Kitasatospora cheerisanensis KCTC 2395
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7804 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A066YUC6|A0A066YUC6_9ACTN MaoC family dehydratase OS=Kitasatospora cheerisanensis KCTC 2395 OX=1348663 GN=KCH_33590 PE=3 SV=1
MM1 pKa = 7.74 AGTGEE6 pKa = 4.21 ALEE9 pKa = 4.12 VWIDD13 pKa = 3.41 QDD15 pKa = 3.99 LCTGDD20 pKa = 5.01 GICAQYY26 pKa = 10.9 APEE29 pKa = 4.23 VFEE32 pKa = 5.9 LDD34 pKa = 3.05 IDD36 pKa = 3.65 GLAYY40 pKa = 10.7 VKK42 pKa = 11.14 GEE44 pKa = 4.09 DD45 pKa = 3.72 EE46 pKa = 5.0 EE47 pKa = 5.79 LRR49 pKa = 11.84 QQPGEE54 pKa = 4.23 TVPVPLTLLQDD65 pKa = 3.71 VVDD68 pKa = 4.49 SVKK71 pKa = 10.72 DD72 pKa = 3.68 CPGDD76 pKa = 4.09 CIHH79 pKa = 6.53 VRR81 pKa = 11.84 RR82 pKa = 11.84 ATDD85 pKa = 3.42 GVEE88 pKa = 4.36 VYY90 pKa = 10.91 GPDD93 pKa = 3.44 ADD95 pKa = 3.69
Molecular weight: 10.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.668
IPC_protein 3.643
Toseland 3.427
ProMoST 3.77
Dawson 3.643
Bjellqvist 3.846
Wikipedia 3.592
Rodwell 3.478
Grimsley 3.35
Solomon 3.617
Lehninger 3.579
Nozaki 3.77
DTASelect 3.986
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.77
Patrickios 0.846
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|A0A066Z238|A0A066Z238_9ACTN Amidinotransferase OS=Kitasatospora cheerisanensis KCTC 2395 OX=1348663 GN=KCH_06820 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.58 GRR40 pKa = 11.84 TALSAA45 pKa = 3.73
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7804
0
7804
2315440
37
4988
296.7
31.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.566 ± 0.048
0.838 ± 0.008
5.686 ± 0.024
5.281 ± 0.037
2.547 ± 0.015
9.756 ± 0.037
2.39 ± 0.016
2.74 ± 0.022
1.75 ± 0.021
10.664 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.543 ± 0.012
1.708 ± 0.021
6.764 ± 0.039
2.893 ± 0.019
8.662 ± 0.05
4.81 ± 0.028
5.94 ± 0.033
8.039 ± 0.031
1.527 ± 0.013
1.897 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here