Clostridium hiranonis (strain DSM 13275 / JCM 10541 / KCTC 15199 / TO-931)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Peptacetobacter; Peptacetobacter hiranonis

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6FXN3|B6FXN3_CLOHT Macro domain protein OS=Clostridium hiranonis (strain DSM 13275 / JCM 10541 / KCTC 15199 / TO-931) OX=500633 GN=CLOHIR_00632 PE=4 SV=1
MM1 pKa = 7.79PSKK4 pKa = 10.6EE5 pKa = 3.97WLTKK9 pKa = 10.35YY10 pKa = 9.76EE11 pKa = 4.31SKK13 pKa = 10.75KK14 pKa = 10.35EE15 pKa = 3.94SLRR18 pKa = 11.84CKK20 pKa = 9.95IDD22 pKa = 3.16LDD24 pKa = 4.66AYY26 pKa = 9.44FTEE29 pKa = 4.25NKK31 pKa = 9.27IGKK34 pKa = 9.52INLDD38 pKa = 3.33VLNIGKK44 pKa = 9.88VSFPTGKK51 pKa = 9.97IIACDD56 pKa = 3.57PLIEE60 pKa = 5.82LEE62 pKa = 4.65DD63 pKa = 3.92APAYY67 pKa = 8.96IQNIPPGNYY76 pKa = 9.55NVDD79 pKa = 2.79ISVALSEE86 pKa = 4.52KK87 pKa = 10.61FGDD90 pKa = 3.51RR91 pKa = 11.84YY92 pKa = 11.13SCVKK96 pKa = 10.66VLINDD101 pKa = 3.89NKK103 pKa = 9.26PAYY106 pKa = 9.68YY107 pKa = 10.42DD108 pKa = 3.38LGVIGNEE115 pKa = 4.1DD116 pKa = 3.81LDD118 pKa = 4.33EE119 pKa = 4.31EE120 pKa = 5.1LEE122 pKa = 4.22DD123 pKa = 4.37DD124 pKa = 4.59EE125 pKa = 5.94YY126 pKa = 11.67FGFCVDD132 pKa = 4.51AGMGCILDD140 pKa = 4.36EE141 pKa = 4.24STQAAFKK148 pKa = 10.15EE149 pKa = 4.49YY150 pKa = 9.85WEE152 pKa = 4.06QRR154 pKa = 11.84CEE156 pKa = 4.07KK157 pKa = 10.62EE158 pKa = 4.54EE159 pKa = 5.26GIDD162 pKa = 3.57PYY164 pKa = 11.92NDD166 pKa = 3.95LFCDD170 pKa = 4.01LLEE173 pKa = 4.37EE174 pKa = 5.36SYY176 pKa = 11.58DD177 pKa = 3.97KK178 pKa = 11.1YY179 pKa = 10.92PKK181 pKa = 9.22YY182 pKa = 10.25QRR184 pKa = 11.84EE185 pKa = 4.2GGDD188 pKa = 3.25WLNWAIPEE196 pKa = 4.28TEE198 pKa = 3.96LNIPIFASGWGDD210 pKa = 3.2GVYY213 pKa = 9.9PVYY216 pKa = 10.51FGYY219 pKa = 10.8DD220 pKa = 3.68DD221 pKa = 4.07NDD223 pKa = 4.23EE224 pKa = 4.24ICGVYY229 pKa = 10.14ILFIDD234 pKa = 3.96VTDD237 pKa = 3.94DD238 pKa = 3.39TFDD241 pKa = 3.15

Molecular weight: 27.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6G1V8|B6G1V8_CLOHT Homoserine O-acetyltransferase OS=Clostridium hiranonis (strain DSM 13275 / JCM 10541 / KCTC 15199 / TO-931) OX=500633 GN=metA PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.08GFRR19 pKa = 11.84KK20 pKa = 9.93RR21 pKa = 11.84MRR23 pKa = 11.84SANGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.19GRR39 pKa = 11.84KK40 pKa = 8.95RR41 pKa = 11.84LTHH44 pKa = 6.22

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2271

0

2271

693674

39

2932

305.4

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.269 ± 0.058

1.243 ± 0.023

6.073 ± 0.041

8.278 ± 0.068

4.234 ± 0.039

6.71 ± 0.053

1.309 ± 0.018

9.081 ± 0.067

9.067 ± 0.057

8.29 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.997 ± 0.032

5.679 ± 0.046

2.734 ± 0.025

2.07 ± 0.021

3.633 ± 0.033

5.973 ± 0.044

4.865 ± 0.038

6.995 ± 0.05

0.58 ± 0.013

3.922 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski