Streptomyces populi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0SQW1|A0A2I0SQW1_9ACTN DUF4253 domain-containing protein OS=Streptomyces populi OX=2058924 GN=CW362_14540 PE=4 SV=1
MM1 pKa = 7.68SDD3 pKa = 4.62DD4 pKa = 4.0VLSSIPTDD12 pKa = 3.86PQWQPDD18 pKa = 3.4RR19 pKa = 11.84PAAEE23 pKa = 3.83RR24 pKa = 11.84TAALAADD31 pKa = 4.82LAPGLSDD38 pKa = 3.69GVDD41 pKa = 3.32VEE43 pKa = 5.86IDD45 pKa = 4.14VTWHH49 pKa = 7.2DD50 pKa = 3.76ALTPVDD56 pKa = 3.76CGEE59 pKa = 3.75NLEE62 pKa = 5.48RR63 pKa = 11.84IGCPHH68 pKa = 7.14CGASIDD74 pKa = 4.11LEE76 pKa = 4.49WYY78 pKa = 9.13ADD80 pKa = 3.75LVEE83 pKa = 4.44SHH85 pKa = 6.97CEE87 pKa = 3.8DD88 pKa = 3.46GFATLAVEE96 pKa = 4.82VPCCGAATSLDD107 pKa = 3.62VLDD110 pKa = 4.49YY111 pKa = 11.12AWPCGFARR119 pKa = 11.84FEE121 pKa = 3.81IAIWNPEE128 pKa = 3.89RR129 pKa = 11.84DD130 pKa = 3.34WFDD133 pKa = 4.23DD134 pKa = 3.97EE135 pKa = 4.33EE136 pKa = 4.49LATIGDD142 pKa = 4.27TLGHH146 pKa = 6.2PVKK149 pKa = 10.52QIRR152 pKa = 11.84AHH154 pKa = 5.42II155 pKa = 4.08

Molecular weight: 16.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0SIJ7|A0A2I0SIJ7_9ACTN Beta sliding clamp OS=Streptomyces populi OX=2058924 GN=CW362_28000 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8072

0

8072

2648258

25

8026

328.1

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.429 ± 0.036

0.802 ± 0.008

6.034 ± 0.022

5.7 ± 0.032

2.748 ± 0.015

9.652 ± 0.03

2.31 ± 0.013

2.985 ± 0.018

2.117 ± 0.022

10.223 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.707 ± 0.013

1.742 ± 0.015

6.125 ± 0.026

2.619 ± 0.016

8.167 ± 0.029

5.24 ± 0.021

6.219 ± 0.023

8.544 ± 0.027

1.54 ± 0.01

2.098 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski