Streptomyces nitrosporeus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6F920|A0A5J6F920_9ACTN Succinate--CoA ligase [ADP-forming] subunit beta OS=Streptomyces nitrosporeus OX=28894 GN=sucC PE=3 SV=1
MM1 pKa = 7.73SNPQQLRR8 pKa = 11.84YY9 pKa = 10.06SKK11 pKa = 9.49EE12 pKa = 4.17HH13 pKa = 5.91EE14 pKa = 4.19WLSAAEE20 pKa = 4.61DD21 pKa = 4.12GVATVGITEE30 pKa = 4.15YY31 pKa = 11.0AANALGDD38 pKa = 3.65VVYY41 pKa = 10.52AQLPAVGDD49 pKa = 3.73TVTAGEE55 pKa = 4.44TCGEE59 pKa = 4.13LEE61 pKa = 4.31STKK64 pKa = 10.61SVSDD68 pKa = 4.05LYY70 pKa = 11.58SPVTGEE76 pKa = 3.73VTAANQDD83 pKa = 3.78VVDD86 pKa = 5.36DD87 pKa = 4.32PSLVNSAPFEE97 pKa = 4.42GGWLFKK103 pKa = 11.09VRR105 pKa = 11.84VTEE108 pKa = 4.24EE109 pKa = 4.13PDD111 pKa = 3.54DD112 pKa = 4.35LLSADD117 pKa = 4.52GYY119 pKa = 9.44TEE121 pKa = 4.18FSGNN125 pKa = 3.27

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6FF24|A0A5J6FF24_9ACTN Phenylacetic acid degradation protein PaaN OS=Streptomyces nitrosporeus OX=28894 GN=paaN PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6361

0

6361

2105033

29

4849

330.9

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.768 ± 0.045

0.795 ± 0.009

5.905 ± 0.022

5.819 ± 0.028

2.699 ± 0.017

10.01 ± 0.031

2.246 ± 0.015

2.943 ± 0.018

2.06 ± 0.024

10.191 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.737 ± 0.012

1.666 ± 0.014

6.278 ± 0.029

2.57 ± 0.017

8.211 ± 0.037

5.028 ± 0.021

6.148 ± 0.028

8.392 ± 0.029

1.463 ± 0.012

2.07 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski