Amino acid dipepetide frequency for Roseburia hominis (strain DSM 16839 / JCM 17582 / NCIMB 14029 / A2-183)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.986AlaAla: 8.986 ± 0.137	1.185AlaCys: 1.185 ± 0.037	5.435AlaAsp: 5.435 ± 0.087	6.573AlaGlu: 6.573 ± 0.101	3.134AlaPhe: 3.134 ± 0.062	6.971AlaGly: 6.971 ± 0.093	1.273AlaHis: 1.273 ± 0.038	5.001AlaIle: 5.001 ± 0.068	4.848AlaLys: 4.848 ± 0.079	7.257AlaLeu: 7.257 ± 0.094	2.606AlaMet: 2.606 ± 0.055	2.705AlaAsn: 2.705 ± 0.053	2.195AlaPro: 2.195 ± 0.046	2.81AlaGln: 2.81 ± 0.06	3.298AlaArg: 3.298 ± 0.064	3.999AlaSer: 3.999 ± 0.07	3.534AlaThr: 3.534 ± 0.07	6.837AlaVal: 6.837 ± 0.101	0.67AlaTrp: 0.67 ± 0.026	3.187AlaTyr: 3.187 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	1.153CysAla: 1.153 ± 0.035	0.297CysCys: 0.297 ± 0.019	0.825CysAsp: 0.825 ± 0.029	0.983CysGlu: 0.983 ± 0.032	0.579CysPhe: 0.579 ± 0.025	1.602CysGly: 1.602 ± 0.042	0.31CysHis: 0.31 ± 0.017	1.066CysIle: 1.066 ± 0.034	0.775CysLys: 0.775 ± 0.028	1.172CysLeu: 1.172 ± 0.036	0.51CysMet: 0.51 ± 0.025	0.577CysAsn: 0.577 ± 0.024	0.626CysPro: 0.626 ± 0.025	0.404CysGln: 0.404 ± 0.02	0.886CysArg: 0.886 ± 0.029	0.871CysSer: 0.871 ± 0.028	0.666CysThr: 0.666 ± 0.025	1.113CysVal: 1.113 ± 0.032	0.128CysTrp: 0.128 ± 0.012	0.655CysTyr: 0.655 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	5.106AspAla: 5.106 ± 0.075	0.827AspCys: 0.827 ± 0.024	3.066AspAsp: 3.066 ± 0.069	4.752AspGlu: 4.752 ± 0.079	2.629AspPhe: 2.629 ± 0.05	4.793AspGly: 4.793 ± 0.079	0.914AspHis: 0.914 ± 0.033	4.143AspIle: 4.143 ± 0.074	2.972AspLys: 2.972 ± 0.057	4.601AspLeu: 4.601 ± 0.071	1.843AspMet: 1.843 ± 0.043	2.036AspAsn: 2.036 ± 0.05	1.745AspPro: 1.745 ± 0.043	1.406AspGln: 1.406 ± 0.032	2.589AspArg: 2.589 ± 0.054	2.848AspSer: 2.848 ± 0.059	3.441AspThr: 3.441 ± 0.066	4.045AspVal: 4.045 ± 0.075	0.649AspTrp: 0.649 ± 0.026	2.916AspTyr: 2.916 ± 0.066	0.0AspXaa: 0.0 ± 0.0
Glu	6.227GluAla: 6.227 ± 0.087	0.881GluCys: 0.881 ± 0.027	4.328GluAsp: 4.328 ± 0.07	7.501GluGlu: 7.501 ± 0.12	2.368GluPhe: 2.368 ± 0.051	4.215GluGly: 4.215 ± 0.075	1.661GluHis: 1.661 ± 0.048	5.377GluIle: 5.377 ± 0.086	6.484GluLys: 6.484 ± 0.091	7.175GluLeu: 7.175 ± 0.095	2.55GluMet: 2.55 ± 0.056	4.068GluAsn: 4.068 ± 0.061	1.889GluPro: 1.889 ± 0.049	3.766GluGln: 3.766 ± 0.069	4.173GluArg: 4.173 ± 0.075	3.323GluSer: 3.323 ± 0.063	4.119GluThr: 4.119 ± 0.069	4.667GluVal: 4.667 ± 0.076	0.654GluTrp: 0.654 ± 0.024	3.195GluTyr: 3.195 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	3.334PheAla: 3.334 ± 0.062	0.786PheCys: 0.786 ± 0.031	2.485PheAsp: 2.485 ± 0.047	2.458PheGlu: 2.458 ± 0.051	1.784PhePhe: 1.784 ± 0.05	2.93PheGly: 2.93 ± 0.056	0.844PheHis: 0.844 ± 0.028	2.309PheIle: 2.309 ± 0.052	1.723PheLys: 1.723 ± 0.044	3.929PheLeu: 3.929 ± 0.076	1.138PheMet: 1.138 ± 0.035	1.313PheAsn: 1.313 ± 0.032	1.287PhePro: 1.287 ± 0.034	1.108PheGln: 1.108 ± 0.036	1.649PheArg: 1.649 ± 0.04	2.734PheSer: 2.734 ± 0.055	2.166PheThr: 2.166 ± 0.044	2.837PheVal: 2.837 ± 0.059	0.424PheTrp: 0.424 ± 0.018	1.788PheTyr: 1.788 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	5.409GlyAla: 5.409 ± 0.085	1.278GlyCys: 1.278 ± 0.033	3.552GlyAsp: 3.552 ± 0.061	5.079GlyGlu: 5.079 ± 0.067	2.889GlyPhe: 2.889 ± 0.054	4.728GlyGly: 4.728 ± 0.087	1.219GlyHis: 1.219 ± 0.033	5.905GlyIle: 5.905 ± 0.085	5.08GlyLys: 5.08 ± 0.077	5.521GlyLeu: 5.521 ± 0.078	2.733GlyMet: 2.733 ± 0.061	3.082GlyAsn: 3.082 ± 0.065	1.103GlyPro: 1.103 ± 0.033	2.194GlyGln: 2.194 ± 0.051	3.402GlyArg: 3.402 ± 0.066	4.117GlySer: 4.117 ± 0.06	4.586GlyThr: 4.586 ± 0.085	5.044GlyVal: 5.044 ± 0.083	0.744GlyTrp: 0.744 ± 0.031	3.315GlyTyr: 3.315 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.321HisAla: 1.321 ± 0.036	0.298HisCys: 0.298 ± 0.016	0.998HisAsp: 0.998 ± 0.033	1.167HisGlu: 1.167 ± 0.035	0.909HisPhe: 0.909 ± 0.028	1.272HisGly: 1.272 ± 0.034	0.431HisHis: 0.431 ± 0.021	1.39HisIle: 1.39 ± 0.038	0.882HisLys: 0.882 ± 0.035	1.578HisLeu: 1.578 ± 0.045	0.596HisMet: 0.596 ± 0.026	0.733HisAsn: 0.733 ± 0.026	0.931HisPro: 0.931 ± 0.034	0.576HisGln: 0.576 ± 0.023	0.928HisArg: 0.928 ± 0.032	0.947HisSer: 0.947 ± 0.028	1.08HisThr: 1.08 ± 0.03	1.157HisVal: 1.157 ± 0.034	0.175HisTrp: 0.175 ± 0.012	0.851HisTyr: 0.851 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.957IleAla: 5.957 ± 0.085	1.281IleCys: 1.281 ± 0.036	3.866IleAsp: 3.866 ± 0.071	4.562IleGlu: 4.562 ± 0.085	2.619IlePhe: 2.619 ± 0.054	5.027IleGly: 5.027 ± 0.076	1.282IleHis: 1.282 ± 0.037	4.277IleIle: 4.277 ± 0.079	3.482IleLys: 3.482 ± 0.07	6.594IleLeu: 6.594 ± 0.093	1.797IleMet: 1.797 ± 0.041	2.53IleAsn: 2.53 ± 0.047	2.899IlePro: 2.899 ± 0.055	2.066IleGln: 2.066 ± 0.049	3.881IleArg: 3.881 ± 0.071	4.325IleSer: 4.325 ± 0.074	3.959IleThr: 3.959 ± 0.069	4.767IleVal: 4.767 ± 0.079	0.596IleTrp: 0.596 ± 0.027	2.666IleTyr: 2.666 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	4.495LysAla: 4.495 ± 0.073	0.707LysCys: 0.707 ± 0.025	3.31LysAsp: 3.31 ± 0.07	6.037LysGlu: 6.037 ± 0.097	1.6LysPhe: 1.6 ± 0.043	3.591LysGly: 3.591 ± 0.06	0.986LysHis: 0.986 ± 0.032	4.306LysIle: 4.306 ± 0.065	5.433LysLys: 5.433 ± 0.081	4.982LysLeu: 4.982 ± 0.073	2.023LysMet: 2.023 ± 0.048	3.242LysAsn: 3.242 ± 0.061	1.897LysPro: 1.897 ± 0.041	2.376LysGln: 2.376 ± 0.055	3.159LysArg: 3.159 ± 0.057	3.151LysSer: 3.151 ± 0.058	3.368LysThr: 3.368 ± 0.057	3.932LysVal: 3.932 ± 0.069	0.537LysTrp: 0.537 ± 0.025	2.566LysTyr: 2.566 ± 0.045	0.0LysXaa: 0.0 ± 0.0
Leu	7.63LeuAla: 7.63 ± 0.088	1.59LeuCys: 1.59 ± 0.042	4.936LeuAsp: 4.936 ± 0.078	6.084LeuGlu: 6.084 ± 0.078	3.747LeuPhe: 3.747 ± 0.075	5.805LeuGly: 5.805 ± 0.082	1.666LeuHis: 1.666 ± 0.041	5.539LeuIle: 5.539 ± 0.086	5.416LeuLys: 5.416 ± 0.075	8.938LeuLeu: 8.938 ± 0.127	2.566LeuMet: 2.566 ± 0.056	3.591LeuAsn: 3.591 ± 0.059	3.496LeuPro: 3.496 ± 0.06	2.761LeuGln: 2.761 ± 0.049	3.956LeuArg: 3.956 ± 0.067	5.989LeuSer: 5.989 ± 0.077	5.153LeuThr: 5.153 ± 0.07	5.668LeuVal: 5.668 ± 0.078	0.804LeuTrp: 0.804 ± 0.028	3.439LeuTyr: 3.439 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	2.619MetAla: 2.619 ± 0.056	0.373MetCys: 0.373 ± 0.019	1.917MetAsp: 1.917 ± 0.041	2.738MetGlu: 2.738 ± 0.053	0.997MetPhe: 0.997 ± 0.031	2.076MetGly: 2.076 ± 0.047	0.518MetHis: 0.518 ± 0.022	2.089MetIle: 2.089 ± 0.048	2.329MetLys: 2.329 ± 0.049	2.854MetLeu: 2.854 ± 0.053	0.95MetMet: 0.95 ± 0.036	1.556MetAsn: 1.556 ± 0.036	1.19MetPro: 1.19 ± 0.033	1.363MetGln: 1.363 ± 0.035	1.461MetArg: 1.461 ± 0.034	1.759MetSer: 1.759 ± 0.042	1.841MetThr: 1.841 ± 0.044	1.903MetVal: 1.903 ± 0.044	0.232MetTrp: 0.232 ± 0.016	0.964MetTyr: 0.964 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.289AsnAla: 3.289 ± 0.067	0.549AsnCys: 0.549 ± 0.024	2.124AsnAsp: 2.124 ± 0.048	2.802AsnGlu: 2.802 ± 0.056	1.476AsnPhe: 1.476 ± 0.043	3.393AsnGly: 3.393 ± 0.061	0.831AsnHis: 0.831 ± 0.029	3.021AsnIle: 3.021 ± 0.055	2.02AsnLys: 2.02 ± 0.047	3.591AsnLeu: 3.591 ± 0.063	1.324AsnMet: 1.324 ± 0.037	1.676AsnAsn: 1.676 ± 0.054	1.88AsnPro: 1.88 ± 0.043	1.512AsnGln: 1.512 ± 0.038	2.093AsnArg: 2.093 ± 0.053	2.095AsnSer: 2.095 ± 0.052	2.315AsnThr: 2.315 ± 0.045	2.961AsnVal: 2.961 ± 0.067	0.383AsnTrp: 0.383 ± 0.019	1.852AsnTyr: 1.852 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	2.632ProAla: 2.632 ± 0.062	0.455ProCys: 0.455 ± 0.022	2.339ProAsp: 2.339 ± 0.047	3.392ProGlu: 3.392 ± 0.064	1.409ProPhe: 1.409 ± 0.042	2.224ProGly: 2.224 ± 0.046	0.558ProHis: 0.558 ± 0.023	1.864ProIle: 1.864 ± 0.041	1.84ProLys: 1.84 ± 0.045	2.643ProLeu: 2.643 ± 0.05	0.869ProMet: 0.869 ± 0.028	1.1ProAsn: 1.1 ± 0.039	0.707ProPro: 0.707 ± 0.03	1.207ProGln: 1.207 ± 0.039	1.038ProArg: 1.038 ± 0.034	1.565ProSer: 1.565 ± 0.039	1.494ProThr: 1.494 ± 0.046	3.005ProVal: 3.005 ± 0.049	0.325ProTrp: 0.325 ± 0.019	1.438ProTyr: 1.438 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.665GlnAla: 2.665 ± 0.057	0.388GlnCys: 0.388 ± 0.019	1.557GlnAsp: 1.557 ± 0.043	2.823GlnGlu: 2.823 ± 0.059	1.249GlnPhe: 1.249 ± 0.032	1.993GlnGly: 1.993 ± 0.042	0.508GlnHis: 0.508 ± 0.023	2.781GlnIle: 2.781 ± 0.054	2.741GlnLys: 2.741 ± 0.053	2.852GlnLeu: 2.852 ± 0.059	1.391GlnMet: 1.391 ± 0.041	1.722GlnAsn: 1.722 ± 0.052	1.0GlnPro: 1.0 ± 0.038	1.425GlnGln: 1.425 ± 0.044	1.618GlnArg: 1.618 ± 0.042	1.72GlnSer: 1.72 ± 0.042	2.112GlnThr: 2.112 ± 0.056	2.072GlnVal: 2.072 ± 0.051	0.301GlnTrp: 0.301 ± 0.017	1.495GlnTyr: 1.495 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	3.294ArgAla: 3.294 ± 0.065	0.629ArgCys: 0.629 ± 0.025	2.55ArgAsp: 2.55 ± 0.048	4.679ArgGlu: 4.679 ± 0.086	1.89ArgPhe: 1.89 ± 0.041	2.756ArgGly: 2.756 ± 0.061	0.964ArgHis: 0.964 ± 0.039	3.665ArgIle: 3.665 ± 0.064	3.416ArgLys: 3.416 ± 0.059	4.141ArgLeu: 4.141 ± 0.07	1.838ArgMet: 1.838 ± 0.044	2.01ArgAsn: 2.01 ± 0.047	1.4ArgPro: 1.4 ± 0.04	2.0ArgGln: 2.0 ± 0.054	2.775ArgArg: 2.775 ± 0.065	2.26ArgSer: 2.26 ± 0.041	2.402ArgThr: 2.402 ± 0.049	2.853ArgVal: 2.853 ± 0.052	0.422ArgTrp: 0.422 ± 0.018	2.097ArgTyr: 2.097 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	4.441SerAla: 4.441 ± 0.069	0.794SerCys: 0.794 ± 0.029	3.384SerAsp: 3.384 ± 0.064	3.82SerGlu: 3.82 ± 0.058	2.43SerPhe: 2.43 ± 0.047	4.824SerGly: 4.824 ± 0.081	1.034SerHis: 1.034 ± 0.036	3.772SerIle: 3.772 ± 0.069	2.758SerLys: 2.758 ± 0.051	4.551SerLeu: 4.551 ± 0.082	1.89SerMet: 1.89 ± 0.046	2.153SerAsn: 2.153 ± 0.047	1.534SerPro: 1.534 ± 0.038	1.741SerGln: 1.741 ± 0.046	2.767SerArg: 2.767 ± 0.059	3.246SerSer: 3.246 ± 0.068	2.732SerThr: 2.732 ± 0.057	4.209SerVal: 4.209 ± 0.074	0.524SerTrp: 0.524 ± 0.022	2.543SerTyr: 2.543 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	4.958ThrAla: 4.958 ± 0.088	0.674ThrCys: 0.674 ± 0.026	3.515ThrAsp: 3.515 ± 0.061	4.328ThrGlu: 4.328 ± 0.078	2.154ThrPhe: 2.154 ± 0.048	4.874ThrGly: 4.874 ± 0.071	0.882ThrHis: 0.882 ± 0.031	3.693ThrIle: 3.693 ± 0.069	2.859ThrLys: 2.859 ± 0.049	4.805ThrLeu: 4.805 ± 0.074	1.509ThrMet: 1.509 ± 0.041	1.95ThrAsn: 1.95 ± 0.044	2.155ThrPro: 2.155 ± 0.055	1.642ThrGln: 1.642 ± 0.032	2.188ThrArg: 2.188 ± 0.049	2.938ThrSer: 2.938 ± 0.056	2.957ThrThr: 2.957 ± 0.076	4.516ThrVal: 4.516 ± 0.073	0.435ThrTrp: 0.435 ± 0.019	2.192ThrTyr: 2.192 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	5.347ValAla: 5.347 ± 0.085	1.379ValCys: 1.379 ± 0.032	3.929ValAsp: 3.929 ± 0.068	4.762ValGlu: 4.762 ± 0.07	2.948ValPhe: 2.948 ± 0.051	4.214ValGly: 4.214 ± 0.065	1.21ValHis: 1.21 ± 0.034	4.981ValIle: 4.981 ± 0.074	3.969ValLys: 3.969 ± 0.066	6.806ValLeu: 6.806 ± 0.094	2.147ValMet: 2.147 ± 0.045	2.855ValAsn: 2.855 ± 0.055	2.534ValPro: 2.534 ± 0.054	2.028ValGln: 2.028 ± 0.045	3.331ValArg: 3.331 ± 0.061	4.509ValSer: 4.509 ± 0.071	4.462ValThr: 4.462 ± 0.087	4.85ValVal: 4.85 ± 0.08	0.727ValTrp: 0.727 ± 0.033	2.823ValTyr: 2.823 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.577TrpAla: 0.577 ± 0.026	0.155TrpCys: 0.155 ± 0.011	0.525TrpAsp: 0.525 ± 0.021	0.716TrpGlu: 0.716 ± 0.027	0.4TrpPhe: 0.4 ± 0.021	0.644TrpGly: 0.644 ± 0.025	0.19TrpHis: 0.19 ± 0.015	0.631TrpIle: 0.631 ± 0.027	0.669TrpLys: 0.669 ± 0.026	0.856TrpLeu: 0.856 ± 0.027	0.316TrpMet: 0.316 ± 0.016	0.559TrpAsn: 0.559 ± 0.024	0.19TrpPro: 0.19 ± 0.015	0.396TrpGln: 0.396 ± 0.019	0.421TrpArg: 0.421 ± 0.021	0.506TrpSer: 0.506 ± 0.022	0.43TrpThr: 0.43 ± 0.02	0.524TrpVal: 0.524 ± 0.022	0.099TrpTrp: 0.099 ± 0.01	0.404TrpTyr: 0.404 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.063TyrAla: 3.063 ± 0.06	0.674TyrCys: 0.674 ± 0.026	2.681TyrAsp: 2.681 ± 0.059	3.363TyrGlu: 3.363 ± 0.057	1.814TyrPhe: 1.814 ± 0.045	3.047TyrGly: 3.047 ± 0.06	0.941TyrHis: 0.941 ± 0.03	2.622TyrIle: 2.622 ± 0.048	2.005TyrLys: 2.005 ± 0.057	3.924TyrLeu: 3.924 ± 0.064	1.134TyrMet: 1.134 ± 0.032	1.728TyrAsn: 1.728 ± 0.038	1.444TyrPro: 1.444 ± 0.036	1.633TyrGln: 1.633 ± 0.037	2.429TyrArg: 2.429 ± 0.046	2.247TyrSer: 2.247 ± 0.048	2.404TyrThr: 2.404 ± 0.054	2.85TyrVal: 2.85 ± 0.055	0.382TyrTrp: 0.382 ± 0.019	1.971TyrTyr: 1.971 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3351 proteins (1052490 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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