 7.91 MARR14 pKa = 11.84 KK15 pKa = 9.49 GLDD18 pKa = 3.89 PINPTIKK25 pKa = 10.09 VGRR28 pKa = 11.84 DD29 pKa = 3.2 GTEE32 pKa = 3.43 VDD34 pKa = 3.44 QGLAIYY40 pKa = 9.6 IARR43 pKa = 11.84 VTVSILAIILLTVLLVTPTVTGLFQVFDD71 pKa = 4.07 SSASEE76 pKa = 3.87 QSHH79 pKa = 7.37 PIMDD83 pKa = 5.11 LLNDD87 pKa = 3.66 EE88 pKa = 4.7 VLPPLRR94 pKa = 11.84 NTHH97 pKa = 6.59 ALLEE101 pKa = 4.5 EE102 pKa = 4.36 DD103 pKa = 3.25 TAPRR107 pKa = 11.84 VRR109 pKa = 11.84 SIQTAVEE116 pKa = 3.69 LRR118 pKa = 11.84 IPGDD122 pKa = 3.61 LKK124 pKa = 11.22 SATQQILHH132 pKa = 7.31 AIKK135 pKa = 9.08 DD136 pKa = 3.8 TCVGGDD142 pKa = 3.46 GGGTGPAPSCPPEE155 pKa = 4.43 GVLPQHH161 pKa = 6.72 HH162 pKa = 6.63 SAFKK166 pKa = 10.47 PIDD169 pKa = 4.07 PDD171 pKa = 3.78 SFTPCATGKK180 pKa = 8.35 PTFSGSPAASSSIGSFPWISSGTQDD205 pKa = 3.24 TPKK208 pKa = 10.44 ISMTMSSSYY217 pKa = 11.01 LGFYY221 pKa = 9.57 ATDD224 pKa = 3.54 TYY226 pKa = 10.71 ITTGQNKK233 pKa = 9.61 RR234 pKa = 11.84 SVSVIGLGRR243 pKa = 11.84 LTVTEE248 pKa = 4.85 DD249 pKa = 3.27 GRR251 pKa = 11.84 SSAVVTEE258 pKa = 4.64 AEE260 pKa = 4.78 PIHH263 pKa = 6.38 HH264 pKa = 6.9 SSLIGYY270 pKa = 8.77 QSTGTGITGTVSFFRR285 pKa = 11.84 TTGSEE290 pKa = 4.08 DD291 pKa = 3.28 TADD294 pKa = 3.82 AQDD297 pKa = 3.37 VGIRR301 pKa = 11.84 SHH303 pKa = 6.24 TLLVMRR309 pKa = 11.84 RR310 pKa = 11.84 NRR312 pKa = 11.84 KK313 pKa = 9.08 LDD315 pKa = 3.31 TYY317 pKa = 10.16 VYY319 pKa = 10.32 EE320 pKa = 4.54 GGEE323 pKa = 3.97 IQGGDD328 pKa = 4.39 MYY330 pKa = 11.37 MMASPLSGQGTMVGDD345 pKa = 3.72 EE346 pKa = 4.31 MVLLGYY352 pKa = 10.07 AVPVTNYY359 pKa = 10.0 AGPVWCPNTFCNTQTMEE376 pKa = 3.91 ACKK379 pKa = 10.14 NASGTIRR386 pKa = 11.84 FPGSSTGLAVVMVRR400 pKa = 11.84 YY401 pKa = 8.7 MIQDD405 pKa = 3.66 LDD407 pKa = 3.81 KK408 pKa = 11.61 DD409 pKa = 3.88 GFKK412 pKa = 10.83 PKK414 pKa = 9.14 ITIHH418 pKa = 5.28 MLNRR422 pKa = 11.84 EE423 pKa = 4.02 QWPIPGPYY431 pKa = 9.71 HH432 pKa = 5.82 LTSGTKK438 pKa = 9.73 GLFISVYY445 pKa = 7.86 SWGWYY450 pKa = 6.75 GHH452 pKa = 6.1 PVSGEE457 pKa = 3.8 INRR460 pKa = 11.84 ATWDD464 pKa = 3.3 MRR466 pKa = 11.84 SIRR469 pKa = 11.84 LVYY472 pKa = 10.54 ALLWPDD478 pKa = 3.48 LPEE481 pKa = 4.45 TEE483 pKa = 4.5 GKK485 pKa = 10.77 CYY487 pKa = 10.5 TNLANPCPRR496 pKa = 11.84 ACTIGSTRR504 pKa = 11.84 IGSGIPLTFTGQLSAWLTISADD526 pKa = 3.42 GGATRR531 pKa = 11.84 LKK533 pKa = 10.63 PEE535 pKa = 4.0 VAVSYY540 pKa = 10.81 SDD542 pKa = 3.74 PSYY545 pKa = 11.02 HH546 pKa = 7.24 DD547 pKa = 3.68 LVEE550 pKa = 3.83 RR551 pKa = 11.84 TYY553 pKa = 10.69 PYY555 pKa = 7.96 TTAATFTAVEE565 pKa = 4.17 GSITCFMVSGLPQCITLIEE584 pKa = 4.68 GDD586 pKa = 3.54 SSAYY590 pKa = 8.52 ATNLQTYY597 pKa = 7.43 NLWSLPIDD605 pKa = 4.1 CPSTRR610 pKa = 11.84 NLTLTTNTTSPP621 pKa = 3.51
Molecular weight: 66.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.424
IPC2_protein 5.461
IPC_protein 5.486
Toseland 5.804
ProMoST 5.804
Dawson 5.703
Bjellqvist 5.741
Wikipedia 5.69
Rodwell 5.664
Grimsley 5.906
Solomon 5.703
Lehninger 5.69
Nozaki 5.944
DTASelect 6.135
Thurlkill 6.084
EMBOSS 6.059
Sillero 6.033
Patrickios 3.63
IPC_peptide 5.715
IPC2_peptide 6.046
IPC2.peptide.svr19 5.984
Protein with the highest isoelectric point:
>tr|A0A2P1GMY6|A0A2P1GMY6_9MONO Nucleocapsid protein OS=Wenling triplecross lizardfish paramyxovirus OX=2116451 PE=3 SV=1
MM1 pKa = 7.33 SGKK4 pKa = 9.72 SAPLWDD10 pKa = 4.45 GCWSGGGCRR19 pKa = 11.84 DD20 pKa = 3.65 NIEE23 pKa = 4.04 IPKK26 pKa = 9.85 PDD28 pKa = 3.75 IEE30 pKa = 4.95 KK31 pKa = 10.76 GPIPITYY38 pKa = 9.07 QVPVPSDD45 pKa = 3.53 PGNKK49 pKa = 10.2 LITQLTLFGFIEE61 pKa = 4.28 YY62 pKa = 10.31 KK63 pKa = 10.88 YY64 pKa = 9.63 MSKK67 pKa = 10.83 NSGGEE72 pKa = 3.8 KK73 pKa = 9.97 KK74 pKa = 10.53 GVSRR78 pKa = 11.84 IILPCSMPRR87 pKa = 11.84 GDD89 pKa = 4.54 PDD91 pKa = 3.3 IAQMSHH97 pKa = 7.22 DD98 pKa = 4.39 LSQIEE103 pKa = 3.93 ISVIRR108 pKa = 11.84 RR109 pKa = 11.84 PRR111 pKa = 11.84 AIEE114 pKa = 4.29 SIGFCADD121 pKa = 3.4 SVPDD125 pKa = 3.88 SLSEE129 pKa = 4.05 HH130 pKa = 6.81 LGFLLNGMGFPAYY143 pKa = 9.35 KK144 pKa = 10.4 VSTGVDD150 pKa = 3.22 KK151 pKa = 11.01 MPASSKK157 pKa = 10.46 YY158 pKa = 10.76 KK159 pKa = 9.43 FIIWAGVLTALSGPGGYY176 pKa = 9.15 VVPIRR181 pKa = 11.84 RR182 pKa = 11.84 MIGGVAKK189 pKa = 10.25 NRR191 pKa = 11.84 PSGPEE196 pKa = 3.37 VKK198 pKa = 9.63 MSLKK202 pKa = 9.08 ITLKK206 pKa = 9.94 TSFDD210 pKa = 3.45 IKK212 pKa = 10.94 ALAINAEE219 pKa = 4.17 KK220 pKa = 10.63 CDD222 pKa = 3.95 DD223 pKa = 4.65 GGWSTDD229 pKa = 2.89 VLLYY233 pKa = 10.42 IGTISRR239 pKa = 11.84 RR240 pKa = 11.84 NGRR243 pKa = 11.84 PVDD246 pKa = 3.38 TEE248 pKa = 3.97 YY249 pKa = 11.56 AKK251 pKa = 11.07 NFASGLGFKK260 pKa = 10.54 LGLGSVGGVSIHH272 pKa = 5.32 VHH274 pKa = 4.57 ATGLKK279 pKa = 8.82 WKK281 pKa = 10.52 ASLNKK286 pKa = 8.12 TTGGKK291 pKa = 10.23 KK292 pKa = 10.19 NICFPLAEE300 pKa = 4.26 VAPVLNRR307 pKa = 11.84 VLACRR312 pKa = 11.84 DD313 pKa = 3.28 TAIIDD318 pKa = 4.21 AEE320 pKa = 4.26 ICFQATDD327 pKa = 3.43 MRR329 pKa = 11.84 DD330 pKa = 3.36 VRR332 pKa = 11.84 EE333 pKa = 4.29 TTSVVHH339 pKa = 6.19 TLPKK343 pKa = 10.57 DD344 pKa = 3.73 VIVVNLSHH352 pKa = 7.14
Molecular weight: 37.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.289
IPC2_protein 8.244
IPC_protein 8.112
Toseland 8.492
ProMoST 8.668
Dawson 9.019
Bjellqvist 9.077
Wikipedia 9.048
Rodwell 9.165
Grimsley 8.492
Solomon 9.121
Lehninger 9.107
Nozaki 9.326
DTASelect 8.887
Thurlkill 9.019
EMBOSS 9.194
Sillero 9.253
Patrickios 4.787
IPC_peptide 9.121
IPC2_peptide 7.834
IPC2.peptide.svr19 7.786
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
5064
118
2180
723.4
80.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.385 ± 0.889
1.54 ± 0.292
6.319 ± 0.465
5.569 ± 0.672
2.942 ± 0.208
6.754 ± 0.776
2.31 ± 0.34
7.366 ± 0.556
4.976 ± 0.514
9.36 ± 0.639
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.16 ± 0.229
3.752 ± 0.26
4.562 ± 0.502
3.456 ± 0.437
5.766 ± 0.474
7.721 ± 0.431
6.991 ± 0.747
6.062 ± 0.419
1.106 ± 0.211
2.903 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here