Achromobacter sp. AONIH1
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5889 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I8DF85|A0A2I8DF85_9BURK IclR family transcriptional regulator OS=Achromobacter sp. AONIH1 OX=1758194 GN=C2U31_05015 PE=4 SV=1
MM1 pKa = 7.12 RR2 pKa = 11.84 TWMCLICGWVYY13 pKa = 11.09 DD14 pKa = 4.61 EE15 pKa = 4.94 EE16 pKa = 6.63 AGLPDD21 pKa = 3.89 EE22 pKa = 5.81 GIAPGTRR29 pKa = 11.84 WEE31 pKa = 4.42 DD32 pKa = 3.53 VPPNWVCPEE41 pKa = 3.8 CGARR45 pKa = 11.84 KK46 pKa = 9.54 EE47 pKa = 4.36 DD48 pKa = 3.84 FEE50 pKa = 5.52 LMEE53 pKa = 4.22 II54 pKa = 4.3
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.837
IPC2_protein 4.088
IPC_protein 3.923
Toseland 3.77
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.783
Rodwell 3.77
Grimsley 3.681
Solomon 3.859
Lehninger 3.808
Nozaki 4.012
DTASelect 4.113
Thurlkill 3.808
EMBOSS 3.795
Sillero 4.037
Patrickios 1.888
IPC_peptide 3.859
IPC2_peptide 4.024
IPC2.peptide.svr19 3.93
Protein with the highest isoelectric point:
>tr|A0A2I8DDR5|A0A2I8DDR5_9BURK ABC transporter ATP-binding protein OS=Achromobacter sp. AONIH1 OX=1758194 GN=C2U31_03740 PE=4 SV=1
MM1 pKa = 7.07 QGVHH5 pKa = 6.72 IGPARR10 pKa = 11.84 IARR13 pKa = 11.84 PAGLVRR19 pKa = 11.84 VVRR22 pKa = 11.84 LPGISRR28 pKa = 11.84 LPRR31 pKa = 11.84 LARR34 pKa = 11.84 LAGLPRR40 pKa = 11.84 LVRR43 pKa = 11.84 LAGHH47 pKa = 6.4 FRR49 pKa = 11.84 RR50 pKa = 11.84 HH51 pKa = 4.94 AARR54 pKa = 11.84 LIVVLPGFAWLLLLTGGLLGRR75 pKa = 11.84 RR76 pKa = 11.84 VAGRR80 pKa = 11.84 MSRR83 pKa = 11.84 VHH85 pKa = 6.7 GGLLKK90 pKa = 10.24 IRR92 pKa = 11.84 ARR94 pKa = 11.84 RR95 pKa = 11.84 YY96 pKa = 8.89 RR97 pKa = 3.43
Molecular weight: 10.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.345
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.486
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.193
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.206
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5889
0
5889
1952274
29
10627
331.5
35.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.491 ± 0.046
0.889 ± 0.012
5.411 ± 0.027
4.95 ± 0.037
3.281 ± 0.023
8.72 ± 0.06
2.064 ± 0.021
4.307 ± 0.021
2.775 ± 0.028
11.0 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.403 ± 0.017
2.549 ± 0.028
5.368 ± 0.029
3.892 ± 0.021
7.384 ± 0.041
5.383 ± 0.03
4.857 ± 0.036
7.365 ± 0.03
1.459 ± 0.014
2.454 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here