Amino acid dipepetide frequency for Clostridium hiranonis (strain DSM 13275 / JCM 10541 / KCTC 15199 / TO-931)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.818AlaAla: 4.818 ± 0.118
0.846AlaCys: 0.846 ± 0.043
3.484AlaAsp: 3.484 ± 0.078
4.678AlaGlu: 4.678 ± 0.091
2.51AlaPhe: 2.51 ± 0.064
4.406AlaGly: 4.406 ± 0.105
0.819AlaHis: 0.819 ± 0.031
6.001AlaIle: 6.001 ± 0.109
5.389AlaLys: 5.389 ± 0.103
5.556AlaLeu: 5.556 ± 0.099
2.083AlaMet: 2.083 ± 0.061
2.913AlaAsn: 2.913 ± 0.078
1.612AlaPro: 1.612 ± 0.064
1.39AlaGln: 1.39 ± 0.049
2.232AlaArg: 2.232 ± 0.061
3.35AlaSer: 3.35 ± 0.076
3.319AlaThr: 3.319 ± 0.073
4.917AlaVal: 4.917 ± 0.094
0.321AlaTrp: 0.321 ± 0.021
2.041AlaTyr: 2.041 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.741CysAla: 0.741 ± 0.032
0.235CysCys: 0.235 ± 0.022
0.768CysAsp: 0.768 ± 0.033
0.887CysGlu: 0.887 ± 0.039
0.476CysPhe: 0.476 ± 0.03
1.306CysGly: 1.306 ± 0.051
0.215CysHis: 0.215 ± 0.018
1.034CysIle: 1.034 ± 0.042
0.986CysLys: 0.986 ± 0.04
0.84CysLeu: 0.84 ± 0.038
0.402CysMet: 0.402 ± 0.024
0.58CysAsn: 0.58 ± 0.027
0.546CysPro: 0.546 ± 0.032
0.242CysGln: 0.242 ± 0.019
0.479CysArg: 0.479 ± 0.025
0.815CysSer: 0.815 ± 0.034
0.6CysThr: 0.6 ± 0.03
0.829CysVal: 0.829 ± 0.043
0.072CysTrp: 0.072 ± 0.009
0.375CysTyr: 0.375 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.492AspAla: 3.492 ± 0.087
0.771AspCys: 0.771 ± 0.036
3.533AspAsp: 3.533 ± 0.095
5.68AspGlu: 5.68 ± 0.105
2.991AspPhe: 2.991 ± 0.075
4.032AspGly: 4.032 ± 0.081
0.607AspHis: 0.607 ± 0.035
6.195AspIle: 6.195 ± 0.099
5.726AspLys: 5.726 ± 0.094
4.858AspLeu: 4.858 ± 0.098
1.961AspMet: 1.961 ± 0.053
2.999AspAsn: 2.999 ± 0.079
1.401AspPro: 1.401 ± 0.048
0.822AspGln: 0.822 ± 0.033
2.321AspArg: 2.321 ± 0.062
3.293AspSer: 3.293 ± 0.075
2.693AspThr: 2.693 ± 0.07
4.188AspVal: 4.188 ± 0.091
0.394AspTrp: 0.394 ± 0.027
2.771AspTyr: 2.771 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
4.675GluAla: 4.675 ± 0.094
0.879GluCys: 0.879 ± 0.039
5.059GluAsp: 5.059 ± 0.103
8.257GluGlu: 8.257 ± 0.158
3.432GluPhe: 3.432 ± 0.087
4.609GluGly: 4.609 ± 0.093
1.006GluHis: 1.006 ± 0.037
7.7GluIle: 7.7 ± 0.125
8.971GluLys: 8.971 ± 0.131
6.953GluLeu: 6.953 ± 0.103
2.529GluMet: 2.529 ± 0.059
5.831GluAsn: 5.831 ± 0.108
1.506GluPro: 1.506 ± 0.051
1.753GluGln: 1.753 ± 0.048
3.108GluArg: 3.108 ± 0.079
3.914GluSer: 3.914 ± 0.073
3.29GluThr: 3.29 ± 0.071
5.351GluVal: 5.351 ± 0.099
0.437GluTrp: 0.437 ± 0.025
3.518GluTyr: 3.518 ± 0.091
0.0GluXaa: 0.0 ± 0.0
Phe
2.686PheAla: 2.686 ± 0.064
0.588PheCys: 0.588 ± 0.029
2.844PheAsp: 2.844 ± 0.07
3.417PheGlu: 3.417 ± 0.08
1.78PhePhe: 1.78 ± 0.058
3.073PheGly: 3.073 ± 0.081
0.441PheHis: 0.441 ± 0.027
3.859PheIle: 3.859 ± 0.089
3.404PheLys: 3.404 ± 0.085
3.558PheLeu: 3.558 ± 0.077
1.284PheMet: 1.284 ± 0.049
2.392PheAsn: 2.392 ± 0.064
1.1PhePro: 1.1 ± 0.033
0.657PheGln: 0.657 ± 0.031
1.419PheArg: 1.419 ± 0.056
2.743PheSer: 2.743 ± 0.066
2.224PheThr: 2.224 ± 0.06
3.04PheVal: 3.04 ± 0.079
0.259PheTrp: 0.259 ± 0.021
1.566PheTyr: 1.566 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.413GlyAla: 4.413 ± 0.103
1.096GlyCys: 1.096 ± 0.045
3.529GlyAsp: 3.529 ± 0.084
4.727GlyGlu: 4.727 ± 0.093
3.193GlyPhe: 3.193 ± 0.076
4.744GlyGly: 4.744 ± 0.132
0.99GlyHis: 0.99 ± 0.043
6.646GlyIle: 6.646 ± 0.108
6.065GlyLys: 6.065 ± 0.102
5.482GlyLeu: 5.482 ± 0.1
2.131GlyMet: 2.131 ± 0.056
3.456GlyAsn: 3.456 ± 0.067
1.186GlyPro: 1.186 ± 0.045
1.388GlyGln: 1.388 ± 0.049
2.39GlyArg: 2.39 ± 0.07
3.683GlySer: 3.683 ± 0.081
3.513GlyThr: 3.513 ± 0.08
5.01GlyVal: 5.01 ± 0.101
0.463GlyTrp: 0.463 ± 0.03
2.993GlyTyr: 2.993 ± 0.068
0.0GlyXaa: 0.0 ± 0.0
His
0.757HisAla: 0.757 ± 0.037
0.19HisCys: 0.19 ± 0.017
0.699HisAsp: 0.699 ± 0.031
0.992HisGlu: 0.992 ± 0.037
0.626HisPhe: 0.626 ± 0.031
0.936HisGly: 0.936 ± 0.039
0.281HisHis: 0.281 ± 0.028
1.234HisIle: 1.234 ± 0.045
0.972HisLys: 0.972 ± 0.036
1.041HisLeu: 1.041 ± 0.039
0.334HisMet: 0.334 ± 0.02
0.693HisAsn: 0.693 ± 0.032
0.592HisPro: 0.592 ± 0.034
0.327HisGln: 0.327 ± 0.021
0.5HisArg: 0.5 ± 0.026
0.829HisSer: 0.829 ± 0.04
0.706HisThr: 0.706 ± 0.034
0.793HisVal: 0.793 ± 0.041
0.101HisTrp: 0.101 ± 0.013
0.486HisTyr: 0.486 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.095IleAla: 6.095 ± 0.108
1.176IleCys: 1.176 ± 0.041
6.017IleAsp: 6.017 ± 0.099
7.437IleGlu: 7.437 ± 0.13
3.737IlePhe: 3.737 ± 0.094
5.882IleGly: 5.882 ± 0.105
1.165IleHis: 1.165 ± 0.04
7.612IleIle: 7.612 ± 0.13
8.242IleLys: 8.242 ± 0.126
7.518IleLeu: 7.518 ± 0.126
2.461IleMet: 2.461 ± 0.063
4.937IleAsn: 4.937 ± 0.105
3.238IlePro: 3.238 ± 0.074
1.786IleGln: 1.786 ± 0.054
2.968IleArg: 2.968 ± 0.069
6.255IleSer: 6.255 ± 0.111
4.489IleThr: 4.489 ± 0.089
6.249IleVal: 6.249 ± 0.094
0.418IleTrp: 0.418 ± 0.028
3.125IleTyr: 3.125 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
5.229LysAla: 5.229 ± 0.106
0.859LysCys: 0.859 ± 0.038
5.771LysAsp: 5.771 ± 0.11
9.304LysGlu: 9.304 ± 0.147
3.327LysPhe: 3.327 ± 0.076
4.991LysGly: 4.991 ± 0.089
1.055LysHis: 1.055 ± 0.043
7.919LysIle: 7.919 ± 0.134
8.913LysLys: 8.913 ± 0.126
6.917LysLeu: 6.917 ± 0.095
2.918LysMet: 2.918 ± 0.069
6.246LysAsn: 6.246 ± 0.116
2.09LysPro: 2.09 ± 0.057
1.847LysGln: 1.847 ± 0.057
3.395LysArg: 3.395 ± 0.08
4.975LysSer: 4.975 ± 0.085
4.156LysThr: 4.156 ± 0.08
6.029LysVal: 6.029 ± 0.095
0.539LysTrp: 0.539 ± 0.028
4.191LysTyr: 4.191 ± 0.075
0.0LysXaa: 0.0 ± 0.0
Leu
5.461LeuAla: 5.461 ± 0.097
0.954LeuCys: 0.954 ± 0.042
5.507LeuAsp: 5.507 ± 0.105
6.731LeuGlu: 6.731 ± 0.118
3.412LeuPhe: 3.412 ± 0.093
5.736LeuGly: 5.736 ± 0.113
1.009LeuHis: 1.009 ± 0.038
6.564LeuIle: 6.564 ± 0.113
7.818LeuLys: 7.818 ± 0.119
6.741LeuLeu: 6.741 ± 0.121
2.335LeuMet: 2.335 ± 0.065
4.824LeuAsn: 4.824 ± 0.071
2.458LeuPro: 2.458 ± 0.065
1.694LeuGln: 1.694 ± 0.047
2.849LeuArg: 2.849 ± 0.08
5.73LeuSer: 5.73 ± 0.09
4.165LeuThr: 4.165 ± 0.092
5.681LeuVal: 5.681 ± 0.092
0.438LeuTrp: 0.438 ± 0.025
2.794LeuTyr: 2.794 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
1.998MetAla: 1.998 ± 0.059
0.327MetCys: 0.327 ± 0.025
1.838MetAsp: 1.838 ± 0.051
2.273MetGlu: 2.273 ± 0.063
1.156MetPhe: 1.156 ± 0.044
2.074MetGly: 2.074 ± 0.058
0.365MetHis: 0.365 ± 0.024
2.582MetIle: 2.582 ± 0.062
2.919MetLys: 2.919 ± 0.066
2.539MetLeu: 2.539 ± 0.059
0.891MetMet: 0.891 ± 0.042
1.883MetAsn: 1.883 ± 0.054
0.938MetPro: 0.938 ± 0.036
0.734MetGln: 0.734 ± 0.034
1.096MetArg: 1.096 ± 0.037
1.979MetSer: 1.979 ± 0.057
1.42MetThr: 1.42 ± 0.046
1.815MetVal: 1.815 ± 0.049
0.154MetTrp: 0.154 ± 0.015
0.986MetTyr: 0.986 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
3.192AsnAla: 3.192 ± 0.078
0.714AsnCys: 0.714 ± 0.041
2.981AsnAsp: 2.981 ± 0.071
4.048AsnGlu: 4.048 ± 0.08
2.194AsnPhe: 2.194 ± 0.056
4.0AsnGly: 4.0 ± 0.092
0.724AsnHis: 0.724 ± 0.03
5.774AsnIle: 5.774 ± 0.123
5.23AsnLys: 5.23 ± 0.098
4.922AsnLeu: 4.922 ± 0.107
1.867AsnMet: 1.867 ± 0.05
3.257AsnAsn: 3.257 ± 0.119
2.171AsnPro: 2.171 ± 0.054
1.208AsnGln: 1.208 ± 0.046
2.282AsnArg: 2.282 ± 0.062
3.314AsnSer: 3.314 ± 0.086
2.821AsnThr: 2.821 ± 0.065
3.584AsnVal: 3.584 ± 0.073
0.368AsnTrp: 0.368 ± 0.022
2.142AsnTyr: 2.142 ± 0.066
0.0AsnXaa: 0.0 ± 0.0
Pro
1.589ProAla: 1.589 ± 0.055
0.349ProCys: 0.349 ± 0.024
1.648ProAsp: 1.648 ± 0.058
2.605ProGlu: 2.605 ± 0.061
1.302ProPhe: 1.302 ± 0.04
1.78ProGly: 1.78 ± 0.052
0.443ProHis: 0.443 ± 0.028
2.521ProIle: 2.521 ± 0.065
2.135ProLys: 2.135 ± 0.054
2.116ProLeu: 2.116 ± 0.067
0.75ProMet: 0.75 ± 0.032
1.44ProAsn: 1.44 ± 0.05
0.509ProPro: 0.509 ± 0.03
0.691ProGln: 0.691 ± 0.033
0.842ProArg: 0.842 ± 0.035
1.646ProSer: 1.646 ± 0.054
1.491ProThr: 1.491 ± 0.05
2.236ProVal: 2.236 ± 0.058
0.183ProTrp: 0.183 ± 0.017
1.068ProTyr: 1.068 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
1.426GlnAla: 1.426 ± 0.054
0.185GlnCys: 0.185 ± 0.016
1.034GlnAsp: 1.034 ± 0.037
1.531GlnGlu: 1.531 ± 0.045
0.8GlnPhe: 0.8 ± 0.035
1.344GlnGly: 1.344 ± 0.047
0.309GlnHis: 0.309 ± 0.019
1.945GlnIle: 1.945 ± 0.057
1.831GlnLys: 1.831 ± 0.054
1.858GlnLeu: 1.858 ± 0.059
0.683GlnMet: 0.683 ± 0.033
1.29GlnAsn: 1.29 ± 0.043
0.545GlnPro: 0.545 ± 0.029
0.538GlnGln: 0.538 ± 0.031
0.892GlnArg: 0.892 ± 0.034
1.155GlnSer: 1.155 ± 0.037
1.028GlnThr: 1.028 ± 0.036
1.309GlnVal: 1.309 ± 0.043
0.143GlnTrp: 0.143 ± 0.012
0.852GlnTyr: 0.852 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.115ArgAla: 2.115 ± 0.051
0.443ArgCys: 0.443 ± 0.027
2.109ArgAsp: 2.109 ± 0.059
3.417ArgGlu: 3.417 ± 0.073
1.599ArgPhe: 1.599 ± 0.048
2.209ArgGly: 2.209 ± 0.051
0.48ArgHis: 0.48 ± 0.026
3.265ArgIle: 3.265 ± 0.077
3.486ArgLys: 3.486 ± 0.076
2.86ArgLeu: 2.86 ± 0.06
1.1ArgMet: 1.1 ± 0.046
2.152ArgAsn: 2.152 ± 0.057
0.976ArgPro: 0.976 ± 0.04
0.875ArgGln: 0.875 ± 0.039
1.643ArgArg: 1.643 ± 0.049
1.488ArgSer: 1.488 ± 0.044
1.632ArgThr: 1.632 ± 0.048
2.593ArgVal: 2.593 ± 0.063
0.2ArgTrp: 0.2 ± 0.018
1.684ArgTyr: 1.684 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
3.554SerAla: 3.554 ± 0.08
0.688SerCys: 0.688 ± 0.037
3.598SerAsp: 3.598 ± 0.086
4.277SerGlu: 4.277 ± 0.086
2.684SerPhe: 2.684 ± 0.064
4.462SerGly: 4.462 ± 0.088
0.796SerHis: 0.796 ± 0.032
5.769SerIle: 5.769 ± 0.111
5.354SerLys: 5.354 ± 0.091
5.096SerLeu: 5.096 ± 0.084
1.728SerMet: 1.728 ± 0.057
3.182SerAsn: 3.182 ± 0.083
1.46SerPro: 1.46 ± 0.047
1.364SerGln: 1.364 ± 0.045
2.17SerArg: 2.17 ± 0.057
3.94SerSer: 3.94 ± 0.135
2.924SerThr: 2.924 ± 0.073
4.09SerVal: 4.09 ± 0.083
0.373SerTrp: 0.373 ± 0.027
2.226SerTyr: 2.226 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
3.258ThrAla: 3.258 ± 0.071
0.528ThrCys: 0.528 ± 0.027
2.903ThrAsp: 2.903 ± 0.069
3.365ThrGlu: 3.365 ± 0.06
2.033ThrPhe: 2.033 ± 0.056
3.93ThrGly: 3.93 ± 0.077
0.765ThrHis: 0.765 ± 0.036
4.332ThrIle: 4.332 ± 0.084
3.621ThrLys: 3.621 ± 0.075
4.171ThrLeu: 4.171 ± 0.085
1.227ThrMet: 1.227 ± 0.041
2.436ThrAsn: 2.436 ± 0.063
1.675ThrPro: 1.675 ± 0.05
1.026ThrGln: 1.026 ± 0.037
1.59ThrArg: 1.59 ± 0.043
3.179ThrSer: 3.179 ± 0.079
2.533ThrThr: 2.533 ± 0.064
3.986ThrVal: 3.986 ± 0.081
0.28ThrTrp: 0.28 ± 0.023
1.814ThrTyr: 1.814 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
4.661ValAla: 4.661 ± 0.098
0.993ValCys: 0.993 ± 0.039
4.515ValAsp: 4.515 ± 0.078
5.908ValGlu: 5.908 ± 0.113
3.043ValPhe: 3.043 ± 0.077
4.633ValGly: 4.633 ± 0.104
0.933ValHis: 0.933 ± 0.037
5.838ValIle: 5.838 ± 0.095
5.66ValLys: 5.66 ± 0.089
6.107ValLeu: 6.107 ± 0.113
1.976ValMet: 1.976 ± 0.047
3.577ValAsn: 3.577 ± 0.08
2.011ValPro: 2.011 ± 0.056
1.374ValGln: 1.374 ± 0.053
2.338ValArg: 2.338 ± 0.058
4.553ValSer: 4.553 ± 0.086
3.353ValThr: 3.353 ± 0.071
5.517ValVal: 5.517 ± 0.103
0.343ValTrp: 0.343 ± 0.026
2.619ValTyr: 2.619 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.383TrpAla: 0.383 ± 0.022
0.069TrpCys: 0.069 ± 0.011
0.376TrpAsp: 0.376 ± 0.023
0.418TrpGlu: 0.418 ± 0.025
0.249TrpPhe: 0.249 ± 0.021
0.408TrpGly: 0.408 ± 0.024
0.091TrpHis: 0.091 ± 0.011
0.494TrpIle: 0.494 ± 0.031
0.461TrpLys: 0.461 ± 0.028
0.529TrpLeu: 0.529 ± 0.028
0.182TrpMet: 0.182 ± 0.016
0.391TrpAsn: 0.391 ± 0.026
0.11TrpPro: 0.11 ± 0.013
0.154TrpGln: 0.154 ± 0.014
0.244TrpArg: 0.244 ± 0.019
0.309TrpSer: 0.309 ± 0.021
0.272TrpThr: 0.272 ± 0.021
0.358TrpVal: 0.358 ± 0.024
0.053TrpTrp: 0.053 ± 0.008
0.251TrpTyr: 0.251 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.145TyrAla: 2.145 ± 0.061
0.538TyrCys: 0.538 ± 0.029
2.511TyrAsp: 2.511 ± 0.071
2.827TyrGlu: 2.827 ± 0.073
1.79TyrPhe: 1.79 ± 0.052
2.543TyrGly: 2.543 ± 0.053
0.597TyrHis: 0.597 ± 0.031
3.525TyrIle: 3.525 ± 0.09
3.489TyrLys: 3.489 ± 0.08
3.239TyrLeu: 3.239 ± 0.076
1.124TyrMet: 1.124 ± 0.042
2.314TyrAsn: 2.314 ± 0.068
1.217TyrPro: 1.217 ± 0.042
0.826TyrGln: 0.826 ± 0.035
1.568TyrArg: 1.568 ± 0.051
2.585TyrSer: 2.585 ± 0.068
2.023TyrThr: 2.023 ± 0.054
2.377TyrVal: 2.377 ± 0.06
0.262TyrTrp: 0.262 ± 0.021
1.717TyrTyr: 1.717 ± 0.059
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2271 proteins (693674 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski