Rubrivivax albus
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4847 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A437JZI6|A0A437JZI6_9BURK RND transporter OS=Rubrivivax albus OX=2499835 GN=ENE75_00575 PE=4 SV=1
MM1 pKa = 7.3 FKK3 pKa = 10.55 SIAAAAVLAATAIAPASSAHH23 pKa = 6.43 ALTMGDD29 pKa = 2.84 IAFTGFNADD38 pKa = 2.97 QDD40 pKa = 3.78 NWSVVALADD49 pKa = 3.51 IAPGTEE55 pKa = 4.37 IYY57 pKa = 10.71 FSDD60 pKa = 4.1 NEE62 pKa = 4.34 WNGTAFNDD70 pKa = 4.34 TNEE73 pKa = 4.16 HH74 pKa = 4.72 TMLWNSGAATIAAGTVVLFTEE95 pKa = 5.02 ISSSNFGVSVGNLSLASGGGSNFGLSASDD124 pKa = 3.41 EE125 pKa = 4.19 TLYY128 pKa = 10.99 AYY130 pKa = 10.82 LGTDD134 pKa = 2.89 AVTPTLFLAGFSTEE148 pKa = 4.09 SGTGNLTAAGLTLGATAVQVTNSADD173 pKa = 3.31 YY174 pKa = 11.17 AEE176 pKa = 4.54 YY177 pKa = 10.56 VGPRR181 pKa = 11.84 LGQATFGAYY190 pKa = 10.36 AMLVNDD196 pKa = 4.05 ASSWNVLVGGDD207 pKa = 3.66 QSALVPDD214 pKa = 4.38 TTAFSVTAVPEE225 pKa = 4.41 PGTYY229 pKa = 11.26 AMLLSGLLAIGTFVRR244 pKa = 11.84 RR245 pKa = 11.84 KK246 pKa = 9.49 GG247 pKa = 3.4
Molecular weight: 25.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.834
IPC_protein 3.795
Toseland 3.592
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.923
DTASelect 4.139
Thurlkill 3.656
EMBOSS 3.745
Sillero 3.923
Patrickios 0.896
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A437JYJ7|A0A437JYJ7_9BURK Uncharacterized protein OS=Rubrivivax albus OX=2499835 GN=ENE75_10100 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 7.79 TRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.79 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIKK32 pKa = 10.36 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.91 GRR39 pKa = 11.84 KK40 pKa = 8.86 RR41 pKa = 11.84 LAA43 pKa = 3.57
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4847
0
4847
1628097
37
5221
335.9
36.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.363 ± 0.054
0.896 ± 0.012
5.649 ± 0.028
4.897 ± 0.032
3.219 ± 0.019
8.755 ± 0.047
2.355 ± 0.018
3.53 ± 0.025
2.384 ± 0.032
11.033 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.267 ± 0.018
2.033 ± 0.023
5.754 ± 0.026
3.793 ± 0.024
7.839 ± 0.041
4.601 ± 0.034
5.146 ± 0.033
7.902 ± 0.033
1.683 ± 0.017
1.903 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here