Amino acid dipepetide frequency for Streptomyces populi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.793AlaAla: 20.793 ± 0.137	1.078AlaCys: 1.078 ± 0.023	8.366AlaAsp: 8.366 ± 0.073	8.633AlaGlu: 8.633 ± 0.092	3.573AlaPhe: 3.573 ± 0.041	13.177AlaGly: 13.177 ± 0.084	2.869AlaHis: 2.869 ± 0.035	3.096AlaIle: 3.096 ± 0.042	2.747AlaLys: 2.747 ± 0.042	14.379AlaLeu: 14.379 ± 0.111	2.383AlaMet: 2.383 ± 0.029	1.859AlaAsn: 1.859 ± 0.031	7.217AlaPro: 7.217 ± 0.079	3.486AlaGln: 3.486 ± 0.045	10.48AlaArg: 10.48 ± 0.09	6.277AlaSer: 6.277 ± 0.054	6.843AlaThr: 6.843 ± 0.062	12.346AlaVal: 12.346 ± 0.09	1.891AlaTrp: 1.891 ± 0.025	2.798AlaTyr: 2.798 ± 0.03	0.0AlaXaa: 0.0 ± 0.0
Cys	1.077CysAla: 1.077 ± 0.023	0.101CysCys: 0.101 ± 0.006	0.46CysAsp: 0.46 ± 0.015	0.403CysGlu: 0.403 ± 0.012	0.231CysPhe: 0.231 ± 0.008	0.977CysGly: 0.977 ± 0.022	0.195CysHis: 0.195 ± 0.009	0.158CysIle: 0.158 ± 0.008	0.12CysLys: 0.12 ± 0.008	0.785CysLeu: 0.785 ± 0.018	0.112CysMet: 0.112 ± 0.007	0.142CysAsn: 0.142 ± 0.008	0.496CysPro: 0.496 ± 0.014	0.16CysGln: 0.16 ± 0.008	0.608CysArg: 0.608 ± 0.019	0.466CysSer: 0.466 ± 0.013	0.517CysThr: 0.517 ± 0.014	0.717CysVal: 0.717 ± 0.018	0.136CysTrp: 0.136 ± 0.008	0.155CysTyr: 0.155 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.632AspAla: 7.632 ± 0.067	0.422AspCys: 0.422 ± 0.013	3.622AspAsp: 3.622 ± 0.041	3.898AspGlu: 3.898 ± 0.046	1.68AspPhe: 1.68 ± 0.026	6.617AspGly: 6.617 ± 0.063	1.447AspHis: 1.447 ± 0.029	1.805AspIle: 1.805 ± 0.026	1.222AspLys: 1.222 ± 0.026	6.155AspLeu: 6.155 ± 0.056	0.861AspMet: 0.861 ± 0.018	0.906AspAsn: 0.906 ± 0.022	4.606AspPro: 4.606 ± 0.047	1.441AspGln: 1.441 ± 0.023	5.048AspArg: 5.048 ± 0.052	2.604AspSer: 2.604 ± 0.032	3.387AspThr: 3.387 ± 0.039	4.822AspVal: 4.822 ± 0.044	1.057AspTrp: 1.057 ± 0.022	1.112AspTyr: 1.112 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.302GluAla: 7.302 ± 0.071	0.375GluCys: 0.375 ± 0.013	2.968GluAsp: 2.968 ± 0.043	3.628GluGlu: 3.628 ± 0.048	1.475GluPhe: 1.475 ± 0.025	4.428GluGly: 4.428 ± 0.044	1.576GluHis: 1.576 ± 0.027	2.231GluIle: 2.231 ± 0.034	1.398GluLys: 1.398 ± 0.029	6.72GluLeu: 6.72 ± 0.077	0.856GluMet: 0.856 ± 0.022	1.077GluAsn: 1.077 ± 0.022	3.405GluPro: 3.405 ± 0.04	2.108GluGln: 2.108 ± 0.035	5.636GluArg: 5.636 ± 0.062	2.641GluSer: 2.641 ± 0.032	2.95GluThr: 2.95 ± 0.036	4.371GluVal: 4.371 ± 0.044	0.748GluTrp: 0.748 ± 0.018	1.103GluTyr: 1.103 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.626PheAla: 3.626 ± 0.04	0.281PheCys: 0.281 ± 0.011	1.974PheAsp: 1.974 ± 0.024	1.467PheGlu: 1.467 ± 0.028	0.867PhePhe: 0.867 ± 0.019	3.066PheGly: 3.066 ± 0.036	0.622PheHis: 0.622 ± 0.018	0.666PheIle: 0.666 ± 0.016	0.514PheLys: 0.514 ± 0.016	2.583PheLeu: 2.583 ± 0.039	0.424PheMet: 0.424 ± 0.013	0.554PheAsn: 0.554 ± 0.017	1.415PhePro: 1.415 ± 0.023	0.69PheGln: 0.69 ± 0.016	1.9PheArg: 1.9 ± 0.025	1.486PheSer: 1.486 ± 0.024	2.034PheThr: 2.034 ± 0.029	2.314PheVal: 2.314 ± 0.028	0.413PheTrp: 0.413 ± 0.013	0.584PheTyr: 0.584 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.251GlyAla: 11.251 ± 0.084	0.83GlyCys: 0.83 ± 0.02	5.379GlyAsp: 5.379 ± 0.055	5.115GlyGlu: 5.115 ± 0.048	2.952GlyPhe: 2.952 ± 0.036	9.137GlyGly: 9.137 ± 0.089	2.456GlyHis: 2.456 ± 0.037	3.329GlyIle: 3.329 ± 0.037	2.382GlyLys: 2.382 ± 0.039	9.444GlyLeu: 9.444 ± 0.066	1.931GlyMet: 1.931 ± 0.026	1.765GlyAsn: 1.765 ± 0.036	5.529GlyPro: 5.529 ± 0.061	2.559GlyGln: 2.559 ± 0.04	8.16GlyArg: 8.16 ± 0.058	5.764GlySer: 5.764 ± 0.057	6.941GlyThr: 6.941 ± 0.063	7.617GlyVal: 7.617 ± 0.053	1.717GlyTrp: 1.717 ± 0.025	2.257GlyTyr: 2.257 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.707HisAla: 2.707 ± 0.036	0.208HisCys: 0.208 ± 0.01	1.407HisAsp: 1.407 ± 0.026	1.287HisGlu: 1.287 ± 0.025	0.653HisPhe: 0.653 ± 0.016	2.486HisGly: 2.486 ± 0.034	0.676HisHis: 0.676 ± 0.019	0.647HisIle: 0.647 ± 0.016	0.357HisLys: 0.357 ± 0.012	2.387HisLeu: 2.387 ± 0.032	0.334HisMet: 0.334 ± 0.01	0.359HisAsn: 0.359 ± 0.011	1.819HisPro: 1.819 ± 0.028	0.617HisGln: 0.617 ± 0.015	2.143HisArg: 2.143 ± 0.031	1.009HisSer: 1.009 ± 0.022	1.345HisThr: 1.345 ± 0.026	1.791HisVal: 1.791 ± 0.029	0.381HisTrp: 0.381 ± 0.015	0.485HisTyr: 0.485 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.443IleAla: 4.443 ± 0.05	0.274IleCys: 0.274 ± 0.01	2.121IleAsp: 2.121 ± 0.029	1.872IleGlu: 1.872 ± 0.031	0.63IlePhe: 0.63 ± 0.019	3.513IleGly: 3.513 ± 0.044	0.577IleHis: 0.577 ± 0.015	0.783IleIle: 0.783 ± 0.022	0.682IleLys: 0.682 ± 0.018	2.226IleLeu: 2.226 ± 0.035	0.418IleMet: 0.418 ± 0.014	0.628IleAsn: 0.628 ± 0.017	1.611IlePro: 1.611 ± 0.027	0.683IleGln: 0.683 ± 0.017	2.105IleArg: 2.105 ± 0.03	1.589IleSer: 1.589 ± 0.025	2.189IleThr: 2.189 ± 0.035	2.652IleVal: 2.652 ± 0.035	0.365IleTrp: 0.365 ± 0.014	0.495IleTyr: 0.495 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.923LysAla: 2.923 ± 0.045	0.12LysCys: 0.12 ± 0.007	1.337LysAsp: 1.337 ± 0.027	1.166LysGlu: 1.166 ± 0.023	0.455LysPhe: 0.455 ± 0.014	1.908LysGly: 1.908 ± 0.038	0.42LysHis: 0.42 ± 0.015	0.841LysIle: 0.841 ± 0.021	0.821LysLys: 0.821 ± 0.027	1.958LysLeu: 1.958 ± 0.034	0.359LysMet: 0.359 ± 0.012	0.543LysAsn: 0.543 ± 0.017	1.267LysPro: 1.267 ± 0.024	0.646LysGln: 0.646 ± 0.017	1.352LysArg: 1.352 ± 0.028	1.155LysSer: 1.155 ± 0.025	1.269LysThr: 1.269 ± 0.029	1.894LysVal: 1.894 ± 0.033	0.283LysTrp: 0.283 ± 0.011	0.457LysTyr: 0.457 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	14.726LeuAla: 14.726 ± 0.101	0.846LeuCys: 0.846 ± 0.018	6.679LeuAsp: 6.679 ± 0.064	4.81LeuGlu: 4.81 ± 0.056	2.628LeuPhe: 2.628 ± 0.035	9.29LeuGly: 9.29 ± 0.077	2.285LeuHis: 2.285 ± 0.031	3.08LeuIle: 3.08 ± 0.037	1.985LeuLys: 1.985 ± 0.031	11.158LeuLeu: 11.158 ± 0.09	1.627LeuMet: 1.627 ± 0.029	1.636LeuAsn: 1.636 ± 0.028	6.365LeuPro: 6.365 ± 0.052	2.147LeuGln: 2.147 ± 0.029	8.659LeuArg: 8.659 ± 0.074	5.372LeuSer: 5.372 ± 0.041	6.788LeuThr: 6.788 ± 0.057	8.901LeuVal: 8.901 ± 0.079	1.363LeuTrp: 1.363 ± 0.027	1.884LeuTyr: 1.884 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.175MetAla: 2.175 ± 0.031	0.137MetCys: 0.137 ± 0.007	0.866MetAsp: 0.866 ± 0.019	0.76MetGlu: 0.76 ± 0.018	0.461MetPhe: 0.461 ± 0.013	1.306MetGly: 1.306 ± 0.026	0.356MetHis: 0.356 ± 0.011	0.636MetIle: 0.636 ± 0.02	0.427MetLys: 0.427 ± 0.012	1.616MetLeu: 1.616 ± 0.029	0.303MetMet: 0.303 ± 0.012	0.456MetAsn: 0.456 ± 0.013	1.124MetPro: 1.124 ± 0.02	0.434MetGln: 0.434 ± 0.013	1.406MetArg: 1.406 ± 0.024	1.314MetSer: 1.314 ± 0.022	1.533MetThr: 1.533 ± 0.029	1.233MetVal: 1.233 ± 0.027	0.207MetTrp: 0.207 ± 0.01	0.322MetTyr: 0.322 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.166AsnAla: 2.166 ± 0.03	0.176AsnCys: 0.176 ± 0.009	0.935AsnAsp: 0.935 ± 0.02	0.814AsnGlu: 0.814 ± 0.021	0.458AsnPhe: 0.458 ± 0.015	1.956AsnGly: 1.956 ± 0.035	0.403AsnHis: 0.403 ± 0.013	0.625AsnIle: 0.625 ± 0.016	0.396AsnLys: 0.396 ± 0.014	1.616AsnLeu: 1.616 ± 0.027	0.3AsnMet: 0.3 ± 0.011	0.429AsnAsn: 0.429 ± 0.016	1.269AsnPro: 1.269 ± 0.023	0.496AsnGln: 0.496 ± 0.015	1.259AsnArg: 1.259 ± 0.026	0.956AsnSer: 0.956 ± 0.023	1.15AsnThr: 1.15 ± 0.023	1.325AsnVal: 1.325 ± 0.029	0.297AsnTrp: 0.297 ± 0.012	0.395AsnTyr: 0.395 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.818ProAla: 8.818 ± 0.085	0.348ProCys: 0.348 ± 0.011	4.546ProAsp: 4.546 ± 0.052	4.385ProGlu: 4.385 ± 0.049	1.551ProPhe: 1.551 ± 0.024	7.03ProGly: 7.03 ± 0.061	1.379ProHis: 1.379 ± 0.023	1.119ProIle: 1.119 ± 0.022	1.109ProLys: 1.109 ± 0.023	5.362ProLeu: 5.362 ± 0.054	1.026ProMet: 1.026 ± 0.022	0.871ProAsn: 0.871 ± 0.02	3.523ProPro: 3.523 ± 0.056	1.581ProGln: 1.581 ± 0.03	4.162ProArg: 4.162 ± 0.053	3.411ProSer: 3.411 ± 0.043	3.082ProThr: 3.082 ± 0.039	5.637ProVal: 5.637 ± 0.047	0.898ProTrp: 0.898 ± 0.017	1.414ProTyr: 1.414 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.414GlnAla: 3.414 ± 0.042	0.168GlnCys: 0.168 ± 0.008	1.49GlnAsp: 1.49 ± 0.026	1.403GlnGlu: 1.403 ± 0.027	0.666GlnPhe: 0.666 ± 0.016	2.275GlnGly: 2.275 ± 0.03	0.62GlnHis: 0.62 ± 0.015	1.031GlnIle: 1.031 ± 0.023	0.624GlnLys: 0.624 ± 0.02	2.826GlnLeu: 2.826 ± 0.031	0.456GlnMet: 0.456 ± 0.013	0.487GlnAsn: 0.487 ± 0.016	1.52GlnPro: 1.52 ± 0.03	1.165GlnGln: 1.165 ± 0.036	2.207GlnArg: 2.207 ± 0.033	1.251GlnSer: 1.251 ± 0.024	1.256GlnThr: 1.256 ± 0.024	2.292GlnVal: 2.292 ± 0.033	0.45GlnTrp: 0.45 ± 0.015	0.588GlnTyr: 0.588 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	10.053ArgAla: 10.053 ± 0.076	0.628ArgCys: 0.628 ± 0.018	4.291ArgAsp: 4.291 ± 0.043	4.838ArgGlu: 4.838 ± 0.051	2.382ArgPhe: 2.382 ± 0.03	6.007ArgGly: 6.007 ± 0.053	2.204ArgHis: 2.204 ± 0.031	3.164ArgIle: 3.164 ± 0.037	1.627ArgLys: 1.627 ± 0.028	8.945ArgLeu: 8.945 ± 0.089	1.694ArgMet: 1.694 ± 0.026	1.341ArgAsn: 1.341 ± 0.023	5.277ArgPro: 5.277 ± 0.059	2.238ArgGln: 2.238 ± 0.034	7.938ArgArg: 7.938 ± 0.076	4.264ArgSer: 4.264 ± 0.039	5.641ArgThr: 5.641 ± 0.053	5.931ArgVal: 5.931 ± 0.058	1.415ArgTrp: 1.415 ± 0.026	1.787ArgTyr: 1.787 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	7.103SerAla: 7.103 ± 0.059	0.449SerCys: 0.449 ± 0.015	2.778SerAsp: 2.778 ± 0.032	2.435SerGlu: 2.435 ± 0.03	1.542SerPhe: 1.542 ± 0.022	6.39SerGly: 6.39 ± 0.066	1.022SerHis: 1.022 ± 0.02	1.362SerIle: 1.362 ± 0.024	1.06SerLys: 1.06 ± 0.021	4.904SerLeu: 4.904 ± 0.045	1.072SerMet: 1.072 ± 0.021	0.898SerAsn: 0.898 ± 0.021	3.323SerPro: 3.323 ± 0.04	1.218SerGln: 1.218 ± 0.023	3.87SerArg: 3.87 ± 0.044	3.087SerSer: 3.087 ± 0.045	3.169SerThr: 3.169 ± 0.045	4.471SerVal: 4.471 ± 0.044	0.953SerTrp: 0.953 ± 0.021	1.292SerTyr: 1.292 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	9.141ThrAla: 9.141 ± 0.071	0.462ThrCys: 0.462 ± 0.014	3.694ThrAsp: 3.694 ± 0.041	3.241ThrGlu: 3.241 ± 0.037	1.629ThrPhe: 1.629 ± 0.03	7.105ThrGly: 7.105 ± 0.061	1.196ThrHis: 1.196 ± 0.023	1.576ThrIle: 1.576 ± 0.029	1.139ThrLys: 1.139 ± 0.024	5.722ThrLeu: 5.722 ± 0.047	0.927ThrMet: 0.927 ± 0.02	0.974ThrAsn: 0.974 ± 0.022	4.114ThrPro: 4.114 ± 0.044	1.276ThrGln: 1.276 ± 0.026	4.073ThrArg: 4.073 ± 0.047	3.248ThrSer: 3.248 ± 0.044	3.929ThrThr: 3.929 ± 0.049	6.417ThrVal: 6.417 ± 0.054	0.949ThrTrp: 0.949 ± 0.021	1.375ThrTyr: 1.375 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.498ValAla: 10.498 ± 0.073	0.781ValCys: 0.781 ± 0.018	4.985ValAsp: 4.985 ± 0.046	4.778ValGlu: 4.778 ± 0.044	2.484ValPhe: 2.484 ± 0.031	6.468ValGly: 6.468 ± 0.054	2.042ValHis: 2.042 ± 0.032	2.675ValIle: 2.675 ± 0.037	1.768ValLys: 1.768 ± 0.031	9.658ValLeu: 9.658 ± 0.079	1.443ValMet: 1.443 ± 0.026	1.641ValAsn: 1.641 ± 0.026	5.524ValPro: 5.524 ± 0.051	2.096ValGln: 2.096 ± 0.031	7.44ValArg: 7.44 ± 0.058	4.495ValSer: 4.495 ± 0.043	5.84ValThr: 5.84 ± 0.053	8.032ValVal: 8.032 ± 0.075	1.179ValTrp: 1.179 ± 0.021	1.614ValTyr: 1.614 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.647TrpAla: 1.647 ± 0.025	0.159TrpCys: 0.159 ± 0.008	0.88TrpAsp: 0.88 ± 0.02	0.752TrpGlu: 0.752 ± 0.019	0.515TrpPhe: 0.515 ± 0.013	1.07TrpGly: 1.07 ± 0.019	0.373TrpHis: 0.373 ± 0.015	0.552TrpIle: 0.552 ± 0.016	0.384TrpLys: 0.384 ± 0.012	1.768TrpLeu: 1.768 ± 0.029	0.283TrpMet: 0.283 ± 0.011	0.451TrpAsn: 0.451 ± 0.015	0.79TrpPro: 0.79 ± 0.021	0.566TrpGln: 0.566 ± 0.014	1.377TrpArg: 1.377 ± 0.028	1.023TrpSer: 1.023 ± 0.021	1.1TrpThr: 1.1 ± 0.024	0.969TrpVal: 0.969 ± 0.02	0.347TrpTrp: 0.347 ± 0.011	0.398TrpTyr: 0.398 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.797TyrAla: 2.797 ± 0.031	0.174TyrCys: 0.174 ± 0.009	1.564TyrAsp: 1.564 ± 0.027	1.312TyrGlu: 1.312 ± 0.021	0.645TyrPhe: 0.645 ± 0.016	2.312TyrGly: 2.312 ± 0.03	0.38TyrHis: 0.38 ± 0.013	0.476TyrIle: 0.476 ± 0.015	0.412TyrLys: 0.412 ± 0.016	2.018TyrLeu: 2.018 ± 0.029	0.262TyrMet: 0.262 ± 0.012	0.403TyrAsn: 0.403 ± 0.014	1.058TyrPro: 1.058 ± 0.02	0.582TyrGln: 0.582 ± 0.015	1.844TyrArg: 1.844 ± 0.029	0.988TyrSer: 0.988 ± 0.021	1.222TyrThr: 1.222 ± 0.026	1.709TyrVal: 1.709 ± 0.028	0.354TyrTrp: 0.354 ± 0.012	0.465TyrTyr: 0.465 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8072 proteins (2648258 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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