Stieleria varia

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Stieleria

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6986 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6B145|A0A5C6B145_9BACT Mycocerosic acid synthase OS=Stieleria varia OX=2528005 GN=mas_1 PE=4 SV=1
MM1 pKa = 7.38TSILHH6 pKa = 5.36VFAGTFLSRR15 pKa = 11.84NEE17 pKa = 3.69ACLYY21 pKa = 9.58TEE23 pKa = 4.6PQWEE27 pKa = 4.55PEE29 pKa = 4.05PSDD32 pKa = 3.45TVSDD36 pKa = 3.84DD37 pKa = 4.94EE38 pKa = 4.35YY39 pKa = 11.56AAWEE43 pKa = 4.08NRR45 pKa = 11.84NPSWGFCDD53 pKa = 5.04DD54 pKa = 4.56LGDD57 pKa = 3.96VYY59 pKa = 11.18LDD61 pKa = 3.27SDD63 pKa = 4.54FIEE66 pKa = 5.09TIDD69 pKa = 3.41GDD71 pKa = 3.65EE72 pKa = 4.36RR73 pKa = 11.84YY74 pKa = 10.19SYY76 pKa = 10.42LASYY80 pKa = 9.48LVNGSDD86 pKa = 3.8LGRR89 pKa = 11.84VRR91 pKa = 11.84AANPDD96 pKa = 3.79ANTLLLVFPDD106 pKa = 3.48AFGGFDD112 pKa = 3.17SSLRR116 pKa = 11.84STPKK120 pKa = 9.41LTYY123 pKa = 10.38CGGFDD128 pKa = 4.75FRR130 pKa = 11.84WPP132 pKa = 3.41

Molecular weight: 14.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6AV68|A0A5C6AV68_9BACT Outer membrane biogenesis protein BamB OS=Stieleria varia OX=2528005 GN=Pla52n_40200 PE=4 SV=1
MM1 pKa = 7.74LEE3 pKa = 4.38LPFGSTYY10 pKa = 10.74EE11 pKa = 4.06EE12 pKa = 4.34PKK14 pKa = 10.57VAMSGDD20 pKa = 3.15RR21 pKa = 11.84LVNVTGVSRR30 pKa = 11.84RR31 pKa = 11.84QRR33 pKa = 11.84CQSTIGEE40 pKa = 4.33SRR42 pKa = 11.84KK43 pKa = 8.61RR44 pKa = 11.84TLLNDD49 pKa = 3.26STTTSFKK56 pKa = 10.79HH57 pKa = 4.22NTRR60 pKa = 11.84RR61 pKa = 11.84LPVWNTWIKK70 pKa = 9.76TRR72 pKa = 11.84ACTRR76 pKa = 11.84NGFEE80 pKa = 4.55SRR82 pKa = 11.84PASLVVYY89 pKa = 10.33LMLLNDD95 pKa = 4.6DD96 pKa = 4.91LVANHH101 pKa = 6.19TRR103 pKa = 11.84LTARR107 pKa = 11.84LDD109 pKa = 3.72YY110 pKa = 9.12ITRR113 pKa = 11.84RR114 pKa = 11.84SRR116 pKa = 11.84VRR118 pKa = 11.84IPSKK122 pKa = 9.81RR123 pKa = 11.84TFGSLCSSVGRR134 pKa = 11.84ASNCLIDD141 pKa = 4.18NFVDD145 pKa = 3.79RR146 pKa = 11.84FYY148 pKa = 11.63KK149 pKa = 10.7SMSEE153 pKa = 4.31GPTWPAQTRR162 pKa = 11.84HH163 pKa = 6.16DD164 pKa = 3.7

Molecular weight: 18.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6986

0

6986

2762882

29

13990

395.5

43.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.409 ± 0.032

1.137 ± 0.017

6.628 ± 0.039

5.79 ± 0.03

3.653 ± 0.02

7.622 ± 0.051

2.193 ± 0.02

5.194 ± 0.023

3.344 ± 0.034

9.528 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.021

3.324 ± 0.029

5.139 ± 0.023

4.221 ± 0.021

6.567 ± 0.043

7.122 ± 0.029

5.866 ± 0.049

7.226 ± 0.032

1.467 ± 0.015

2.294 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski