Anaerofustis stercorihominis DSM 17244
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2331 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B1C8C3|B1C8C3_9FIRM Stage 0 sporulation protein A homolog OS=Anaerofustis stercorihominis DSM 17244 OX=445971 GN=ANASTE_00980 PE=4 SV=1
MM1 pKa = 7.6 QITKK5 pKa = 10.78 DD6 pKa = 3.61 MGIMDD11 pKa = 4.93 IVNKK15 pKa = 10.0 YY16 pKa = 7.97 PQAVSVFQAYY26 pKa = 10.33 GMGCIGCMAARR37 pKa = 11.84 FEE39 pKa = 4.3 TLEE42 pKa = 4.27 EE43 pKa = 4.08 GANAHH48 pKa = 6.93 GINVDD53 pKa = 4.75 DD54 pKa = 6.2 LVDD57 pKa = 4.48 DD58 pKa = 4.45 LNEE61 pKa = 4.43 NII63 pKa = 4.9
Molecular weight: 6.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 4.037
IPC_protein 3.91
Toseland 3.719
ProMoST 4.088
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.834
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.037
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|B1CBX8|B1CBX8_9FIRM N-acetylglucosaminylphosphatidylinositol deacetylase OS=Anaerofustis stercorihominis DSM 17244 OX=445971 GN=ANASTE_01477 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNHH10 pKa = 5.77 KK11 pKa = 10.26 RR12 pKa = 11.84 KK13 pKa = 8.97 KK14 pKa = 8.7 NHH16 pKa = 5.25 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSTPGGKK28 pKa = 9.36 NVIRR32 pKa = 11.84 NRR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.89 KK37 pKa = 10.47 GRR39 pKa = 11.84 KK40 pKa = 8.05 QLSAA44 pKa = 3.9
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.676
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.349
Sillero 12.866
Patrickios 12.398
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2331
0
2331
660854
39
3037
283.5
31.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.631 ± 0.054
1.249 ± 0.024
6.286 ± 0.047
7.267 ± 0.06
4.428 ± 0.046
6.456 ± 0.057
1.418 ± 0.023
9.382 ± 0.073
9.574 ± 0.059
8.693 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.778 ± 0.027
6.217 ± 0.049
2.69 ± 0.028
1.875 ± 0.022
3.193 ± 0.037
6.405 ± 0.049
5.096 ± 0.045
6.509 ± 0.045
0.524 ± 0.014
4.33 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here