Amino acid dipepetide frequency for Streptomyces nitrosporeus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.003AlaAla: 22.003 ± 0.187
1.077AlaCys: 1.077 ± 0.027
8.174AlaAsp: 8.174 ± 0.077
8.786AlaGlu: 8.786 ± 0.1
3.515AlaPhe: 3.515 ± 0.04
14.977AlaGly: 14.977 ± 0.115
2.809AlaHis: 2.809 ± 0.04
2.861AlaIle: 2.861 ± 0.044
2.67AlaLys: 2.67 ± 0.051
14.476AlaLeu: 14.476 ± 0.119
2.51AlaMet: 2.51 ± 0.045
1.722AlaAsn: 1.722 ± 0.033
7.855AlaPro: 7.855 ± 0.092
3.454AlaGln: 3.454 ± 0.052
10.69AlaArg: 10.69 ± 0.101
5.951AlaSer: 5.951 ± 0.062
6.731AlaThr: 6.731 ± 0.066
12.756AlaVal: 12.756 ± 0.096
1.811AlaTrp: 1.811 ± 0.029
2.858AlaTyr: 2.858 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.132CysAla: 1.132 ± 0.023
0.091CysCys: 0.091 ± 0.007
0.454CysAsp: 0.454 ± 0.015
0.412CysGlu: 0.412 ± 0.016
0.221CysPhe: 0.221 ± 0.01
0.978CysGly: 0.978 ± 0.021
0.188CysHis: 0.188 ± 0.01
0.16CysIle: 0.16 ± 0.009
0.099CysLys: 0.099 ± 0.007
0.719CysLeu: 0.719 ± 0.02
0.124CysMet: 0.124 ± 0.008
0.129CysAsn: 0.129 ± 0.007
0.523CysPro: 0.523 ± 0.018
0.157CysGln: 0.157 ± 0.008
0.632CysArg: 0.632 ± 0.02
0.441CysSer: 0.441 ± 0.016
0.513CysThr: 0.513 ± 0.018
0.695CysVal: 0.695 ± 0.02
0.127CysTrp: 0.127 ± 0.008
0.16CysTyr: 0.16 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.564AspAla: 7.564 ± 0.071
0.433AspCys: 0.433 ± 0.016
3.456AspAsp: 3.456 ± 0.048
3.854AspGlu: 3.854 ± 0.047
1.66AspPhe: 1.66 ± 0.024
6.796AspGly: 6.796 ± 0.068
1.331AspHis: 1.331 ± 0.028
1.842AspIle: 1.842 ± 0.032
1.096AspLys: 1.096 ± 0.029
5.893AspLeu: 5.893 ± 0.054
0.841AspMet: 0.841 ± 0.021
0.926AspAsn: 0.926 ± 0.022
4.576AspPro: 4.576 ± 0.055
1.355AspGln: 1.355 ± 0.028
5.089AspArg: 5.089 ± 0.055
2.43AspSer: 2.43 ± 0.032
3.38AspThr: 3.38 ± 0.035
4.461AspVal: 4.461 ± 0.05
0.993AspTrp: 0.993 ± 0.021
1.075AspTyr: 1.075 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
7.795GluAla: 7.795 ± 0.081
0.362GluCys: 0.362 ± 0.012
3.159GluAsp: 3.159 ± 0.044
3.93GluGlu: 3.93 ± 0.056
1.395GluPhe: 1.395 ± 0.026
4.779GluGly: 4.779 ± 0.052
1.468GluHis: 1.468 ± 0.025
2.262GluIle: 2.262 ± 0.038
1.552GluLys: 1.552 ± 0.034
6.635GluLeu: 6.635 ± 0.065
0.876GluMet: 0.876 ± 0.021
1.051GluAsn: 1.051 ± 0.029
3.451GluPro: 3.451 ± 0.045
2.129GluGln: 2.129 ± 0.03
5.532GluArg: 5.532 ± 0.05
2.57GluSer: 2.57 ± 0.032
3.026GluThr: 3.026 ± 0.046
4.361GluVal: 4.361 ± 0.055
0.743GluTrp: 0.743 ± 0.019
1.111GluTyr: 1.111 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.642PheAla: 3.642 ± 0.043
0.272PheCys: 0.272 ± 0.011
1.886PheAsp: 1.886 ± 0.032
1.484PheGlu: 1.484 ± 0.029
0.856PhePhe: 0.856 ± 0.024
3.069PheGly: 3.069 ± 0.042
0.603PheHis: 0.603 ± 0.017
0.695PheIle: 0.695 ± 0.02
0.479PheLys: 0.479 ± 0.019
2.61PheLeu: 2.61 ± 0.04
0.409PheMet: 0.409 ± 0.014
0.53PheAsn: 0.53 ± 0.018
1.358PhePro: 1.358 ± 0.027
0.626PheGln: 0.626 ± 0.017
1.841PheArg: 1.841 ± 0.035
1.463PheSer: 1.463 ± 0.027
2.005PheThr: 2.005 ± 0.033
2.182PheVal: 2.182 ± 0.033
0.414PheTrp: 0.414 ± 0.013
0.562PheTyr: 0.562 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
12.11GlyAla: 12.11 ± 0.101
0.858GlyCys: 0.858 ± 0.021
5.456GlyAsp: 5.456 ± 0.059
5.579GlyGlu: 5.579 ± 0.055
2.947GlyPhe: 2.947 ± 0.042
9.762GlyGly: 9.762 ± 0.1
2.454GlyHis: 2.454 ± 0.034
3.354GlyIle: 3.354 ± 0.042
2.388GlyLys: 2.388 ± 0.044
9.713GlyLeu: 9.713 ± 0.081
2.058GlyMet: 2.058 ± 0.036
1.733GlyAsn: 1.733 ± 0.027
6.164GlyPro: 6.164 ± 0.079
2.652GlyGln: 2.652 ± 0.04
8.63GlyArg: 8.63 ± 0.071
5.639GlySer: 5.639 ± 0.06
7.073GlyThr: 7.073 ± 0.064
7.529GlyVal: 7.529 ± 0.064
1.667GlyTrp: 1.667 ± 0.03
2.334GlyTyr: 2.334 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.579HisAla: 2.579 ± 0.037
0.206HisCys: 0.206 ± 0.009
1.292HisAsp: 1.292 ± 0.025
1.209HisGlu: 1.209 ± 0.026
0.642HisPhe: 0.642 ± 0.017
2.421HisGly: 2.421 ± 0.036
0.685HisHis: 0.685 ± 0.019
0.7HisIle: 0.7 ± 0.019
0.305HisLys: 0.305 ± 0.014
2.377HisLeu: 2.377 ± 0.036
0.333HisMet: 0.333 ± 0.011
0.363HisAsn: 0.363 ± 0.014
1.776HisPro: 1.776 ± 0.031
0.592HisGln: 0.592 ± 0.017
2.251HisArg: 2.251 ± 0.037
0.957HisSer: 0.957 ± 0.021
1.371HisThr: 1.371 ± 0.022
1.59HisVal: 1.59 ± 0.028
0.351HisTrp: 0.351 ± 0.014
0.457HisTyr: 0.457 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.33IleAla: 4.33 ± 0.051
0.294IleCys: 0.294 ± 0.012
2.082IleAsp: 2.082 ± 0.035
1.885IleGlu: 1.885 ± 0.03
0.633IlePhe: 0.633 ± 0.019
3.495IleGly: 3.495 ± 0.047
0.56IleHis: 0.56 ± 0.018
0.788IleIle: 0.788 ± 0.021
0.66IleLys: 0.66 ± 0.023
2.229IleLeu: 2.229 ± 0.036
0.445IleMet: 0.445 ± 0.018
0.619IleAsn: 0.619 ± 0.019
1.644IlePro: 1.644 ± 0.029
0.671IleGln: 0.671 ± 0.018
2.204IleArg: 2.204 ± 0.034
1.595IleSer: 1.595 ± 0.031
2.101IleThr: 2.101 ± 0.035
2.393IleVal: 2.393 ± 0.034
0.35IleTrp: 0.35 ± 0.013
0.457IleTyr: 0.457 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.907LysAla: 2.907 ± 0.047
0.105LysCys: 0.105 ± 0.007
1.32LysAsp: 1.32 ± 0.03
1.209LysGlu: 1.209 ± 0.027
0.436LysPhe: 0.436 ± 0.015
1.801LysGly: 1.801 ± 0.039
0.411LysHis: 0.411 ± 0.014
0.816LysIle: 0.816 ± 0.023
0.866LysLys: 0.866 ± 0.035
1.894LysLeu: 1.894 ± 0.034
0.387LysMet: 0.387 ± 0.014
0.505LysAsn: 0.505 ± 0.018
1.276LysPro: 1.276 ± 0.034
0.626LysGln: 0.626 ± 0.02
1.345LysArg: 1.345 ± 0.028
1.06LysSer: 1.06 ± 0.026
1.211LysThr: 1.211 ± 0.031
1.773LysVal: 1.773 ± 0.035
0.24LysTrp: 0.24 ± 0.011
0.415LysTyr: 0.415 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
14.769LeuAla: 14.769 ± 0.115
0.855LeuCys: 0.855 ± 0.02
6.627LeuAsp: 6.627 ± 0.063
4.813LeuGlu: 4.813 ± 0.049
2.576LeuPhe: 2.576 ± 0.038
9.369LeuGly: 9.369 ± 0.079
2.263LeuHis: 2.263 ± 0.033
3.028LeuIle: 3.028 ± 0.042
1.885LeuLys: 1.885 ± 0.031
11.26LeuLeu: 11.26 ± 0.112
1.667LeuMet: 1.667 ± 0.03
1.571LeuAsn: 1.571 ± 0.028
6.511LeuPro: 6.511 ± 0.063
2.091LeuGln: 2.091 ± 0.031
8.641LeuArg: 8.641 ± 0.071
5.225LeuSer: 5.225 ± 0.047
6.911LeuThr: 6.911 ± 0.059
8.763LeuVal: 8.763 ± 0.072
1.26LeuTrp: 1.26 ± 0.028
1.83LeuTyr: 1.83 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.279MetAla: 2.279 ± 0.033
0.138MetCys: 0.138 ± 0.008
0.894MetAsp: 0.894 ± 0.021
0.818MetGlu: 0.818 ± 0.021
0.451MetPhe: 0.451 ± 0.015
1.308MetGly: 1.308 ± 0.026
0.333MetHis: 0.333 ± 0.011
0.647MetIle: 0.647 ± 0.018
0.429MetLys: 0.429 ± 0.016
1.654MetLeu: 1.654 ± 0.032
0.307MetMet: 0.307 ± 0.013
0.43MetAsn: 0.43 ± 0.015
1.124MetPro: 1.124 ± 0.023
0.425MetGln: 0.425 ± 0.015
1.473MetArg: 1.473 ± 0.028
1.346MetSer: 1.346 ± 0.024
1.533MetThr: 1.533 ± 0.026
1.251MetVal: 1.251 ± 0.025
0.204MetTrp: 0.204 ± 0.009
0.326MetTyr: 0.326 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.095AsnAla: 2.095 ± 0.029
0.16AsnCys: 0.16 ± 0.008
0.916AsnAsp: 0.916 ± 0.023
0.796AsnGlu: 0.796 ± 0.021
0.432AsnPhe: 0.432 ± 0.014
1.813AsnGly: 1.813 ± 0.036
0.371AsnHis: 0.371 ± 0.014
0.615AsnIle: 0.615 ± 0.019
0.385AsnLys: 0.385 ± 0.016
1.496AsnLeu: 1.496 ± 0.035
0.302AsnMet: 0.302 ± 0.01
0.396AsnAsn: 0.396 ± 0.015
1.284AsnPro: 1.284 ± 0.031
0.46AsnGln: 0.46 ± 0.017
1.256AsnArg: 1.256 ± 0.024
0.895AsnSer: 0.895 ± 0.022
1.1AsnThr: 1.1 ± 0.027
1.245AsnVal: 1.245 ± 0.024
0.266AsnTrp: 0.266 ± 0.011
0.378AsnTyr: 0.378 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
9.835ProAla: 9.835 ± 0.097
0.349ProCys: 0.349 ± 0.013
4.486ProAsp: 4.486 ± 0.051
4.433ProGlu: 4.433 ± 0.045
1.525ProPhe: 1.525 ± 0.025
7.845ProGly: 7.845 ± 0.081
1.314ProHis: 1.314 ± 0.027
0.978ProIle: 0.978 ± 0.024
1.081ProLys: 1.081 ± 0.027
5.494ProLeu: 5.494 ± 0.054
0.999ProMet: 0.999 ± 0.025
0.767ProAsn: 0.767 ± 0.019
3.735ProPro: 3.735 ± 0.061
1.68ProGln: 1.68 ± 0.038
4.159ProArg: 4.159 ± 0.048
3.247ProSer: 3.247 ± 0.046
2.671ProThr: 2.671 ± 0.034
5.842ProVal: 5.842 ± 0.067
0.884ProTrp: 0.884 ± 0.023
1.462ProTyr: 1.462 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.505GlnAla: 3.505 ± 0.048
0.149GlnCys: 0.149 ± 0.01
1.466GlnAsp: 1.466 ± 0.029
1.532GlnGlu: 1.532 ± 0.029
0.641GlnPhe: 0.641 ± 0.017
2.286GlnGly: 2.286 ± 0.038
0.608GlnHis: 0.608 ± 0.019
1.005GlnIle: 1.005 ± 0.021
0.578GlnLys: 0.578 ± 0.019
2.797GlnLeu: 2.797 ± 0.04
0.444GlnMet: 0.444 ± 0.015
0.488GlnAsn: 0.488 ± 0.017
1.523GlnPro: 1.523 ± 0.042
1.082GlnGln: 1.082 ± 0.033
2.142GlnArg: 2.142 ± 0.032
1.102GlnSer: 1.102 ± 0.025
1.229GlnThr: 1.229 ± 0.028
2.186GlnVal: 2.186 ± 0.034
0.396GlnTrp: 0.396 ± 0.016
0.538GlnTyr: 0.538 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
10.254ArgAla: 10.254 ± 0.103
0.628ArgCys: 0.628 ± 0.022
4.298ArgAsp: 4.298 ± 0.058
4.961ArgGlu: 4.961 ± 0.054
2.33ArgPhe: 2.33 ± 0.033
6.123ArgGly: 6.123 ± 0.06
2.064ArgHis: 2.064 ± 0.033
3.265ArgIle: 3.265 ± 0.045
1.68ArgLys: 1.68 ± 0.032
8.798ArgLeu: 8.798 ± 0.079
1.739ArgMet: 1.739 ± 0.031
1.395ArgAsn: 1.395 ± 0.027
5.268ArgPro: 5.268 ± 0.059
2.219ArgGln: 2.219 ± 0.03
7.863ArgArg: 7.863 ± 0.103
4.235ArgSer: 4.235 ± 0.048
5.956ArgThr: 5.956 ± 0.057
5.82ArgVal: 5.82 ± 0.059
1.38ArgTrp: 1.38 ± 0.026
1.836ArgTyr: 1.836 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.689SerAla: 6.689 ± 0.066
0.43SerCys: 0.43 ± 0.016
2.59SerAsp: 2.59 ± 0.039
2.334SerGlu: 2.334 ± 0.032
1.556SerPhe: 1.556 ± 0.032
6.227SerGly: 6.227 ± 0.065
0.999SerHis: 0.999 ± 0.025
1.257SerIle: 1.257 ± 0.026
0.979SerLys: 0.979 ± 0.026
4.848SerLeu: 4.848 ± 0.056
1.025SerMet: 1.025 ± 0.022
0.797SerAsn: 0.797 ± 0.022
3.241SerPro: 3.241 ± 0.044
1.191SerGln: 1.191 ± 0.023
3.802SerArg: 3.802 ± 0.04
2.849SerSer: 2.849 ± 0.048
3.016SerThr: 3.016 ± 0.045
4.389SerVal: 4.389 ± 0.048
0.879SerTrp: 0.879 ± 0.021
1.177SerTyr: 1.177 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
9.393ThrAla: 9.393 ± 0.082
0.427ThrCys: 0.427 ± 0.015
3.561ThrAsp: 3.561 ± 0.044
3.389ThrGlu: 3.389 ± 0.043
1.594ThrPhe: 1.594 ± 0.035
7.594ThrGly: 7.594 ± 0.07
1.198ThrHis: 1.198 ± 0.024
1.438ThrIle: 1.438 ± 0.026
1.01ThrLys: 1.01 ± 0.024
5.628ThrLeu: 5.628 ± 0.056
0.911ThrMet: 0.911 ± 0.019
0.885ThrAsn: 0.885 ± 0.022
4.063ThrPro: 4.063 ± 0.049
1.186ThrGln: 1.186 ± 0.026
3.962ThrArg: 3.962 ± 0.046
3.015ThrSer: 3.015 ± 0.038
3.715ThrThr: 3.715 ± 0.052
6.315ThrVal: 6.315 ± 0.065
0.856ThrTrp: 0.856 ± 0.021
1.339ThrTyr: 1.339 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
10.294ValAla: 10.294 ± 0.081
0.79ValCys: 0.79 ± 0.019
4.647ValAsp: 4.647 ± 0.05
4.695ValGlu: 4.695 ± 0.048
2.476ValPhe: 2.476 ± 0.041
6.122ValGly: 6.122 ± 0.054
2.059ValHis: 2.059 ± 0.033
2.712ValIle: 2.712 ± 0.037
1.718ValLys: 1.718 ± 0.043
9.707ValLeu: 9.707 ± 0.085
1.463ValMet: 1.463 ± 0.027
1.61ValAsn: 1.61 ± 0.031
5.627ValPro: 5.627 ± 0.056
1.974ValGln: 1.974 ± 0.032
7.434ValArg: 7.434 ± 0.065
4.402ValSer: 4.402 ± 0.045
5.644ValThr: 5.644 ± 0.056
7.793ValVal: 7.793 ± 0.074
1.139ValTrp: 1.139 ± 0.021
1.611ValTyr: 1.611 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.662TrpAla: 1.662 ± 0.033
0.153TrpCys: 0.153 ± 0.009
0.829TrpAsp: 0.829 ± 0.021
0.783TrpGlu: 0.783 ± 0.021
0.48TrpPhe: 0.48 ± 0.015
1.029TrpGly: 1.029 ± 0.024
0.354TrpHis: 0.354 ± 0.012
0.532TrpIle: 0.532 ± 0.017
0.384TrpLys: 0.384 ± 0.017
1.63TrpLeu: 1.63 ± 0.029
0.283TrpMet: 0.283 ± 0.011
0.38TrpAsn: 0.38 ± 0.014
0.74TrpPro: 0.74 ± 0.019
0.569TrpGln: 0.569 ± 0.017
1.272TrpArg: 1.272 ± 0.026
0.891TrpSer: 0.891 ± 0.021
1.052TrpThr: 1.052 ± 0.026
0.949TrpVal: 0.949 ± 0.026
0.33TrpTrp: 0.33 ± 0.013
0.329TrpTyr: 0.329 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.847TyrAla: 2.847 ± 0.038
0.178TyrCys: 0.178 ± 0.008
1.458TyrAsp: 1.458 ± 0.03
1.284TyrGlu: 1.284 ± 0.025
0.616TyrPhe: 0.616 ± 0.019
2.304TyrGly: 2.304 ± 0.038
0.386TyrHis: 0.386 ± 0.014
0.477TyrIle: 0.477 ± 0.015
0.361TyrLys: 0.361 ± 0.015
2.058TyrLeu: 2.058 ± 0.033
0.248TyrMet: 0.248 ± 0.013
0.368TyrAsn: 0.368 ± 0.014
1.045TyrPro: 1.045 ± 0.022
0.559TyrGln: 0.559 ± 0.019
1.894TyrArg: 1.894 ± 0.032
0.964TyrSer: 0.964 ± 0.021
1.243TyrThr: 1.243 ± 0.026
1.623TyrVal: 1.623 ± 0.029
0.341TyrTrp: 0.341 ± 0.012
0.445TyrTyr: 0.445 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6361 proteins (2105033 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski