Gordonia phage Eyes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FFS7|A0A3G8FFS7_9CAUD Uncharacterized protein OS=Gordonia phage Eyes OX=2488782 GN=40 PE=4 SV=1
MM1 pKa = 6.78TTPPPTFTLPEE12 pKa = 4.26YY13 pKa = 10.33PGVTYY18 pKa = 9.85TVLTGSGGVPGLPSNAVRR36 pKa = 11.84IVGTVVNPWHH46 pKa = 6.14EE47 pKa = 3.88PEE49 pKa = 4.27YY50 pKa = 11.2NYY52 pKa = 11.36GLDD55 pKa = 3.51PNGYY59 pKa = 7.8TEE61 pKa = 5.28VTDD64 pKa = 3.13PWKK67 pKa = 10.71RR68 pKa = 11.84HH69 pKa = 4.33TEE71 pKa = 4.27LIEE74 pKa = 3.91VTNTGFLAPTAPVPTEE90 pKa = 4.34PPPSPPAPTPDD101 pKa = 3.85PEE103 pKa = 4.27PTPEE107 pKa = 3.9EE108 pKa = 4.23VV109 pKa = 3.16

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FFP8|A0A3G8FFP8_9CAUD FtsK-like DNA translocase OS=Gordonia phage Eyes OX=2488782 GN=52 PE=4 SV=1
MM1 pKa = 7.32ARR3 pKa = 11.84LRR5 pKa = 11.84ARR7 pKa = 11.84LTALLIAALAAATLSACDD25 pKa = 3.18SDD27 pKa = 5.4EE28 pKa = 4.02YY29 pKa = 11.5VEE31 pKa = 4.29PVSNEE36 pKa = 3.4ICVDD40 pKa = 3.25RR41 pKa = 11.84SDD43 pKa = 5.77RR44 pKa = 11.84RR45 pKa = 11.84AQDD48 pKa = 3.57HH49 pKa = 5.83YY50 pKa = 11.34CEE52 pKa = 4.72SGLPNYY58 pKa = 9.08HH59 pKa = 5.15WWYY62 pKa = 10.29LPAGTYY68 pKa = 10.33VGIGQHH74 pKa = 4.81VTGGTNLRR82 pKa = 11.84PNRR85 pKa = 11.84VRR87 pKa = 11.84RR88 pKa = 11.84APTVTVTKK96 pKa = 9.32KK97 pKa = 5.53TTPRR101 pKa = 11.84TTATTAARR109 pKa = 11.84KK110 pKa = 5.43PTTRR114 pKa = 11.84ATPTKK119 pKa = 9.13TRR121 pKa = 11.84ARR123 pKa = 11.84STRR126 pKa = 3.24

Molecular weight: 13.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

14927

48

1706

229.6

24.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.662 ± 0.559

0.657 ± 0.138

6.592 ± 0.283

5.694 ± 0.374

2.733 ± 0.222

8.468 ± 0.46

1.889 ± 0.161

4.267 ± 0.213

2.854 ± 0.193

8.106 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.148

2.787 ± 0.162

6.016 ± 0.399

3.403 ± 0.189

7.764 ± 0.406

5.721 ± 0.183

6.612 ± 0.315

7.423 ± 0.297

2.144 ± 0.126

2.01 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski