Amino acid dipepetide frequency for Cyanophage S-TIM5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.49AlaAla: 5.49 ± 0.437
0.43AlaCys: 0.43 ± 0.089
4.259AlaAsp: 4.259 ± 0.347
3.869AlaGlu: 3.869 ± 0.386
2.755AlaPhe: 2.755 ± 0.206
6.428AlaGly: 6.428 ± 0.448
0.899AlaHis: 0.899 ± 0.123
4.553AlaIle: 4.553 ± 0.34
3.947AlaLys: 3.947 ± 0.338
5.803AlaLeu: 5.803 ± 0.326
1.172AlaMet: 1.172 ± 0.181
4.24AlaAsn: 4.24 ± 0.344
3.165AlaPro: 3.165 ± 0.266
2.442AlaGln: 2.442 ± 0.223
2.521AlaArg: 2.521 ± 0.23
5.627AlaSer: 5.627 ± 0.405
4.728AlaThr: 4.728 ± 0.346
4.201AlaVal: 4.201 ± 0.305
0.723AlaTrp: 0.723 ± 0.134
3.126AlaTyr: 3.126 ± 0.189
0.0AlaXaa: 0.0 ± 0.0
Cys
0.606CysAla: 0.606 ± 0.124
0.078CysCys: 0.078 ± 0.04
0.508CysAsp: 0.508 ± 0.099
0.684CysGlu: 0.684 ± 0.129
0.352CysPhe: 0.352 ± 0.067
0.606CysGly: 0.606 ± 0.105
0.215CysHis: 0.215 ± 0.079
0.352CysIle: 0.352 ± 0.081
0.547CysLys: 0.547 ± 0.093
0.528CysLeu: 0.528 ± 0.125
0.098CysMet: 0.098 ± 0.057
0.547CysAsn: 0.547 ± 0.112
0.508CysPro: 0.508 ± 0.108
0.254CysGln: 0.254 ± 0.065
0.293CysArg: 0.293 ± 0.081
0.86CysSer: 0.86 ± 0.128
0.391CysThr: 0.391 ± 0.082
0.528CysVal: 0.528 ± 0.131
0.137CysTrp: 0.137 ± 0.054
0.43CysTyr: 0.43 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
4.24AspAla: 4.24 ± 0.27
0.645AspCys: 0.645 ± 0.128
3.439AspAsp: 3.439 ± 0.305
3.712AspGlu: 3.712 ± 0.293
3.068AspPhe: 3.068 ± 0.264
6.174AspGly: 6.174 ± 0.553
0.938AspHis: 0.938 ± 0.137
3.927AspIle: 3.927 ± 0.285
3.341AspLys: 3.341 ± 0.293
4.728AspLeu: 4.728 ± 0.298
1.153AspMet: 1.153 ± 0.172
3.4AspAsn: 3.4 ± 0.261
3.185AspPro: 3.185 ± 0.258
1.954AspGln: 1.954 ± 0.186
2.364AspArg: 2.364 ± 0.211
4.162AspSer: 4.162 ± 0.308
3.615AspThr: 3.615 ± 0.262
3.654AspVal: 3.654 ± 0.233
0.918AspTrp: 0.918 ± 0.157
2.638AspTyr: 2.638 ± 0.21
0.0AspXaa: 0.0 ± 0.0
Glu
3.986GluAla: 3.986 ± 0.399
0.762GluCys: 0.762 ± 0.137
3.654GluAsp: 3.654 ± 0.332
6.526GluGlu: 6.526 ± 0.837
2.989GluPhe: 2.989 ± 0.308
4.435GluGly: 4.435 ± 0.326
0.801GluHis: 0.801 ± 0.113
4.513GluIle: 4.513 ± 0.385
4.299GluLys: 4.299 ± 0.406
5.881GluLeu: 5.881 ± 0.424
1.407GluMet: 1.407 ± 0.197
3.029GluAsn: 3.029 ± 0.238
1.895GluPro: 1.895 ± 0.202
2.091GluGln: 2.091 ± 0.211
2.833GluArg: 2.833 ± 0.245
4.299GluSer: 4.299 ± 0.284
3.791GluThr: 3.791 ± 0.329
4.142GluVal: 4.142 ± 0.382
1.192GluTrp: 1.192 ± 0.185
2.345GluTyr: 2.345 ± 0.188
0.0GluXaa: 0.0 ± 0.0
Phe
2.755PheAla: 2.755 ± 0.2
0.371PheCys: 0.371 ± 0.096
2.989PheAsp: 2.989 ± 0.26
2.403PheGlu: 2.403 ± 0.213
1.915PhePhe: 1.915 ± 0.251
3.693PheGly: 3.693 ± 0.367
0.606PheHis: 0.606 ± 0.121
2.775PheIle: 2.775 ± 0.257
2.56PheLys: 2.56 ± 0.253
2.95PheLeu: 2.95 ± 0.276
0.821PheMet: 0.821 ± 0.122
2.599PheAsn: 2.599 ± 0.256
1.465PhePro: 1.465 ± 0.224
1.172PheGln: 1.172 ± 0.166
1.876PheArg: 1.876 ± 0.24
3.654PheSer: 3.654 ± 0.268
3.009PheThr: 3.009 ± 0.236
2.716PheVal: 2.716 ± 0.262
0.41PheTrp: 0.41 ± 0.079
1.387PheTyr: 1.387 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
6.077GlyAla: 6.077 ± 0.471
0.586GlyCys: 0.586 ± 0.138
4.631GlyAsp: 4.631 ± 0.272
4.084GlyGlu: 4.084 ± 0.244
2.794GlyPhe: 2.794 ± 0.235
7.659GlyGly: 7.659 ± 0.657
1.29GlyHis: 1.29 ± 0.162
5.295GlyIle: 5.295 ± 0.519
4.22GlyLys: 4.22 ± 0.31
6.038GlyLeu: 6.038 ± 0.503
1.27GlyMet: 1.27 ± 0.202
4.924GlyAsn: 4.924 ± 0.484
2.071GlyPro: 2.071 ± 0.219
2.521GlyGln: 2.521 ± 0.214
3.439GlyArg: 3.439 ± 0.369
7.816GlySer: 7.816 ± 0.572
7.894GlyThr: 7.894 ± 0.889
5.354GlyVal: 5.354 ± 0.316
1.153GlyTrp: 1.153 ± 0.152
2.931GlyTyr: 2.931 ± 0.237
0.0GlyXaa: 0.0 ± 0.0
His
0.742HisAla: 0.742 ± 0.121
0.195HisCys: 0.195 ± 0.057
0.996HisAsp: 0.996 ± 0.136
0.742HisGlu: 0.742 ± 0.119
0.782HisPhe: 0.782 ± 0.132
1.016HisGly: 1.016 ± 0.162
0.215HisHis: 0.215 ± 0.068
1.094HisIle: 1.094 ± 0.176
0.86HisLys: 0.86 ± 0.168
1.114HisLeu: 1.114 ± 0.199
0.332HisMet: 0.332 ± 0.07
0.684HisAsn: 0.684 ± 0.118
0.762HisPro: 0.762 ± 0.127
0.332HisGln: 0.332 ± 0.094
0.645HisArg: 0.645 ± 0.097
0.957HisSer: 0.957 ± 0.142
0.899HisThr: 0.899 ± 0.135
0.782HisVal: 0.782 ± 0.117
0.156HisTrp: 0.156 ± 0.07
0.586HisTyr: 0.586 ± 0.098
0.0HisXaa: 0.0 ± 0.0
Ile
4.709IleAla: 4.709 ± 0.348
0.567IleCys: 0.567 ± 0.123
4.67IleAsp: 4.67 ± 0.293
4.045IleGlu: 4.045 ± 0.304
2.755IlePhe: 2.755 ± 0.24
4.924IleGly: 4.924 ± 0.364
0.703IleHis: 0.703 ± 0.12
3.243IleIle: 3.243 ± 0.229
3.869IleLys: 3.869 ± 0.321
4.709IleLeu: 4.709 ± 0.287
0.801IleMet: 0.801 ± 0.122
4.396IleAsn: 4.396 ± 0.271
2.306IlePro: 2.306 ± 0.291
2.149IleGln: 2.149 ± 0.208
3.537IleArg: 3.537 ± 0.324
4.846IleSer: 4.846 ± 0.354
4.396IleThr: 4.396 ± 0.322
3.439IleVal: 3.439 ± 0.236
0.547IleTrp: 0.547 ± 0.116
2.306IleTyr: 2.306 ± 0.17
0.0IleXaa: 0.0 ± 0.0
Lys
3.634LysAla: 3.634 ± 0.344
0.742LysCys: 0.742 ± 0.164
3.888LysAsp: 3.888 ± 0.349
4.65LysGlu: 4.65 ± 0.49
2.501LysPhe: 2.501 ± 0.284
3.146LysGly: 3.146 ± 0.31
0.723LysHis: 0.723 ± 0.158
4.65LysIle: 4.65 ± 0.394
4.807LysLys: 4.807 ± 0.492
5.158LysLeu: 5.158 ± 0.389
1.524LysMet: 1.524 ± 0.204
3.029LysAsn: 3.029 ± 0.243
2.013LysPro: 2.013 ± 0.21
2.071LysGln: 2.071 ± 0.208
2.481LysArg: 2.481 ± 0.28
3.81LysSer: 3.81 ± 0.333
3.048LysThr: 3.048 ± 0.274
4.142LysVal: 4.142 ± 0.314
1.172LysTrp: 1.172 ± 0.187
2.13LysTyr: 2.13 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
4.885LeuAla: 4.885 ± 0.318
0.625LeuCys: 0.625 ± 0.127
4.553LeuAsp: 4.553 ± 0.246
5.471LeuGlu: 5.471 ± 0.425
2.931LeuPhe: 2.931 ± 0.224
6.116LeuGly: 6.116 ± 0.462
1.27LeuHis: 1.27 ± 0.153
3.791LeuIle: 3.791 ± 0.359
5.119LeuLys: 5.119 ± 0.338
5.569LeuLeu: 5.569 ± 0.446
1.27LeuMet: 1.27 ± 0.156
5.021LeuAsn: 5.021 ± 0.327
3.537LeuPro: 3.537 ± 0.36
3.322LeuGln: 3.322 ± 0.239
4.455LeuArg: 4.455 ± 0.381
5.901LeuSer: 5.901 ± 0.417
5.393LeuThr: 5.393 ± 0.354
4.318LeuVal: 4.318 ± 0.316
0.782LeuTrp: 0.782 ± 0.138
3.224LeuTyr: 3.224 ± 0.285
0.0LeuXaa: 0.0 ± 0.0
Met
1.602MetAla: 1.602 ± 0.182
0.137MetCys: 0.137 ± 0.055
0.879MetAsp: 0.879 ± 0.143
1.25MetGlu: 1.25 ± 0.195
0.469MetPhe: 0.469 ± 0.109
1.465MetGly: 1.465 ± 0.167
0.313MetHis: 0.313 ± 0.098
1.094MetIle: 1.094 ± 0.147
1.329MetLys: 1.329 ± 0.216
1.25MetLeu: 1.25 ± 0.165
0.391MetMet: 0.391 ± 0.09
0.899MetAsn: 0.899 ± 0.138
0.879MetPro: 0.879 ± 0.17
0.391MetGln: 0.391 ± 0.086
0.762MetArg: 0.762 ± 0.154
1.29MetSer: 1.29 ± 0.164
1.348MetThr: 1.348 ± 0.184
0.957MetVal: 0.957 ± 0.177
0.313MetTrp: 0.313 ± 0.082
0.391MetTyr: 0.391 ± 0.087
0.0MetXaa: 0.0 ± 0.0
Asn
4.455AsnAla: 4.455 ± 0.335
0.528AsnCys: 0.528 ± 0.116
3.087AsnAsp: 3.087 ± 0.289
3.165AsnGlu: 3.165 ± 0.257
2.911AsnPhe: 2.911 ± 0.261
4.631AsnGly: 4.631 ± 0.435
0.996AsnHis: 0.996 ± 0.151
4.045AsnIle: 4.045 ± 0.24
3.204AsnLys: 3.204 ± 0.321
4.631AsnLeu: 4.631 ± 0.373
0.625AsnMet: 0.625 ± 0.107
4.846AsnAsn: 4.846 ± 0.52
3.165AsnPro: 3.165 ± 0.322
2.071AsnGln: 2.071 ± 0.205
2.325AsnArg: 2.325 ± 0.18
4.728AsnSer: 4.728 ± 0.435
4.377AsnThr: 4.377 ± 0.373
3.4AsnVal: 3.4 ± 0.308
0.586AsnTrp: 0.586 ± 0.103
2.384AsnTyr: 2.384 ± 0.193
0.0AsnXaa: 0.0 ± 0.0
Pro
3.068ProAla: 3.068 ± 0.24
0.332ProCys: 0.332 ± 0.093
2.442ProAsp: 2.442 ± 0.226
3.654ProGlu: 3.654 ± 0.327
1.544ProPhe: 1.544 ± 0.182
2.931ProGly: 2.931 ± 0.244
0.664ProHis: 0.664 ± 0.124
2.208ProIle: 2.208 ± 0.261
2.325ProLys: 2.325 ± 0.279
3.048ProLeu: 3.048 ± 0.308
0.625ProMet: 0.625 ± 0.12
1.915ProAsn: 1.915 ± 0.214
1.544ProPro: 1.544 ± 0.244
1.485ProGln: 1.485 ± 0.193
1.446ProArg: 1.446 ± 0.192
3.751ProSer: 3.751 ± 0.36
3.087ProThr: 3.087 ± 0.361
2.481ProVal: 2.481 ± 0.26
0.43ProTrp: 0.43 ± 0.095
1.602ProTyr: 1.602 ± 0.19
0.0ProXaa: 0.0 ± 0.0
Gln
2.325GlnAla: 2.325 ± 0.197
0.215GlnCys: 0.215 ± 0.088
2.013GlnAsp: 2.013 ± 0.194
2.208GlnGlu: 2.208 ± 0.212
1.524GlnPhe: 1.524 ± 0.141
2.911GlnGly: 2.911 ± 0.225
0.41GlnHis: 0.41 ± 0.102
2.56GlnIle: 2.56 ± 0.211
2.052GlnLys: 2.052 ± 0.165
3.146GlnLeu: 3.146 ± 0.32
0.762GlnMet: 0.762 ± 0.136
2.442GlnAsn: 2.442 ± 0.217
1.192GlnPro: 1.192 ± 0.179
1.387GlnGln: 1.387 ± 0.161
1.485GlnArg: 1.485 ± 0.189
2.306GlnSer: 2.306 ± 0.193
1.934GlnThr: 1.934 ± 0.257
2.208GlnVal: 2.208 ± 0.234
0.488GlnTrp: 0.488 ± 0.093
1.27GlnTyr: 1.27 ± 0.174
0.0GlnXaa: 0.0 ± 0.0
Arg
3.009ArgAla: 3.009 ± 0.226
0.274ArgCys: 0.274 ± 0.067
2.677ArgAsp: 2.677 ± 0.259
2.54ArgGlu: 2.54 ± 0.271
2.091ArgPhe: 2.091 ± 0.243
3.165ArgGly: 3.165 ± 0.295
0.449ArgHis: 0.449 ± 0.096
3.322ArgIle: 3.322 ± 0.36
2.735ArgLys: 2.735 ± 0.266
3.732ArgLeu: 3.732 ± 0.384
1.075ArgMet: 1.075 ± 0.16
2.013ArgAsn: 2.013 ± 0.169
1.348ArgPro: 1.348 ± 0.16
1.934ArgGln: 1.934 ± 0.196
1.895ArgArg: 1.895 ± 0.228
2.97ArgSer: 2.97 ± 0.21
2.481ArgThr: 2.481 ± 0.259
2.618ArgVal: 2.618 ± 0.237
0.586ArgTrp: 0.586 ± 0.111
1.798ArgTyr: 1.798 ± 0.173
0.0ArgXaa: 0.0 ± 0.0
Ser
5.354SerAla: 5.354 ± 0.392
0.606SerCys: 0.606 ± 0.116
4.807SerAsp: 4.807 ± 0.371
4.396SerGlu: 4.396 ± 0.325
3.224SerPhe: 3.224 ± 0.241
7.464SerGly: 7.464 ± 0.497
0.84SerHis: 0.84 ± 0.152
4.807SerIle: 4.807 ± 0.318
3.849SerLys: 3.849 ± 0.322
5.979SerLeu: 5.979 ± 0.416
1.133SerMet: 1.133 ± 0.17
5.041SerAsn: 5.041 ± 0.451
3.341SerPro: 3.341 ± 0.317
2.95SerGln: 2.95 ± 0.3
2.853SerArg: 2.853 ± 0.237
6.917SerSer: 6.917 ± 0.574
6.409SerThr: 6.409 ± 0.544
4.846SerVal: 4.846 ± 0.337
0.957SerTrp: 0.957 ± 0.135
2.989SerTyr: 2.989 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
5.354ThrAla: 5.354 ± 0.506
0.371ThrCys: 0.371 ± 0.094
4.513ThrAsp: 4.513 ± 0.382
4.259ThrGlu: 4.259 ± 0.288
2.696ThrPhe: 2.696 ± 0.254
6.487ThrGly: 6.487 ± 0.687
0.723ThrHis: 0.723 ± 0.125
3.693ThrIle: 3.693 ± 0.356
3.283ThrLys: 3.283 ± 0.264
5.061ThrLeu: 5.061 ± 0.289
0.86ThrMet: 0.86 ± 0.128
4.416ThrAsn: 4.416 ± 0.45
3.478ThrPro: 3.478 ± 0.347
2.735ThrGln: 2.735 ± 0.268
2.227ThrArg: 2.227 ± 0.189
6.272ThrSer: 6.272 ± 0.482
5.275ThrThr: 5.275 ± 0.549
4.435ThrVal: 4.435 ± 0.376
1.075ThrTrp: 1.075 ± 0.153
2.384ThrTyr: 2.384 ± 0.268
0.0ThrXaa: 0.0 ± 0.0
Val
4.553ValAla: 4.553 ± 0.332
0.488ValCys: 0.488 ± 0.136
4.103ValAsp: 4.103 ± 0.33
3.732ValGlu: 3.732 ± 0.297
2.716ValPhe: 2.716 ± 0.241
5.178ValGly: 5.178 ± 0.432
1.094ValHis: 1.094 ± 0.176
3.341ValIle: 3.341 ± 0.236
3.361ValLys: 3.361 ± 0.275
4.084ValLeu: 4.084 ± 0.335
0.938ValMet: 0.938 ± 0.148
3.478ValAsn: 3.478 ± 0.297
2.95ValPro: 2.95 ± 0.246
1.778ValGln: 1.778 ± 0.175
2.95ValArg: 2.95 ± 0.297
5.021ValSer: 5.021 ± 0.365
4.689ValThr: 4.689 ± 0.42
4.162ValVal: 4.162 ± 0.312
0.488ValTrp: 0.488 ± 0.106
2.501ValTyr: 2.501 ± 0.261
0.0ValXaa: 0.0 ± 0.0
Trp
0.703TrpAla: 0.703 ± 0.124
0.195TrpCys: 0.195 ± 0.091
0.938TrpAsp: 0.938 ± 0.15
1.211TrpGlu: 1.211 ± 0.183
0.528TrpPhe: 0.528 ± 0.113
0.918TrpGly: 0.918 ± 0.145
0.156TrpHis: 0.156 ± 0.057
0.84TrpIle: 0.84 ± 0.136
1.211TrpLys: 1.211 ± 0.2
1.153TrpLeu: 1.153 ± 0.176
0.293TrpMet: 0.293 ± 0.074
0.547TrpAsn: 0.547 ± 0.099
0.274TrpPro: 0.274 ± 0.071
0.41TrpGln: 0.41 ± 0.083
0.684TrpArg: 0.684 ± 0.121
0.703TrpSer: 0.703 ± 0.14
0.586TrpThr: 0.586 ± 0.115
0.821TrpVal: 0.821 ± 0.128
0.117TrpTrp: 0.117 ± 0.052
0.391TrpTyr: 0.391 ± 0.087
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.833TyrAla: 2.833 ± 0.225
0.371TyrCys: 0.371 ± 0.099
2.208TyrAsp: 2.208 ± 0.183
2.267TyrGlu: 2.267 ± 0.224
1.583TyrPhe: 1.583 ± 0.177
2.696TyrGly: 2.696 ± 0.246
0.586TyrHis: 0.586 ± 0.116
2.814TyrIle: 2.814 ± 0.227
2.403TyrLys: 2.403 ± 0.364
2.989TyrLeu: 2.989 ± 0.272
0.84TyrMet: 0.84 ± 0.12
2.696TyrAsn: 2.696 ± 0.278
1.504TyrPro: 1.504 ± 0.181
1.387TyrGln: 1.387 ± 0.173
1.641TyrArg: 1.641 ± 0.152
2.872TyrSer: 2.872 ± 0.265
2.247TyrThr: 2.247 ± 0.209
2.403TyrVal: 2.403 ± 0.229
0.469TyrTrp: 0.469 ± 0.099
1.973TyrTyr: 1.973 ± 0.198
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 180 proteins (51181 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski