Amino acid dipepetide frequency for Gordonia phage Eyes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.491AlaAla: 18.491 ± 1.508
0.67AlaCys: 0.67 ± 0.187
8.643AlaAsp: 8.643 ± 0.798
6.968AlaGlu: 6.968 ± 0.988
2.948AlaPhe: 2.948 ± 0.503
10.854AlaGly: 10.854 ± 1.198
2.278AlaHis: 2.278 ± 0.467
5.159AlaIle: 5.159 ± 0.449
4.087AlaLys: 4.087 ± 0.598
10.184AlaLeu: 10.184 ± 0.885
3.082AlaMet: 3.082 ± 0.528
3.417AlaAsn: 3.417 ± 0.344
6.901AlaPro: 6.901 ± 0.631
4.556AlaGln: 4.556 ± 0.687
10.05AlaArg: 10.05 ± 0.934
6.566AlaSer: 6.566 ± 0.672
9.313AlaThr: 9.313 ± 0.937
8.777AlaVal: 8.777 ± 1.112
1.809AlaTrp: 1.809 ± 0.322
1.876AlaTyr: 1.876 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
0.268CysAla: 0.268 ± 0.141
0.067CysCys: 0.067 ± 0.07
0.804CysAsp: 0.804 ± 0.238
0.402CysGlu: 0.402 ± 0.19
0.0CysPhe: 0.0 ± 0.0
0.67CysGly: 0.67 ± 0.193
0.134CysHis: 0.134 ± 0.11
0.134CysIle: 0.134 ± 0.101
0.067CysLys: 0.067 ± 0.063
0.469CysLeu: 0.469 ± 0.257
0.0CysMet: 0.0 ± 0.0
0.402CysAsn: 0.402 ± 0.163
0.603CysPro: 0.603 ± 0.282
0.335CysGln: 0.335 ± 0.149
0.67CysArg: 0.67 ± 0.253
0.201CysSer: 0.201 ± 0.113
0.469CysThr: 0.469 ± 0.205
0.335CysVal: 0.335 ± 0.125
0.402CysTrp: 0.402 ± 0.165
0.134CysTyr: 0.134 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
8.308AspAla: 8.308 ± 0.607
0.67AspCys: 0.67 ± 0.284
5.293AspAsp: 5.293 ± 0.764
6.231AspGlu: 6.231 ± 0.597
2.412AspPhe: 2.412 ± 0.363
5.561AspGly: 5.561 ± 0.634
1.675AspHis: 1.675 ± 0.304
1.943AspIle: 1.943 ± 0.368
1.139AspLys: 1.139 ± 0.345
6.097AspLeu: 6.097 ± 0.778
0.804AspMet: 0.804 ± 0.272
1.34AspAsn: 1.34 ± 0.295
4.355AspPro: 4.355 ± 0.71
2.479AspGln: 2.479 ± 0.387
4.891AspArg: 4.891 ± 0.795
3.216AspSer: 3.216 ± 0.426
2.814AspThr: 2.814 ± 0.484
3.953AspVal: 3.953 ± 0.532
1.407AspTrp: 1.407 ± 0.255
1.34AspTyr: 1.34 ± 0.28
0.0AspXaa: 0.0 ± 0.0
Glu
6.499GluAla: 6.499 ± 0.847
0.201GluCys: 0.201 ± 0.123
3.35GluAsp: 3.35 ± 0.483
2.814GluGlu: 2.814 ± 0.498
2.077GluPhe: 2.077 ± 0.53
4.02GluGly: 4.02 ± 0.564
1.072GluHis: 1.072 ± 0.297
2.814GluIle: 2.814 ± 0.58
1.206GluLys: 1.206 ± 0.252
5.092GluLeu: 5.092 ± 0.654
1.139GluMet: 1.139 ± 0.252
1.407GluAsn: 1.407 ± 0.363
3.819GluPro: 3.819 ± 0.784
2.278GluGln: 2.278 ± 0.388
5.092GluArg: 5.092 ± 0.771
3.015GluSer: 3.015 ± 0.406
3.417GluThr: 3.417 ± 0.457
3.819GluVal: 3.819 ± 0.547
1.742GluTrp: 1.742 ± 0.407
2.077GluTyr: 2.077 ± 0.404
0.0GluXaa: 0.0 ± 0.0
Phe
3.283PheAla: 3.283 ± 0.557
0.0PheCys: 0.0 ± 0.0
2.144PheAsp: 2.144 ± 0.333
2.479PheGlu: 2.479 ± 0.421
0.67PhePhe: 0.67 ± 0.161
2.68PheGly: 2.68 ± 0.403
0.402PheHis: 0.402 ± 0.14
1.139PheIle: 1.139 ± 0.293
0.469PheLys: 0.469 ± 0.19
2.747PheLeu: 2.747 ± 0.588
0.134PheMet: 0.134 ± 0.08
0.469PheAsn: 0.469 ± 0.239
1.273PhePro: 1.273 ± 0.304
0.536PheGln: 0.536 ± 0.156
1.608PheArg: 1.608 ± 0.389
1.608PheSer: 1.608 ± 0.428
2.077PheThr: 2.077 ± 0.565
2.479PheVal: 2.479 ± 0.404
0.603PheTrp: 0.603 ± 0.154
0.536PheTyr: 0.536 ± 0.198
0.0PheXaa: 0.0 ± 0.0
Gly
8.241GlyAla: 8.241 ± 0.903
0.536GlyCys: 0.536 ± 0.17
5.494GlyAsp: 5.494 ± 0.642
4.087GlyGlu: 4.087 ± 0.538
2.747GlyPhe: 2.747 ± 0.493
8.174GlyGly: 8.174 ± 0.941
2.01GlyHis: 2.01 ± 0.317
4.087GlyIle: 4.087 ± 0.908
2.613GlyLys: 2.613 ± 0.45
8.107GlyLeu: 8.107 ± 0.694
2.211GlyMet: 2.211 ± 0.353
1.943GlyAsn: 1.943 ± 0.341
3.35GlyPro: 3.35 ± 0.497
3.149GlyGln: 3.149 ± 0.442
6.164GlyArg: 6.164 ± 0.823
5.494GlySer: 5.494 ± 0.692
5.427GlyThr: 5.427 ± 0.657
6.767GlyVal: 6.767 ± 0.599
1.474GlyTrp: 1.474 ± 0.296
2.613GlyTyr: 2.613 ± 0.418
0.0GlyXaa: 0.0 ± 0.0
His
2.144HisAla: 2.144 ± 0.418
0.268HisCys: 0.268 ± 0.152
1.005HisAsp: 1.005 ± 0.263
1.273HisGlu: 1.273 ± 0.351
0.536HisPhe: 0.536 ± 0.214
1.273HisGly: 1.273 ± 0.459
0.67HisHis: 0.67 ± 0.234
0.737HisIle: 0.737 ± 0.244
0.536HisLys: 0.536 ± 0.205
1.943HisLeu: 1.943 ± 0.414
0.268HisMet: 0.268 ± 0.128
0.469HisAsn: 0.469 ± 0.19
1.608HisPro: 1.608 ± 0.259
0.469HisGln: 0.469 ± 0.184
1.072HisArg: 1.072 ± 0.272
1.407HisSer: 1.407 ± 0.297
0.871HisThr: 0.871 ± 0.25
1.34HisVal: 1.34 ± 0.259
0.469HisTrp: 0.469 ± 0.165
0.536HisTyr: 0.536 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
6.767IleAla: 6.767 ± 0.683
0.268IleCys: 0.268 ± 0.122
2.814IleAsp: 2.814 ± 0.473
2.948IleGlu: 2.948 ± 0.537
0.938IlePhe: 0.938 ± 0.207
3.618IleGly: 3.618 ± 0.541
1.005IleHis: 1.005 ± 0.275
1.608IleIle: 1.608 ± 0.298
1.407IleLys: 1.407 ± 0.476
2.211IleLeu: 2.211 ± 0.398
0.335IleMet: 0.335 ± 0.205
1.206IleAsn: 1.206 ± 0.226
2.211IlePro: 2.211 ± 0.372
1.809IleGln: 1.809 ± 0.352
2.68IleArg: 2.68 ± 0.417
2.412IleSer: 2.412 ± 0.494
2.948IleThr: 2.948 ± 0.376
3.216IleVal: 3.216 ± 0.466
1.139IleTrp: 1.139 ± 0.276
1.072IleTyr: 1.072 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
2.881LysAla: 2.881 ± 0.537
0.268LysCys: 0.268 ± 0.128
1.876LysAsp: 1.876 ± 0.504
0.67LysGlu: 0.67 ± 0.204
1.072LysPhe: 1.072 ± 0.307
2.278LysGly: 2.278 ± 0.404
0.335LysHis: 0.335 ± 0.167
1.139LysIle: 1.139 ± 0.282
1.742LysLys: 1.742 ± 0.449
2.881LysLeu: 2.881 ± 0.478
0.536LysMet: 0.536 ± 0.199
0.67LysAsn: 0.67 ± 0.165
1.273LysPro: 1.273 ± 0.316
0.737LysGln: 0.737 ± 0.286
2.144LysArg: 2.144 ± 0.417
1.876LysSer: 1.876 ± 0.421
2.211LysThr: 2.211 ± 0.41
2.68LysVal: 2.68 ± 0.476
0.871LysTrp: 0.871 ± 0.233
0.402LysTyr: 0.402 ± 0.151
0.0LysXaa: 0.0 ± 0.0
Leu
11.323LeuAla: 11.323 ± 0.847
0.536LeuCys: 0.536 ± 0.222
4.489LeuAsp: 4.489 ± 0.601
4.556LeuGlu: 4.556 ± 0.933
2.211LeuPhe: 2.211 ± 0.403
6.365LeuGly: 6.365 ± 0.669
1.742LeuHis: 1.742 ± 0.429
2.546LeuIle: 2.546 ± 0.422
1.809LeuLys: 1.809 ± 0.419
6.03LeuLeu: 6.03 ± 0.874
1.742LeuMet: 1.742 ± 0.369
2.01LeuAsn: 2.01 ± 0.341
5.226LeuPro: 5.226 ± 0.673
2.345LeuGln: 2.345 ± 0.423
7.102LeuArg: 7.102 ± 0.796
5.36LeuSer: 5.36 ± 0.633
5.829LeuThr: 5.829 ± 0.697
6.566LeuVal: 6.566 ± 0.772
1.541LeuTrp: 1.541 ± 0.271
1.742LeuTyr: 1.742 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
3.551MetAla: 3.551 ± 0.588
0.201MetCys: 0.201 ± 0.125
1.34MetAsp: 1.34 ± 0.295
0.469MetGlu: 0.469 ± 0.174
0.335MetPhe: 0.335 ± 0.164
1.541MetGly: 1.541 ± 0.355
0.536MetHis: 0.536 ± 0.238
1.34MetIle: 1.34 ± 0.292
0.402MetLys: 0.402 ± 0.143
1.809MetLeu: 1.809 ± 0.356
0.536MetMet: 0.536 ± 0.216
1.072MetAsn: 1.072 ± 0.336
1.139MetPro: 1.139 ± 0.277
0.469MetGln: 0.469 ± 0.177
1.742MetArg: 1.742 ± 0.352
1.608MetSer: 1.608 ± 0.318
2.01MetThr: 2.01 ± 0.467
1.139MetVal: 1.139 ± 0.251
0.67MetTrp: 0.67 ± 0.253
0.067MetTyr: 0.067 ± 0.075
0.0MetXaa: 0.0 ± 0.0
Asn
3.752AsnAla: 3.752 ± 0.69
0.067AsnCys: 0.067 ± 0.071
1.809AsnAsp: 1.809 ± 0.318
1.072AsnGlu: 1.072 ± 0.235
0.603AsnPhe: 0.603 ± 0.245
3.551AsnGly: 3.551 ± 0.558
0.469AsnHis: 0.469 ± 0.21
0.67AsnIle: 0.67 ± 0.268
0.603AsnLys: 0.603 ± 0.242
2.01AsnLeu: 2.01 ± 0.34
0.536AsnMet: 0.536 ± 0.173
0.938AsnAsn: 0.938 ± 0.212
2.278AsnPro: 2.278 ± 0.424
1.139AsnGln: 1.139 ± 0.298
2.613AsnArg: 2.613 ± 0.503
1.34AsnSer: 1.34 ± 0.309
1.407AsnThr: 1.407 ± 0.374
1.608AsnVal: 1.608 ± 0.325
0.737AsnTrp: 0.737 ± 0.172
0.67AsnTyr: 0.67 ± 0.206
0.0AsnXaa: 0.0 ± 0.0
Pro
8.04ProAla: 8.04 ± 0.91
0.335ProCys: 0.335 ± 0.14
4.556ProAsp: 4.556 ± 0.65
3.417ProGlu: 3.417 ± 0.518
1.608ProPhe: 1.608 ± 0.404
5.762ProGly: 5.762 ± 0.714
0.67ProHis: 0.67 ± 0.24
2.144ProIle: 2.144 ± 0.354
1.742ProLys: 1.742 ± 0.336
3.618ProLeu: 3.618 ± 0.454
1.206ProMet: 1.206 ± 0.325
1.742ProAsn: 1.742 ± 0.377
4.422ProPro: 4.422 ± 0.974
2.211ProGln: 2.211 ± 0.507
3.618ProArg: 3.618 ± 0.615
3.819ProSer: 3.819 ± 0.611
4.489ProThr: 4.489 ± 0.702
4.489ProVal: 4.489 ± 0.609
1.675ProTrp: 1.675 ± 0.397
0.603ProTyr: 0.603 ± 0.203
0.0ProXaa: 0.0 ± 0.0
Gln
4.422GlnAla: 4.422 ± 0.811
0.469GlnCys: 0.469 ± 0.181
1.608GlnAsp: 1.608 ± 0.326
1.273GlnGlu: 1.273 ± 0.337
1.005GlnPhe: 1.005 ± 0.215
2.546GlnGly: 2.546 ± 0.477
0.871GlnHis: 0.871 ± 0.308
2.412GlnIle: 2.412 ± 0.53
1.34GlnLys: 1.34 ± 0.311
3.149GlnLeu: 3.149 ± 0.393
1.273GlnMet: 1.273 ± 0.29
0.938GlnAsn: 0.938 ± 0.256
1.005GlnPro: 1.005 ± 0.291
1.742GlnGln: 1.742 ± 0.4
2.814GlnArg: 2.814 ± 0.44
1.675GlnSer: 1.675 ± 0.391
1.742GlnThr: 1.742 ± 0.345
2.278GlnVal: 2.278 ± 0.42
0.938GlnTrp: 0.938 ± 0.266
0.536GlnTyr: 0.536 ± 0.193
0.0GlnXaa: 0.0 ± 0.0
Arg
9.045ArgAla: 9.045 ± 0.987
0.67ArgCys: 0.67 ± 0.276
5.226ArgAsp: 5.226 ± 0.423
3.953ArgGlu: 3.953 ± 0.656
1.876ArgPhe: 1.876 ± 0.353
5.561ArgGly: 5.561 ± 0.659
1.139ArgHis: 1.139 ± 0.254
3.551ArgIle: 3.551 ± 0.502
3.149ArgLys: 3.149 ± 0.495
6.499ArgLeu: 6.499 ± 0.845
3.216ArgMet: 3.216 ± 0.558
2.948ArgAsn: 2.948 ± 0.496
4.422ArgPro: 4.422 ± 0.833
3.283ArgGln: 3.283 ± 0.516
8.107ArgArg: 8.107 ± 1.149
4.221ArgSer: 4.221 ± 0.492
4.623ArgThr: 4.623 ± 0.521
4.02ArgVal: 4.02 ± 0.452
0.938ArgTrp: 0.938 ± 0.257
1.206ArgTyr: 1.206 ± 0.369
0.0ArgXaa: 0.0 ± 0.0
Ser
7.102SerAla: 7.102 ± 0.644
0.134SerCys: 0.134 ± 0.101
3.417SerAsp: 3.417 ± 0.525
3.752SerGlu: 3.752 ± 0.594
1.005SerPhe: 1.005 ± 0.313
6.03SerGly: 6.03 ± 0.626
1.072SerHis: 1.072 ± 0.265
2.747SerIle: 2.747 ± 0.382
1.541SerLys: 1.541 ± 0.339
4.154SerLeu: 4.154 ± 0.516
1.876SerMet: 1.876 ± 0.42
1.608SerAsn: 1.608 ± 0.283
3.819SerPro: 3.819 ± 0.577
1.273SerGln: 1.273 ± 0.344
3.819SerArg: 3.819 ± 0.601
3.149SerSer: 3.149 ± 0.518
3.953SerThr: 3.953 ± 0.51
4.824SerVal: 4.824 ± 0.701
1.005SerTrp: 1.005 ± 0.247
0.938SerTyr: 0.938 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
7.638ThrAla: 7.638 ± 1.055
0.402ThrCys: 0.402 ± 0.18
4.69ThrAsp: 4.69 ± 0.634
3.886ThrGlu: 3.886 ± 0.53
1.943ThrPhe: 1.943 ± 0.396
5.963ThrGly: 5.963 ± 0.693
0.67ThrHis: 0.67 ± 0.27
3.283ThrIle: 3.283 ± 0.511
0.737ThrLys: 0.737 ± 0.226
4.824ThrLeu: 4.824 ± 0.704
0.737ThrMet: 0.737 ± 0.236
1.608ThrAsn: 1.608 ± 0.284
5.36ThrPro: 5.36 ± 0.598
2.278ThrGln: 2.278 ± 0.426
3.685ThrArg: 3.685 ± 0.459
4.221ThrSer: 4.221 ± 0.474
4.891ThrThr: 4.891 ± 0.639
6.499ThrVal: 6.499 ± 0.817
1.206ThrTrp: 1.206 ± 0.286
1.608ThrTyr: 1.608 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
10.653ValAla: 10.653 ± 1.211
0.335ValCys: 0.335 ± 0.142
4.489ValAsp: 4.489 ± 0.586
4.288ValGlu: 4.288 ± 0.626
1.943ValPhe: 1.943 ± 0.321
5.628ValGly: 5.628 ± 0.593
0.871ValHis: 0.871 ± 0.254
3.551ValIle: 3.551 ± 0.493
2.546ValLys: 2.546 ± 0.448
5.427ValLeu: 5.427 ± 0.614
1.34ValMet: 1.34 ± 0.311
2.01ValAsn: 2.01 ± 0.337
4.891ValPro: 4.891 ± 0.54
1.742ValGln: 1.742 ± 0.284
6.432ValArg: 6.432 ± 0.705
4.087ValSer: 4.087 ± 0.642
5.025ValThr: 5.025 ± 0.643
6.097ValVal: 6.097 ± 0.76
1.742ValTrp: 1.742 ± 0.35
1.139ValTyr: 1.139 ± 0.29
0.0ValXaa: 0.0 ± 0.0
Trp
2.278TrpAla: 2.278 ± 0.378
0.268TrpCys: 0.268 ± 0.12
1.675TrpAsp: 1.675 ± 0.266
1.072TrpGlu: 1.072 ± 0.296
0.871TrpPhe: 0.871 ± 0.192
1.34TrpGly: 1.34 ± 0.329
0.67TrpHis: 0.67 ± 0.259
0.938TrpIle: 0.938 ± 0.219
0.737TrpLys: 0.737 ± 0.241
2.077TrpLeu: 2.077 ± 0.405
0.804TrpMet: 0.804 ± 0.213
1.206TrpAsn: 1.206 ± 0.318
1.072TrpPro: 1.072 ± 0.3
0.737TrpGln: 0.737 ± 0.206
1.474TrpArg: 1.474 ± 0.274
1.005TrpSer: 1.005 ± 0.264
1.072TrpThr: 1.072 ± 0.255
1.072TrpVal: 1.072 ± 0.249
0.67TrpTrp: 0.67 ± 0.228
0.402TrpTyr: 0.402 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.943TyrAla: 1.943 ± 0.371
0.201TyrCys: 0.201 ± 0.117
1.206TyrAsp: 1.206 ± 0.318
1.34TyrGlu: 1.34 ± 0.412
0.536TyrPhe: 0.536 ± 0.201
1.273TyrGly: 1.273 ± 0.282
0.603TyrHis: 0.603 ± 0.226
0.737TyrIle: 0.737 ± 0.257
0.67TyrLys: 0.67 ± 0.2
1.742TyrLeu: 1.742 ± 0.367
0.134TyrMet: 0.134 ± 0.085
0.469TyrAsn: 0.469 ± 0.177
1.139TyrPro: 1.139 ± 0.341
0.469TyrGln: 0.469 ± 0.164
1.876TyrArg: 1.876 ± 0.413
0.938TyrSer: 0.938 ± 0.223
1.541TyrThr: 1.541 ± 0.421
2.278TyrVal: 2.278 ± 0.452
0.402TyrTrp: 0.402 ± 0.155
0.603TyrTyr: 0.603 ± 0.179
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (14927 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski