Cyanophage S-TIM5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Aurunvirus; Synechococcus virus STIM5

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6WG04|H6WG04_9CAUD Uncharacterized protein OS=Cyanophage S-TIM5 OX=1137745 PE=4 SV=1
MM1 pKa = 8.02LEE3 pKa = 4.55LLLNLNMTCQQADD16 pKa = 3.57ALILKK21 pKa = 9.15INKK24 pKa = 9.31YY25 pKa = 10.2FDD27 pKa = 3.54LSADD31 pKa = 3.34VRR33 pKa = 11.84LEE35 pKa = 3.96LVDD38 pKa = 3.65TVKK41 pKa = 10.89DD42 pKa = 3.74YY43 pKa = 11.2TPEE46 pKa = 4.22CEE48 pKa = 4.98FYY50 pKa = 10.67WDD52 pKa = 4.37ANDD55 pKa = 3.39

Molecular weight: 6.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6WG35|H6WG35_9CAUD Uncharacterized protein OS=Cyanophage S-TIM5 OX=1137745 PE=4 SV=1
MM1 pKa = 7.11TGVYY5 pKa = 8.76STGTLRR11 pKa = 11.84LSAVTGPPWASSQKK25 pKa = 10.49KK26 pKa = 8.57VGKK29 pKa = 9.63GVKK32 pKa = 8.79TGTGVYY38 pKa = 7.67FTGSLRR44 pKa = 11.84QRR46 pKa = 11.84KK47 pKa = 6.44WEE49 pKa = 4.01RR50 pKa = 11.84GINTT54 pKa = 3.78

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

179

1

180

51181

23

1830

284.3

31.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.038 ± 0.195

0.852 ± 0.068

6.125 ± 0.13

6.487 ± 0.322

4.213 ± 0.126

8.204 ± 0.315

1.389 ± 0.091

6.291 ± 0.145

5.932 ± 0.34

7.755 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.741 ± 0.137

6.016 ± 0.259

4.058 ± 0.161

3.484 ± 0.094

4.271 ± 0.144

7.876 ± 0.167

6.96 ± 0.325

6.16 ± 0.137

1.27 ± 0.085

3.878 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski