Tortoise microvirus 77
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6V8|A0A4P8W6V8_9VIRU Uncharacterized protein OS=Tortoise microvirus 77 OX=2583184 PE=4 SV=1
MM1 pKa = 7.64 AIKK4 pKa = 10.69 VGDD7 pKa = 3.66 LNRR10 pKa = 11.84 WKK12 pKa = 10.49 KK13 pKa = 10.54 LKK15 pKa = 10.46 PGGALEE21 pKa = 4.53 LRR23 pKa = 11.84 SDD25 pKa = 3.43 KK26 pKa = 10.47 QRR28 pKa = 11.84 QVRR31 pKa = 11.84 LEE33 pKa = 4.19 VNVDD37 pKa = 3.14 QPTQVMALRR46 pKa = 11.84 GTDD49 pKa = 3.53 LTLLGVVMGMDD60 pKa = 3.85 VIEE63 pKa = 4.67 FVTDD67 pKa = 4.02 GPTEE71 pKa = 4.24 VVFSTEE77 pKa = 3.84 GEE79 pKa = 4.21 VFYY82 pKa = 8.06 FTRR85 pKa = 11.84 DD86 pKa = 3.26 GDD88 pKa = 4.46 SEE90 pKa = 4.76 ALDD93 pKa = 3.82 LLEE96 pKa = 4.49 EE97 pKa = 4.29 VSFATLANRR106 pKa = 11.84 RR107 pKa = 11.84 ARR109 pKa = 11.84 NPEE112 pKa = 3.68 MEE114 pKa = 4.67 LMFFKK119 pKa = 10.4 MNQNIEE125 pKa = 3.92 RR126 pKa = 11.84 RR127 pKa = 11.84 LAIQADD133 pKa = 3.84 DD134 pKa = 3.78 FEE136 pKa = 6.05 RR137 pKa = 11.84 RR138 pKa = 11.84 LQEE141 pKa = 3.7 MGYY144 pKa = 10.63 DD145 pKa = 3.73 PDD147 pKa = 3.78 TGEE150 pKa = 5.07 VSDD153 pKa = 5.67 ADD155 pKa = 3.83 DD156 pKa = 3.74 TGTAGGDD163 pKa = 3.24 TGEE166 pKa = 4.97 AEE168 pKa = 4.23 AEE170 pKa = 4.14 SGGVGGADD178 pKa = 3.15 AEE180 pKa = 4.56 APGDD184 pKa = 3.71 AASAPGGGAGTAA196 pKa = 3.64
Molecular weight: 20.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.487
IPC2_protein 4.19
IPC_protein 4.139
Toseland 3.948
ProMoST 4.266
Dawson 4.101
Bjellqvist 4.253
Wikipedia 3.999
Rodwell 3.973
Grimsley 3.859
Solomon 4.101
Lehninger 4.05
Nozaki 4.215
DTASelect 4.406
Thurlkill 3.973
EMBOSS 4.012
Sillero 4.253
Patrickios 4.139
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.189
Protein with the highest isoelectric point:
>tr|A0A4P8W6K2|A0A4P8W6K2_9VIRU Peptidase_M15_4 domain-containing protein OS=Tortoise microvirus 77 OX=2583184 PE=4 SV=1
MM1 pKa = 7.19 QSVAKK6 pKa = 10.25 DD7 pKa = 3.27 SRR9 pKa = 11.84 EE10 pKa = 3.76 NPGRR14 pKa = 11.84 NRR16 pKa = 11.84 ISTSKK21 pKa = 10.4 IGGQTTGAPRR31 pKa = 11.84 KK32 pKa = 5.62 TTAAAPAKK40 pKa = 10.11 QSAPARR46 pKa = 11.84 KK47 pKa = 8.02 ATPKK51 pKa = 10.25 AAPDD55 pKa = 4.71 PIRR58 pKa = 11.84 RR59 pKa = 11.84 ATQAAPSRR67 pKa = 11.84 ATLEE71 pKa = 4.17 RR72 pKa = 11.84 QAGLPAQPARR82 pKa = 11.84 RR83 pKa = 11.84 SVPSHH88 pKa = 6.57 SSSEE92 pKa = 4.2 SLSGSEE98 pKa = 4.74 GGEE101 pKa = 4.01 TTLGRR106 pKa = 11.84 TARR109 pKa = 11.84 EE110 pKa = 3.62 RR111 pKa = 11.84 LAQQAVQPPRR121 pKa = 11.84 APDD124 pKa = 3.71 PVILAPTNPITPVNPVQEE142 pKa = 4.17 AARR145 pKa = 11.84 ARR147 pKa = 11.84 RR148 pKa = 11.84 VHH150 pKa = 6.14 SLPGGTLRR158 pKa = 11.84 GTQNNTTRR166 pKa = 11.84 TVTAKK171 pKa = 10.6 AKK173 pKa = 10.56 PGDD176 pKa = 3.62 AKK178 pKa = 11.04 ARR180 pKa = 11.84 TQVQPHH186 pKa = 6.15 KK187 pKa = 10.43 AARR190 pKa = 11.84 DD191 pKa = 3.42 DD192 pKa = 3.9 RR193 pKa = 11.84 EE194 pKa = 3.65 PDD196 pKa = 3.05 KK197 pKa = 11.41 RR198 pKa = 11.84 LVCKK202 pKa = 10.46 ARR204 pKa = 11.84 PEE206 pKa = 4.32 SNKK209 pKa = 10.04 PKK211 pKa = 10.61 GGGGGAKK218 pKa = 9.72 RR219 pKa = 11.84 AFIPWCSS226 pKa = 3.07
Molecular weight: 23.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.97
IPC_protein 11.418
Toseland 11.637
ProMoST 12.076
Dawson 11.652
Bjellqvist 11.594
Wikipedia 12.076
Rodwell 11.564
Grimsley 11.681
Solomon 12.091
Lehninger 12.003
Nozaki 11.637
DTASelect 11.594
Thurlkill 11.637
EMBOSS 12.12
Sillero 11.637
Patrickios 11.286
IPC_peptide 12.106
IPC2_peptide 11.067
IPC2.peptide.svr19 9.437
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1769
74
537
252.7
27.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.91 ± 1.115
0.17 ± 0.139
5.596 ± 0.658
6.162 ± 0.995
3.222 ± 0.516
9.723 ± 1.052
1.922 ± 0.604
3.166 ± 0.289
4.409 ± 0.584
7.349 ± 0.811
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.148 ± 0.445
3.505 ± 0.765
6.897 ± 1.059
4.635 ± 0.399
7.405 ± 0.995
5.427 ± 0.622
5.709 ± 0.805
7.01 ± 1.248
2.374 ± 0.664
2.261 ± 0.643
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here