Flavisolibacter tropicus
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4588 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A172U0A7|A0A172U0A7_9BACT CBAH domain-containing protein OS=Flavisolibacter tropicus OX=1492898 GN=SY85_22210 PE=3 SV=1
MM1 pKa = 7.77 KK2 pKa = 10.32 FGNWKK7 pKa = 8.11 VTQDD11 pKa = 3.63 GIVWKK16 pKa = 10.63 GPGYY20 pKa = 10.8 NEE22 pKa = 3.7 FVIPATEE29 pKa = 4.15 LVAEE33 pKa = 4.34 RR34 pKa = 11.84 PGLLSTPTTYY44 pKa = 10.46 EE45 pKa = 3.52 WIMRR49 pKa = 11.84 ATDD52 pKa = 3.65 EE53 pKa = 4.16 HH54 pKa = 6.9 WLTEE58 pKa = 4.29 DD59 pKa = 4.96 DD60 pKa = 6.24 LYY62 pKa = 11.6 DD63 pKa = 3.73 LNYY66 pKa = 11.0 AFVFAAARR74 pKa = 11.84 YY75 pKa = 8.2 GLNFNYY81 pKa = 8.04 EE82 pKa = 4.26 TFDD85 pKa = 3.45 NTLEE89 pKa = 4.07 EE90 pKa = 4.09 QYY92 pKa = 11.38 EE93 pKa = 4.2 RR94 pKa = 11.84 FDD96 pKa = 5.85 DD97 pKa = 5.4 EE98 pKa = 6.48 DD99 pKa = 6.26 DD100 pKa = 4.83 EE101 pKa = 7.82 DD102 pKa = 6.44 DD103 pKa = 5.39 DD104 pKa = 6.89 DD105 pKa = 4.35 YY106 pKa = 12.28
Molecular weight: 12.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.77
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.859
Patrickios 1.863
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A172TZG0|A0A172TZG0_9BACT Thioredoxin OS=Flavisolibacter tropicus OX=1492898 GN=SY85_20330 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPHH8 pKa = 4.58 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 6.91 TVHH16 pKa = 6.14 GFRR19 pKa = 11.84 KK20 pKa = 10.11 RR21 pKa = 11.84 MQTANGRR28 pKa = 11.84 KK29 pKa = 8.71 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 HH40 pKa = 5.59 KK41 pKa = 10.51 LTVSDD46 pKa = 4.17 EE47 pKa = 4.26 KK48 pKa = 10.99 RR49 pKa = 11.84 LKK51 pKa = 10.55
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 10.745
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.252
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.974
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4588
0
4588
1527984
38
3798
333.0
37.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.545 ± 0.043
0.847 ± 0.015
5.029 ± 0.026
5.687 ± 0.047
4.831 ± 0.027
6.781 ± 0.035
1.935 ± 0.02
6.802 ± 0.03
6.723 ± 0.041
9.517 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.242 ± 0.016
5.437 ± 0.035
3.88 ± 0.022
4.23 ± 0.024
4.084 ± 0.026
6.344 ± 0.033
6.127 ± 0.057
6.543 ± 0.03
1.3 ± 0.015
4.117 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here