Streptococcus phage phi-SsuFJNP9_rum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3MMB6|A0A7G3MMB6_9CAUD DNA primase OS=Streptococcus phage phi-SsuFJNP9_rum OX=2664392 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.52LKK4 pKa = 10.66DD5 pKa = 3.35ILEE8 pKa = 4.55LGTYY12 pKa = 9.43GFNPDD17 pKa = 3.62CKK19 pKa = 10.98VEE21 pKa = 4.13IFNMDD26 pKa = 3.22NFEE29 pKa = 4.08EE30 pKa = 4.3RR31 pKa = 11.84LEE33 pKa = 4.14NEE35 pKa = 4.24GFDD38 pKa = 5.31EE39 pKa = 5.2ILIPQNEE46 pKa = 4.18DD47 pKa = 2.73AKK49 pKa = 10.24IYY51 pKa = 9.99PYY53 pKa = 11.33AFLIEE58 pKa = 4.24DD59 pKa = 4.28SILIAMTEE67 pKa = 3.91EE68 pKa = 4.47DD69 pKa = 5.42DD70 pKa = 4.4NTNEE74 pKa = 4.2CC75 pKa = 4.51

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3MIM3|A0A7G3MIM3_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuFJNP9_rum OX=2664392 PE=4 SV=1
MM1 pKa = 7.74PRR3 pKa = 11.84RR4 pKa = 11.84PSTPCKK10 pKa = 10.55HH11 pKa = 6.34NGCPNLVPYY20 pKa = 8.51GQKK23 pKa = 10.43YY24 pKa = 9.67CDD26 pKa = 3.23NHH28 pKa = 6.29RR29 pKa = 11.84ANHH32 pKa = 5.5QLDD35 pKa = 3.8AKK37 pKa = 8.36STKK40 pKa = 10.16AKK42 pKa = 10.17GYY44 pKa = 9.01NVQWNKK50 pKa = 10.23ARR52 pKa = 11.84LRR54 pKa = 11.84YY55 pKa = 9.99LKK57 pKa = 9.81VHH59 pKa = 6.46PLCVQCKK66 pKa = 8.68VKK68 pKa = 10.55GRR70 pKa = 11.84LTKK73 pKa = 10.1ATVVDD78 pKa = 5.28HH79 pKa = 6.23ITPHH83 pKa = 6.97RR84 pKa = 11.84GDD86 pKa = 4.62QEE88 pKa = 4.85LFWDD92 pKa = 3.75QSNWQALCKK101 pKa = 10.09SCHH104 pKa = 5.64DD105 pKa = 4.22RR106 pKa = 11.84KK107 pKa = 8.93TKK109 pKa = 8.08TTDD112 pKa = 2.41RR113 pKa = 11.84HH114 pKa = 5.41VEE116 pKa = 3.59YY117 pKa = 9.4TYY119 pKa = 11.2RR120 pKa = 11.84FF121 pKa = 3.56

Molecular weight: 14.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

17222

42

1493

253.3

28.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.503 ± 0.406

0.877 ± 0.112

5.748 ± 0.232

7.636 ± 0.325

4.204 ± 0.218

6.225 ± 0.242

1.771 ± 0.125

7.136 ± 0.264

7.485 ± 0.243

9.018 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.147

5.063 ± 0.176

3.072 ± 0.181

4.21 ± 0.23

4.477 ± 0.156

6.521 ± 0.296

6.184 ± 0.472

6.259 ± 0.24

1.365 ± 0.104

3.902 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski