Roseburia hominis (strain DSM 16839 / JCM 17582 / NCIMB 14029 / A2-183)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; Roseburia hominis

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3351 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2SY23|G2SY23_ROSHA ABC transporter (ATP-binding protein) OS=Roseburia hominis (strain DSM 16839 / JCM 17582 / NCIMB 14029 / A2-183) OX=585394 GN=RHOM_11705 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.06LKK4 pKa = 10.54KK5 pKa = 10.35VLAVSLAAAMTLSMAACGGSSSDD28 pKa = 3.87SSAASDD34 pKa = 3.68DD35 pKa = 3.65ATTGSVAATTTEE47 pKa = 4.0SGDD50 pKa = 3.6AAEE53 pKa = 4.56TTDD56 pKa = 3.73GALNYY61 pKa = 10.76ASIKK65 pKa = 10.5LGEE68 pKa = 5.17DD69 pKa = 3.3YY70 pKa = 11.32TDD72 pKa = 2.87ITTTIHH78 pKa = 6.17VFNQRR83 pKa = 11.84TDD85 pKa = 3.26MSEE88 pKa = 3.54DD89 pKa = 3.56SYY91 pKa = 11.57PGKK94 pKa = 10.31NWEE97 pKa = 4.48AYY99 pKa = 9.05IADD102 pKa = 4.22FNEE105 pKa = 4.38MYY107 pKa = 10.71PNITVEE113 pKa = 4.43VEE115 pKa = 3.44TDD117 pKa = 3.07TNYY120 pKa = 10.76SDD122 pKa = 6.0DD123 pKa = 3.7SLLRR127 pKa = 11.84LQSGDD132 pKa = 2.86WGDD135 pKa = 3.23IMMIPAVDD143 pKa = 3.5KK144 pKa = 11.32ADD146 pKa = 3.49LSEE149 pKa = 4.19YY150 pKa = 9.82FLPYY154 pKa = 9.0GTLDD158 pKa = 3.41EE159 pKa = 4.81MEE161 pKa = 4.39GQIKK165 pKa = 9.27FANTWDD171 pKa = 3.94YY172 pKa = 11.88DD173 pKa = 3.68GLVYY177 pKa = 10.31GVPSTGNAQGIVYY190 pKa = 9.49NKK192 pKa = 10.26RR193 pKa = 11.84VFTEE197 pKa = 4.0AGVAEE202 pKa = 4.62TPKK205 pKa = 10.7TPDD208 pKa = 2.91EE209 pKa = 5.0FIAALQAIKK218 pKa = 10.43DD219 pKa = 4.06YY220 pKa = 10.78DD221 pKa = 3.75SSIIPLYY228 pKa = 10.24TNYY231 pKa = 10.84ADD233 pKa = 3.82GWPMEE238 pKa = 4.09QWDD241 pKa = 4.14GQIAGTTTGDD251 pKa = 3.32STYY254 pKa = 10.5MNQKK258 pKa = 9.84LLHH261 pKa = 6.26TKK263 pKa = 10.61DD264 pKa = 3.36PFQDD268 pKa = 3.74YY269 pKa = 11.3GDD271 pKa = 3.78NTHH274 pKa = 7.41PYY276 pKa = 10.27ALYY279 pKa = 10.2KK280 pKa = 10.38VLYY283 pKa = 10.04DD284 pKa = 3.94AVADD288 pKa = 4.38GLTEE292 pKa = 5.36DD293 pKa = 5.66DD294 pKa = 4.06FTTTSWEE301 pKa = 4.14DD302 pKa = 3.16SKK304 pKa = 12.06GMINRR309 pKa = 11.84GEE311 pKa = 4.15IATMVLGSWAYY322 pKa = 10.91SQMVDD327 pKa = 3.46ADD329 pKa = 3.87SHH331 pKa = 7.73GEE333 pKa = 4.08DD334 pKa = 3.0IGYY337 pKa = 8.25MPFPITIDD345 pKa = 3.25GKK347 pKa = 10.34QYY349 pKa = 11.15ASAGADD355 pKa = 3.13YY356 pKa = 11.33SFGINAASDD365 pKa = 3.82ADD367 pKa = 3.85HH368 pKa = 6.37QAAAMVFVKK377 pKa = 9.95WMTEE381 pKa = 3.79EE382 pKa = 4.31SGFAYY387 pKa = 10.57NEE389 pKa = 3.9GGIPIAADD397 pKa = 3.57DD398 pKa = 4.15NDD400 pKa = 4.04YY401 pKa = 11.09PDD403 pKa = 4.76AYY405 pKa = 10.8AEE407 pKa = 4.28FGDD410 pKa = 3.76VTFVSDD416 pKa = 3.76EE417 pKa = 4.15SALEE421 pKa = 4.12GEE423 pKa = 4.37EE424 pKa = 4.94DD425 pKa = 5.06LLNALNADD433 pKa = 3.35SGLNINAGGKK443 pKa = 9.74EE444 pKa = 3.9KK445 pKa = 10.41VQEE448 pKa = 4.48IIEE451 pKa = 4.34HH452 pKa = 6.44ASNGDD457 pKa = 3.3MSYY460 pKa = 11.58DD461 pKa = 4.33DD462 pKa = 6.15IMAEE466 pKa = 4.17WNEE469 pKa = 4.0KK470 pKa = 6.58WTQAQEE476 pKa = 4.41DD477 pKa = 3.91NGVTAEE483 pKa = 3.93

Molecular weight: 52.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2SWG8|G2SWG8_ROSHA Mutator family transposase OS=Roseburia hominis (strain DSM 16839 / JCM 17582 / NCIMB 14029 / A2-183) OX=585394 GN=RHOM_05665 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3351

0

3351

1052490

30

2665

314.1

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.25 ± 0.046

1.52 ± 0.018

5.732 ± 0.036

7.862 ± 0.052

3.903 ± 0.028

7.098 ± 0.039

1.822 ± 0.022

6.843 ± 0.041

6.17 ± 0.041

8.877 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.173 ± 0.024

4.119 ± 0.031

3.21 ± 0.025

3.441 ± 0.027

4.804 ± 0.038

5.515 ± 0.034

5.522 ± 0.041

6.981 ± 0.034

0.921 ± 0.014

4.236 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski