Tortoise microvirus 77

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W6V8|A0A4P8W6V8_9VIRU Uncharacterized protein OS=Tortoise microvirus 77 OX=2583184 PE=4 SV=1
MM1 pKa = 7.64AIKK4 pKa = 10.69VGDD7 pKa = 3.66LNRR10 pKa = 11.84WKK12 pKa = 10.49KK13 pKa = 10.54LKK15 pKa = 10.46PGGALEE21 pKa = 4.53LRR23 pKa = 11.84SDD25 pKa = 3.43KK26 pKa = 10.47QRR28 pKa = 11.84QVRR31 pKa = 11.84LEE33 pKa = 4.19VNVDD37 pKa = 3.14QPTQVMALRR46 pKa = 11.84GTDD49 pKa = 3.53LTLLGVVMGMDD60 pKa = 3.85VIEE63 pKa = 4.67FVTDD67 pKa = 4.02GPTEE71 pKa = 4.24VVFSTEE77 pKa = 3.84GEE79 pKa = 4.21VFYY82 pKa = 8.06FTRR85 pKa = 11.84DD86 pKa = 3.26GDD88 pKa = 4.46SEE90 pKa = 4.76ALDD93 pKa = 3.82LLEE96 pKa = 4.49EE97 pKa = 4.29VSFATLANRR106 pKa = 11.84RR107 pKa = 11.84ARR109 pKa = 11.84NPEE112 pKa = 3.68MEE114 pKa = 4.67LMFFKK119 pKa = 10.4MNQNIEE125 pKa = 3.92RR126 pKa = 11.84RR127 pKa = 11.84LAIQADD133 pKa = 3.84DD134 pKa = 3.78FEE136 pKa = 6.05RR137 pKa = 11.84RR138 pKa = 11.84LQEE141 pKa = 3.7MGYY144 pKa = 10.63DD145 pKa = 3.73PDD147 pKa = 3.78TGEE150 pKa = 5.07VSDD153 pKa = 5.67ADD155 pKa = 3.83DD156 pKa = 3.74TGTAGGDD163 pKa = 3.24TGEE166 pKa = 4.97AEE168 pKa = 4.23AEE170 pKa = 4.14SGGVGGADD178 pKa = 3.15AEE180 pKa = 4.56APGDD184 pKa = 3.71AASAPGGGAGTAA196 pKa = 3.64

Molecular weight:
20.99 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4P8W6K2|A0A4P8W6K2_9VIRU Peptidase_M15_4 domain-containing protein OS=Tortoise microvirus 77 OX=2583184 PE=4 SV=1
MM1 pKa = 7.19QSVAKK6 pKa = 10.25DD7 pKa = 3.27SRR9 pKa = 11.84EE10 pKa = 3.76NPGRR14 pKa = 11.84NRR16 pKa = 11.84ISTSKK21 pKa = 10.4IGGQTTGAPRR31 pKa = 11.84KK32 pKa = 5.62TTAAAPAKK40 pKa = 10.11QSAPARR46 pKa = 11.84KK47 pKa = 8.02ATPKK51 pKa = 10.25AAPDD55 pKa = 4.71PIRR58 pKa = 11.84RR59 pKa = 11.84ATQAAPSRR67 pKa = 11.84ATLEE71 pKa = 4.17RR72 pKa = 11.84QAGLPAQPARR82 pKa = 11.84RR83 pKa = 11.84SVPSHH88 pKa = 6.57SSSEE92 pKa = 4.2SLSGSEE98 pKa = 4.74GGEE101 pKa = 4.01TTLGRR106 pKa = 11.84TARR109 pKa = 11.84EE110 pKa = 3.62RR111 pKa = 11.84LAQQAVQPPRR121 pKa = 11.84APDD124 pKa = 3.71PVILAPTNPITPVNPVQEE142 pKa = 4.17AARR145 pKa = 11.84ARR147 pKa = 11.84RR148 pKa = 11.84VHH150 pKa = 6.14SLPGGTLRR158 pKa = 11.84GTQNNTTRR166 pKa = 11.84TVTAKK171 pKa = 10.6AKK173 pKa = 10.56PGDD176 pKa = 3.62AKK178 pKa = 11.04ARR180 pKa = 11.84TQVQPHH186 pKa = 6.15KK187 pKa = 10.43AARR190 pKa = 11.84DD191 pKa = 3.42DD192 pKa = 3.9RR193 pKa = 11.84EE194 pKa = 3.65PDD196 pKa = 3.05KK197 pKa = 11.41RR198 pKa = 11.84LVCKK202 pKa = 10.46ARR204 pKa = 11.84PEE206 pKa = 4.32SNKK209 pKa = 10.04PKK211 pKa = 10.61GGGGGAKK218 pKa = 9.72RR219 pKa = 11.84AFIPWCSS226 pKa = 3.07

Molecular weight:
23.75 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1769

74

537

252.7

27.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.91 ± 1.115

0.17 ± 0.139

5.596 ± 0.658

6.162 ± 0.995

3.222 ± 0.516

9.723 ± 1.052

1.922 ± 0.604

3.166 ± 0.289

4.409 ± 0.584

7.349 ± 0.811

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.445

3.505 ± 0.765

6.897 ± 1.059

4.635 ± 0.399

7.405 ± 0.995

5.427 ± 0.622

5.709 ± 0.805

7.01 ± 1.248

2.374 ± 0.664

2.261 ± 0.643

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski